Q63BV5 (TYSY_BACCZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BCE33L2020

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Thymidylate synthase HAMAP MF_00008		PRO_0000140922
Sites
	Active site	200	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63BV5 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 07A755BD803FE296	FASTA	318	36,866
	10 20 30 40 50 60 MKHAENEYLN LCRHVMEHGT KKEDRTGTGT VSVFGYQMRF DLSKGFPLLT TKRVPFRLVA 70 80 90 100 110 120 SELLWFMKGD TNIRYLLQHN NNIWNEWAFK SWVESDEYTG PDMTDFGLRS QQDEEFKVQY 130 140 150 160 170 180 DKQMELFKKN VLEDDDFSNK YGYLGDVYGK QWRAWKTTAG ETLDQLKDVI EMIKKTPDSR 190 200 210 220 230 240 RLIVSAWNPE DVPSMALPPC HTLFQFYVAD GKLSCQLYQR SGDIFLGIPF NIASYSLLTH 250 260 270 280 290 300 LIAHECGLEV GEFVHTIGDA HIYTNHFEQV EKQLAREPRP FPKLTLNPDV KSVFDFEMED 310 LTIEGYDPHP AIKAPVAV

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ZK / E33L.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000001 Genomic DNA. Translation: AAU18236.1.
RefSeq	YP_083612.1. NC_006274.1.
3D structure databases
ProteinModelPortal	Q63BV5.
SMR	Q63BV5. Positions 5-318.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63BV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000044983; EBBACP00000043884; EBBACG00000044974.
GeneID	3023372.
GenomeReviews	Gene locus BCE33L2020 in contig CP000001_GR.
KEGG	bcz:BCZK2020.
PATRIC	18887574. VBIBacCer95304_2126.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
GeneTree	EBGT00050000001560.
HOGENOM	HBG588098.
OMA	RMALAPC.
PhylomeDB	Q63BV5.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L2020-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 2 hits.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACCZ

Accession

Primary (citable) accession number: Q63BV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program