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Q63850

- NUP62_MOUSE

UniProt

Q63850 - NUP62_MOUSE

Protein

Nuclear pore glycoprotein p62

Gene

Nup62

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
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    Functioni

    Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. PTB domain binding Source: UniProtKB
    3. receptor signaling complex scaffold activity Source: UniProtKB
    4. SH2 domain binding Source: UniProtKB
    5. structural constituent of nuclear pore Source: InterPro
    6. thyroid hormone receptor binding Source: UniProtKB
    7. ubiquitin binding Source: UniProtKB

    GO - Biological processi

    1. cell aging Source: MGI
    2. cell death Source: UniProtKB
    3. cell migration Source: UniProtKB
    4. cell surface receptor signaling pathway Source: UniProtKB
    5. mRNA transport Source: UniProtKB-KW
    6. negative regulation of apoptotic process Source: UniProtKB
    7. negative regulation of cell proliferation Source: UniProtKB
    8. negative regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
    9. negative regulation of MAP kinase activity Source: UniProtKB
    10. negative regulation of programmed cell death Source: UniProtKB
    11. negative regulation of Ras protein signal transduction Source: UniProtKB
    12. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    13. positive regulation of transcription, DNA-templated Source: UniProtKB
    14. protein transport Source: UniProtKB-KW
    15. transcription, DNA-templated Source: UniProtKB

    Keywords - Biological processi

    mRNA transport, Protein transport, Translocation, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196650. Nuclear Pore Complex (NPC) Disassembly.
    REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear pore glycoprotein p62
    Alternative name(s):
    62 kDa nucleoporin
    Nucleoporin Nup62
    Gene namesi
    Name:Nup62
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1351500. Nup62.

    Subcellular locationi

    Nucleusnuclear pore complex. Cytoplasmcytoskeletonspindle pole By similarity
    Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. nuclear membrane Source: MGI
    3. nuclear pore Source: UniProtKB
    4. ribonucleoprotein complex Source: MGI
    5. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nuclear pore complex, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi479 – 4791C → S: Loss of reduction of influx rate of NLS cargo upon oxidative stress; when associated with S-509. 1 Publication
    Mutagenesisi510 – 5101C → S: Loss of reduction of influx rate of NLS cargo upon oxidative stress; when associated with S-478. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 526525Nuclear pore glycoprotein p62PRO_0000204881Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Glycosylationi377 – 3771O-linked (GlcNAc)By similarity
    Modified residuei412 – 4121Phosphoserine1 Publication
    Glycosylationi472 – 4721O-linked (GlcNAc)By similarity
    Disulfide bondi479 – 479Interchain (with NUP155)1 Publication
    Disulfide bondi510 – 510Interchain (with NUP155)1 Publication

    Post-translational modificationi

    O-glycosylated.
    The the inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC.

    Keywords - PTMi

    Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ63850.
    PaxDbiQ63850.
    PRIDEiQ63850.

    PTM databases

    PhosphoSiteiQ63850.

    Expressioni

    Gene expression databases

    ArrayExpressiQ63850.
    BgeeiQ63850.
    CleanExiMM_NUP62.
    GenevestigatoriQ63850.

    Interactioni

    Subunit structurei

    Component of the p62 complex, a complex at least composed of NUP62, NUP54, and NUPL1. Interacts with Hikeshi By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201879. 6 interactions.
    IntActiQ63850. 1 interaction.
    MINTiMINT-4104316.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63850.
    SMRiQ63850. Positions 365-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili332 – 462131Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi17 – 295279Thr-richAdd
    BLAST
    Compositional biasi202 – 335134Ala-richAdd
    BLAST
    Compositional biasi271 – 28414Poly-ThrAdd
    BLAST
    Compositional biasi292 – 2954Poly-Thr

    Domaini

    Contains F-X-F-G repeats.

    Sequence similaritiesi

    Belongs to the nucleoporin NSP1/NUP62 family.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00730000111010.
    HOGENOMiHOG000007693.
    HOVERGENiHBG052699.
    InParanoidiQ63850.
    KOiK14306.
    OMAiPAMTYAQ.
    OrthoDBiEOG7PZRZB.
    PhylomeDBiQ63850.
    TreeFamiTF324795.

    Family and domain databases

    InterProiIPR026010. NSP1/NUP62.
    IPR007758. Nucleoporin_NSP1_C.
    [Graphical view]
    PANTHERiPTHR12084. PTHR12084. 1 hit.
    PfamiPF05064. Nsp1_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q63850-1 [UniParc]FASTAAdd to Basket

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    MSGFNFGGTG APAGGFTFGT AKTATTTPAT GFSFSASGTG TGGFNFGTPS    50
    QPAATTPSTS LFSLTTQTPT TQTPGFNFGT TPASGGTGFS LGISTPKLSL 100
    SNAAATPATA NTGSFGLGSS TLTNAISSGS TSNQGTAPTG FVFGSSTTSA 150
    PSTGSTGFSF TSGSASQPGA SGFSLGSVGS SAQPTALSGS PFTPATLVTT 200
    TAGATQPAAA APTAATTSAG STLFASIAAA PASSSATGLS LPAPVTTAAT 250
    PSAGTLGFSL KAPGAAPGAS TTSTTTTTTT TTTTAAAAAA STTTTGFALS 300
    LKPLVSAGPS SVAATALPAS STAAGTATGP AMTYAQLESL INKWSLELED 350
    QERHFLQQAT QVNAWDRTLI ENGEKITSLH REVEKVKLDQ KRLDQELDFI 400
    LSQQKELEDL LSPLEESVKE QSGTIYLQHA DEEREKTYKL AENIDAQLKR 450
    MAQDLKDIIE HLNMAGGPAD TSDPLQQICK ILNAHMDSLQ WVDQSSALLQ 500
    RRVEEASRVC EGRRKEQERS LRIAFD 526
    Length:526
    Mass (Da):53,255
    Last modified:May 27, 2002 - v2
    Checksum:i54AE74B211018FE4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti113 – 1131G → V in AAB19953. (PubMed:1915419)Curated
    Sequence conflicti319 – 3191A → V in AAB19953. (PubMed:1915419)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S59342 mRNA. Translation: AAB19953.1.
    BC005784 mRNA. Translation: AAH05784.1.
    CCDSiCCDS21216.1.
    PIRiA56573.
    RefSeqiNP_444304.1. NM_053074.1.
    UniGeneiMm.2565.
    Mm.440497.

    Genome annotation databases

    EnsembliENSMUST00000057195; ENSMUSP00000056785; ENSMUSG00000043858.
    ENSMUST00000107891; ENSMUSP00000103523; ENSMUSG00000043858.
    GeneIDi18226.
    KEGGimmu:18226.
    UCSCiuc009gqw.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S59342 mRNA. Translation: AAB19953.1 .
    BC005784 mRNA. Translation: AAH05784.1 .
    CCDSi CCDS21216.1.
    PIRi A56573.
    RefSeqi NP_444304.1. NM_053074.1.
    UniGenei Mm.2565.
    Mm.440497.

    3D structure databases

    ProteinModelPortali Q63850.
    SMRi Q63850. Positions 365-420.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201879. 6 interactions.
    IntActi Q63850. 1 interaction.
    MINTi MINT-4104316.

    PTM databases

    PhosphoSitei Q63850.

    Proteomic databases

    MaxQBi Q63850.
    PaxDbi Q63850.
    PRIDEi Q63850.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000057195 ; ENSMUSP00000056785 ; ENSMUSG00000043858 .
    ENSMUST00000107891 ; ENSMUSP00000103523 ; ENSMUSG00000043858 .
    GeneIDi 18226.
    KEGGi mmu:18226.
    UCSCi uc009gqw.2. mouse.

    Organism-specific databases

    CTDi 23636.
    MGIi MGI:1351500. Nup62.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00730000111010.
    HOGENOMi HOG000007693.
    HOVERGENi HBG052699.
    InParanoidi Q63850.
    KOi K14306.
    OMAi PAMTYAQ.
    OrthoDBi EOG7PZRZB.
    PhylomeDBi Q63850.
    TreeFami TF324795.

    Enzyme and pathway databases

    Reactomei REACT_196650. Nuclear Pore Complex (NPC) Disassembly.
    REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.

    Miscellaneous databases

    NextBioi 293652.
    PROi Q63850.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q63850.
    Bgeei Q63850.
    CleanExi MM_NUP62.
    Genevestigatori Q63850.

    Family and domain databases

    InterProi IPR026010. NSP1/NUP62.
    IPR007758. Nucleoporin_NSP1_C.
    [Graphical view ]
    PANTHERi PTHR12084. PTHR12084. 1 hit.
    Pfami PF05064. Nsp1_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nuclear pore complex glycoprotein p62 of Xenopus laevis and mouse: cDNA cloning and identification of its glycosylated region."
      Cordes V., Waizenegger I., Krohne G.
      Eur. J. Cell Biol. 55:31-47(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    4. "Intermolecular disulfide bonds between nucleoporins regulate karyopherin-dependent nuclear transport."
      Yoshimura S.H., Otsuka S., Kumeta M., Taga M., Takeyasu K.
      J. Cell Sci. 126:3141-3150(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS, MUTAGENESIS OF CYS-479 AND CYS-510.

    Entry informationi

    Entry nameiNUP62_MOUSE
    AccessioniPrimary (citable) accession number: Q63850
    Secondary accession number(s): Q99JN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2002
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3