Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q63959

- KCNC3_MOUSE

UniProt

Q63959 - KCNC3_MOUSE

Protein

Potassium voltage-gated channel subfamily C member 3

Gene

Kcnc3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

    GO - Molecular functioni

    1. delayed rectifier potassium channel activity Source: RefGenome

    GO - Biological processi

    1. protein homooligomerization Source: InterPro
    2. regulation of neurotransmitter secretion Source: MGI

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily C member 3
    Alternative name(s):
    KSHIIID
    Voltage-gated potassium channel subunit Kv3.3
    Gene namesi
    Name:Kcnc3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:96669. Kcnc3.

    Subcellular locationi

    GO - Cellular componenti

    1. axolemma Source: MGI
    2. axon terminus Source: MGI
    3. dendrite membrane Source: MGI
    4. neuromuscular junction Source: MGI
    5. neuronal cell body membrane Source: MGI
    6. voltage-gated potassium channel complex Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 769769Potassium voltage-gated channel subfamily C member 3PRO_0000054056Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ63959.
    PRIDEiQ63959.

    PTM databases

    PhosphoSiteiQ63959.

    Expressioni

    Tissue specificityi

    The KV3.3B isoform is highly enriched in the brain, particularly in the cerebellum, where its expression is confined to Purkinje cells and deep cerebellar nuclei. Isoform KV3.3A is not expressed in cerebellum.

    Developmental stagei

    Expression of KV3.3B begins in cerebellar Purkinje cells between postnatal day 8 (P8) and P10 and continues through adulthood.

    Gene expression databases

    GenevestigatoriQ63959.

    Interactioni

    Subunit structurei

    Heterotetramer of potassium channel proteins.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ63959.
    SMRiQ63959. Positions 90-194, 272-546.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 290290CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini371 – 3799CytoplasmicSequence Analysis
    Topological domaini435 – 44713CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini540 – 769230CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei291 – 30919Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei351 – 37020Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei380 – 39819Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei412 – 43423Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei448 – 46922Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei518 – 53922Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi503 – 5086Selectivity filterBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi41 – 444Poly-Gln
    Compositional biasi82 – 865Poly-Gly
    Compositional biasi229 – 2346Poly-Gly
    Compositional biasi577 – 58711Poly-ProAdd
    BLAST
    Compositional biasi596 – 5994Poly-Pro
    Compositional biasi669 – 6746Poly-Ala

    Domaini

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG280627.
    HOGENOMiHOG000231012.
    HOVERGENiHBG105862.
    InParanoidiQ63959.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003974. K_chnl_volt-dep_Kv3.
    IPR005404. K_chnl_volt-dep_Kv3.3.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01582. KV33CHANNEL.
    PR01491. KVCHANNEL.
    PR01498. SHAWCHANNEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform KV3.3B (identifier: Q63959-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLSSVCVWSF RGRQGTGKQQ PQPVPTPQPP ESSPPPLPPP QQQQCSQPGT    50
    AASPAVPAFL RPGGRRAEPC PGLPAVAMGR HGGGGGDSGK IVINVGGVRH 100
    ETYRSTLRTL PGTRLAGLTE PEAAARFDYD PGTDEFFFDR HPGVFAYVLN 150
    YYRTGKLHCP ADVCGPLFEE ELGFWGIDET DVEACCWMTY RQHRDAEEAL 200
    DSFEAPDSSA NANANAGGAH DAGLDDEAGA GGGGLDGAGG ELKRLCFQDA 250
    GGGAGGPAGG AGGAGGTWWR RWQPRVWALF EDPYSSRAAR YVAFASLFFI 300
    LISITTFCLE THEGFIHISN KTVTQASPIP GAPPENITNV EVETEPFLTY 350
    VEGVCVVWFT FEFLMRVTFC PDKVEFLKSS LNIIDCVAIL PFYLEVGLSG 400
    LSSKAAKDVL GFLRVVRFVR ILRIFKLTRH FVGLRVLGHT LRASTNEFLL 450
    LIIFLALGVL IFATMIYYAE RIGADPDDIL GSNHTYFKNI PIGFWWAVVT 500
    MTTLGYGDMY PKTWSGMLVG ALCALAGVLT IAMPVPVIVN NFGMYYSLAM 550
    AKQKLPKKKN KHIPRPPQPG SPNYCKPDPP PPPPPHPHHG SGGISPPPPI 600
    TPPSMGVNVA GAYPPGPHTH PGLLRGGAGG LGIMGLPPLP APGEPCPLAQ 650
    EEVIETNRAV DPRPNGDPAA AALAHEDCPA IDQPAMSPED KSPITPGSRG 700
    RYSRDRACFL VTDYAPSPDG SIRKGYEKSR SLSSIVGLSG VSLRLAPLAT 750
    PPGSPRATRR APPTLPSIL 769
    Length:769
    Mass (Da):82,143
    Last modified:July 27, 2011 - v2
    Checksum:iC910236DE8AF04C9
    GO
    Isoform KV3.3A (identifier: Q63959-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         660-769: VDPRPNGDPA...RAPPTLPSIL → GEAGARTGGV...FGERDSETQV

    Show »
    Length:757
    Mass (Da):81,190
    Checksum:iE948AE4B942F23F6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131R → C in AAC60679. (PubMed:8301351)Curated
    Sequence conflicti51 – 522AA → GP in AAC60679. (PubMed:8301351)Curated
    Sequence conflicti55 – 551A → G in AAC60679. (PubMed:8301351)Curated
    Sequence conflicti256 – 2561G → D in CAA43209. (PubMed:1740329)Curated
    Sequence conflicti257 – 2582PA → LP in AAC60679. (PubMed:8301351)Curated
    Sequence conflicti260 – 2667GAGGAGG → ARAAGA in CAA43209. (PubMed:1740329)Curated
    Sequence conflicti521 – 5211A → G in AAC60679. (PubMed:8301351)Curated
    Sequence conflicti521 – 5211A → G in CAA43209. (PubMed:1740329)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei660 – 769110VDPRP…LPSIL → GEAGARTGGVGRSGGKVAGL EGMQGQGFLGSGRVGWEATA DRKDKWLEGWTPGTKSHRNR IPRTRACEHGFQPGCSQRPL VRSSRVRIFGERDSETQV in isoform KV3.3A. CuratedVSP_001021Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69381 mRNA. Translation: AAC60679.1.
    AC157653 Genomic DNA. No translation available.
    X60796, X60797 Genomic DNA. Translation: CAA43209.1.
    PIRiA42073.
    UniGeneiMm.40312.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69381 mRNA. Translation: AAC60679.1 .
    AC157653 Genomic DNA. No translation available.
    X60796 , X60797 Genomic DNA. Translation: CAA43209.1 .
    PIRi A42073.
    UniGenei Mm.40312.

    3D structure databases

    ProteinModelPortali Q63959.
    SMRi Q63959. Positions 90-194, 272-546.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q63959.

    Proteomic databases

    PaxDbi Q63959.
    PRIDEi Q63959.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:96669. Kcnc3.

    Phylogenomic databases

    eggNOGi NOG280627.
    HOGENOMi HOG000231012.
    HOVERGENi HBG105862.
    InParanoidi Q63959.

    Miscellaneous databases

    PROi Q63959.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q63959.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003974. K_chnl_volt-dep_Kv3.
    IPR005404. K_chnl_volt-dep_Kv3.3.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01582. KV33CHANNEL.
    PR01491. KVCHANNEL.
    PR01498. SHAWCHANNEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Kv3.3b: a novel Shaw type potassium channel expressed in terminally differentiated cerebellar Purkinje cells and deep cerebellar nuclei."
      Goldman-Wohl D.S., Chan E., Baird D., Heintz N.
      J. Neurosci. 14:511-522(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM KV3.3B).
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Genomic organization, nucleotide sequence, and cellular distribution of a Shaw-related potassium channel gene, Kv3.3, and mapping of Kv3.3 and Kv3.4 to human chromosomes 19 and 1."
      Ghanshani S., Pak M., McPherson J.D., Strong M., Dethlefs B., Wasmuth J.J., Salkoff L.A., Gutman G.A., Chandy G.K.
      Genomics 12:190-196(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 78-769 (ISOFORM KV3.3A).
      Strain: AKR/J.
    4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiKCNC3_MOUSE
    AccessioniPrimary (citable) accession number: Q63959
    Secondary accession number(s): E9Q5E5, Q62088
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3