Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear pore glycoprotein p62

Gene

Nup62

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex. Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation. It might be involved in protein recruitment to the centrosome after nuclear breakdown.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-MMU-191859. snRNP Assembly.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-4551638. SUMOylation of chromatin organization proteins.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore glycoprotein p62
Alternative name(s):
62 kDa nucleoporin
Nucleoporin Nup62
Gene namesi
Name:Nup62
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1351500. Nup62.

Subcellular locationi

  • Nucleusnuclear pore complex By similarity
  • Cytoplasmcytoskeletonspindle pole By similarity
  • Nucleus envelope By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

  • Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi479C → S: Loss of reduction of influx rate of NLS cargo upon oxidative stress; when associated with S-509. 1 Publication1
Mutagenesisi510C → S: Loss of reduction of influx rate of NLS cargo upon oxidative stress; when associated with S-478. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002048812 – 526Nuclear pore glycoprotein p62Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Glycosylationi377O-linked (GlcNAc) threonineBy similarity1
Modified residuei412PhosphoserineCombined sources1
Modified residuei422PhosphoserineBy similarity1
Glycosylationi472O-linked (GlcNAc) serineBy similarity1
Disulfide bondi479Interchain (with NUP155)1 Publication
Disulfide bondi510Interchain (with NUP155)1 Publication

Post-translational modificationi

O-glycosylated.1 Publication
The the inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC.

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ63850.
MaxQBiQ63850.
PaxDbiQ63850.
PRIDEiQ63850.

PTM databases

iPTMnetiQ63850.
PhosphoSitePlusiQ63850.

Expressioni

Gene expression databases

BgeeiENSMUSG00000109511.
CleanExiMM_NUP62.
ExpressionAtlasiQ63850. differential.
GenevisibleiQ63850. MM.

Interactioni

Subunit structurei

Component of the p62 complex, a complex at least composed of NUP62, NUP54, and NUP58. Interacts with NUTF2. Interacts with HIKESHI. Interacts with OSBPL8. Interacts with CAPG. Interact with SAS6 and TUBG1 at the centrosome.By similarity

GO - Molecular functioni

  • Hsp70 protein binding Source: MGI
  • Hsp90 protein binding Source: MGI
  • kinesin binding Source: Ensembl
  • PTB domain binding Source: UniProtKB
  • receptor signaling complex scaffold activity Source: UniProtKB
  • SH2 domain binding Source: UniProtKB
  • thyroid hormone receptor binding Source: UniProtKB
  • ubiquitin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi201879. 8 interactors.
IntActiQ63850. 1 interactor.
MINTiMINT-4104316.
STRINGi10090.ENSMUSP00000056785.

Structurei

3D structure databases

ProteinModelPortaliQ63850.
SMRiQ63850.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati6 – 71By similarity2
Repeati46 – 472By similarity2
Repeati78 – 793By similarity2
Repeati115 – 1164By similarity2
Repeati143 – 1445By similarity2

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 1445 X 2 AA repeats of F-GBy similarityAdd BLAST139
Regioni332 – 462Required for centrosome localizationBy similarityAdd BLAST131

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili332 – 462Sequence analysisAdd BLAST131

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi17 – 295Thr-richAdd BLAST279
Compositional biasi202 – 335Ala-richAdd BLAST134
Compositional biasi271 – 284Poly-ThrAdd BLAST14
Compositional biasi292 – 295Poly-Thr4

Domaini

Contains FG repeats.

Sequence similaritiesi

Belongs to the nucleoporin NSP1/NUP62 family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG2196. Eukaryota.
ENOG410XT5X. LUCA.
GeneTreeiENSGT00730000111010.
HOGENOMiHOG000007693.
HOVERGENiHBG052699.
InParanoidiQ63850.
KOiK14306.
OMAiDEMMSKQ.
OrthoDBiEOG091G0KV9.
PhylomeDBiQ63850.
TreeFamiTF324795.

Family and domain databases

InterProiView protein in InterPro
IPR026010. NSP1/NUP62.
IPR007758. Nucleoporin_NSP1_C.
IPR033072. NUP62_met.
PANTHERiPTHR12084. PTHR12084. 1 hit.
PTHR12084:SF6. PTHR12084:SF6. 1 hit.
PfamiView protein in Pfam
PF05064. Nsp1_C. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFNFGGTG APAGGFTFGT AKTATTTPAT GFSFSASGTG TGGFNFGTPS
60 70 80 90 100
QPAATTPSTS LFSLTTQTPT TQTPGFNFGT TPASGGTGFS LGISTPKLSL
110 120 130 140 150
SNAAATPATA NTGSFGLGSS TLTNAISSGS TSNQGTAPTG FVFGSSTTSA
160 170 180 190 200
PSTGSTGFSF TSGSASQPGA SGFSLGSVGS SAQPTALSGS PFTPATLVTT
210 220 230 240 250
TAGATQPAAA APTAATTSAG STLFASIAAA PASSSATGLS LPAPVTTAAT
260 270 280 290 300
PSAGTLGFSL KAPGAAPGAS TTSTTTTTTT TTTTAAAAAA STTTTGFALS
310 320 330 340 350
LKPLVSAGPS SVAATALPAS STAAGTATGP AMTYAQLESL INKWSLELED
360 370 380 390 400
QERHFLQQAT QVNAWDRTLI ENGEKITSLH REVEKVKLDQ KRLDQELDFI
410 420 430 440 450
LSQQKELEDL LSPLEESVKE QSGTIYLQHA DEEREKTYKL AENIDAQLKR
460 470 480 490 500
MAQDLKDIIE HLNMAGGPAD TSDPLQQICK ILNAHMDSLQ WVDQSSALLQ
510 520
RRVEEASRVC EGRRKEQERS LRIAFD
Length:526
Mass (Da):53,255
Last modified:May 27, 2002 - v2
Checksum:i54AE74B211018FE4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113G → V in AAB19953 (PubMed:1915419).Curated1
Sequence conflicti319A → V in AAB19953 (PubMed:1915419).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S59342 mRNA. Translation: AAB19953.1.
BC005784 mRNA. Translation: AAH05784.1.
CCDSiCCDS21216.1.
PIRiA56573.
RefSeqiNP_444304.1. NM_053074.2.
UniGeneiMm.2565.
Mm.440497.

Genome annotation databases

EnsembliENSMUST00000057195; ENSMUSP00000056785; ENSMUSG00000109511.
ENSMUST00000207103; ENSMUSP00000147181; ENSMUSG00000109511.
ENSMUST00000208626; ENSMUSP00000146695; ENSMUSG00000109511.
GeneIDi18226.
KEGGimmu:18226.
UCSCiuc009gqw.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiNUP62_MOUSE
AccessioniPrimary (citable) accession number: Q63850
Secondary accession number(s): Q99JN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: May 27, 2002
Last modified: August 30, 2017
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families