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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC4

Gene

Polr2k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively (By similarity).By similarity
Factor that activates expression from a metal response element of the mouse metallothionein-I gene.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi19 – 191ZincBy similarity
Metal bindingi22 – 221ZincBy similarity
Metal bindingi36 – 361ZincBy similarity
Metal bindingi39 – 391ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri19 – 3921C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-113418. Formation of the Early Elongation Complex.
R-MMU-1834949. Cytosolic sensors of pathogen-associated DNA.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-5578749. Transcriptional regulation by small RNAs.
R-MMU-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6781823. Formation of TC-NER Pre-Incision Complex.
R-MMU-6782135. Dual incision in TC-NER.
R-MMU-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-6803529. FGFR2 alternative splicing.
R-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-72086. mRNA Capping.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72165. mRNA Splicing - Minor Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73776. RNA Polymerase II Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-MMU-73863. RNA Polymerase I Transcription Termination.
R-MMU-75953. RNA Polymerase II Transcription Initiation.
R-MMU-75955. RNA Polymerase II Transcription Elongation.
R-MMU-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-MMU-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-MMU-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-MMU-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Short name:
RNA polymerases I, II, and III subunit ABC4
Alternative name(s):
DNA-directed RNA polymerase II subunit K
Metallothionein-I gene transcription activator
RPB10alpha
Gene namesi
Name:Polr2k
Synonyms:Mt1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:102725. Polr2k.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5858DNA-directed RNA polymerases I, II, and III subunit RPABC4PRO_0000159751Add
BLAST

Proteomic databases

EPDiQ63871.
MaxQBiQ63871.
PaxDbiQ63871.
PRIDEiQ63871.

Expressioni

Gene expression databases

BgeeiQ63871.
CleanExiMM_POLR2K.
ExpressionAtlasiQ63871. baseline and differential.
GenevisibleiQ63871. MM.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051968.

Structurei

3D structure databases

ProteinModelPortaliQ63871.
SMRiQ63871. Positions 15-58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri19 – 3921C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3507. Eukaryota.
COG1996. LUCA.
GeneTreeiENSGT00390000008918.
HOVERGENiHBG054239.
InParanoidiQ63871.
OrthoDBiEOG73540T.
PhylomeDBiQ63871.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK

RLVVFDAR
Length:58
Mass (Da):6,974
Last modified:November 1, 1997 - v2
Checksum:iD69BB6B37416F036
GO

Sequence cautioni

The sequence AAB27565.2 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63758 mRNA. Translation: AAB27565.2. Different initiation.
BC028543 mRNA. Translation: AAH28543.1.
CCDSiCCDS56980.1.
RefSeqiNP_075616.2. NM_023127.3.
UniGeneiMm.27375.

Genome annotation databases

EnsembliENSMUST00000180159; ENSMUSP00000136975; ENSMUSG00000045996.
GeneIDi17749.
KEGGimmu:17749.
UCSCiuc007vml.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63758 mRNA. Translation: AAB27565.2. Different initiation.
BC028543 mRNA. Translation: AAH28543.1.
CCDSiCCDS56980.1.
RefSeqiNP_075616.2. NM_023127.3.
UniGeneiMm.27375.

3D structure databases

ProteinModelPortaliQ63871.
SMRiQ63871. Positions 15-58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051968.

Proteomic databases

EPDiQ63871.
MaxQBiQ63871.
PaxDbiQ63871.
PRIDEiQ63871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000180159; ENSMUSP00000136975; ENSMUSG00000045996.
GeneIDi17749.
KEGGimmu:17749.
UCSCiuc007vml.2. mouse.

Organism-specific databases

CTDi5440.
MGIiMGI:102725. Polr2k.

Phylogenomic databases

eggNOGiKOG3507. Eukaryota.
COG1996. LUCA.
GeneTreeiENSGT00390000008918.
HOVERGENiHBG054239.
InParanoidiQ63871.
OrthoDBiEOG73540T.
PhylomeDBiQ63871.

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-113418. Formation of the Early Elongation Complex.
R-MMU-1834949. Cytosolic sensors of pathogen-associated DNA.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-5578749. Transcriptional regulation by small RNAs.
R-MMU-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6781823. Formation of TC-NER Pre-Incision Complex.
R-MMU-6782135. Dual incision in TC-NER.
R-MMU-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-6803529. FGFR2 alternative splicing.
R-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-72086. mRNA Capping.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72165. mRNA Splicing - Minor Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73776. RNA Polymerase II Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-MMU-73863. RNA Polymerase I Transcription Termination.
R-MMU-75953. RNA Polymerase II Transcription Initiation.
R-MMU-75955. RNA Polymerase II Transcription Elongation.
R-MMU-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-MMU-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-MMU-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
R-MMU-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiQ63871.
SOURCEiSearch...

Gene expression databases

BgeeiQ63871.
CleanExiMM_POLR2K.
ExpressionAtlasiQ63871. baseline and differential.
GenevisibleiQ63871. MM.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of a mouse factor that activates transcription from a metal response element of the mouse metallothionein-I gene in yeast."
    Xu C.
    DNA Cell Biol. 12:517-525(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.

Entry informationi

Entry nameiRPAB4_MOUSE
AccessioniPrimary (citable) accession number: Q63871
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.