Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q63881

- KCND2_RAT

UniProt

Q63881 - KCND2_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Potassium voltage-gated channel subfamily D member 2

Gene

Kcnd2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi105 – 1051ZincBy similarity
Metal bindingi132 – 1321ZincBy similarity
Metal bindingi133 – 1331ZincBy similarity

GO - Molecular functioni

  1. A-type (transient outward) potassium channel activity Source: RGD
  2. ion channel activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW
  4. potassium channel activity Source: RGD
  5. protein heterodimerization activity Source: RGD
  6. voltage-gated potassium channel activity Source: RGD

GO - Biological processi

  1. action potential Source: RGD
  2. cellular response to drug Source: RGD
  3. cellular response to mechanical stimulus Source: RGD
  4. potassium ion transport Source: RGD
  5. protein heterooligomerization Source: RGD
  6. protein homooligomerization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Metal-binding, Potassium, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily D member 2
Alternative name(s):
RK5
Shal1
Voltage-gated potassium channel subunit Kv4.2
Gene namesi
Name:Kcnd2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi68393. Kcnd2.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell projectiondendrite 1 Publication
Note: Interaction with DPP6, DLG4 or NCS1/FREQ may increase cell surface expression. Localized preferentially to the dendrites of pyramidal cells.

GO - Cellular componenti

  1. dendrite Source: RGD
  2. dendritic spine Source: RGD
  3. integral component of plasma membrane Source: RGD
  4. neuronal cell body Source: RGD
  5. neuron projection Source: RGD
  6. perinuclear endoplasmic reticulum Source: RGD
  7. plasma membrane Source: RGD
  8. postsynaptic density Source: RGD
  9. potassium channel complex Source: RGD
  10. voltage-gated potassium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi7 – 115Missing: Greatly reduces interaction with KCNIP1. 1 Publication
Mutagenesisi8 – 81W → A: Abolishes interaction with KCNP1; when associated with A-11. 1 Publication
Mutagenesisi11 – 111F → A: Abolishes interaction with KCNP1; when associated with A-8. 1 Publication
Mutagenesisi66 – 661L → R: Abolishes expression. 1 Publication
Mutagenesisi71 – 711E → K: Abolishes interaction with KCNIP1. 1 Publication
Mutagenesisi73 – 731D → M: Abolishes interaction with KCNIP1. 1 Publication
Mutagenesisi74 – 741F → R: Abolishes interaction with KCNIP1. 1 Publication
Mutagenesisi79 – 791E → L or R: Abolishes interaction with KCNIP1. 1 Publication
Mutagenesisi93 – 931R → A: Greatly reduces expression and changes multimerization. 1 Publication
Mutagenesisi481 – 4822Missing: Loss of dendritic targeted expression. 1 Publication
Mutagenesisi627 – 6304Missing: Abolishes interaction with DLG4. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 630630Potassium voltage-gated channel subfamily D member 2PRO_0000054067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381PhosphothreonineBy similarity
Modified residuei552 – 5521Phosphoserine1 Publication
Modified residuei602 – 6021PhosphothreonineBy similarity
Modified residuei607 – 6071PhosphothreonineBy similarity
Modified residuei616 – 6161PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on serine and threonine residues.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63881.
PRIDEiQ63881.

PTM databases

PhosphoSiteiQ63881.

Expressioni

Tissue specificityi

Highly expressed in heart and throughout the brain, with similar levels in cortex and hypothalamus, and much higher levels in hippocampus, dentate gyrus and the habenular nucleus of the thalamus. Detected at similar levels in heart atrium and ventricle. Detected in aorta, cardiac and smooth muscle.2 Publications

Gene expression databases

GenevestigatoriQ63881.

Interactioni

Subunit structurei

Homotetramer or heterotetramer with KCND1 and/or KCND3. Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4. Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. The KCND2-KCNIP2 channel complex contains four KCND2 and four KCNIP2 subunits. Interacts with FLNA and FLNC By similarity. Interacts with DPP6, DPP10, DLG4, NCS1/FREQ and DLG1.By similarity10 Publications

Protein-protein interaction databases

BioGridi249292. 2 interactions.
IntActiQ63881. 3 interactions.
MINTiMINT-104113.
STRINGi10116.ENSRNOP00000039227.

Structurei

Secondary structure

630
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 66
Helixi9 – 179
Beta strandi43 – 475
Beta strandi50 – 545
Helixi56 – 605
Beta strandi64 – 663
Helixi70 – 756
Helixi78 – 803
Beta strandi81 – 855
Turni89 – 913
Helixi92 – 10110
Helixi112 – 12211
Helixi131 – 14515

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NN7X-ray2.10A42-146[»]
1S6CX-ray2.00B1-30[»]
ProteinModelPortaliQ63881.
SMRiQ63881. Positions 3-146.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63881.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 183183CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini246 – 25914CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini311 – 32313CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini406 – 630225CytoplasmicSequence AnalysisAdd
BLAST

Intramembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei363 – 38321Pore-forming; Name=Segment H5Sequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei184 – 20421Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Transmembranei225 – 24521Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Transmembranei260 – 28021Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Transmembranei290 – 31021Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Transmembranei324 – 34421Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Transmembranei385 – 40521Helical; Name=Segment S6Sequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2019Interaction with KCNIP2By similarityAdd
BLAST
Regioni7 – 115Interaction with KCNIP1
Regioni71 – 9020Interaction with KCNIP1Add
BLAST
Regioni474 – 48916Mediates dendritic targetingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi370 – 3756Selectivity filterBy similarity

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOGENOMiHOG000231013.
HOVERGENiHBG106687.
InParanoidiQ63881.
KOiK04892.
PhylomeDBiQ63881.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003975. K_chnl_volt-dep_Kv4.
IPR004055. K_chnl_volt-dep_Kv4.2.
IPR024587. K_chnl_volt-dep_Kv4_C.
IPR021645. Shal-type.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF11879. DUF3399. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11601. Shal-type. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01517. KV42CHANNEL.
PR01491. KVCHANNEL.
PR01497. SHALCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63881-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAGVAAWLP FARAAAIGWM PVASGPMPAP PRQERKRTQD ALIVLNVSGT
60 70 80 90 100
RFQTWQDTLE RYPDTLLGSS ERDFFYHPET QQYFFDRDPD IFRHILNFYR
110 120 130 140 150
TGKLHYPRHE CISAYDEELA FFGLIPEIIG DCCYEEYKDR RRENAERLQD
160 170 180 190 200
DADTDNTGES ALPTMTARQR VWRAFENPHT STMALVFYYV TGFFIAVSVI
210 220 230 240 250
ANVVETVPCG SSPGHIKELP CGERYAVAFF CLDTACVMIF TVEYLLRLAA
260 270 280 290 300
APSRYRFVRS VMSIIDVVAI LPYYIGLVMT DNEDVSGAFV TLRVFRVFRI
310 320 330 340 350
FKFSRHSQGL RILGYTLKSC ASELGFLLFS LTMAIIIFAT VMFYAEKGSS
360 370 380 390 400
ASKFTSIPAA FWYTIVTMTT LGYGDMVPKT IAGKIFGSIC SLSGVLVIAL
410 420 430 440 450
PVPVIVSNFS RIYHQNQRAD KRRAQKKARL ARIRAAKSGS ANAYMQSKRN
460 470 480 490 500
GLLSNQLQSS EDEPAFVSKS GSSFETQHHH LLHCLEKTTN HEFVDEQVFE
510 520 530 540 550
ESCMEVATVN RPSSHSPSLS SQQGVTSTCC SRRHKKSFRI PNANVSGSHR
560 570 580 590 600
GSVQELSTIQ IRCVERTPLS NSRSSLNAKM EECVKLNCEQ PYVTTAIISI
610 620 630
PTPPVTTPEG DDRPESPEYS GGNIVRVSAL
Length:630
Mass (Da):70,549
Last modified:November 1, 1996 - v1
Checksum:iFDE57E8A5113BABF
GO

Sequence cautioni

The sequence AAA40929.1 differs from that shown. Reason: Frameshift at position 477.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S64320 mRNA. Translation: AAB19939.1.
M59980 mRNA. Translation: AAA40929.1. Frameshift.
PIRiI57681.
JU0271.
RefSeqiNP_113918.2. NM_031730.2.
UniGeneiRn.87841.

Genome annotation databases

GeneIDi65180.
KEGGirno:65180.
UCSCiRGD:68393. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S64320 mRNA. Translation: AAB19939.1 .
M59980 mRNA. Translation: AAA40929.1 . Frameshift.
PIRi I57681.
JU0271.
RefSeqi NP_113918.2. NM_031730.2.
UniGenei Rn.87841.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NN7 X-ray 2.10 A 42-146 [» ]
1S6C X-ray 2.00 B 1-30 [» ]
ProteinModelPortali Q63881.
SMRi Q63881. Positions 3-146.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249292. 2 interactions.
IntActi Q63881. 3 interactions.
MINTi MINT-104113.
STRINGi 10116.ENSRNOP00000039227.

Chemistry

ChEMBLi CHEMBL1075227.
GuidetoPHARMACOLOGYi 553.

PTM databases

PhosphoSitei Q63881.

Proteomic databases

PaxDbi Q63881.
PRIDEi Q63881.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 65180.
KEGGi rno:65180.
UCSCi RGD:68393. rat.

Organism-specific databases

CTDi 3751.
RGDi 68393. Kcnd2.

Phylogenomic databases

eggNOGi COG1226.
HOGENOMi HOG000231013.
HOVERGENi HBG106687.
InParanoidi Q63881.
KOi K04892.
PhylomeDBi Q63881.

Miscellaneous databases

EvolutionaryTracei Q63881.
NextBioi 614080.
PROi Q63881.

Gene expression databases

Genevestigatori Q63881.

Family and domain databases

Gene3Di 1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003975. K_chnl_volt-dep_Kv4.
IPR004055. K_chnl_volt-dep_Kv4.2.
IPR024587. K_chnl_volt-dep_Kv4_C.
IPR021645. Shal-type.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view ]
PANTHERi PTHR11537. PTHR11537. 1 hit.
Pfami PF02214. BTB_2. 1 hit.
PF11879. DUF3399. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11601. Shal-type. 1 hit.
[Graphical view ]
PRINTSi PR00169. KCHANNEL.
PR01517. KV42CHANNEL.
PR01491. KVCHANNEL.
PR01497. SHALCHANNEL.
SMARTi SM00225. BTB. 1 hit.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channel."
    Baldwin T.J., Tsaur M.-L., Lopez G.A., Jan Y.N., Jan L.Y.
    Neuron 7:471-483(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Hippocampus.
  2. "Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart."
    Roberds S.L., Tamkun M.M.
    Proc. Natl. Acad. Sci. U.S.A. 88:1798-1802(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Heart.
  3. Cited for: INTERACTION WITH KCNIP1; KCNIP2 AND KCNIP3.
  4. "A role for frequenin, a Ca2+-binding protein, as a regulator of Kv4 K+-currents."
    Nakamura T.Y., Pountney D.J., Ozaita A., Nandi S., Ueda S., Rudy B., Coetzee W.A.
    Proc. Natl. Acad. Sci. U.S.A. 98:12808-12813(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNIP1 AND NCS1.
  5. "Molecular cloning and characterization of CALP/KChIP4, a novel EF-hand protein interacting with presenilin 2 and voltage-gated potassium channel subunit Kv4."
    Morohashi Y., Hatano N., Ohya S., Takikawa R., Watabiki T., Takasugi N., Imaizumi Y., Tomita T., Iwatsubo T.
    J. Biol. Chem. 277:14965-14975(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNIP4.
  6. "Cell surface targeting and clustering interactions between heterologously expressed PSD-95 and the Shal voltage-gated potassium channel, Kv4.2."
    Wong W., Newell E.W., Jugloff D.G.M., Jones O.T., Schlichter L.C.
    J. Biol. Chem. 277:20423-20430(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF 627-VAL--LEU-630, INTERACTION WITH DLG4.
  7. "A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels."
    Shibata R., Misonou H., Campomanes C.R., Anderson A.E., Schrader L.A., Doliveira L.C., Carroll K.I., Sweatt J.D., Rhodes K.J., Trimmer J.S.
    J. Biol. Chem. 278:36445-36454(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-552.
  8. "An evolutionarily conserved dileucine motif in Shal K+ channels mediates dendritic targeting."
    Rivera J.F., Ahmad S., Quick M.W., Liman E.R., Arnold D.B.
    Nat. Neurosci. 6:243-250(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DENDRITIC TARGETING REGION, MUTAGENESIS OF 481-LEU--LEU-482.
  9. "The CD26-related dipeptidyl aminopeptidase-like protein DPPX is a critical component of neuronal A-type K+ channels."
    Nadal M.S., Ozaita A., Amarillo Y., Vega-Saenz de Miera E., Ma Y., Mo W., Goldberg E.M., Misumi Y., Ikehara Y., Neubert T.A., Rudy B.
    Neuron 37:449-461(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DPP6.
  10. "KChIPs and Kv4 alpha subunits as integral components of A-type potassium channels in mammalian brain."
    Rhodes K.J., Carroll K.I., Sung M.A., Doliveira L.C., Monaghan M.M., Burke S.L., Strassle B.W., Buchwalder L., Menegola M., Cao J., An W.F., Trimmer J.S.
    J. Neurosci. 24:7903-7915(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCNIP1 AND KCNIP3.
  11. Cited for: INTERACTION WITH KCNIP1, MUTAGENESIS OF 7-ALA--PHE-11; GLU-71; ASP-73; PHE-74 AND GLU-79.
  12. Cited for: INTERACTION WITH DPP6 AND DPP10.
  13. "Kv4 potassium channels form a tripartite complex with the anchoring protein SAP97 and CaMKII in cardiac myocytes."
    El-Haou S., Balse E., Neyroud N., Dilanian G., Gavillet B., Abriel H., Coulombe A., Jeromin A., Hatem S.N.
    Circ. Res. 104:758-769(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG1.
  14. "Determining the basis of channel-tetramerization specificity by X-ray crystallography and a sequence-comparison algorithm: family values (FamVal)."
    Nanao M.H., Zhou W., Pfaffinger P.J., Choe S.
    Proc. Natl. Acad. Sci. U.S.A. 100:8670-8675(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 42-146, MUTAGENESIS OF LEU-66 AND ARG-93.
  15. "Structural insights into the functional interaction of KChIP1 with Shal-type K(+) channels."
    Zhou W., Qian Y., Kunjilwar K., Pfaffinger P.J., Choe S.
    Neuron 41:573-586(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-30, INTERACTION WITH KCNIP1; KCNIP2 AND KCNIP3, MUTAGENESIS OF TRP-8 AND PHE-11.

Entry informationi

Entry nameiKCND2_RAT
AccessioniPrimary (citable) accession number: Q63881
Secondary accession number(s): Q00090, Q99249
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3