Reviewed, UniProtKB/Swiss-Prot Q63934 (PO4F2_MOUSE)

Names and origin

Protein names

Recommended name:
    POU domain, class 4, transcription factor 2
Alternative name(s):
    Brain-specific homeobox/POU domain protein 3B
      Short name=Brn-3B
    Brn-3.2

Gene names

Name:	Pou4f2
Synonyms:	Brn3b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Subcellular location	Nucleus.
Tissue specificity	Brain, peripheral sensory nervous system and retina. In the adult nervous system BRN-3.2 predominates in the optical, intermediate, and deep gray areas of the superior colliculus, the dorsal column of the mesencephalic and pontine central gray, and the lateral interpeduncular nucleus.
Developmental stage	In the CNS it is selectively expressed in postmitotic, terminally differentiated neurons.
Sequence similarities	Belongs to the POU transcription factor family. Class-4 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Gene Ontology (GO)
Biological process	axon extension involved in development Inferred from genetic interaction. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: MGI neuromuscular process controlling balance Inferred from genetic interaction. Source: MGI positive regulation of cell differentiation Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI retina development in camera-type eye Inferred from mutant phenotype. Source: MGI retinal ganglion cell axon guidance Inferred from mutant phenotype. Source: MGI sensory perception of sound Inferred from genetic interaction. Source: MGI
Cellular component	nucleus Inferred from direct assay. Source: MGI
Molecular function	chromatin binding Inferred from direct assay. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	POU domain, class 4, transcription factor 2		PRO_0000100741
Regions
	Domain	252 – 329	78	POU-specific
	DNA binding	347 – 406	60	Homeobox
	Motif	112 – 121	10	POU-IV box
	Compositional bias	1 – 4	4	Poly-Met
	Compositional bias	43 – 51	9	Poly-Ser
	Compositional bias	54 – 67	14	Poly-Gly
	Compositional bias	69 – 75	7	Poly-Ser
	Compositional bias	76 – 85	10	Poly-Gly
	Compositional bias	128 – 131	4	Poly-His
	Compositional bias	157 – 160	4	Poly-Ser
	Compositional bias	173 – 184	12	Poly-His
Experimental info
	Sequence conflict	90 – 98	9	RRACLPTPP → MCAFYLQLQ Ref.3
	Sequence conflict	404 – 405	2	RM → KV in AAB30578. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q63934-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 97EA816F7DEC07C9	FASTA	411	43,173
	10 20 30 40 50 60 MMMMSLNSKQ AFSMPHAGSL HVEPKYSALH SASPGSSAPA APSASSPSSS SNAGGGGGGG 70 80 90 100 110 120 GGGGGGGRSS SSSSSGSGGS GGGGGSEAMR RACLPTPPSN IFGGLDESLL ARAEALAAVD 130 140 150 160 170 180 IVSQSKSHHH HPPHHSPFKP DATYHTMNTI PCTSAASSSS VPISHPSALA GTHHHHHHHH 190 200 210 220 230 240 HHHHQPHQAL EGELLEHLSP GLALGAMAGP DGTVVSTPAH APHMATMNPM HQAALSMAHA 250 260 270 280 290 300 HGLPSHMGCM SDVDADPRDL EAFAERFKQR RIKLGVTQAD VGSALANLKI PGVGSLSQST 310 320 330 340 350 360 ICRFESLTLS HNNMIALKPI LQAWLEEAEK SHREKLTKPE LFNGAEKKRK RTSIAAPEKR 370 380 390 400 410 SLEAYFAIQP RPSSEKIAAI AEKLDLKKNV VRVWFCNQRQ KQKRMKYSAG I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Brn-3.2: a Brn-3-related transcription factor with distinctive central nervous system expression and regulation by retinoic acid." Turner E.E., Jenne K.J., Rosenfeld M.G. Neuron 12:205-218(1994) [PubMed: 7904822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Eye.
[3]	"Chromosomal localization and sequences of the murine Brn-3 family of developmental control genes." Theil T., Zechner U., Klett C., Adolph S., Moeroey T. Cytogenet. Cell Genet. 66:267-271(1994) [PubMed: 8162704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 90-411. Strain: CD.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S68377 mRNA. Translation: AAC60672.1. AK087546 mRNA. Translation: BAC39921.1. S69351 Genomic DNA. Translation: AAB30578.1.
IPI	IPI00135712.
RefSeq	NP_620394.2.
UniGene	Mm.234261
3D structure databases
HSSP	HSSP built from PDB template 1CQT based on UniProtKB P14859.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000031688. Mus musculus. [Contig view]
GeneID	18997.
KEGG	mmu:18997.
Organism-specific databases
MGI	MGI:102524. Pou4f2.
Phylogenomic databases
HOGENOM	Q63934.
HOVERGEN	Q63934.
OMA	Q63934. MMMSLNS.
Gene expression databases
ArrayExpress	Q63934.
Bgee	Q63934.
CleanEx	MM_POU4F2.
GermOnline	ENSMUSG00000031688. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR012287. Homeodomain-rel. IPR013847. POU. IPR015584. POU_4_related. IPR000327. POU_specific. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
PANTHER	PTHR11636:SF10. POU_4_related. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view]
PRINTS	PR00028. POUDOMAIN.
ProDom	PD000010. Homeobox. 1 hit. PD000583. POU. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	295402.
SOURCE	Search...

Entry information

Entry name

PO4F2_MOUSE

Accession

Primary (citable) accession number: Q63934
Secondary accession number(s): Q63954

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 5, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)