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Protein

Potassium voltage-gated channel subfamily C member 3

Gene

Kcnc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily C member 3
Alternative name(s):
KSHIIID
Voltage-gated potassium channel subunit Kv3.3
Gene namesi
Name:Kcnc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96669. Kcnc3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 290290CytoplasmicSequence analysisAdd
BLAST
Transmembranei291 – 30919Helical; Name=Segment S1Sequence analysisAdd
BLAST
Transmembranei351 – 37020Helical; Name=Segment S2Sequence analysisAdd
BLAST
Topological domaini371 – 3799CytoplasmicSequence analysis
Transmembranei380 – 39819Helical; Name=Segment S3Sequence analysisAdd
BLAST
Transmembranei412 – 43423Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd
BLAST
Topological domaini435 – 44713CytoplasmicSequence analysisAdd
BLAST
Transmembranei448 – 46922Helical; Name=Segment S5Sequence analysisAdd
BLAST
Transmembranei518 – 53922Helical; Name=Segment S6Sequence analysisAdd
BLAST
Topological domaini540 – 769230CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • axolemma Source: MGI
  • axon terminus Source: MGI
  • dendrite membrane Source: MGI
  • integral component of membrane Source: GO_Central
  • neuromuscular junction Source: MGI
  • neuronal cell body membrane Source: MGI
  • plasma membrane Source: MGI
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 769769Potassium voltage-gated channel subfamily C member 3PRO_0000054056Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence analysis
Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence analysis
Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence analysis
Modified residuei687 – 6871PhosphoserineCombined sources
Modified residuei692 – 6921PhosphoserineCombined sources
Modified residuei731 – 7311PhosphoserineCombined sources
Modified residuei734 – 7341PhosphoserineCombined sources
Modified residuei742 – 7421PhosphoserineCombined sources
Modified residuei750 – 7501PhosphothreonineCombined sources
Modified residuei754 – 7541PhosphoserineCombined sources

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ63959.
PaxDbiQ63959.
PeptideAtlasiQ63959.
PRIDEiQ63959.

PTM databases

iPTMnetiQ63959.
PhosphoSiteiQ63959.

Expressioni

Tissue specificityi

The KV3.3B isoform is highly enriched in the brain, particularly in the cerebellum, where its expression is confined to Purkinje cells and deep cerebellar nuclei. Isoform KV3.3A is not expressed in cerebellum.

Developmental stagei

Expression of KV3.3B begins in cerebellar Purkinje cells between postnatal day 8 (P8) and P10 and continues through adulthood.

Interactioni

Subunit structurei

Homotetramer or heterotetramer. Heterotetramer with KCNC1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103540.

Structurei

3D structure databases

ProteinModelPortaliQ63959.
SMRiQ63959. Positions 90-194, 272-546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi503 – 5086Selectivity filterBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 444Poly-Gln
Compositional biasi82 – 865Poly-Gly
Compositional biasi229 – 2346Poly-Gly
Compositional biasi577 – 58711Poly-ProAdd
BLAST
Compositional biasi596 – 5994Poly-Pro
Compositional biasi669 – 6746Poly-Ala

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3713. Eukaryota.
COG1226. LUCA.
HOGENOMiHOG000231012.
HOVERGENiHBG105862.
InParanoidiQ63959.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003974. K_chnl_volt-dep_Kv3.
IPR005404. K_chnl_volt-dep_Kv3.3.
IPR021105. K_chnl_volt-dep_Kv3_ID.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 3 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11404. Potassium_chann. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01582. KV33CHANNEL.
PR01491. KVCHANNEL.
PR01498. SHAWCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform KV3.3B (identifier: Q63959-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSSVCVWSF RGRQGTGKQQ PQPVPTPQPP ESSPPPLPPP QQQQCSQPGT
60 70 80 90 100
AASPAVPAFL RPGGRRAEPC PGLPAVAMGR HGGGGGDSGK IVINVGGVRH
110 120 130 140 150
ETYRSTLRTL PGTRLAGLTE PEAAARFDYD PGTDEFFFDR HPGVFAYVLN
160 170 180 190 200
YYRTGKLHCP ADVCGPLFEE ELGFWGIDET DVEACCWMTY RQHRDAEEAL
210 220 230 240 250
DSFEAPDSSA NANANAGGAH DAGLDDEAGA GGGGLDGAGG ELKRLCFQDA
260 270 280 290 300
GGGAGGPAGG AGGAGGTWWR RWQPRVWALF EDPYSSRAAR YVAFASLFFI
310 320 330 340 350
LISITTFCLE THEGFIHISN KTVTQASPIP GAPPENITNV EVETEPFLTY
360 370 380 390 400
VEGVCVVWFT FEFLMRVTFC PDKVEFLKSS LNIIDCVAIL PFYLEVGLSG
410 420 430 440 450
LSSKAAKDVL GFLRVVRFVR ILRIFKLTRH FVGLRVLGHT LRASTNEFLL
460 470 480 490 500
LIIFLALGVL IFATMIYYAE RIGADPDDIL GSNHTYFKNI PIGFWWAVVT
510 520 530 540 550
MTTLGYGDMY PKTWSGMLVG ALCALAGVLT IAMPVPVIVN NFGMYYSLAM
560 570 580 590 600
AKQKLPKKKN KHIPRPPQPG SPNYCKPDPP PPPPPHPHHG SGGISPPPPI
610 620 630 640 650
TPPSMGVNVA GAYPPGPHTH PGLLRGGAGG LGIMGLPPLP APGEPCPLAQ
660 670 680 690 700
EEVIETNRAV DPRPNGDPAA AALAHEDCPA IDQPAMSPED KSPITPGSRG
710 720 730 740 750
RYSRDRACFL VTDYAPSPDG SIRKGYEKSR SLSSIVGLSG VSLRLAPLAT
760
PPGSPRATRR APPTLPSIL
Length:769
Mass (Da):82,143
Last modified:July 27, 2011 - v2
Checksum:iC910236DE8AF04C9
GO
Isoform KV3.3A (identifier: Q63959-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     660-769: VDPRPNGDPA...RAPPTLPSIL → GEAGARTGGV...FGERDSETQV

Show »
Length:757
Mass (Da):81,190
Checksum:iE948AE4B942F23F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131R → C in AAC60679 (PubMed:8301351).Curated
Sequence conflicti51 – 522AA → GP in AAC60679 (PubMed:8301351).Curated
Sequence conflicti55 – 551A → G in AAC60679 (PubMed:8301351).Curated
Sequence conflicti256 – 2561G → D in CAA43209 (PubMed:1740329).Curated
Sequence conflicti257 – 2582PA → LP in AAC60679 (PubMed:8301351).Curated
Sequence conflicti260 – 2667GAGGAGG → ARAAGA in CAA43209 (PubMed:1740329).Curated
Sequence conflicti521 – 5211A → G in AAC60679 (PubMed:8301351).Curated
Sequence conflicti521 – 5211A → G in CAA43209 (PubMed:1740329).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei660 – 769110VDPRP…LPSIL → GEAGARTGGVGRSGGKVAGL EGMQGQGFLGSGRVGWEATA DRKDKWLEGWTPGTKSHRNR IPRTRACEHGFQPGCSQRPL VRSSRVRIFGERDSETQV in isoform KV3.3A. CuratedVSP_001021Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69381 mRNA. Translation: AAC60679.1.
AC157653 Genomic DNA. No translation available.
X60796, X60797 Genomic DNA. Translation: CAA43209.1.
PIRiA42073.
UniGeneiMm.40312.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69381 mRNA. Translation: AAC60679.1.
AC157653 Genomic DNA. No translation available.
X60796, X60797 Genomic DNA. Translation: CAA43209.1.
PIRiA42073.
UniGeneiMm.40312.

3D structure databases

ProteinModelPortaliQ63959.
SMRiQ63959. Positions 90-194, 272-546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103540.

PTM databases

iPTMnetiQ63959.
PhosphoSiteiQ63959.

Proteomic databases

MaxQBiQ63959.
PaxDbiQ63959.
PeptideAtlasiQ63959.
PRIDEiQ63959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:96669. Kcnc3.

Phylogenomic databases

eggNOGiKOG3713. Eukaryota.
COG1226. LUCA.
HOGENOMiHOG000231012.
HOVERGENiHBG105862.
InParanoidiQ63959.

Miscellaneous databases

PROiQ63959.
SOURCEiSearch...

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003974. K_chnl_volt-dep_Kv3.
IPR005404. K_chnl_volt-dep_Kv3.3.
IPR021105. K_chnl_volt-dep_Kv3_ID.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 3 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11404. Potassium_chann. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01582. KV33CHANNEL.
PR01491. KVCHANNEL.
PR01498. SHAWCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Kv3.3b: a novel Shaw type potassium channel expressed in terminally differentiated cerebellar Purkinje cells and deep cerebellar nuclei."
    Goldman-Wohl D.S., Chan E., Baird D., Heintz N.
    J. Neurosci. 14:511-522(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM KV3.3B).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Genomic organization, nucleotide sequence, and cellular distribution of a Shaw-related potassium channel gene, Kv3.3, and mapping of Kv3.3 and Kv3.4 to human chromosomes 19 and 1."
    Ghanshani S., Pak M., McPherson J.D., Strong M., Dethlefs B., Wasmuth J.J., Salkoff L.A., Gutman G.A., Chandy G.K.
    Genomics 12:190-196(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 78-769 (ISOFORM KV3.3A).
    Strain: AKR/J.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-687; SER-692; SER-731; SER-734; SER-742; THR-750 AND SER-754, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiKCNC3_MOUSE
AccessioniPrimary (citable) accession number: Q63959
Secondary accession number(s): E9Q5E5, Q62088
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.