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Protein

Nuclear pore glycoprotein p62

Gene

Nup62

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex (By similarity).By similarity

GO - Molecular functioni

  1. PTB domain binding Source: UniProtKB
  2. receptor signaling complex scaffold activity Source: UniProtKB
  3. SH2 domain binding Source: UniProtKB
  4. structural constituent of nuclear pore Source: InterPro
  5. thyroid hormone receptor binding Source: UniProtKB
  6. ubiquitin binding Source: UniProtKB

GO - Biological processi

  1. cell aging Source: MGI
  2. cell death Source: UniProtKB
  3. cell migration Source: UniProtKB
  4. cell surface receptor signaling pathway Source: UniProtKB
  5. mRNA transport Source: UniProtKB-KW
  6. negative regulation of apoptotic process Source: UniProtKB
  7. negative regulation of cell proliferation Source: UniProtKB
  8. negative regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
  9. negative regulation of MAP kinase activity Source: UniProtKB
  10. negative regulation of programmed cell death Source: UniProtKB
  11. negative regulation of Ras protein signal transduction Source: UniProtKB
  12. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  13. positive regulation of transcription, DNA-templated Source: UniProtKB
  14. protein transport Source: UniProtKB-KW
  15. transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiREACT_196650. Nuclear Pore Complex (NPC) Disassembly.
REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_270446. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore glycoprotein p62
Alternative name(s):
62 kDa nucleoporin
Nucleoporin Nup62
Gene namesi
Name:Nup62
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1351500. Nup62.

Subcellular locationi

Nucleusnuclear pore complex. Cytoplasmcytoskeletonspindle pole By similarity
Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. intracellular membrane-bounded organelle Source: MGI
  3. nuclear envelope Source: MGI
  4. nuclear membrane Source: MGI
  5. nuclear pore Source: UniProtKB
  6. ribonucleoprotein complex Source: MGI
  7. spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi479 – 4791C → S: Loss of reduction of influx rate of NLS cargo upon oxidative stress; when associated with S-509. 1 Publication
Mutagenesisi510 – 5101C → S: Loss of reduction of influx rate of NLS cargo upon oxidative stress; when associated with S-478. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 526525Nuclear pore glycoprotein p62PRO_0000204881Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Glycosylationi377 – 3771O-linked (GlcNAc)By similarity
Modified residuei412 – 4121Phosphoserine1 Publication
Glycosylationi472 – 4721O-linked (GlcNAc)By similarity
Disulfide bondi479 – 479Interchain (with NUP155)1 Publication
Disulfide bondi510 – 510Interchain (with NUP155)1 Publication

Post-translational modificationi

O-glycosylated.
The the inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC.

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ63850.
PaxDbiQ63850.
PRIDEiQ63850.

PTM databases

PhosphoSiteiQ63850.

Expressioni

Gene expression databases

BgeeiQ63850.
CleanExiMM_NUP62.
ExpressionAtlasiQ63850. baseline and differential.
GenevestigatoriQ63850.

Interactioni

Subunit structurei

Component of the p62 complex, a complex at least composed of NUP62, NUP54, and NUPL1. Interacts with Hikeshi (By similarity).By similarity

Protein-protein interaction databases

BioGridi201879. 6 interactions.
IntActiQ63850. 1 interaction.
MINTiMINT-4104316.

Structurei

3D structure databases

ProteinModelPortaliQ63850.
SMRiQ63850. Positions 365-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili332 – 462131Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi17 – 295279Thr-richAdd
BLAST
Compositional biasi202 – 335134Ala-richAdd
BLAST
Compositional biasi271 – 28414Poly-ThrAdd
BLAST
Compositional biasi292 – 2954Poly-Thr

Domaini

Contains F-X-F-G repeats.

Sequence similaritiesi

Belongs to the nucleoporin NSP1/NUP62 family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00730000111010.
HOGENOMiHOG000007693.
HOVERGENiHBG052699.
InParanoidiQ63850.
KOiK14306.
OMAiHINATSQ.
OrthoDBiEOG7PZRZB.
PhylomeDBiQ63850.
TreeFamiTF324795.

