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Q63934 (PO4F2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 4, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 3B
Short name=Brain-3B
Short name=Brn-3B
Brn-3.2
Gene names
Name:Pou4f2
Synonyms:Brn3b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Ref.1

Subcellular location

Nucleus speckle By similarity.

Tissue specificity

Brain, peripheral sensory nervous system and retina. In the adult nervous system BRN-3.2 predominates in the optical, intermediate, and deep gray areas of the superior colliculus, the dorsal column of the mesencephalic and pontine central gray, and the lateral interpeduncular nucleus. Ref.1

Developmental stage

In the CNS it is selectively expressed in postmitotic, terminally differentiated neurons.

Domain

The polyhistidine repeat acts as a targeting signal to nuclear speckles By similarity.

Sequence similarities

Belongs to the POU transcription factor family. Class-4 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processMAPK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

axon extension

Inferred from genetic interaction PubMed 11807038. Source: MGI

intracellular estrogen receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

neuromuscular process controlling balance

Inferred from genetic interaction PubMed 11807038. Source: MGI

positive regulation of cell differentiation

Inferred from mutant phenotype PubMed 18367606. Source: MGI

retina development in camera-type eye

Inferred from mutant phenotype PubMed 8637595PubMed 9630743. Source: MGI

retinal ganglion cell axon guidance

Inferred from mutant phenotype PubMed 18434421. Source: MGI

sensory perception of sound

Inferred from genetic interaction PubMed 11807038. Source: MGI

   Cellular_componentnuclear speck

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay PubMed 12609742PubMed 7623109. Source: UniProtKB

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription

Inferred from direct assay PubMed 8670054. Source: UniProtKB

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 18368538. Source: UniProtKB

chromatin binding

Inferred from direct assay PubMed 18434421. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411POU domain, class 4, transcription factor 2
PRO_0000100741

Regions

Domain252 – 32978POU-specific
DNA binding347 – 40660Homeobox
Motif112 – 12110POU-IV box
Compositional bias1 – 44Poly-Met
Compositional bias43 – 519Poly-Ser
Compositional bias54 – 6714Poly-Gly
Compositional bias69 – 757Poly-Ser
Compositional bias76 – 8510Poly-Gly
Compositional bias128 – 1314Poly-His
Compositional bias157 – 1604Poly-Ser
Compositional bias173 – 18412Poly-His

Experimental info

Sequence conflict90 – 989RRACLPTPP → MCAFYLQLQ Ref.3
Sequence conflict404 – 4052RM → KV in AAB30578. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q63934 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 97EA816F7DEC07C9

FASTA41143,173
        10         20         30         40         50         60 
MMMMSLNSKQ AFSMPHAGSL HVEPKYSALH SASPGSSAPA APSASSPSSS SNAGGGGGGG 

        70         80         90        100        110        120 
GGGGGGGRSS SSSSSGSGGS GGGGGSEAMR RACLPTPPSN IFGGLDESLL ARAEALAAVD 

       130        140        150        160        170        180 
IVSQSKSHHH HPPHHSPFKP DATYHTMNTI PCTSAASSSS VPISHPSALA GTHHHHHHHH 

       190        200        210        220        230        240 
HHHHQPHQAL EGELLEHLSP GLALGAMAGP DGTVVSTPAH APHMATMNPM HQAALSMAHA 

       250        260        270        280        290        300 
HGLPSHMGCM SDVDADPRDL EAFAERFKQR RIKLGVTQAD VGSALANLKI PGVGSLSQST 

       310        320        330        340        350        360 
ICRFESLTLS HNNMIALKPI LQAWLEEAEK SHREKLTKPE LFNGAEKKRK RTSIAAPEKR 

       370        380        390        400        410 
SLEAYFAIQP RPSSEKIAAI AEKLDLKKNV VRVWFCNQRQ KQKRMKYSAG I 

« Hide

References

« Hide 'large scale' references
[1]"Brn-3.2: a Brn-3-related transcription factor with distinctive central nervous system expression and regulation by retinoic acid."
Turner E.E., Jenne K.J., Rosenfeld M.G.
Neuron 12:205-218(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Eye.
[3]"Chromosomal localization and sequences of the murine Brn-3 family of developmental control genes."
Theil T., Zechner U., Klett C., Adolph S., Moeroey T.
Cytogenet. Cell Genet. 66:267-271(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 90-411.
Strain: CD.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S68377 mRNA. Translation: AAC60672.1.
AK087546 mRNA. Translation: BAC39921.1.
S69351 Genomic DNA. Translation: AAB30578.1.
RefSeqNP_620394.2. NM_138944.2.
UniGeneMm.234261.

3D structure databases

ProteinModelPortalQ63934.
SMRQ63934. Positions 256-405.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202311. 4 interactions.
STRING10090.ENSMUSP00000034115.

PTM databases

PhosphoSiteQ63934.

Proteomic databases

PaxDbQ63934.
PRIDEQ63934.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034115; ENSMUSP00000034115; ENSMUSG00000031688.
GeneID18997.
KEGGmmu:18997.
UCSCuc009mhy.1. mouse.

Organism-specific databases

CTD5458.
MGIMGI:102524. Pou4f2.

Phylogenomic databases

eggNOGNOG289030.
GeneTreeENSGT00730000110439.
HOGENOMHOG000116305.
HOVERGENHBG031829.
InParanoidQ63934.
KOK09366.
OMAPSNIFGG.
OrthoDBEOG7RV9G7.
TreeFamTF316413.

Gene expression databases

BgeeQ63934.
CleanExMM_POU4F2.
GenevestigatorQ63934.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295402.
PROQ63934.
SOURCESearch...

Entry information

Entry namePO4F2_MOUSE
AccessionPrimary (citable) accession number: Q63934
Secondary accession number(s): Q63954
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot