Q63994 (CD33_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myeloid cell surface antigen CD33

Alternative name(s):
Sialic acid-binding Ig-like lectin 3
Short name=Siglec-3
CD_antigen=CD33

Gene names

Name:	Cd33
Synonyms:	Siglec3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid By similarity. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
Ligand	Lectin
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	external side of plasma membrane Inferred from sequence orthology PubMed 20660734. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 33-B (identifier: Q63994-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 33-A (identifier: Q63994-2)

The sequence of this isoform differs from the canonical sequence as follows:
     287-403: RQEAITSYNH...MLLCVSLTLS → AHQQDSKVHS...GGKPQEYSEI

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 403	387	Myeloid cell surface antigen CD33		PRO_0000014879
Regions
	Topological domain	18 – 240	223	Extracellular Potential
	Transmembrane	241 – 267	27	Helical; Potential
	Topological domain	268 – 403	136	Cytoplasmic Potential
	Domain	17 – 120	104	Ig-like V-type
	Domain	145 – 228	84	Ig-like C2-type
Sites
	Binding site	118	1	Sialic acid By similarity
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	160	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
	Disulfide bond	36 ↔ 169		By similarity
	Disulfide bond	41 ↔ 100		By similarity
	Disulfide bond	163 ↔ 212		By similarity
Natural variations
	Alternative sequence	287 – 403	117	RQEAI…SLTLS → AHQQDSKVHSNPENPRPLQK DSPQEQSSVHTKISLDFMGG KPQEYSEI in isoform 33-A.		VSP_002534

Sequences

	Sequence		Length	Mass (Da)
	Isoform 33-B [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F1FE6D5C393F0FF1	FASTA	403	44,824
	10 20 30 40 50 60 MLWPLPLFLL CAGSLAQDLE FQLVAPESVT VEEGLCVHVP CSVFYPSIKL TLGPVTGSWL 70 80 90 100 110 120 RKGVSLHEDS PVATSDPRQL VQKATQGRFQ LLGDPQKHDC SLFIRDAQKN DTGMYFFRVV 130 140 150 160 170 180 REPFVRYSYK KSQLSLHVTS LSRTPDIIIP GTLEAGYPSN LTCSVPWACE QGTPPTFSWM 190 200 210 220 230 240 STALTSLSSR TTDSSVLTFT PQPQDHGTKL TCLVTFSGAG VTVERTIQLN VTRKSGQMRE 250 260 270 280 290 300 LVLVAVGEAT VKLLILGLCL VFLIVMFCRR KTTKLSVHMG CENPIKRQEA ITSYNHCLSP 310 320 330 340 350 360 TASDAVTPGC SIHRLISRTP RCTAILRIQD PYRRTHLRNR AVSTLRFPWI SWEGSLRSTQ 370 380 390 400 RSKCTKLCSP VKNLCPLWLP VDNSCIPLIP EWVMLLCVSL TLS
	Isoform 33-A [UniParc]. Checksum: A3EC9608B5AFE617	FASTA	334	36,951
	10 20 30 40 50 60 MLWPLPLFLL CAGSLAQDLE FQLVAPESVT VEEGLCVHVP CSVFYPSIKL TLGPVTGSWL 70 80 90 100 110 120 RKGVSLHEDS PVATSDPRQL VQKATQGRFQ LLGDPQKHDC SLFIRDAQKN DTGMYFFRVV 130 140 150 160 170 180 REPFVRYSYK KSQLSLHVTS LSRTPDIIIP GTLEAGYPSN LTCSVPWACE QGTPPTFSWM 190 200 210 220 230 240 STALTSLSSR TTDSSVLTFT PQPQDHGTKL TCLVTFSGAG VTVERTIQLN VTRKSGQMRE 250 260 270 280 290 300 LVLVAVGEAT VKLLILGLCL VFLIVMFCRR KTTKLSVHMG CENPIKAHQQ DSKVHSNPEN 310 320 330 PRPLQKDSPQ EQSSVHTKIS LDFMGGKPQE YSEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of two isoforms of the murine homolog of the myeloid CD33 antigen." Tchilian E.Z., Beverley P.C., Young B.D., Watt S.M. Blood 83:3188-3198(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 33-A AND 33-B). Strain: BALB/c. Tissue: Bone marrow.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 33-A). Tissue: Brain.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Siglec-3

Cross-references

Sequence databases
EMBL GenBank DDBJ	S71345 mRNA. Translation: AAB30842.1. S71403 mRNA. Translation: AAB30843.2. BC132379 mRNA. Translation: AAI32380.1.
IPI	IPI00135892. IPI00230329.
RefSeq	NP_001104528.1. NM_001111058.1. NP_067268.1. NM_021293.3.
UniGene	Mm.140157.
3D structure databases
ProteinModelPortal	Q63994.
SMR	Q63994. Positions 21-232.
ModBase	Search...
PTM databases
PhosphoSite	Q63994.
Proteomic databases
PRIDE	Q63994.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000004728; ENSMUSP00000004728; ENSMUSG00000004609. ENSMUST00000039861; ENSMUSP00000045458; ENSMUSG00000004609.
GeneID	12489.
KEGG	mmu:12489.
UCSC	uc009gmy.2. mouse. uc012fjg.1. mouse.
Organism-specific databases
CTD	945.
MGI	MGI:99440. Cd33.
Phylogenomic databases
eggNOG	NOG320441.
GeneTree	ENSGT00560000076846.
HOGENOM	HOG000236324.
InParanoid	Q63994.
KO	K06473.
OMA	CEVREET.
OrthoDB	EOG4K6G5G.
Gene expression databases
Bgee	Q63994.
CleanEx	MM_CD33.
Genevestigator	Q63994.
GermOnline	ENSMUSG00000004609. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. [Graphical view]
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281404.
SOURCE	Search...

Entry information

Entry name

CD33_MOUSE

Accession

Primary (citable) accession number: Q63994
Secondary accession number(s): A2RT59, Q63997

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program