Reviewed, UniProtKB/Swiss-Prot Q63BZ8 (SYI2_BACCZ)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase 2
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
    IleRS 2

Gene names

Name:	ileS2
Ordered Locus Names:	BCE33L1977

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003
Cofactor	Zinc By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1033	1033	Isoleucyl-tRNA synthetase 2 HAMAP MF_02003		PRO_0000098518
Regions
	Motif	47 – 57	11	"HIGH" region HAMAP MF_02003
	Motif	590 – 594	5	"KMSKS" region HAMAP MF_02003
Sites
	Binding site	593	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63BZ8-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 2AE9F189D2EBC158	FASTA	1,033	118,486
	10 20 30 40 50 60 MKKVDVKESA VGRETRIRKQ WNEQSIFEQS IQNREGAQSF VFYEGPPTAN GLPHVGHALG 70 80 90 100 110 120 RTIKDVVARY KTMAGYKVLR KAGWDTHGLP VELGVEKQLG ISGKHEIEEY GIEPFIKKCK 130 140 150 160 170 180 ESVFTYEKQW REFTESIGYW VDMDDPYVTL ENPYIESVWH ILGTIHEKGL LYKGHRVSPY 190 200 210 220 230 240 CPSCQTSLSS HEVAQGYKTV KDLSATVKFK VKDSENEYFL GWTTTPWTLP ANVALAVHPN 250 260 270 280 290 300 MEYVKAKQEG HVYIVAKERV QDVLKENYEV LSVHKGEELL NISYTAPFPM KEVTNGYRVI 310 320 330 340 350 360 GADFVTADSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEAVP FLKGKFVKDC 370 380 390 400 410 420 DVDIVRYLAK EGLLYHKEKY EHSYPHCWRC DSPLLYYAGE SWLIRTTAIK DTFLQNNDSV 430 440 450 460 470 480 TWYPDHMKHG RFGKFLENMV DWNISRNRYW GTPLNVWECE SCDHQFAPKS IDDLRKHSTK 490 500 510 520 530 540 ETQEDLELHK PYVDEVQVCC EKCGGTMTRT PEVIDVWFDS GSMPFAQYHY PFENKELFEE 550 560 570 580 590 600 QFPADVIAEG IDQTRGWFYS LLAVSALYTG KVPYKRVLSL GHVLDEEGQK MSKSKGNALD 610 620 630 640 650 660 PVDLVNQFGA DALRWALLVD SAPWNAKRFS ERTVLEAKSK FVDTLVNVYS FYVLYANLDE 670 680 690 700 710 720 YNPNETYDVK RTKLDEWVLS RLHSTTKKVR IALDDYQFTN AAREIAALVD EVSNWYVRRS 730 740 750 760 770 780 RNRFWESGMN AEKAAAYETL HDVLVTISKL IAPFTPFVAE DIHLNLTGSS VHLEDYPVVN 790 800 810 820 830 840 ESLLQPKLEA EMNAVLQVVE LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM 850 860 870 880 890 900 DELNVKAFHV ELDETKYTSY QLKLNFKTAG PKFGKNVNAV NGWLKQLSQD EVQNFVSTER 910 920 930 940 950 960 AVYEVASGEE IVVTTEDVLV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ EGVAREFIRA 970 980 990 1000 1010 1020 VQEYRKQLNL PVNLRVDVIL DTEEELQQTL TNHKELLEEN LLVKQFTFGH LTNEDDELSL 1030 GETKVRIKLS ATK

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000001 Genomic DNA. Translation: AAU18279.1.
RefSeq	YP_083569.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q63BZ8.
Genome annotation databases
GeneID	3024309.
GenomeReviews	Gene locus BCE33L1977 in contig CP000001_GR.
KEGG	bcz:BCZK1977.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q63BZ8.
OMA	SNWYVRR.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L1977-MON.
Family and domain databases
HAMAP	MF_02003. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI2_BACCZ

Accession

Primary (citable) accession number: Q63BZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)