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Protein

POU domain, class 4, transcription factor 2

Gene

Pou4f2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi347 – 40660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • axon extension Source: MGI
  • axon guidance Source: MGI
  • axonogenesis Source: MGI
  • intracellular estrogen receptor signaling pathway Source: UniProtKB
  • MAPK cascade Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • neuromuscular process controlling balance Source: MGI
  • neuron differentiation Source: MGI
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • retina development in camera-type eye Source: MGI
  • retinal ganglion cell axon guidance Source: MGI
  • sensory perception of sound Source: MGI
  • spermatogenesis Source: Ensembl
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 4, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 3B
Short name:
Brain-3B
Short name:
Brn-3B
Brn-3.2
Gene namesi
Name:Pou4f2
Synonyms:Brn3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:102524. Pou4f2.

Subcellular locationi

GO - Cellular componenti

  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411POU domain, class 4, transcription factor 2PRO_0000100741Add
BLAST

Proteomic databases

PaxDbiQ63934.
PRIDEiQ63934.

PTM databases

PhosphoSiteiQ63934.

Expressioni

Tissue specificityi

Brain, peripheral sensory nervous system and retina. In the adult nervous system BRN-3.2 predominates in the optical, intermediate, and deep gray areas of the superior colliculus, the dorsal column of the mesencephalic and pontine central gray, and the lateral interpeduncular nucleus.1 Publication

Developmental stagei

In the CNS it is selectively expressed in postmitotic, terminally differentiated neurons.

Gene expression databases

BgeeiQ63934.
CleanExiMM_POU4F2.
GenevisibleiQ63934. MM.

Interactioni

Protein-protein interaction databases

BioGridi202311. 4 interactions.
STRINGi10090.ENSMUSP00000034115.

Structurei

3D structure databases

ProteinModelPortaliQ63934.
SMRiQ63934. Positions 256-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini252 – 32978POU-specificPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi112 – 12110POU-IV box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 44Poly-Met
Compositional biasi43 – 519Poly-Ser
Compositional biasi54 – 6714Poly-GlyAdd
BLAST
Compositional biasi69 – 757Poly-Ser
Compositional biasi76 – 8510Poly-Gly
Compositional biasi128 – 1314Poly-His
Compositional biasi157 – 1604Poly-Ser
Compositional biasi173 – 18412Poly-HisAdd
BLAST

Domaini

The polyhistidine repeat acts as a targeting signal to nuclear speckles.By similarity

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG289030.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116305.
HOVERGENiHBG031829.
InParanoidiQ63934.
KOiK09366.
OMAiTHAPHMA.
OrthoDBiEOG7RV9G7.
PhylomeDBiQ63934.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMMMSLNSKQ AFSMPHAGSL HVEPKYSALH SASPGSSAPA APSASSPSSS
60 70 80 90 100
SNAGGGGGGG GGGGGGGRSS SSSSSGSGGS GGGGGSEAMR RACLPTPPSN
110 120 130 140 150
IFGGLDESLL ARAEALAAVD IVSQSKSHHH HPPHHSPFKP DATYHTMNTI
160 170 180 190 200
PCTSAASSSS VPISHPSALA GTHHHHHHHH HHHHQPHQAL EGELLEHLSP
210 220 230 240 250
GLALGAMAGP DGTVVSTPAH APHMATMNPM HQAALSMAHA HGLPSHMGCM
260 270 280 290 300
SDVDADPRDL EAFAERFKQR RIKLGVTQAD VGSALANLKI PGVGSLSQST
310 320 330 340 350
ICRFESLTLS HNNMIALKPI LQAWLEEAEK SHREKLTKPE LFNGAEKKRK
360 370 380 390 400
RTSIAAPEKR SLEAYFAIQP RPSSEKIAAI AEKLDLKKNV VRVWFCNQRQ
410
KQKRMKYSAG I
Length:411
Mass (Da):43,173
Last modified:November 1, 1996 - v1
Checksum:i97EA816F7DEC07C9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 989RRACLPTPP → MCAFYLQLQ (PubMed:8162704).Curated
Sequence conflicti404 – 4052RM → KV in AAB30578 (PubMed:8162704).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68377 mRNA. Translation: AAC60672.1.
AK087546 mRNA. Translation: BAC39921.1.
S69351 Genomic DNA. Translation: AAB30578.1.
CCDSiCCDS22429.1.
RefSeqiNP_620394.2. NM_138944.2.
UniGeneiMm.234261.

Genome annotation databases

EnsembliENSMUST00000034115; ENSMUSP00000034115; ENSMUSG00000031688.
GeneIDi18997.
KEGGimmu:18997.
UCSCiuc009mhy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68377 mRNA. Translation: AAC60672.1.
AK087546 mRNA. Translation: BAC39921.1.
S69351 Genomic DNA. Translation: AAB30578.1.
CCDSiCCDS22429.1.
RefSeqiNP_620394.2. NM_138944.2.
UniGeneiMm.234261.

3D structure databases

ProteinModelPortaliQ63934.
SMRiQ63934. Positions 256-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202311. 4 interactions.
STRINGi10090.ENSMUSP00000034115.

PTM databases

PhosphoSiteiQ63934.

Proteomic databases

PaxDbiQ63934.
PRIDEiQ63934.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034115; ENSMUSP00000034115; ENSMUSG00000031688.
GeneIDi18997.
KEGGimmu:18997.
UCSCiuc009mhy.1. mouse.

Organism-specific databases

CTDi5458.
MGIiMGI:102524. Pou4f2.

Phylogenomic databases

eggNOGiNOG289030.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116305.
HOVERGENiHBG031829.
InParanoidiQ63934.
KOiK09366.
OMAiTHAPHMA.
OrthoDBiEOG7RV9G7.
PhylomeDBiQ63934.
TreeFamiTF316413.

Miscellaneous databases

NextBioi295402.
PROiQ63934.
SOURCEiSearch...

Gene expression databases

BgeeiQ63934.
CleanExiMM_POU4F2.
GenevisibleiQ63934. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Brn-3.2: a Brn-3-related transcription factor with distinctive central nervous system expression and regulation by retinoic acid."
    Turner E.E., Jenne K.J., Rosenfeld M.G.
    Neuron 12:205-218(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  3. "Chromosomal localization and sequences of the murine Brn-3 family of developmental control genes."
    Theil T., Zechner U., Klett C., Adolph S., Moeroey T.
    Cytogenet. Cell Genet. 66:267-271(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 90-411.
    Strain: CD.

Entry informationi

Entry nameiPO4F2_MOUSE
AccessioniPrimary (citable) accession number: Q63934
Secondary accession number(s): Q63954
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.