Reviewed,
UniProtKB/Swiss-Prot Q63844 (MK03_MOUSE)
Last modified
October 13, 2009.
Version 101.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Mitogen-activated protein kinase 3 EC=2.7.11.24 Alternative name(s): Extracellular signal-regulated kinase 1 Short name=ERK-1 Insulin-stimulated MAP2 kinase MAP kinase 1 Short name=MAPK 1 p44-ERK1 ERT2 p44-MAPK Microtubule-associated protein 2 kinase MNK1 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 380 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) and heat shock factor protein 4 (HSF4) By similarity. Phosphorylates SPZ1. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium By similarity. |
| Enzyme regulation | Activated by tyrosine and threonine phosphorylation. |
| Subunit structure | Interacts with HSF4 and NISCH By similarity. Interacts with MORG1. |
| Domain | The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. |
| Post-translational modification | Dually phosphorylated on Thr-203 and Tyr-205, which activates the enzyme By similarity. Autophosphorylates on threonine and tyrosine residues in vitro. |
| Sequence similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.2 | ||||||
| Chain | 2 – 380 | 379 | Mitogen-activated protein kinase 3 | PRO_0000186252 | |||||
Regions | |||||||||
| Domain | 43 – 331 | 289 | Protein kinase | ||||||
| Nucleotide binding | 49 – 57 | 9 | ATP By similarity | ||||||
| Motif | 203 – 205 | 3 | TXY | ||||||
Sites | |||||||||
| Active site | 167 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 72 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.2 | ||||||
| Modified residue | 203 | 1 | Phosphothreonine Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 | ||||||
| Modified residue | 205 | 1 | Phosphotyrosine Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 | ||||||
Experimental info | |||||||||
| Sequence conflict | 178 | 1 | T → P in CAA45889. Ref.7 | ||||||
| Sequence conflict | 178 | 1 | T → P Ref.8 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney and Mammary tumor. |
| [2] | Bienvenut W.V., Serrels B., Brunton V.G., Frame M.C. Submitted (FEB-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-65; 89-105; 110-132; 157-209; 213-221; 280-288; 304-319 AND 361-371, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic fibroblast. |
| [3] | "Molecular cloning of a mouse extracellular signal regulated kinase (erk-1)." Tanner B., Mueckler M. Biochim. Biophys. Acta 1171:319-320(1993) [PubMed: 8424957] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-380. |
| [4] | "Mouse Erk-1 gene product is a serine/threonine protein kinase that has the potential to phosphorylate tyrosine." Crews C.M., Alessandrini A.A., Erikson R.L. Proc. Natl. Acad. