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Protein

POU domain, class 4, transcription factor 2

Gene

Pou4f2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor (PubMed:8290353). May play a role in determining or maintaining the identities of a small subset of visual system neurons (PubMed:7904822).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi347 – 406HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • axon extension Source: MGI
  • axon guidance Source: MGI
  • axonogenesis Source: MGI
  • intracellular estrogen receptor signaling pathway Source: UniProtKB
  • MAPK cascade Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • neuromuscular process controlling balance Source: MGI
  • neuron differentiation Source: MGI
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • retina development in camera-type eye Source: MGI
  • retinal ganglion cell axon guidance Source: MGI
  • sensory perception of sound Source: MGI
  • spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6804759. Regulation of TP53 Activity through Association with Co-factors.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 4, transcription factor 2Imported
Alternative name(s):
Brain-specific homeobox/POU domain protein 3B
Short name:
Brain-3B
Short name:
Brn-3B
Brn-3.21 Publication
Gene namesi
Name:Pou4f2Imported
Synonyms:Brn-3.21 Publication, Brn3bImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:102524. Pou4f2.

Subcellular locationi

GO - Cellular componenti

  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

In mutants, mature retinal whole mounts show a loss of approximately 60-70% of retinal ganglion cells. Mutants develop to adulthood and are fertile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007411 – 411POU domain, class 4, transcription factor 2Add BLAST411

Proteomic databases

PaxDbiQ63934.
PRIDEiQ63934.

PTM databases

iPTMnetiQ63934.
PhosphoSitePlusiQ63934.

Expressioni

Tissue specificityi

Brain, peripheral sensory nervous system and retina. In the adult nervous system BRN-3.2 predominates in the optical, intermediate, and deep gray areas of the superior colliculus, the dorsal column of the mesencephalic and pontine central gray, and the lateral interpeduncular nucleus.1 Publication

Developmental stagei

In the central nervous system, selectively expressed in postmitotic, terminally differentiated neurons (PubMed:7904822). Expressed in developing spinal cord from E13 to postanal day 1, not expressed in adults (PubMed:8290353). At E10.5, weakly expressed in the dorsal root ganglion neurons, the expression increases at least to E15.5 (PubMed:22326227). At E13.5, expressed in retinal ganglion cells and peaks at E15.5 to decline late in development (PubMed:8637595).4 Publications

Gene expression databases

BgeeiENSMUSG00000031688.
CleanExiMM_POU4F2.
GenevisibleiQ63934. MM.

Interactioni

Protein-protein interaction databases

BioGridi202311. 4 interactors.
STRINGi10090.ENSMUSP00000034115.

Structurei

3D structure databases

ProteinModelPortaliQ63934.
SMRiQ63934.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini252 – 329POU-specificPROSITE-ProRule annotationAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi112 – 121POU-IV box10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 4Poly-Met4
Compositional biasi43 – 51Poly-Ser9
Compositional biasi54 – 67Poly-GlyAdd BLAST14
Compositional biasi69 – 75Poly-Ser7
Compositional biasi76 – 85Poly-Gly10
Compositional biasi128 – 131Poly-His4
Compositional biasi157 – 160Poly-Ser4
Compositional biasi173 – 184Poly-HisAdd BLAST12

Domaini

The polyhistidine repeat acts as a targeting signal to nuclear speckles.By similarity

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG1168. Eukaryota.
ENOG410XPNX. LUCA.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116305.
HOVERGENiHBG031829.
InParanoidiQ63934.
KOiK09366.
OMAiTHAPHMA.
OrthoDBiEOG091G0WBK.
PhylomeDBiQ63934.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMMMSLNSKQ AFSMPHAGSL HVEPKYSALH SASPGSSAPA APSASSPSSS
60 70 80 90 100
SNAGGGGGGG GGGGGGGRSS SSSSSGSGGS GGGGGSEAMR RACLPTPPSN
110 120 130 140 150
IFGGLDESLL ARAEALAAVD IVSQSKSHHH HPPHHSPFKP DATYHTMNTI
160 170 180 190 200
PCTSAASSSS VPISHPSALA GTHHHHHHHH HHHHQPHQAL EGELLEHLSP
210 220 230 240 250
GLALGAMAGP DGTVVSTPAH APHMATMNPM HQAALSMAHA HGLPSHMGCM
260 270 280 290 300
SDVDADPRDL EAFAERFKQR RIKLGVTQAD VGSALANLKI PGVGSLSQST
310 320 330 340 350
ICRFESLTLS HNNMIALKPI LQAWLEEAEK SHREKLTKPE LFNGAEKKRK
360 370 380 390 400
RTSIAAPEKR SLEAYFAIQP RPSSEKIAAI AEKLDLKKNV VRVWFCNQRQ
410
KQKRMKYSAG I
Length:411
Mass (Da):43,173
Last modified:November 1, 1996 - v1
Checksum:i97EA816F7DEC07C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90 – 98RRACLPTPP → MCAFYLQLQ in AAB30578 (PubMed:8162704).Curated9
Sequence conflicti404 – 405RM → KV in AAB30578 (PubMed:8162704).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68377 mRNA. Translation: AAC60672.1.
AK087546 mRNA. Translation: BAC39921.1.
S69351 Genomic DNA. Translation: AAB30578.1.
CCDSiCCDS22429.1.
RefSeqiNP_620394.2. NM_138944.2.
UniGeneiMm.234261.

Genome annotation databases

EnsembliENSMUST00000034115; ENSMUSP00000034115; ENSMUSG00000031688.
GeneIDi18997.
KEGGimmu:18997.
UCSCiuc009mhy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68377 mRNA. Translation: AAC60672.1.
AK087546 mRNA. Translation: BAC39921.1.
S69351 Genomic DNA. Translation: AAB30578.1.
CCDSiCCDS22429.1.
RefSeqiNP_620394.2. NM_138944.2.
UniGeneiMm.234261.

3D structure databases

ProteinModelPortaliQ63934.
SMRiQ63934.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202311. 4 interactors.
STRINGi10090.ENSMUSP00000034115.

PTM databases

iPTMnetiQ63934.
PhosphoSitePlusiQ63934.

Proteomic databases

PaxDbiQ63934.
PRIDEiQ63934.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034115; ENSMUSP00000034115; ENSMUSG00000031688.
GeneIDi18997.
KEGGimmu:18997.
UCSCiuc009mhy.1. mouse.

Organism-specific databases

CTDi5458.
MGIiMGI:102524. Pou4f2.

Phylogenomic databases

eggNOGiKOG1168. Eukaryota.
ENOG410XPNX. LUCA.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116305.
HOVERGENiHBG031829.
InParanoidiQ63934.
KOiK09366.
OMAiTHAPHMA.
OrthoDBiEOG091G0WBK.
PhylomeDBiQ63934.
TreeFamiTF316413.

Enzyme and pathway databases

ReactomeiR-MMU-6804759. Regulation of TP53 Activity through Association with Co-factors.

Miscellaneous databases

PROiQ63934.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031688.
CleanExiMM_POU4F2.
GenevisibleiQ63934. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPO4F2_MOUSE
AccessioniPrimary (citable) accession number: Q63934
Secondary accession number(s): Q63954
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.