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Protein

Myeloid cell surface antigen CD33

Gene

Cd33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid (By similarity). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Sialic acidBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Myeloid cell surface antigen CD33
Alternative name(s):
Sialic acid-binding Ig-like lectin 3
Short name:
Siglec-3
CD_antigen: CD33
Gene namesi
Name:Cd33
Synonyms:Siglec3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:99440. Cd33.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini18 – 240223ExtracellularSequence AnalysisAdd
BLAST
Transmembranei241 – 26727HelicalSequence AnalysisAdd
BLAST
Topological domaini268 – 403136CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 403387Myeloid cell surface antigen CD33PRO_0000014879Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 169PROSITE-ProRule annotation
Disulfide bondi41 ↔ 100PROSITE-ProRule annotation
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi163 ↔ 212PROSITE-ProRule annotation
Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ63994.

PTM databases

PhosphoSiteiQ63994.

Expressioni

Gene expression databases

BgeeiQ63994.
CleanExiMM_CD33.
GenevisibleiQ63994. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004728.

Structurei

3D structure databases

ProteinModelPortaliQ63994.
SMRiQ63994. Positions 21-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 120104Ig-like V-typeAdd
BLAST
Domaini145 – 22884Ig-like C2-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG320441.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000236324.
InParanoidiQ63994.
KOiK06473.
OMAiWACERST.
OrthoDBiEOG73JKV5.
PhylomeDBiQ63994.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 33-B (identifier: Q63994-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLWPLPLFLL CAGSLAQDLE FQLVAPESVT VEEGLCVHVP CSVFYPSIKL
60 70 80 90 100
TLGPVTGSWL RKGVSLHEDS PVATSDPRQL VQKATQGRFQ LLGDPQKHDC
110 120 130 140 150
SLFIRDAQKN DTGMYFFRVV REPFVRYSYK KSQLSLHVTS LSRTPDIIIP
160 170 180 190 200
GTLEAGYPSN LTCSVPWACE QGTPPTFSWM STALTSLSSR TTDSSVLTFT
210 220 230 240 250
PQPQDHGTKL TCLVTFSGAG VTVERTIQLN VTRKSGQMRE LVLVAVGEAT
260 270 280 290 300
VKLLILGLCL VFLIVMFCRR KTTKLSVHMG CENPIKRQEA ITSYNHCLSP
310 320 330 340 350
TASDAVTPGC SIHRLISRTP RCTAILRIQD PYRRTHLRNR AVSTLRFPWI
360 370 380 390 400
SWEGSLRSTQ RSKCTKLCSP VKNLCPLWLP VDNSCIPLIP EWVMLLCVSL

TLS
Length:403
Mass (Da):44,824
Last modified:November 1, 1996 - v1
Checksum:iF1FE6D5C393F0FF1
GO
Isoform 33-A (identifier: Q63994-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     287-403: RQEAITSYNH...MLLCVSLTLS → AHQQDSKVHS...GGKPQEYSEI

Show »
Length:334
Mass (Da):36,951
Checksum:iA3EC9608B5AFE617
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei287 – 403117RQEAI…SLTLS → AHQQDSKVHSNPENPRPLQK DSPQEQSSVHTKISLDFMGG KPQEYSEI in isoform 33-A. 2 PublicationsVSP_002534Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71345 mRNA. Translation: AAB30842.1.
S71403 mRNA. Translation: AAB30843.2.
BC132379 mRNA. Translation: AAI32380.1.
CCDSiCCDS21173.1. [Q63994-2]
CCDS52224.1. [Q63994-1]
RefSeqiNP_001104528.1. NM_001111058.1. [Q63994-1]
NP_067268.1. NM_021293.3. [Q63994-2]
UniGeneiMm.140157.

Genome annotation databases

EnsembliENSMUST00000004728; ENSMUSP00000004728; ENSMUSG00000004609. [Q63994-1]
ENSMUST00000039861; ENSMUSP00000045458; ENSMUSG00000004609. [Q63994-2]
GeneIDi12489.
KEGGimmu:12489.
UCSCiuc009gmz.2. mouse. [Q63994-2]
uc009gna.1. mouse. [Q63994-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Siglec-3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71345 mRNA. Translation: AAB30842.1.
S71403 mRNA. Translation: AAB30843.2.
BC132379 mRNA. Translation: AAI32380.1.
CCDSiCCDS21173.1. [Q63994-2]
CCDS52224.1. [Q63994-1]
RefSeqiNP_001104528.1. NM_001111058.1. [Q63994-1]
NP_067268.1. NM_021293.3. [Q63994-2]
UniGeneiMm.140157.

3D structure databases

ProteinModelPortaliQ63994.
SMRiQ63994. Positions 21-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004728.

PTM databases

PhosphoSiteiQ63994.

Proteomic databases

PRIDEiQ63994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004728; ENSMUSP00000004728; ENSMUSG00000004609. [Q63994-1]
ENSMUST00000039861; ENSMUSP00000045458; ENSMUSG00000004609. [Q63994-2]
GeneIDi12489.
KEGGimmu:12489.
UCSCiuc009gmz.2. mouse. [Q63994-2]
uc009gna.1. mouse. [Q63994-1]

Organism-specific databases

CTDi945.
MGIiMGI:99440. Cd33.

Phylogenomic databases

eggNOGiNOG320441.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000236324.
InParanoidiQ63994.
KOiK06473.
OMAiWACERST.
OrthoDBiEOG73JKV5.
PhylomeDBiQ63994.
TreeFamiTF332441.

Miscellaneous databases

NextBioi281404.
PROiQ63994.
SOURCEiSearch...

Gene expression databases

BgeeiQ63994.
CleanExiMM_CD33.
GenevisibleiQ63994. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of two isoforms of the murine homolog of the myeloid CD33 antigen."
    Tchilian E.Z., Beverley P.C., Young B.D., Watt S.M.
    Blood 83:3188-3198(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 33-A AND 33-B).
    Strain: BALB/c.
    Tissue: Bone marrow.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 33-A).
    Tissue: Brain.

Entry informationi

Entry nameiCD33_MOUSE
AccessioniPrimary (citable) accession number: Q63994
Secondary accession number(s): A2RT59, Q63997
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.