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Q63961 (EGLN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglin
Alternative name(s):
Cell surface MJ7/18 antigen
CD_antigen=CD105
Gene names
Name:Eng
Synonyms:Edg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length653 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells. Ref.7

Subunit structure

Homodimer that forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Interacts with GDF2 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Endoglin is restricted to endothelial cells in all tissues except bone marrow and is also found in stromal cells within the connective tissue of intestine, stomach, heart, skeletal muscle, uterus, ovary, oviduct, testis and thymus.

Ontologies

Keywords
   Biological processAngiogenesis
Cell adhesion
   Cellular componentMembrane
   DomainSignal
Transmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiogenesis

Inferred from mutant phenotype PubMed 10625534PubMed 12941632. Source: MGI

artery morphogenesis

Inferred from mutant phenotype PubMed 10348742. Source: BHF-UCL

bone development

Inferred from electronic annotation. Source: Ensembl

cell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

cell chemotaxis

Inferred from electronic annotation. Source: Ensembl

cell migration involved in endocardial cushion formation

Inferred from mutant phenotype PubMed 19703439. Source: MGI

cellular response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

central nervous system vasculogenesis

Inferred from electronic annotation. Source: Ensembl

chronological cell aging

Inferred from expression pattern PubMed 18974388. Source: BHF-UCL

detection of hypoxia

Inferred from electronic annotation. Source: Ensembl

extracellular matrix constituent secretion

Inferred from electronic annotation. Source: Ensembl

extracellular matrix disassembly

Inferred from electronic annotation. Source: Ensembl

heart development

Inferred from mutant phenotype PubMed 10562296PubMed 10625534. Source: MGI

heart looping

Inferred from mutant phenotype PubMed 10348742. Source: BHF-UCL

negative regulation of cell migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of nitric-oxide synthase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of pathway-restricted SMAD protein phosphorylation

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein autophosphorylation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

patterning of blood vessels

Inferred from mutant phenotype PubMed 10348742. Source: BHF-UCL

positive regulation of BMP signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Traceable author statement PubMed 20406889. Source: DFLAT

positive regulation of collagen biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of pathway-restricted SMAD protein phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of systemic arterial blood pressure

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

regulation of transforming growth factor beta receptor signaling pathway

Traceable author statement PubMed 10625534. Source: MGI

response to corticosteroid

Inferred from electronic annotation. Source: Ensembl

response to statin

Inferred from electronic annotation. Source: Ensembl

smooth muscle tissue development

Inferred from mutant phenotype PubMed 10348742. Source: BHF-UCL

transforming growth factor beta receptor signaling pathway

Inferred by curator Ref.2. Source: BHF-UCL

vasculogenesis

Inferred from mutant phenotype PubMed 10348742. Source: BHF-UCL

venous blood vessel morphogenesis

Inferred from mutant phenotype PubMed 10348742. Source: BHF-UCL

wound healing

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

endothelial microparticle

Inferred from direct assay PubMed 20978343. Source: BHF-UCL

external side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nucleus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functiongalactose binding

Inferred from electronic annotation. Source: Ensembl

glycosaminoglycan binding

Inferred from electronic annotation. Source: Ensembl

protein homodimerization activity

Inferred from physical interaction Ref.2. Source: BHF-UCL

transforming growth factor beta binding

Inferred from direct assay Ref.2. Source: BHF-UCL

transforming growth factor beta receptor, cytoplasmic mediator activity

Inferred from electronic annotation. Source: Ensembl

transforming growth factor beta-activated receptor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 653627Endoglin
PRO_0000021157

Regions

Topological domain27 – 581555Extracellular Potential
Transmembrane582 – 60625Helical; Potential
Topological domain607 – 65347Cytoplasmic Potential
Compositional bias336 – 574239Ser/Thr-rich

Amino acid modifications

Modified residue6411Phosphoserine; by TGFBR1 Ref.6
Modified residue6441Phosphoserine; by TGFBR1 Ref.6
Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation1351N-linked (GlcNAc...) Potential
Glycosylation2661N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict941Q → R in CAA54917. Ref.2
Sequence conflict2871V → D in AAB30196. Ref.1
Sequence conflict5721I → V in CAA54917. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q63961 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: AD9DD2F823FB06A1

FASTA65370,021
        10         20         30         40         50         60 
MDRGVLPLPI TLLFVIYSFV PTTGLAERVG CDLQPVDPTR GEVTFTTSQV SEGCVAQAAN 

