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Protein

Myeloid cell surface antigen CD33

Gene

Cd33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid (By similarity). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Sialic acidBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Myeloid cell surface antigen CD33
Alternative name(s):
Sialic acid-binding Ig-like lectin 3
Short name:
Siglec-3
CD_antigen: CD33
Gene namesi
Name:Cd33
Synonyms:Siglec3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:99440. Cd33.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 240ExtracellularSequence analysisAdd BLAST223
Transmembranei241 – 267HelicalSequence analysisAdd BLAST27
Topological domaini268 – 403CytoplasmicSequence analysisAdd BLAST136

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000001487917 – 403Myeloid cell surface antigen CD33Add BLAST387

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 169PROSITE-ProRule annotation
Disulfide bondi41 ↔ 100PROSITE-ProRule annotation
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Glycosylationi160N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi163 ↔ 212PROSITE-ProRule annotation
Glycosylationi230N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ63994.
PeptideAtlasiQ63994.
PRIDEiQ63994.

PTM databases

PhosphoSitePlusiQ63994.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004609.
CleanExiMM_CD33.
ExpressionAtlasiQ63994. baseline and differential.
GenevisibleiQ63994. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004728.

Structurei

3D structure databases

ProteinModelPortaliQ63994.
SMRiQ63994.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 120Ig-like V-typeAdd BLAST104
Domaini145 – 228Ig-like C2-typeAdd BLAST84

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J98M. Eukaryota.
ENOG41117XT. LUCA.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000236324.
InParanoidiQ63994.
KOiK06473.
OMAiHEDSPVA.
OrthoDBiEOG091G0JE3.
PhylomeDBiQ63994.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
[Graphical view]
PfamiPF00047. ig. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 33-B (identifier: Q63994-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLWPLPLFLL CAGSLAQDLE FQLVAPESVT VEEGLCVHVP CSVFYPSIKL
60 70 80 90 100
TLGPVTGSWL RKGVSLHEDS PVATSDPRQL VQKATQGRFQ LLGDPQKHDC
110 120 130 140 150
SLFIRDAQKN DTGMYFFRVV REPFVRYSYK KSQLSLHVTS LSRTPDIIIP
160 170 180 190 200
GTLEAGYPSN LTCSVPWACE QGTPPTFSWM STALTSLSSR TTDSSVLTFT
210 220 230 240 250
PQPQDHGTKL TCLVTFSGAG VTVERTIQLN VTRKSGQMRE LVLVAVGEAT
260 270 280 290 300
VKLLILGLCL VFLIVMFCRR KTTKLSVHMG CENPIKRQEA ITSYNHCLSP
310 320 330 340 350
TASDAVTPGC SIHRLISRTP RCTAILRIQD PYRRTHLRNR AVSTLRFPWI
360 370 380 390 400
SWEGSLRSTQ RSKCTKLCSP VKNLCPLWLP VDNSCIPLIP EWVMLLCVSL

TLS
Length:403
Mass (Da):44,824
Last modified:November 1, 1996 - v1
Checksum:iF1FE6D5C393F0FF1
GO
Isoform 33-A (identifier: Q63994-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     287-403: RQEAITSYNH...MLLCVSLTLS → AHQQDSKVHS...GGKPQEYSEI

Show »
Length:334
Mass (Da):36,951
Checksum:iA3EC9608B5AFE617
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002534287 – 403RQEAI…SLTLS → AHQQDSKVHSNPENPRPLQK DSPQEQSSVHTKISLDFMGG KPQEYSEI in isoform 33-A. 2 PublicationsAdd BLAST117

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71345 mRNA. Translation: AAB30842.1.
S71403 mRNA. Translation: AAB30843.2.
BC132379 mRNA. Translation: AAI32380.1.
CCDSiCCDS21173.1. [Q63994-2]
CCDS52224.1. [Q63994-1]
RefSeqiNP_001104528.1. NM_001111058.1. [Q63994-1]
NP_067268.1. NM_021293.3. [Q63994-2]
UniGeneiMm.140157.

Genome annotation databases

EnsembliENSMUST00000004728; ENSMUSP00000004728; ENSMUSG00000004609. [Q63994-1]
ENSMUST00000039861; ENSMUSP00000045458; ENSMUSG00000004609. [Q63994-2]
ENSMUST00000205503; ENSMUSP00000146225; ENSMUSG00000004609. [Q63994-2]
GeneIDi12489.
KEGGimmu:12489.
UCSCiuc009gmz.2. mouse. [Q63994-2]
uc009gna.1. mouse. [Q63994-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Siglec-3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71345 mRNA. Translation: AAB30842.1.
S71403 mRNA. Translation: AAB30843.2.
BC132379 mRNA. Translation: AAI32380.1.
CCDSiCCDS21173.1. [Q63994-2]
CCDS52224.1. [Q63994-1]
RefSeqiNP_001104528.1. NM_001111058.1. [Q63994-1]
NP_067268.1. NM_021293.3. [Q63994-2]
UniGeneiMm.140157.

3D structure databases

ProteinModelPortaliQ63994.
SMRiQ63994.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004728.

PTM databases

PhosphoSitePlusiQ63994.

Proteomic databases

PaxDbiQ63994.
PeptideAtlasiQ63994.
PRIDEiQ63994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004728; ENSMUSP00000004728; ENSMUSG00000004609. [Q63994-1]
ENSMUST00000039861; ENSMUSP00000045458; ENSMUSG00000004609. [Q63994-2]
ENSMUST00000205503; ENSMUSP00000146225; ENSMUSG00000004609. [Q63994-2]
GeneIDi12489.
KEGGimmu:12489.
UCSCiuc009gmz.2. mouse. [Q63994-2]
uc009gna.1. mouse. [Q63994-1]

Organism-specific databases

CTDi945.
MGIiMGI:99440. Cd33.

Phylogenomic databases

eggNOGiENOG410J98M. Eukaryota.
ENOG41117XT. LUCA.
GeneTreeiENSGT00760000119139.
HOGENOMiHOG000236324.
InParanoidiQ63994.
KOiK06473.
OMAiHEDSPVA.
OrthoDBiEOG091G0JE3.
PhylomeDBiQ63994.
TreeFamiTF332441.

Miscellaneous databases

PROiQ63994.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004609.
CleanExiMM_CD33.
ExpressionAtlasiQ63994. baseline and differential.
GenevisibleiQ63994. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
[Graphical view]
PfamiPF00047. ig. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD33_MOUSE
AccessioniPrimary (citable) accession number: Q63994
Secondary accession number(s): A2RT59, Q63997
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.