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Q63961

- EGLN_MOUSE

UniProt

Q63961 - EGLN_MOUSE

Protein

Endoglin

Gene

Eng

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells.1 Publication

    GO - Molecular functioni

    1. galactose binding Source: Ensembl
    2. glycosaminoglycan binding Source: Ensembl
    3. protein binding Source: MGI
    4. protein homodimerization activity Source: BHF-UCL
    5. transforming growth factor beta-activated receptor activity Source: Ensembl
    6. transforming growth factor beta binding Source: BHF-UCL
    7. transforming growth factor beta receptor, cytoplasmic mediator activity Source: Ensembl

    GO - Biological processi

    1. angiogenesis Source: MGI
    2. artery morphogenesis Source: BHF-UCL
    3. bone development Source: Ensembl
    4. cell adhesion Source: UniProtKB-KW
    5. cell chemotaxis Source: Ensembl
    6. cell migration involved in endocardial cushion formation Source: MGI
    7. cellular response to mechanical stimulus Source: Ensembl
    8. central nervous system vasculogenesis Source: Ensembl
    9. chronological cell aging Source: BHF-UCL
    10. detection of hypoxia Source: Ensembl
    11. extracellular matrix constituent secretion Source: Ensembl
    12. extracellular matrix disassembly Source: Ensembl
    13. heart development Source: MGI
    14. heart looping Source: BHF-UCL
    15. negative regulation of cell migration Source: Ensembl
    16. negative regulation of endothelial cell proliferation Source: Ensembl
    17. negative regulation of nitric-oxide synthase activity Source: Ensembl
    18. negative regulation of pathway-restricted SMAD protein phosphorylation Source: Ensembl
    19. negative regulation of protein autophosphorylation Source: Ensembl
    20. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    21. patterning of blood vessels Source: BHF-UCL
    22. positive regulation of angiogenesis Source: DFLAT
    23. positive regulation of BMP signaling pathway Source: Ensembl
    24. positive regulation of collagen biosynthetic process Source: Ensembl
    25. positive regulation of pathway-restricted SMAD protein phosphorylation Source: Ensembl
    26. positive regulation of systemic arterial blood pressure Source: Ensembl
    27. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    28. regulation of transforming growth factor beta receptor signaling pathway Source: MGI
    29. response to corticosteroid Source: Ensembl
    30. response to statin Source: Ensembl
    31. smooth muscle tissue development Source: BHF-UCL
    32. transforming growth factor beta receptor signaling pathway Source: BHF-UCL
    33. vasculogenesis Source: BHF-UCL
    34. venous blood vessel morphogenesis Source: BHF-UCL
    35. wound healing Source: Ensembl

    Keywords - Biological processi

    Angiogenesis, Cell adhesion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglin
    Alternative name(s):
    Cell surface MJ7/18 antigen
    CD_antigen: CD105
    Gene namesi
    Name:Eng
    Synonyms:Edg
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:95392. Eng.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. endothelial microparticle Source: BHF-UCL
    3. external side of plasma membrane Source: Ensembl
    4. integral component of membrane Source: UniProtKB-KW
    5. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 653627EndoglinPRO_0000021157Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi266 – 2661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
    Modified residuei641 – 6411Phosphoserine; by TGFBR11 Publication
    Modified residuei644 – 6441Phosphoserine; by TGFBR11 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ63961.
    PaxDbiQ63961.
    PRIDEiQ63961.

    PTM databases

    PhosphoSiteiQ63961.

    Expressioni

    Tissue specificityi

    Endoglin is restricted to endothelial cells in all tissues except bone marrow and is also found in stromal cells within the connective tissue of intestine, stomach, heart, skeletal muscle, uterus, ovary, oviduct, testis and thymus.

    Gene expression databases

    ArrayExpressiQ63961.
    BgeeiQ63961.
    CleanExiMM_ENG.
    GenevestigatoriQ63961.

    Interactioni

    Subunit structurei

    Homodimer that forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Interacts with GDF2 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-47636N.
    IntActiQ63961. 6 interactions.
    MINTiMINT-4116867.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63961.
    SMRiQ63961. Positions 445-559.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 581555ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini607 – 65347CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei582 – 60625HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi336 – 574239Ser/Thr-richAdd
    BLAST

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46276.
    GeneTreeiENSGT00530000063861.
    HOGENOMiHOG000112346.
    HOVERGENiHBG005573.
    InParanoidiQ8K100.
    KOiK06526.
    OMAiHCDLQPV.
    OrthoDBiEOG70S754.
    TreeFamiTF337375.

    Family and domain databases

    InterProiIPR001507. ZP_dom.
    [Graphical view]
    PfamiPF00100. Zona_pellucida. 2 hits.
    [Graphical view]
    SMARTiSM00241. ZP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q63961-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRGVLPLPI TLLFVIYSFV PTTGLAERVG CDLQPVDPTR GEVTFTTSQV    50
    SEGCVAQAAN AVREVHVLFL DFPGMLSHLE LTLQASKQNG TETQEVFLVL 100
    VSNKNVFVKF QAPEIPLHLA YDSSLVIFQG QPRVNITVLP SLTSRKQILD 150
    WAATKGAITS IAALDDPQSI VLQLGQDPKA PFLCLPEAHK DMGATLEWQP 200
    RAQTPVQSCR LEGVSGHKEA YILRILPGSE AGPRTVTVMM ELSCTSGDAI 250
    LILHGPPYVS WFIDINHSMQ ILTTGEYSVK IFPGSKVKGV ELPDTPQGLI 300
    AEARKLNASI VTSFVELPLV SNVSLRASSC GGVFQTTPAP VVTTPPKDTC 350
    SPVLLMSLIQ PKCGNQVMTL ALNKKHVQTL QCTITGLTFW DSSCQAEDTD 400
    DHLVLSSAYS SCGMKVTAHV VSNEVIISFP SGSPPLRKKV QCIDMDSLSF 450
    QLGLYLSPHF LQASNTIELG QQAFVQVSVS PLTSEVTVQL DSCHLDLGPE 500
    GDMVELIQSR TAKGSCVTLL SPSPEGDPRF SFLLRVYMVP TPTAGTLSCN 550
    LALRPSTLSQ EVYKTVSMRL NIVSPDLSGK GLVLPSVLGI TFGAFLIGAL 600
    LTAALWYIYS HTRGPSKREP VVAVAAPASS ESSSTNHSIG STQSTPCSTS 650
    SMA 653
    Length:653
    Mass (Da):70,021
    Last modified:July 27, 2011 - v2
    Checksum:iAD9DD2F823FB06A1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti94 – 941Q → R in CAA54917. (PubMed:8194490)Curated
    Sequence conflicti287 – 2871V → D in AAB30196. (PubMed:8125301)Curated
    Sequence conflicti572 – 5721I → V in CAA54917. (PubMed:8194490)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69407 mRNA. Translation: AAB30196.1.
    X77952 mRNA. Translation: CAA54917.1.
    AL772271 Genomic DNA. Translation: CAM16615.1.
    CH466542 Genomic DNA. Translation: EDL08562.1.
    BC029080 mRNA. Translation: AAH29080.1.
    CCDSiCCDS15925.1.
    PIRiI48341.
    RefSeqiNP_031958.2. NM_007932.2.
    UniGeneiMm.225297.

    Genome annotation databases

    EnsembliENSMUST00000009705; ENSMUSP00000009705; ENSMUSG00000026814.
    GeneIDi13805.
    KEGGimmu:13805.
    UCSCiuc008jgk.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69407 mRNA. Translation: AAB30196.1 .
    X77952 mRNA. Translation: CAA54917.1 .
    AL772271 Genomic DNA. Translation: CAM16615.1 .
    CH466542 Genomic DNA. Translation: EDL08562.1 .
    BC029080 mRNA. Translation: AAH29080.1 .
    CCDSi CCDS15925.1.
    PIRi I48341.
    RefSeqi NP_031958.2. NM_007932.2.
    UniGenei Mm.225297.

    3D structure databases

    ProteinModelPortali Q63961.
    SMRi Q63961. Positions 445-559.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47636N.
    IntActi Q63961. 6 interactions.
    MINTi MINT-4116867.

    PTM databases

    PhosphoSitei Q63961.

    Proteomic databases

    MaxQBi Q63961.
    PaxDbi Q63961.
    PRIDEi Q63961.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000009705 ; ENSMUSP00000009705 ; ENSMUSG00000026814 .
    GeneIDi 13805.
    KEGGi mmu:13805.
    UCSCi uc008jgk.2. mouse.

    Organism-specific databases

    CTDi 2022.
    MGIi MGI:95392. Eng.

    Phylogenomic databases

    eggNOGi NOG46276.
    GeneTreei ENSGT00530000063861.
    HOGENOMi HOG000112346.
    HOVERGENi HBG005573.
    InParanoidi Q8K100.
    KOi K06526.
    OMAi HCDLQPV.
    OrthoDBi EOG70S754.
    TreeFami TF337375.

    Miscellaneous databases

    NextBioi 284580.
    PROi Q63961.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q63961.
    Bgeei Q63961.
    CleanExi MM_ENG.
    Genevestigatori Q63961.

    Family and domain databases

    InterProi IPR001507. ZP_dom.
    [Graphical view ]
    Pfami PF00100. Zona_pellucida. 2 hits.
    [Graphical view ]
    SMARTi SM00241. ZP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand."
      Ge A.Z., Butcher E.C.
      Gene 138:201-206(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Molecular characterization and in situ localization of murine endoglin reveal that it is a transforming growth factor-beta binding protein of endothelial and stromal cells."
      St Jacques S., Cymerman U., Pece N., Letarte M.
      Endocrinology 134:2645-2657(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CD-1.
      Tissue: Placenta.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    6. "ALK5 phosphorylation of the endoglin cytoplasmic domain regulates Smad1/5/8 signaling and endothelial cell migration."
      Ray B.N., Lee N.Y., How T., Blobe G.C.
      Carcinogenesis 31:435-441(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-641 AND SER-644.
    7. "Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies."
      Nolan-Stevaux O., Zhong W., Culp S., Shaffer K., Hoover J., Wickramasinghe D., Ruefli-Brasse A.
      PLoS ONE 7:E50920-E50920(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiEGLN_MOUSE
    AccessioniPrimary (citable) accession number: Q63961
    Secondary accession number(s): Q61520, Q8K100
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3