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Q63959 (KCNC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily C member 3
Alternative name(s):
KSHIIID
Voltage-gated potassium channel subunit Kv3.3
Gene names
Name:Kcnc3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length769 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Subunit structure

Heterotetramer of potassium channel proteins By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

The KV3.3B isoform ishighly enriched in the brain, particularly in the cerebellum, where its expression is confined to Purkinje cells and deep cerebellar nuclei. Isoform KV33A is not expressed in cerebellum.

Developmental stage

Expression of KV3.3B begins in cerebellar Purkinje cells between postnatal day 8 (P8) and P10 and continues through adulthood.

Domain

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

Sequence similarities

Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.3/KCNC3 sub-subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform KV3.3B (identifier: Q63959-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform KV3.3A (identifier: Q63959-2)

The sequence of this isoform differs from the canonical sequence as follows:
     660-769: VDPRPNGDPA...RAPPTLPSIL → GEAGARTGGV...FGERDSETQV

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 769769Potassium voltage-gated channel subfamily C member 3
PRO_0000054056

Regions

Topological domain1 – 290290Cytoplasmic Potential
Transmembrane291 – 30919Helical; Name=Segment S1; Potential
Transmembrane351 – 37020Helical; Name=Segment S2; Potential
Topological domain371 – 3799Cytoplasmic Potential
Transmembrane380 – 39819Helical; Name=Segment S3; Potential
Transmembrane412 – 43423Helical; Voltage-sensor; Name=Segment S4; Potential
Topological domain435 – 44713Cytoplasmic Potential
Transmembrane448 – 46922Helical; Name=Segment S5; Potential
Transmembrane518 – 53922Helical; Name=Segment S6; Potential
Topological domain540 – 769230Cytoplasmic Potential
Motif503 – 5086Selectivity filter By similarity
Compositional bias41 – 444Poly-Gln
Compositional bias82 – 865Poly-Gly
Compositional bias229 – 2346Poly-Gly
Compositional bias577 – 58711Poly-Pro
Compositional bias596 – 5994Poly-Pro
Compositional bias669 – 6746Poly-Ala

Amino acid modifications

Glycosylation3201N-linked (GlcNAc...) Potential
Glycosylation3361N-linked (GlcNAc...) Potential
Glycosylation4831N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence660 – 769110VDPRP…LPSIL → GEAGARTGGVGRSGGKVAGL EGMQGQGFLGSGRVGWEATA DRKDKWLEGWTPGTKSHRNR IPRTRACEHGFQPGCSQRPL VRSSRVRIFGERDSETQV in isoform KV3.3A.
VSP_001021

Experimental info

Sequence conflict131R → C in AAC60679. Ref.1
Sequence conflict51 – 522AA → GP in AAC60679. Ref.1
Sequence conflict551A → G in AAC60679. Ref.1
Sequence conflict2561G → D in CAA43209. Ref.3
Sequence conflict257 – 2582PA → LP in AAC60679. Ref.1
Sequence conflict260 – 2667GAGGAGG → ARAAGA in CAA43209. Ref.3
Sequence conflict5211A → G in AAC60679. Ref.1
Sequence conflict5211A → G in CAA43209. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform KV3.3B [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: C910236DE8AF04C9

FASTA76982,143
        10         20         30         40         50         60 
MLSSVCVWSF RGRQGTGKQQ PQPVPTPQPP ESSPPPLPPP QQQQCSQPGT AASPAVPAFL 

        70         80         90        100        110        120 
RPGGRRAEPC PGLPAVAMGR HGGGGGDSGK IVINVGGVRH ETYRSTLRTL PGTRLAGLTE 

       130        140        150        160        170        180 
PEAAARFDYD PGTDEFFFDR HPGVFAYVLN YYRTGKLHCP ADVCGPLFEE ELGFWGIDET 

       190        200        210        220        230        240 
DVEACCWMTY RQHRDAEEAL DSFEAPDSSA NANANAGGAH DAGLDDEAGA GGGGLDGAGG 

       250        260        270        280        290        300 
ELKRLCFQDA GGGAGGPAGG AGGAGGTWWR RWQPRVWALF EDPYSSRAAR YVAFASLFFI 

       310        320        330        340        350        360 
LISITTFCLE THEGFIHISN KTVTQASPIP GAPPENITNV EVETEPFLTY VEGVCVVWFT 

       370        380        390        400        410        420 
FEFLMRVTFC PDKVEFLKSS LNIIDCVAIL PFYLEVGLSG LSSKAAKDVL GFLRVVRFVR 

       430        440        450        460        470        480 
ILRIFKLTRH FVGLRVLGHT LRASTNEFLL LIIFLALGVL IFATMIYYAE RIGADPDDIL 

       490        500        510        520        530        540 
GSNHTYFKNI PIGFWWAVVT MTTLGYGDMY PKTWSGMLVG ALCALAGVLT IAMPVPVIVN 

       550        560        570        580        590        600 
NFGMYYSLAM AKQKLPKKKN KHIPRPPQPG SPNYCKPDPP PPPPPHPHHG SGGISPPPPI 

       610        620        630        640        650        660 
TPPSMGVNVA GAYPPGPHTH PGLLRGGAGG LGIMGLPPLP APGEPCPLAQ EEVIETNRAV 

       670        680        690        700        710        720 
DPRPNGDPAA AALAHEDCPA IDQPAMSPED KSPITPGSRG RYSRDRACFL VTDYAPSPDG 

       730        740        750        760 
SIRKGYEKSR SLSSIVGLSG VSLRLAPLAT PPGSPRATRR APPTLPSIL 

« Hide

Isoform KV3.3A [UniParc].

Checksum: E948AE4B942F23F6
Show »

FASTA75781,190

References

« Hide 'large scale' references
[1]"Kv3.3b: a novel Shaw type potassium channel expressed in terminally differentiated cerebellar Purkinje cells and deep cerebellar nuclei."
Goldman-Wohl D.S., Chan E., Baird D., Heintz N.
J. Neurosci. 14:511-522(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM KV3.3B).
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Genomic organization, nucleotide sequence, and cellular distribution of a Shaw-related potassium channel gene, Kv3.3, and mapping of Kv3.3 and Kv3.4 to human chromosomes 19 and 1."
Ghanshani S., Pak M., McPherson J.D., Strong M., Dethlefs B., Wasmuth J.J., Salkoff L.A., Gutman G.A., Chandy G.K.
Genomics 12:190-196(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 78-769 (ISOFORM KV3.3A).
Strain: AKR/J.
[4]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S69381 mRNA. Translation: AAC60679.1.
AC157653 Genomic DNA. No translation available.
X60796, X60797 Genomic DNA. Translation: CAA43209.1.
PIRA42073.
UniGeneMm.40312.

3D structure databases

ProteinModelPortalQ63959.
SMRQ63959. Positions 1-51, 88-547.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ63959.

Proteomic databases

PaxDbQ63959.
PRIDEQ63959.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:96669. Kcnc3.

Phylogenomic databases

eggNOGNOG280627.
HOGENOMHOG000231012.
HOVERGENHBG105862.
InParanoidQ63959.

Gene expression databases

GenevestigatorQ63959.

Family and domain databases

Gene3D1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003974. K_chnl_volt-dep_Kv3.
IPR005404. K_chnl_volt-dep_Kv3.3.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERPTHR11537. PTHR11537. 1 hit.
PfamPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSPR00169. KCHANNEL.
PR01582. KV33CHANNEL.
PR01491. KVCHANNEL.
PR01498. SHAWCHANNEL.
SMARTSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
ProtoNetSearch...

Other

PROQ63959.
SOURCESearch...

Entry information

Entry nameKCNC3_MOUSE
AccessionPrimary (citable) accession number: Q63959
Secondary accession number(s): E9Q5E5, Q62088
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: January 22, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot