ID   PO4F3_MOUSE             Reviewed;         338 AA.
AC   Q63955; A6H6N6;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   24-JAN-2024, entry version 173.
DE   RecName: Full=POU domain, class 4, transcription factor 3 {ECO:0000312|MGI:MGI:102523};
DE   AltName: Full=Brain-specific homeobox/POU domain protein 3C;
DE            Short=Brain-3C;
DE            Short=Brn-3C;
DE   AltName: Full=Brn-3.1 {ECO:0000303|PubMed:8637595};
GN   Name=Pou4f3 {ECO:0000312|MGI:MGI:102523};
GN   Synonyms=Brn-3.1 {ECO:0000303|PubMed:8637595}, Brn-3c
GN   {ECO:0000312|MGI:MGI:102523}, Brn3c {ECO:0000312|MGI:MGI:102523};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CD;
RX   PubMed=8162704; DOI=10.1159/000133709;
RA   Theil T., Zechner U., Klett C., Adolph S., Moeroey T.;
RT   "Chromosomal localization and sequences of the murine Brn-3 family of
RT   developmental control genes.";
RL   Cytogenet. Cell Genet. 66:267-271(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Brain;
RX   PubMed=8248179; DOI=10.1073/pnas.90.22.10841;
RA   Gerrero M.R., McEvilly R.J., Turner E., Lin C.R., O'Connell S., Jenne K.J.,
RA   Hobbs M.V., Rosenfeld M.G.;
RT   "Brn-3.0: a POU-domain protein expressed in the sensory, immune, and
RT   endocrine systems that functions on elements distinct from known octamer
RT   motifs.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:10841-10845(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   FUNCTION, DNA-BINDING, AND DEVELOPMENTAL STAGE.
RX   PubMed=8290353; DOI=10.1093/nar/21.25.5921;
RA   Theil T., McLean-Hunter S., Zoernig M., Moeroey T.;
RT   "Mouse Brn-3 family of POU transcription factors: a new aminoterminal
RT   domain is crucial for the oncogenic activity of Brn-3a.";
RL   Nucleic Acids Res. 21:5921-5929(1993).
RN   [6]
RP   FUNCTION, AND DNA-BINDING.
RX   PubMed=7935408; DOI=10.1128/mcb.14.10.6907-6914.1994;
RA   Morris P.J., Theil T., Ring C.J., Lillycrop K.A., Moroy T., Latchman D.S.;
RT   "The opposite and antagonistic effects of the closely related POU family
RT   transcription factors Brn-3a and Brn-3b on the activity of a target
RT   promoter are dependent on differences in the POU domain.";
RL   Mol. Cell. Biol. 14:6907-6914(1994).
RN   [7]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=8637595; DOI=10.1038/381603a0;
RA   Erkman L., McEvilly R.J., Luo L., Ryan A.K., Hooshmand F., O'Connell S.M.,
RA   Keithley E.M., Rapaport D.H., Ryan A.F., Rosenfeld M.G.;
RT   "Role of transcription factors Brn-3.1 and Brn-3.2 in auditory and visual
RT   system development.";
RL   Nature 381:603-606(1996).
RN   [8]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=22326227; DOI=10.1016/j.ydbio.2012.01.021;
RA   Zou M., Li S., Klein W.H., Xiang M.;
RT   "Brn3a/Pou4f1 regulates dorsal root ganglion sensory neuron specification
RT   and axonal projection into the spinal cord.";
RL   Dev. Biol. 364:114-127(2012).
RN   [9]
RP   INTERACTION WITH ISL1.
RX   PubMed=24643061; DOI=10.1371/journal.pone.0092105;
RA   Li R., Wu F., Ruonala R., Sapkota D., Hu Z., Mu X.;
RT   "Isl1 and Pou4f2 form a complex to regulate target genes in developing
RT   retinal ganglion cells.";
RL   PLoS ONE 9:E92105-E92105(2014).
CC   -!- FUNCTION: Acts as a transcriptional activator (PubMed:8290353,
CC       PubMed:7935408). Acts by binding to sequences related to the consensus
CC       octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target
CC       genes (PubMed:7935408). Involved in the auditory system development,
CC       required for terminal differentiation of hair cells in the inner ear
CC       (PubMed:8637595). {ECO:0000269|PubMed:7935408,
CC       ECO:0000269|PubMed:8290353, ECO:0000269|PubMed:8637595}.
CC   -!- SUBUNIT: Interacts with ISL1 (PubMed:24643061).
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15319}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q15319}. Note=Preferentially localized in the
CC       nucleus. {ECO:0000250|UniProtKB:Q15319}.
CC   -!- TISSUE SPECIFICITY: Brain.
CC   -!- DEVELOPMENTAL STAGE: Expressed in developing spinal cord from 13 dpc to
CC       postanal day 1, not expressed in adults (PubMed:8290353). Expressed by
CC       few neurons of dorsal root ganglion from, at least, 10.5 dpc to 15.5
CC       dpc (PubMed:22326227). {ECO:0000269|PubMed:22326227,
CC       ECO:0000269|PubMed:8290353}.
CC   -!- DISRUPTION PHENOTYPE: Mutants are deaf and show deficits in balance and
CC       coordination that become severe at about P14 and exhibit hyperactivity
CC       by 5-6 weeks. {ECO:0000269|PubMed:8637595}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-4
CC       subfamily. {ECO:0000305}.
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DR   EMBL; S69352; AAB30579.1; -; Genomic_DNA.
DR   EMBL; CH466528; EDL10035.1; -; Genomic_DNA.
DR   EMBL; BC145941; AAI45942.1; -; mRNA.
DR   CCDS; CCDS29212.1; -.
DR   PIR; B49642; B49642.
DR   RefSeq; NP_620395.2; NM_138945.2.
DR   AlphaFoldDB; Q63955; -.
DR   SMR; Q63955; -.
DR   STRING; 10090.ENSMUSP00000025374; -.
DR   PhosphoSitePlus; Q63955; -.
DR   PaxDb; 10090-ENSMUSP00000025374; -.
DR   Antibodypedia; 15803; 339 antibodies from 31 providers.
DR   DNASU; 18998; -.
DR   Ensembl; ENSMUST00000025374.4; ENSMUSP00000025374.4; ENSMUSG00000024497.5.
DR   GeneID; 18998; -.
DR   KEGG; mmu:18998; -.
DR   UCSC; uc008etv.2; mouse.
DR   AGR; MGI:102523; -.
DR   CTD; 5459; -.
DR   MGI; MGI:102523; Pou4f3.
DR   VEuPathDB; HostDB:ENSMUSG00000024497; -.
DR   eggNOG; KOG1168; Eukaryota.
DR   GeneTree; ENSGT00940000160880; -.
DR   HOGENOM; CLU_013065_0_0_1; -.
DR   InParanoid; Q63955; -.
DR   OMA; MMAMNTK; -.
DR   OrthoDB; 4250502at2759; -.
DR   PhylomeDB; Q63955; -.
DR   TreeFam; TF316413; -.
DR   BioGRID-ORCS; 18998; 1 hit in 78 CRISPR screens.
DR   PRO; PR:Q63955; -.
DR   Proteomes; UP000000589; Chromosome 18.
DR   RNAct; Q63955; Protein.
DR   Bgee; ENSMUSG00000024497; Expressed in lumbar dorsal root ganglion and 27 other cell types or tissues.
DR   ExpressionAtlas; Q63955; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0005667; C:transcription regulator complex; IC:MGI.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0048675; P:axon extension; IGI:MGI.
DR   GO; GO:0042491; P:inner ear auditory receptor cell differentiation; IMP:MGI.
DR   GO; GO:0048839; P:inner ear development; IMP:MGI.
DR   GO; GO:0042472; P:inner ear morphogenesis; IMP:MGI.
DR   GO; GO:0060113; P:inner ear receptor cell differentiation; IMP:MGI.
DR   GO; GO:0050885; P:neuromuscular process controlling balance; IMP:MGI.
DR   GO; GO:0051402; P:neuron apoptotic process; IMP:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:MGI.
DR   GO; GO:0031290; P:retinal ganglion cell axon guidance; IGI:MGI.
DR   GO; GO:0007605; P:sensory perception of sound; IMP:MGI.
DR   GO; GO:0021562; P:vestibulocochlear nerve development; IMP:MGI.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   PANTHER; PTHR11636; POU DOMAIN; 1.
DR   PANTHER; PTHR11636:SF43; POU DOMAIN, CLASS 4, TRANSCRIPTION FACTOR 3; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
DR   Genevisible; Q63955; MM.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; Differentiation; DNA-binding; Homeobox; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..338
FT                   /note="POU domain, class 4, transcription factor 3"
FT                   /id="PRO_0000100743"
FT   DOMAIN          179..256
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        274..333
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   MOTIF           56..65
FT                   /note="POU-IV box"
FT   CONFLICT        8..9
FT                   /note="QP -> HR (in Ref. 1; AAB30579)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        55
FT                   /note="A -> E (in Ref. 2; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        158
FT                   /note="A -> P (in Ref. 2; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        210
FT                   /note="A -> S (in Ref. 2; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        338
FT                   /note="H -> D (in Ref. 1; AAB30579)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   338 AA;  37016 MW;  4B7C78BD788FB944 CRC64;
     MMAMNAKQPF GMHPVLQEPK FSSLHSGSEA MRRVCLPAPQ LQGNIFGSFD ESLLARAEAL
     AAVDIVSHGK NHPFKPDATY HTMSSVPCTS TSPTVPISHP AALTSHPHHA VHQGLEGDLL
     EHISPTLSVS GLGAPEHSVM PAQIHPHHLG AMGHLHQAMG MSHPHAVAPH SAMPACLSDV
     ESDPRELEAF AERFKQRRIK LGVTQADVGA ALANLKIPGV GSLSQSTICR FESLTLSHNN
     MIALKPVLQA WLEEAEAAYR EKNSKPELFN GSERKRKRTS IAAPEKRSLE AYFAIQPRPS
     SEKIAAIAEK LDLKKNVVRV WFCNQRQKQK RMKYSAVH
//