        70         80         90        100        110        120 
AVREVHVLFL DFPGMLSHLE LTLQASKQNG TETQEVFLVL VSNKNVFVKF QAPEIPLHLA 

       130        140        150        160        170        180 
YDSSLVIFQG QPRVNITVLP SLTSRKQILD WAATKGAITS IAALDDPQSI VLQLGQDPKA 

       190        200        210        220        230        240 
PFLCLPEAHK DMGATLEWQP RAQTPVQSCR LEGVSGHKEA YILRILPGSE AGPRTVTVMM 

       250        260        270        280        290        300 
ELSCTSGDAI LILHGPPYVS WFIDINHSMQ ILTTGEYSVK IFPGSKVKGV ELPDTPQGLI 

       310        320        330        340        350        360 
AEARKLNASI VTSFVELPLV SNVSLRASSC GGVFQTTPAP VVTTPPKDTC SPVLLMSLIQ 

       370        380        390        400        410        420 
PKCGNQVMTL ALNKKHVQTL QCTITGLTFW DSSCQAEDTD DHLVLSSAYS SCGMKVTAHV 

       430        440        450        460        470        480 
VSNEVIISFP SGSPPLRKKV QCIDMDSLSF QLGLYLSPHF LQASNTIELG QQAFVQVSVS 

       490        500        510        520        530        540 
PLTSEVTVQL DSCHLDLGPE GDMVELIQSR TAKGSCVTLL SPSPEGDPRF SFLLRVYMVP 

       550        560        570        580        590        600 
TPTAGTLSCN LALRPSTLSQ EVYKTVSMRL NIVSPDLSGK GLVLPSVLGI TFGAFLIGAL 

       610        620        630        640        650 
LTAALWYIYS HTRGPSKREP VVAVAAPASS ESSSTNHSIG STQSTPCSTS SMA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand."
Ge A.Z., Butcher E.C.
Gene 138:201-206(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Molecular characterization and in situ localization of murine endoglin reveal that it is a transforming growth factor-beta binding protein of endothelial and stromal cells."
St Jacques S., Cymerman U., Pece N., Letarte M.
Endocrinology 134:2645-2657(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CD-1.
Tissue: Placenta.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
[6]"ALK5 phosphorylation of the endoglin cytoplasmic domain regulates Smad1/5/8 signaling and endothelial cell migration."
Ray B.N., Lee N.Y., How T., Blobe G.C.
Carcinogenesis 31:435-441(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-641 AND SER-644.
[7]"Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies."
Nolan-Stevaux O., Zhong W., Culp S., Shaffer K., Hoover J., Wickramasinghe D., Ruefli-Brasse A.
PLoS ONE 7:E50920-E50920(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S69407 mRNA. Translation: AAB30196.1.
X77952 mRNA. Translation: CAA54917.1.
AL772271 Genomic DNA. Translation: CAM16615.1.
CH466542 Genomic DNA. Translation: EDL08562.1.
BC029080 mRNA. Translation: AAH29080.1.
PIRI48341.
RefSeqNP_031958.2. NM_007932.2.
UniGeneMm.225297.

3D structure databases

ProteinModelPortalQ63961.
SMRQ63961. Positions 445-559.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47636N.
IntActQ63961. 6 interactions.
MINTMINT-4116867.

PTM databases

PhosphoSiteQ63961.

Proteomic databases

PaxDbQ63961.
PRIDEQ63961.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000009705; ENSMUSP00000009705; ENSMUSG00000026814.
GeneID13805.
KEGGmmu:13805.
UCSCuc008jgk.2. mouse.

Organism-specific databases

CTD2022.
MGIMGI:95392. Eng.

Phylogenomic databases

eggNOGNOG46276.
GeneTreeENSGT00530000063861.
HOGENOMHOG000112346.
HOVERGENHBG005573.
InParanoidQ8K100.
KOK06526.
OMAHCDLQPV.
OrthoDBEOG70S754.
TreeFamTF337375.

Gene expression databases

ArrayExpressQ63961.
BgeeQ63961.
CleanExMM_ENG.
GenevestigatorQ63961.

Family and domain databases

InterProIPR001507. ZP_dom.
[Graphical view]
PfamPF00100. Zona_pellucida. 2 hits.
[Graphical view]
SMARTSM00241. ZP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284580.
PROQ63961.
SOURCESearch...

Entry information

Entry nameEGLN_MOUSE
AccessionPrimary (citable) accession number: Q63961
Secondary accession number(s): Q61520, Q8K100
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot