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Protein

Endoglin

Gene

Eng

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells.1 Publication

GO - Molecular functioni

  1. galactose binding Source: MGI
  2. glycosaminoglycan binding Source: MGI
  3. protein homodimerization activity Source: BHF-UCL
  4. transforming growth factor beta-activated receptor activity Source: MGI
  5. transforming growth factor beta binding Source: BHF-UCL
  6. transforming growth factor beta receptor, cytoplasmic mediator activity Source: MGI
  7. type II transforming growth factor beta receptor binding Source: MGI
  8. type I transforming growth factor beta receptor binding Source: MGI

GO - Biological processi

  1. angiogenesis Source: MGI
  2. artery morphogenesis Source: BHF-UCL
  3. bone development Source: Ensembl
  4. cell adhesion Source: UniProtKB-KW
  5. cell chemotaxis Source: MGI
  6. cell migration Source: MGI
  7. cell migration involved in endocardial cushion formation Source: MGI
  8. cell motility Source: MGI
  9. cellular response to mechanical stimulus Source: Ensembl
  10. central nervous system vasculogenesis Source: MGI
  11. chronological cell aging Source: BHF-UCL
  12. detection of hypoxia Source: MGI
  13. extracellular matrix constituent secretion Source: Ensembl
  14. extracellular matrix disassembly Source: MGI
  15. heart development Source: MGI
  16. heart looping Source: BHF-UCL
  17. intracellular signal transduction Source: MGI
  18. negative regulation of cell migration Source: MGI
  19. negative regulation of endothelial cell proliferation Source: MGI
  20. negative regulation of nitric-oxide synthase activity Source: MGI
  21. negative regulation of pathway-restricted SMAD protein phosphorylation Source: MGI
  22. negative regulation of protein autophosphorylation Source: MGI
  23. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  24. patterning of blood vessels Source: BHF-UCL
  25. positive regulation of angiogenesis Source: DFLAT
  26. positive regulation of BMP signaling pathway Source: MGI
  27. positive regulation of collagen biosynthetic process Source: Ensembl
  28. positive regulation of pathway-restricted SMAD protein phosphorylation Source: MGI
  29. positive regulation of protein phosphorylation Source: MGI
  30. positive regulation of systemic arterial blood pressure Source: MGI
  31. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  32. regulation of transcription, DNA-templated Source: MGI
  33. regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  34. response to corticosteroid Source: Ensembl
  35. response to hypoxia Source: MGI
  36. response to statin Source: Ensembl
  37. smooth muscle tissue development Source: BHF-UCL
  38. transforming growth factor beta receptor signaling pathway Source: BHF-UCL
  39. vasculogenesis Source: BHF-UCL
  40. venous blood vessel morphogenesis Source: BHF-UCL
  41. wound healing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Angiogenesis, Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglin
Alternative name(s):
Cell surface MJ7/18 antigen
CD_antigen: CD105
Gene namesi
Name:Eng
Synonyms:Edg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95392. Eng.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 581555ExtracellularSequence AnalysisAdd
BLAST
Transmembranei582 – 60625HelicalSequence AnalysisAdd
BLAST
Topological domaini607 – 65347CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. cytoplasm Source: MGI
  3. endothelial microparticle Source: BHF-UCL
  4. external side of plasma membrane Source: MGI
  5. extracellular space Source: MGI
  6. focal adhesion Source: MGI
  7. integral component of membrane Source: UniProtKB-KW
  8. nucleoplasm Source: MGI
  9. receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 653627EndoglinPRO_0000021157Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi266 – 2661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Modified residuei641 – 6411Phosphoserine; by TGFBR11 Publication
Modified residuei644 – 6441Phosphoserine; by TGFBR11 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ63961.
PaxDbiQ63961.
PRIDEiQ63961.

PTM databases

PhosphoSiteiQ63961.

Expressioni

Tissue specificityi

Endoglin is restricted to endothelial cells in all tissues except bone marrow and is also found in stromal cells within the connective tissue of intestine, stomach, heart, skeletal muscle, uterus, ovary, oviduct, testis and thymus.

Gene expression databases

BgeeiQ63961.
CleanExiMM_ENG.
ExpressionAtlasiQ63961. baseline and differential.
GenevestigatoriQ63961.

Interactioni

Subunit structurei

Homodimer that forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Interacts with GDF2 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-47636N.
IntActiQ63961. 6 interactions.
MINTiMINT-4116867.

Structurei

3D structure databases

ProteinModelPortaliQ63961.
SMRiQ63961. Positions 445-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 574239Ser/Thr-richAdd
BLAST

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46276.
GeneTreeiENSGT00530000063861.
HOGENOMiHOG000112346.
HOVERGENiHBG005573.
InParanoidiQ63961.
KOiK06526.
OMAiHCDLQPV.
OrthoDBiEOG70S754.
TreeFamiTF337375.

Family and domain databases

InterProiIPR001507. ZP_dom.
[Graphical view]
PfamiPF00100. Zona_pellucida. 2 hits.
[Graphical view]
SMARTiSM00241. ZP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRGVLPLPI TLLFVIYSFV PTTGLAERVG CDLQPVDPTR GEVTFTTSQV
60 70 80 90 100
SEGCVAQAAN AVREVHVLFL DFPGMLSHLE LTLQASKQNG TETQEVFLVL
110 120 130 140 150
VSNKNVFVKF QAPEIPLHLA YDSSLVIFQG QPRVNITVLP SLTSRKQILD
160 170 180 190 200
WAATKGAITS IAALDDPQSI VLQLGQDPKA PFLCLPEAHK DMGATLEWQP
210 220 230 240 250
RAQTPVQSCR LEGVSGHKEA YILRILPGSE AGPRTVTVMM ELSCTSGDAI
260 270 280 290 300
LILHGPPYVS WFIDINHSMQ ILTTGEYSVK IFPGSKVKGV ELPDTPQGLI
310 320 330 340 350
AEARKLNASI VTSFVELPLV SNVSLRASSC GGVFQTTPAP VVTTPPKDTC
360 370 380 390 400
SPVLLMSLIQ PKCGNQVMTL ALNKKHVQTL QCTITGLTFW DSSCQAEDTD
410 420 430 440 450
DHLVLSSAYS SCGMKVTAHV VSNEVIISFP SGSPPLRKKV QCIDMDSLSF
460 470 480 490 500
QLGLYLSPHF LQASNTIELG QQAFVQVSVS PLTSEVTVQL DSCHLDLGPE
510 520 530 540 550
GDMVELIQSR TAKGSCVTLL SPSPEGDPRF SFLLRVYMVP TPTAGTLSCN
560 570 580 590 600
LALRPSTLSQ EVYKTVSMRL NIVSPDLSGK GLVLPSVLGI TFGAFLIGAL
610 620 630 640 650
LTAALWYIYS HTRGPSKREP VVAVAAPASS ESSSTNHSIG STQSTPCSTS

SMA
Length:653
Mass (Da):70,021
Last modified:July 27, 2011 - v2
Checksum:iAD9DD2F823FB06A1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941Q → R in CAA54917 (PubMed:8194490).Curated
Sequence conflicti287 – 2871V → D in AAB30196 (PubMed:8125301).Curated
Sequence conflicti572 – 5721I → V in CAA54917 (PubMed:8194490).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69407 mRNA. Translation: AAB30196.1.
X77952 mRNA. Translation: CAA54917.1.
AL772271 Genomic DNA. Translation: CAM16615.1.
CH466542 Genomic DNA. Translation: EDL08562.1.
BC029080 mRNA. Translation: AAH29080.1.
CCDSiCCDS15925.1.
PIRiI48341.
RefSeqiNP_031958.2. NM_007932.2.
UniGeneiMm.225297.

Genome annotation databases

EnsembliENSMUST00000009705; ENSMUSP00000009705; ENSMUSG00000026814.
GeneIDi13805.
KEGGimmu:13805.
UCSCiuc008jgk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69407 mRNA. Translation: AAB30196.1.
X77952 mRNA. Translation: CAA54917.1.
AL772271 Genomic DNA. Translation: CAM16615.1.
CH466542 Genomic DNA. Translation: EDL08562.1.
BC029080 mRNA. Translation: AAH29080.1.
CCDSiCCDS15925.1.
PIRiI48341.
RefSeqiNP_031958.2. NM_007932.2.
UniGeneiMm.225297.

3D structure databases

ProteinModelPortaliQ63961.
SMRiQ63961. Positions 445-559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47636N.
IntActiQ63961. 6 interactions.
MINTiMINT-4116867.

PTM databases

PhosphoSiteiQ63961.

Proteomic databases

MaxQBiQ63961.
PaxDbiQ63961.
PRIDEiQ63961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009705; ENSMUSP00000009705; ENSMUSG00000026814.
GeneIDi13805.
KEGGimmu:13805.
UCSCiuc008jgk.2. mouse.

Organism-specific databases

CTDi2022.
MGIiMGI:95392. Eng.

Phylogenomic databases

eggNOGiNOG46276.
GeneTreeiENSGT00530000063861.
HOGENOMiHOG000112346.
HOVERGENiHBG005573.
InParanoidiQ63961.
KOiK06526.
OMAiHCDLQPV.
OrthoDBiEOG70S754.
TreeFamiTF337375.

Miscellaneous databases

NextBioi284580.
PROiQ63961.
SOURCEiSearch...

Gene expression databases

BgeeiQ63961.
CleanExiMM_ENG.
ExpressionAtlasiQ63961. baseline and differential.
GenevestigatoriQ63961.

Family and domain databases

InterProiIPR001507. ZP_dom.
[Graphical view]
PfamiPF00100. Zona_pellucida. 2 hits.
[Graphical view]
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand."
    Ge A.Z., Butcher E.C.
    Gene 138:201-206(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Molecular characterization and in situ localization of murine endoglin reveal that it is a transforming growth factor-beta binding protein of endothelial and stromal cells."
    St Jacques S., Cymerman U., Pece N., Letarte M.
    Endocrinology 134:2645-2657(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  6. "ALK5 phosphorylation of the endoglin cytoplasmic domain regulates Smad1/5/8 signaling and endothelial cell migration."
    Ray B.N., Lee N.Y., How T., Blobe G.C.
    Carcinogenesis 31:435-441(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-641 AND SER-644.
  7. "Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies."
    Nolan-Stevaux O., Zhong W., Culp S., Shaffer K., Hoover J., Wickramasinghe D., Ruefli-Brasse A.
    PLoS ONE 7:E50920-E50920(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiEGLN_MOUSE
AccessioniPrimary (citable) accession number: Q63961
Secondary accession number(s): Q61520, Q8K100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.