Family and domain databases

InterProiIPR026010. NSP1/NUP62.
IPR007758. Nucleoporin_NSP1_C.
[Graphical view]
PANTHERiPTHR12084. PTHR12084. 1 hit.
PfamiPF05064. Nsp1_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFNFGGTG APAGGFTFGT AKTATTTPAT GFSFSASGTG TGGFNFGTPS
60 70 80 90 100
QPAATTPSTS LFSLTTQTPT TQTPGFNFGT TPASGGTGFS LGISTPKLSL
110 120 130 140 150
SNAAATPATA NTGSFGLGSS TLTNAISSGS TSNQGTAPTG FVFGSSTTSA
160 170 180 190 200
PSTGSTGFSF TSGSASQPGA SGFSLGSVGS SAQPTALSGS PFTPATLVTT
210 220 230 240 250
TAGATQPAAA APTAATTSAG STLFASIAAA PASSSATGLS LPAPVTTAAT
260 270 280 290 300
PSAGTLGFSL KAPGAAPGAS TTSTTTTTTT TTTTAAAAAA STTTTGFALS
310 320 330 340 350
LKPLVSAGPS SVAATALPAS STAAGTATGP AMTYAQLESL INKWSLELED
360 370 380 390 400
QERHFLQQAT QVNAWDRTLI ENGEKITSLH REVEKVKLDQ KRLDQELDFI
410 420 430 440 450
LSQQKELEDL LSPLEESVKE QSGTIYLQHA DEEREKTYKL AENIDAQLKR
460 470 480 490 500
MAQDLKDIIE HLNMAGGPAD TSDPLQQICK ILNAHMDSLQ WVDQSSALLQ
510 520
RRVEEASRVC EGRRKEQERS LRIAFD
Length:526
Mass (Da):53,255
Last modified:May 27, 2002 - v2
Checksum:i54AE74B211018FE4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131G → V in AAB19953 (PubMed:1915419).Curated
Sequence conflicti319 – 3191A → V in AAB19953 (PubMed:1915419).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S59342 mRNA. Translation: AAB19953.1.
BC005784 mRNA. Translation: AAH05784.1.
CCDSiCCDS21216.1.
PIRiA56573.
RefSeqiNP_444304.1. NM_053074.1.
UniGeneiMm.2565.
Mm.440497.

Genome annotation databases

EnsembliENSMUST00000057195; ENSMUSP00000056785; ENSMUSG00000043858.
ENSMUST00000107891; ENSMUSP00000103523; ENSMUSG00000043858.
GeneIDi18226.
KEGGimmu:18226.
UCSCiuc009gqw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S59342 mRNA. Translation: AAB19953.1.
BC005784 mRNA. Translation: AAH05784.1.
CCDSiCCDS21216.1.
PIRiA56573.
RefSeqiNP_444304.1. NM_053074.1.
UniGeneiMm.2565.
Mm.440497.

3D structure databases

ProteinModelPortaliQ63850.
SMRiQ63850. Positions 365-420.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201879. 6 interactions.
IntActiQ63850. 1 interaction.
MINTiMINT-4104316.

PTM databases

PhosphoSiteiQ63850.

Proteomic databases

MaxQBiQ63850.
PaxDbiQ63850.
PRIDEiQ63850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057195; ENSMUSP00000056785; ENSMUSG00000043858.
ENSMUST00000107891; ENSMUSP00000103523; ENSMUSG00000043858.
GeneIDi18226.
KEGGimmu:18226.
UCSCiuc009gqw.2. mouse.

Organism-specific databases

CTDi23636.
MGIiMGI:1351500. Nup62.

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00730000111010.
HOGENOMiHOG000007693.
HOVERGENiHBG052699.
InParanoidiQ63850.
KOiK14306.
OMAiHINATSQ.
OrthoDBiEOG7PZRZB.
PhylomeDBiQ63850.
TreeFamiTF324795.

Enzyme and pathway databases

ReactomeiREACT_196650. Nuclear Pore Complex (NPC) Disassembly.
REACT_204192. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_270446. Transcriptional regulation by small RNAs.

Miscellaneous databases

NextBioi293652.
PROiQ63850.
SOURCEiSearch...

Gene expression databases

BgeeiQ63850.
CleanExiMM_NUP62.
ExpressionAtlasiQ63850. baseline and differential.
GenevestigatoriQ63850.

Family and domain databases

InterProiIPR026010. NSP1/NUP62.
IPR007758. Nucleoporin_NSP1_C.
[Graphical view]
PANTHERiPTHR12084. PTHR12084. 1 hit.
PfamiPF05064. Nsp1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nuclear pore complex glycoprotein p62 of Xenopus laevis and mouse: cDNA cloning and identification of its glycosylated region."
    Cordes V., Waizenegger I., Krohne G.
    Eur. J. Cell Biol. 55:31-47(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Intermolecular disulfide bonds between nucleoporins regulate karyopherin-dependent nuclear transport."
    Yoshimura S.H., Otsuka S., Kumeta M., Taga M., Takeyasu K.
    J. Cell Sci. 126:3141-3150(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS, MUTAGENESIS OF CYS-479 AND CYS-510.

Entry informationi

Entry nameiNUP62_MOUSE
AccessioniPrimary (citable) accession number: Q63850
Secondary accession number(s): Q99JN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: May 27, 2002
Last modified: March 4, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.