Q63934 (PO4F2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
POU domain, class 4, transcription factor 2

Alternative name(s):
Brain-specific homeobox/POU domain protein 3B
Short name=Brain-3B
Short name=Brn-3B
Brn-3.2

Gene names

Name:	Pou4f2
Synonyms:	Brn3b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Ref.1
Subcellular location	Nucleus speckle By similarity.
Tissue specificity	Brain, peripheral sensory nervous system and retina. In the adult nervous system BRN-3.2 predominates in the optical, intermediate, and deep gray areas of the superior colliculus, the dorsal column of the mesencephalic and pontine central gray, and the lateral interpeduncular nucleus. Ref.1
Developmental stage	In the CNS it is selectively expressed in postmitotic, terminally differentiated neurons.
Sequence similarities	Belongs to the POU transcription factor family. Class-4 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	MAPKKK cascade Inferred from sequence or structural similarity. Source: UniProtKB axon extension involved in development Inferred from genetic interaction. Source: MGI intracellular estrogen receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB neuromuscular process controlling balance Inferred from genetic interaction. Source: MGI positive regulation of cell differentiation Inferred from mutant phenotype. Source: MGI retina development in camera-type eye Inferred from mutant phenotype. Source: MGI retinal ganglion cell axon guidance Inferred from mutant phenotype. Source: MGI sensory perception of sound Inferred from genetic interaction. Source: MGI
Cellular component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Inferred from direct assay. Source: UniProtKB RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay. Source: UniProtKB chromatin binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	POU domain, class 4, transcription factor 2		PRO_0000100741
Regions
	Domain	252 – 329	78	POU-specific
	DNA binding	347 – 406	60	Homeobox
	Motif	112 – 121	10	POU-IV box
	Compositional bias	1 – 4	4	Poly-Met
	Compositional bias	43 – 51	9	Poly-Ser
	Compositional bias	54 – 67	14	Poly-Gly
	Compositional bias	69 – 75	7	Poly-Ser
	Compositional bias	76 – 85	10	Poly-Gly
	Compositional bias	128 – 131	4	Poly-His
	Compositional bias	157 – 160	4	Poly-Ser
	Compositional bias	173 – 184	12	Poly-His
Experimental info
	Sequence conflict	90 – 98	9	RRACLPTPP → MCAFYLQLQ Ref.3
	Sequence conflict	404 – 405	2	RM → KV in AAB30578. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q63934 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 97EA816F7DEC07C9	FASTA	411	43,173
	10 20 30 40 50 60 MMMMSLNSKQ AFSMPHAGSL HVEPKYSALH SASPGSSAPA APSASSPSSS SNAGGGGGGG 70 80 90 100 110 120 GGGGGGGRSS SSSSSGSGGS GGGGGSEAMR RACLPTPPSN IFGGLDESLL ARAEALAAVD 130 140 150 160 170 180 IVSQSKSHHH HPPHHSPFKP DATYHTMNTI PCTSAASSSS VPISHPSALA GTHHHHHHHH 190 200 210 220 230 240 HHHHQPHQAL EGELLEHLSP GLALGAMAGP DGTVVSTPAH APHMATMNPM HQAALSMAHA 250 260 270 280 290 300 HGLPSHMGCM SDVDADPRDL EAFAERFKQR RIKLGVTQAD VGSALANLKI PGVGSLSQST 310 320 330 340 350 360 ICRFESLTLS HNNMIALKPI LQAWLEEAEK SHREKLTKPE LFNGAEKKRK RTSIAAPEKR 370 380 390 400 410 SLEAYFAIQP RPSSEKIAAI AEKLDLKKNV VRVWFCNQRQ KQKRMKYSAG I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Brn-3.2: a Brn-3-related transcription factor with distinctive central nervous system expression and regulation by retinoic acid." Turner E.E., Jenne K.J., Rosenfeld M.G. Neuron 12:205-218(1994) [PubMed: 7904822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Eye.
[3]	"Chromosomal localization and sequences of the murine Brn-3 family of developmental control genes." Theil T., Zechner U., Klett C., Adolph S., Moeroey T. Cytogenet. Cell Genet. 66:267-271(1994) [PubMed: 8162704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 90-411. Strain: CD.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S68377 mRNA. Translation: AAC60672.1. AK087546 mRNA. Translation: BAC39921.1. S69351 Genomic DNA. Translation: AAB30578.1.
IPI	IPI00135712.
RefSeq	NP_620394.2. NM_138944.2.
UniGene	Mm.234261.
3D structure databases
ProteinModelPortal	Q63934.
SMR	Q63934. Positions 255-407.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63934.
Proteomic databases
PRIDE	Q63934.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034115; ENSMUSP00000034115; ENSMUSG00000031688.
GeneID	18997.
KEGG	mmu:18997.
UCSC	uc009mhy.1. mouse.
Organism-specific databases
CTD	5458.
MGI	MGI:102524. Pou4f2.
Phylogenomic databases
GeneTree	ENSGT00580000081294.
HOGENOM	HBG714425.
HOVERGEN	HBG031829.
InParanoid	Q63934.
OMA	MMSLNSK.
OrthoDB	EOG42RD7X.
PhylomeDB	Q63934.
Gene expression databases
ArrayExpress	Q63934.
Bgee	Q63934.
CleanEx	MM_POU4F2.
Genevestigator	Q63934.
GermOnline	ENSMUSG00000031688. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR010982. Lambda_DNA-bd. IPR013847. POU. IPR015584. POU4-related. IPR000327. POU_specific. [Graphical view]
Gene3D	G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit. G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K09366.
PANTHER	PTHR11636:SF10. POU_4_related. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view]
PRINTS	PR00028. POUDOMAIN.
SMART	SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF47413. Lambda_like_DNA. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	295402.
SOURCE	Search...

Entry information

Entry name

PO4F2_MOUSE

Accession

Primary (citable) accession number: Q63934
Secondary accession number(s): Q63954

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program