Q63959 (KCNC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily C member 3

Alternative name(s):
KSHIIID
Voltage-gated potassium channel subunit Kv3.3

Gene names

Name:

Kcnc3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	769 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Subunit structure	Heterotetramer of potassium channel proteins By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	The KV3.3B isoform is highly enriched in the brain, particularly in the cerebellum, where its expression is confined to Purkinje cells and deep cerebellar nuclei. Isoform KV3.3A is not expressed in cerebellum.
Developmental stage	Expression of KV3.3B begins in cerebellar Purkinje cells between postnatal day 8 (P8) and P10 and continues through adulthood.
Domain	The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
Sequence similarities	Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.3/KCNC3 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein homooligomerization Inferred from electronic annotation. Source: InterPro regulation of neurotransmitter secretion Inferred from mutant phenotype PubMed 15610163. Source: MGI
Cellular_component	axolemma Inferred from direct assay PubMed 18682278. Source: MGI axon terminus Inferred from direct assay PubMed 15610163. Source: MGI dendrite membrane Inferred from direct assay PubMed 18682278. Source: MGI neuromuscular junction Inferred from direct assay PubMed 15610163. Source: MGI neuronal cell body membrane Inferred from direct assay PubMed 18682278. Source: MGI voltage-gated potassium channel complex Inferred from electronic annotation. Source: InterPro
Molecular_function	delayed rectifier potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform KV3.3B (identifier: Q63959-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform KV3.3A (identifier: Q63959-2)

The sequence of this isoform differs from the canonical sequence as follows:
     660-769: VDPRPNGDPA...RAPPTLPSIL → GEAGARTGGV...FGERDSETQV

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 769	769	Potassium voltage-gated channel subfamily C member 3		PRO_0000054056
Regions
	Topological domain	1 – 290	290	Cytoplasmic Potential
	Transmembrane	291 – 309	19	Helical; Name=Segment S1; Potential
	Transmembrane	351 – 370	20	Helical; Name=Segment S2; Potential
	Topological domain	371 – 379	9	Cytoplasmic Potential
	Transmembrane	380 – 398	19	Helical; Name=Segment S3; Potential
	Transmembrane	412 – 434	23	Helical; Voltage-sensor; Name=Segment S4; Potential
	Topological domain	435 – 447	13	Cytoplasmic Potential
	Transmembrane	448 – 469	22	Helical; Name=Segment S5; Potential
	Transmembrane	518 – 539	22	Helical; Name=Segment S6; Potential
	Topological domain	540 – 769	230	Cytoplasmic Potential
	Motif	503 – 508	6	Selectivity filter By similarity
	Compositional bias	41 – 44	4	Poly-Gln
	Compositional bias	82 – 86	5	Poly-Gly
	Compositional bias	229 – 234	6	Poly-Gly
	Compositional bias	577 – 587	11	Poly-Pro
	Compositional bias	596 – 599	4	Poly-Pro
	Compositional bias	669 – 674	6	Poly-Ala
Amino acid modifications
	Glycosylation	320	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	483	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	660 – 769	110	VDPRP…LPSIL → GEAGARTGGVGRSGGKVAGL EGMQGQGFLGSGRVGWEATA DRKDKWLEGWTPGTKSHRNR IPRTRACEHGFQPGCSQRPL VRSSRVRIFGERDSETQV in isoform KV3.3A.		VSP_001021
Experimental info
	Sequence conflict	13	1	R → C in AAC60679. Ref.1
	Sequence conflict	51 – 52	2	AA → GP in AAC60679. Ref.1
	Sequence conflict	55	1	A → G in AAC60679. Ref.1
	Sequence conflict	256	1	G → D in CAA43209. Ref.3
	Sequence conflict	257 – 258	2	PA → LP in AAC60679. Ref.1
	Sequence conflict	260 – 266	7	GAGGAGG → ARAAGA in CAA43209. Ref.3
	Sequence conflict	521	1	A → G in AAC60679. Ref.1
	Sequence conflict	521	1	A → G in CAA43209. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform KV3.3B [UniParc]. Last modified July 27, 2011. Version 2. Checksum: C910236DE8AF04C9	FASTA	769	82,143
	10 20 30 40 50 60 MLSSVCVWSF RGRQGTGKQQ PQPVPTPQPP ESSPPPLPPP QQQQCSQPGT AASPAVPAFL 70 80 90 100 110 120 RPGGRRAEPC PGLPAVAMGR HGGGGGDSGK IVINVGGVRH ETYRSTLRTL PGTRLAGLTE 130 140 150 160 170 180 PEAAARFDYD PGTDEFFFDR HPGVFAYVLN YYRTGKLHCP ADVCGPLFEE ELGFWGIDET 190 200 210 220 230 240 DVEACCWMTY RQHRDAEEAL DSFEAPDSSA NANANAGGAH DAGLDDEAGA GGGGLDGAGG 250 260 270 280 290 300 ELKRLCFQDA GGGAGGPAGG AGGAGGTWWR RWQPRVWALF EDPYSSRAAR YVAFASLFFI 310 320 330 340 350 360 LISITTFCLE THEGFIHISN KTVTQASPIP GAPPENITNV EVETEPFLTY VEGVCVVWFT 370 380 390 400 410 420 FEFLMRVTFC PDKVEFLKSS LNIIDCVAIL PFYLEVGLSG LSSKAAKDVL GFLRVVRFVR 430 440 450 460 470 480 ILRIFKLTRH FVGLRVLGHT LRASTNEFLL LIIFLALGVL IFATMIYYAE RIGADPDDIL 490 500 510 520 530 540 GSNHTYFKNI PIGFWWAVVT MTTLGYGDMY PKTWSGMLVG ALCALAGVLT IAMPVPVIVN 550 560 570 580 590 600 NFGMYYSLAM AKQKLPKKKN KHIPRPPQPG SPNYCKPDPP PPPPPHPHHG SGGISPPPPI 610 620 630 640 650 660 TPPSMGVNVA GAYPPGPHTH PGLLRGGAGG LGIMGLPPLP APGEPCPLAQ EEVIETNRAV 670 680 690 700 710 720 DPRPNGDPAA AALAHEDCPA IDQPAMSPED KSPITPGSRG RYSRDRACFL VTDYAPSPDG 730 740 750 760 SIRKGYEKSR SLSSIVGLSG VSLRLAPLAT PPGSPRATRR APPTLPSIL
	Isoform KV3.3A [UniParc]. Checksum: E948AE4B942F23F6	FASTA	757	81,190
	10 20 30 40 50 60 MLSSVCVWSF RGRQGTGKQQ PQPVPTPQPP ESSPPPLPPP QQQQCSQPGT AASPAVPAFL 70 80 90 100 110 120 RPGGRRAEPC PGLPAVAMGR HGGGGGDSGK IVINVGGVRH ETYRSTLRTL PGTRLAGLTE 130 140 150 160 170 180 PEAAARFDYD PGTDEFFFDR HPGVFAYVLN YYRTGKLHCP ADVCGPLFEE ELGFWGIDET 190 200 210 220 230 240 DVEACCWMTY RQHRDAEEAL DSFEAPDSSA NANANAGGAH DAGLDDEAGA GGGGLDGAGG 250 260 270 280 290 300 ELKRLCFQDA GGGAGGPAGG AGGAGGTWWR RWQPRVWALF EDPYSSRAAR YVAFASLFFI 310 320 330 340 350 360 LISITTFCLE THEGFIHISN KTVTQASPIP GAPPENITNV EVETEPFLTY VEGVCVVWFT 370 380 390 400 410 420 FEFLMRVTFC PDKVEFLKSS LNIIDCVAIL PFYLEVGLSG LSSKAAKDVL GFLRVVRFVR 430 440 450 460 470 480 ILRIFKLTRH FVGLRVLGHT LRASTNEFLL LIIFLALGVL IFATMIYYAE RIGADPDDIL 490 500 510 520 530 540 GSNHTYFKNI PIGFWWAVVT MTTLGYGDMY PKTWSGMLVG ALCALAGVLT IAMPVPVIVN 550 560 570 580 590 600 NFGMYYSLAM AKQKLPKKKN KHIPRPPQPG SPNYCKPDPP PPPPPHPHHG SGGISPPPPI 610 620 630 640 650 660 TPPSMGVNVA GAYPPGPHTH PGLLRGGAGG LGIMGLPPLP APGEPCPLAQ EEVIETNRAG 670 680 690 700 710 720 EAGARTGGVG RSGGKVAGLE GMQGQGFLGS GRVGWEATAD RKDKWLEGWT PGTKSHRNRI 730 740 750 PRTRACEHGF QPGCSQRPLV RSSRVRIFGE RDSETQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Kv3.3b: a novel Shaw type potassium channel expressed in terminally differentiated cerebellar Purkinje cells and deep cerebellar nuclei." Goldman-Wohl D.S., Chan E., Baird D., Heintz N. J. Neurosci. 14:511-522(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM KV3.3B).
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Genomic organization, nucleotide sequence, and cellular distribution of a Shaw-related potassium channel gene, Kv3.3, and mapping of Kv3.3 and Kv3.4 to human chromosomes 19 and 1." Ghanshani S., Pak M., McPherson J.D., Strong M., Dethlefs B., Wasmuth J.J., Salkoff L.A., Gutman G.A., Chandy G.K. Genomics 12:190-196(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 78-769 (ISOFORM KV3.3A). Strain: AKR/J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S69381 mRNA. Translation: AAC60679.1. AC157653 Genomic DNA. No translation available. X60796, X60797 Genomic DNA. Translation: CAA43209.1.
IPI	IPI00229264. IPI00467430.
PIR	A42073.
UniGene	Mm.40312.
3D structure databases
ProteinModelPortal	Q63959.
SMR	Q63959. Positions 90-194, 272-546.
ModBase	Search...
PTM databases
PhosphoSite	Q63959.
Proteomic databases
PaxDb	Q63959.
PRIDE	Q63959.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:96669. Kcnc3.
Phylogenomic databases
eggNOG	NOG280627.
HOGENOM	HOG000231012.
HOVERGEN	HBG105862.
InParanoid	Q63959.
Gene expression databases
Genevestigator	Q63959.
GermOnline	ENSMUSG00000062785. Mus musculus.
Family and domain databases
Gene3D	3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR005821. Ion_trans_dom. IPR003091. K_chnl. IPR003968. K_chnl_volt-dep_Kv. IPR003974. K_chnl_volt-dep_Kv3. IPR005404. K_chnl_volt-dep_Kv3.3. IPR003131. T1-type_BTB. [Graphical view]
PANTHER	PTHR11537. PTHR11537. 1 hit.
Pfam	PF00520. Ion_trans. 1 hit. PF02214. K_tetra. 1 hit. [Graphical view]
PRINTS	PR00169. KCHANNEL. PR01582. KV33CHANNEL. PR01491. KVCHANNEL. PR01498. SHAWCHANNEL.
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

KCNC3_MOUSE

Accession

Primary (citable) accession number: Q63959
Secondary accession number(s): E9Q5E5, Q62088

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program