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Q63850 (NUP62_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear pore glycoprotein p62
Alternative name(s):
62 kDa nucleoporin
Nucleoporin Nup62
Gene names
Name:Nup62
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex By similarity.

Subunit structure

Component of the p62 complex, a complex at least composed of NUP62, NUP54, and NUPL1. Interacts with Hikeshi By similarity.

Subcellular location

Nucleusnuclear pore complex. Cytoplasmcytoskeletonspindle pole By similarity. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle By similarity.

Domain

Contains F-X-F-G repeats.

Post-translational modification

O-glycosylated.

The the inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC.

Sequence similarities

Belongs to the nucleoporin NSP1/NUP62 family.

Ontologies

Keywords
   Biological processmRNA transport
Protein transport
Translocation
Transport
   Cellular componentCytoplasm
Cytoskeleton
Nuclear pore complex
Nucleus
   DomainCoiled coil
Repeat
   PTMAcetylation
Disulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell aging

Inferred from direct assay PubMed 15572682. Source: MGI

cell death

Inferred from sequence or structural similarity. Source: UniProtKB

cell migration

Non-traceable author statement PubMed 11031258. Source: UniProtKB

cell surface receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

mRNA transport

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of MAP kinase activity

Inferred from mutant phenotype PubMed 11489947. Source: UniProtKB

negative regulation of Ras protein signal transduction

Inferred from mutant phenotype PubMed 11489947. Source: UniProtKB

negative regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 11489947. Source: UniProtKB

negative regulation of epidermal growth factor receptor signaling pathway

Inferred from mutant phenotype PubMed 11489947. Source: UniProtKB

negative regulation of programmed cell death

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

nuclear membrane

Inferred from direct assay PubMed 22683860. Source: MGI

nuclear pore

Inferred from sequence or structural similarity. Source: UniProtKB

ribonucleoprotein complex

Inferred from sequence orthology PubMed 18809582. Source: MGI

spindle pole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionPTB domain binding

Inferred from physical interaction PubMed 11031258. Source: UniProtKB

SH2 domain binding

Inferred from sequence or structural similarity. Source: UniProtKB

receptor signaling complex scaffold activity

Inferred from sequence or structural similarity. Source: UniProtKB

structural constituent of nuclear pore

Inferred from electronic annotation. Source: InterPro

thyroid hormone receptor binding

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 526525Nuclear pore glycoprotein p62
PRO_0000204881

Regions

Coiled coil332 – 462131 Potential
Compositional bias17 – 295279Thr-rich
Compositional bias202 – 335134Ala-rich
Compositional bias271 – 28414Poly-Thr
Compositional bias292 – 2954Poly-Thr

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue4121Phosphoserine Ref.3
Glycosylation3771O-linked (GlcNAc) By similarity
Glycosylation4721O-linked (GlcNAc) By similarity
Disulfide bond479Interchain (with NUP155) Probable
Disulfide bond510Interchain (with NUP155) Probable

Experimental info

Mutagenesis4791C → S: Loss of reduction of influx rate of NLS cargo upon oxidative stress; when associated with S-509. Ref.4
Mutagenesis5101C → S: Loss of reduction of influx rate of NLS cargo upon oxidative stress; when associated with S-478. Ref.4
Sequence conflict1131G → V in AAB19953. Ref.1
Sequence conflict3191A → V in AAB19953. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q63850 [UniParc].

Last modified May 27, 2002. Version 2.
Checksum: 54AE74B211018FE4

FASTA52653,255
        10         20         30         40         50         60 
MSGFNFGGTG APAGGFTFGT AKTATTTPAT GFSFSASGTG TGGFNFGTPS QPAATTPSTS 

        70         80         90        100        110        120 
LFSLTTQTPT TQTPGFNFGT TPASGGTGFS LGISTPKLSL SNAAATPATA NTGSFGLGSS 

       130        140        150        160        170        180 
TLTNAISSGS TSNQGTAPTG FVFGSSTTSA PSTGSTGFSF TSGSASQPGA SGFSLGSVGS 

       190        200        210        220        230        240 
SAQPTALSGS PFTPATLVTT TAGATQPAAA APTAATTSAG STLFASIAAA PASSSATGLS 

       250        260        270        280        290        300 
LPAPVTTAAT PSAGTLGFSL KAPGAAPGAS TTSTTTTTTT TTTTAAAAAA STTTTGFALS 

       310        320        330        340        350        360 
LKPLVSAGPS SVAATALPAS STAAGTATGP AMTYAQLESL INKWSLELED QERHFLQQAT 

       370        380        390        400        410        420 
QVNAWDRTLI ENGEKITSLH REVEKVKLDQ KRLDQELDFI LSQQKELEDL LSPLEESVKE 

       430        440        450        460        470        480 
QSGTIYLQHA DEEREKTYKL AENIDAQLKR MAQDLKDIIE HLNMAGGPAD TSDPLQQICK 

       490        500        510        520 
ILNAHMDSLQ WVDQSSALLQ RRVEEASRVC EGRRKEQERS LRIAFD 

« Hide

References

« Hide 'large scale' references
[1]"Nuclear pore complex glycoprotein p62 of Xenopus laevis and mouse: cDNA cloning and identification of its glycosylated region."
Cordes V., Waizenegger I., Krohne G.
Eur. J. Cell Biol. 55:31-47(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[4]"Intermolecular disulfide bonds between nucleoporins regulate karyopherin-dependent nuclear transport."
Yoshimura S.H., Otsuka S., Kumeta M., Taga M., Takeyasu K.
J. Cell Sci. 126:3141-3150(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS, MUTAGENESIS OF CYS-479 AND CYS-510.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S59342 mRNA. Translation: AAB19953.1.
BC005784 mRNA. Translation: AAH05784.1.
PIRA56573.
RefSeqNP_444304.1. NM_053074.1.
UniGeneMm.2565.
Mm.440497.

3D structure databases

ProteinModelPortalQ63850.
SMRQ63850. Positions 365-420.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201879. 6 interactions.
IntActQ63850. 1 interaction.
MINTMINT-4104316.

PTM databases

PhosphoSiteQ63850.

Proteomic databases

PaxDbQ63850.
PRIDEQ63850.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000057195; ENSMUSP00000056785; ENSMUSG00000043858.
ENSMUST00000107891; ENSMUSP00000103523; ENSMUSG00000043858.
GeneID18226.
KEGGmmu:18226.
UCSCuc009gqw.2. mouse.

Organism-specific databases

CTD23636.
MGIMGI:1351500. Nup62.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00730000111010.
HOGENOMHOG000007693.
HOVERGENHBG052699.
InParanoidQ63850.
KOK14306.
OMAWINTSSK.
OrthoDBEOG7PZRZB.
PhylomeDBQ63850.
TreeFamTF324795.

Gene expression databases

ArrayExpressQ63850.
BgeeQ63850.
CleanExMM_NUP62.
GenevestigatorQ63850.

Family and domain databases

InterProIPR026010. NSP1/NUP62.
IPR007758. Nucleoporin_NSP1_C.
[Graphical view]
PANTHERPTHR12084. PTHR12084. 1 hit.
PfamPF05064. Nsp1_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293652.
PROQ63850.
SOURCESearch...

Entry information

Entry nameNUP62_MOUSE
AccessionPrimary (citable) accession number: Q63850
Secondary accession number(s): Q99JN7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: May 27, 2002
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot