ID RPAB4_MOUSE Reviewed; 58 AA. AC Q63871; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1997, sequence version 2. DT 27-MAR-2024, entry version 173. DE RecName: Full=DNA-directed RNA polymerases I, II, and III subunit RPABC4; DE Short=RNA polymerases I, II, and III subunit ABC4; DE AltName: Full=DNA-directed RNA polymerase II subunit K; DE AltName: Full=Metallothionein-I gene transcription activator; DE AltName: Full=RPB10alpha; GN Name=Polr2k; Synonyms=Mt1a; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=8329119; DOI=10.1089/dna.1993.12.517; RA Xu C.; RT "cDNA cloning of a mouse factor that activates transcription from a metal RT response element of the mouse metallothionein-I gene in yeast."; RL DNA Cell Biol. 12:517-525(1993). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Mammary gland; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of CC DNA into RNA using the four ribonucleoside triphosphates as substrates. CC Common component of RNA polymerases I, II and III which synthesize CC ribosomal RNA precursors, mRNA precursors and many functional non- CC coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. CC {ECO:0000250|UniProtKB:P40422, ECO:0000250|UniProtKB:P53803}. CC -!- SUBUNIT: Component of the RNA polymerase I (Pol I), RNA polymerase II CC (Pol II) and RNA polymerase III (Pol III) complexes consisting of at CC least 13, 12 and 17 subunits, respectively (By similarity). Pol I CC complex consists of a ten-subunit catalytic core composed of CC POLR1A/RPA1, POLR1B/RPA2, POLR1C/RPAC1, POLR1D/RPAC2, POLR1H/RPA12, CC POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and CC POLR2L/RPABC5; a mobile stalk subunit POLR1F/RPA43 protruding from the CC core and additional subunits homologous to general transcription CC factors POLR1E/RPA49 and POLR1G/RPA34. Part of Pol I pre-initiation CC complex (PIC), in which Pol I core assembles with RRN3 and promoter- CC bound UTBF and SL1/TIF-IB complex (By similarity). Pol II complex CC contains a ten-subunit catalytic core composed of POLR2A/RPB1, CC POLR2B/RPB2, POLR2C/RPB3, POLR2I/RPB9, POLR2J/RPB11, POLR2E/RPABC1, CC POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5 and a CC mobile stalk composed of two subunits POLR2D/RPB4 and POLR2G/RPB7. Part CC of Pol II(G) complex, in which Pol II core associates with an CC additional subunit POLR2M; unlike conventional Pol II, Pol II(G) CC functions as a transcriptional repressor. Part of TBP-based Pol II pre- CC initiation complex (PIC), in which Pol II core assembles with general CC transcription factors and other specific initiation factors including CC GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, CC GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit CC PIC complex mediates DNA unwinding and targets Pol II core to the CC transcription start site where the first phosphodiester bond forms (By CC similarity). Pol III complex consists of a ten-subunit catalytic core CC composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, CC POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, CC POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two CC subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and CC functioning primarily in transcription initiation; and additional CC subunits homologous to general transcription factors of the RNA CC polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 CC heterotrimer required for transcription initiation and POLR3D/RPC4- CC POLR3E/RPC5 heterodimer involved in both transcription initiation and CC termination (By similarity). {ECO:0000250|UniProtKB:P40422, CC ECO:0000250|UniProtKB:P53803}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P53803}. Nucleus, CC nucleolus {ECO:0000250|UniProtKB:P53803}. CC -!- SIMILARITY: Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA CC polymerase subunit family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAB27565.2; Type=Erroneous initiation; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; S63758; AAB27565.2; ALT_INIT; mRNA. DR EMBL; BC028543; AAH28543.1; -; mRNA. DR CCDS; CCDS56980.1; -. DR RefSeq; NP_075616.2; NM_023127.3. DR AlphaFoldDB; Q63871; -. DR SMR; Q63871; -. DR STRING; 10090.ENSMUSP00000051968; -. DR PhosphoSitePlus; Q63871; -. DR MaxQB; Q63871; -. DR PaxDb; 10090-ENSMUSP00000051968; -. DR ProteomicsDB; 262700; -. DR Pumba; Q63871; -. DR DNASU; 17749; -. DR Ensembl; ENSMUST00000180159.8; ENSMUSP00000136975.2; ENSMUSG00000045996.13. DR GeneID; 17749; -. DR KEGG; mmu:17749; -. DR UCSC; uc007vml.2; mouse. DR AGR; MGI:102725; -. DR CTD; 5440; -. DR MGI; MGI:102725; Polr2k. DR VEuPathDB; HostDB:ENSMUSG00000045996; -. DR eggNOG; KOG3507; Eukaryota. DR GeneTree; ENSGT00390000008918; -. DR HOGENOM; CLU_179456_1_0_1; -. DR InParanoid; Q63871; -. DR OrthoDB; 5489148at2759; -. DR PhylomeDB; Q63871; -. DR Reactome; R-MMU-112382; Formation of RNA Pol II elongation complex. DR Reactome; R-MMU-113418; Formation of the Early Elongation Complex. DR Reactome; R-MMU-5250924; B-WICH complex positively regulates rRNA expression. DR Reactome; R-MMU-674695; RNA Polymerase II Pre-transcription Events. DR Reactome; R-MMU-6781823; Formation of TC-NER Pre-Incision Complex. DR Reactome; R-MMU-6782135; Dual incision in TC-NER. DR Reactome; R-MMU-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER. DR Reactome; R-MMU-6796648; TP53 Regulates Transcription of DNA Repair Genes. DR Reactome; R-MMU-6803529; FGFR2 alternative splicing. DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes. DR Reactome; R-MMU-72086; mRNA Capping. DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway. DR Reactome; R-MMU-72165; mRNA Splicing - Minor Pathway. DR Reactome; R-MMU-72203; Processing of Capped Intron-Containing Pre-mRNA. DR Reactome; R-MMU-73762; RNA Polymerase I Transcription Initiation. DR Reactome; R-MMU-73772; RNA Polymerase I Promoter Escape. DR Reactome; R-MMU-73776; RNA Polymerase II Promoter Escape. DR Reactome; R-MMU-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening. DR Reactome; R-MMU-73863; RNA Polymerase I Transcription Termination. DR Reactome; R-MMU-75953; RNA Polymerase II Transcription Initiation. DR Reactome; R-MMU-75955; RNA Polymerase II Transcription Elongation. DR Reactome; R-MMU-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance. DR Reactome; R-MMU-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter. DR Reactome; R-MMU-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter. DR Reactome; R-MMU-76071; RNA Polymerase III Transcription Initiation From Type 3 Promoter. DR Reactome; R-MMU-77075; RNA Pol II CTD phosphorylation and interaction with CE. DR Reactome; R-MMU-9018519; Estrogen-dependent gene expression. DR BioGRID-ORCS; 17749; 7 hits in 41 CRISPR screens. DR ChiTaRS; Polr2k; mouse. DR PRO; PR:Q63871; -. DR Proteomes; UP000000589; Chromosome 15. DR RNAct; Q63871; Protein. DR Bgee; ENSMUSG00000045996; Expressed in embryonic facial prominence and 82 other cell types or tissues. DR ExpressionAtlas; Q63871; baseline and differential. DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome. DR GO; GO:0005634; C:nucleus; ISS:UniProtKB. DR GO; GO:0005736; C:RNA polymerase I complex; IBA:GO_Central. DR GO; GO:0005665; C:RNA polymerase II, core complex; ISS:UniProtKB. DR GO; GO:0005666; C:RNA polymerase III complex; IBA:GO_Central. DR GO; GO:0003677; F:DNA binding; IEA:InterPro. DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0006360; P:transcription by RNA polymerase I; IEA:GOC. DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:MGI. DR GO; GO:0006383; P:transcription by RNA polymerase III; IEA:GOC. DR Gene3D; 2.20.28.30; RNA polymerase ii, chain L; 1. DR InterPro; IPR006591; RNAP_P/RPABC4. DR InterPro; IPR039747; RPABC4. DR InterPro; IPR029040; RPABC4/Spt4. DR PANTHER; PTHR12056; DNA-DIRECTED RNA POLYMERASES I, II, AND III; 1. DR PANTHER; PTHR12056:SF4; DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC4; 1. DR Pfam; PF03604; DNA_RNApol_7kD; 1. DR SMART; SM00659; RPOLCX; 1. DR SUPFAM; SSF63393; RNA polymerase subunits; 1. DR Genevisible; Q63871; MM. PE 3: Inferred from homology; KW DNA-directed RNA polymerase; Metal-binding; Nucleus; Reference proteome; KW Transcription; Zinc; Zinc-finger. FT CHAIN 1..58 FT /note="DNA-directed RNA polymerases I, II, and III subunit FT RPABC4" FT /id="PRO_0000159751" FT ZN_FING 19..39 FT /note="C4-type" FT /evidence="ECO:0000255" FT BINDING 19 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000250|UniProtKB:P53803" FT BINDING 22 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000250|UniProtKB:P53803" FT BINDING 36 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000250|UniProtKB:P53803" FT BINDING 39 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000250|UniProtKB:P53803" SQ SEQUENCE 58 AA; 6974 MW; D69BB6B37416F036 CRC64; MDAQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR //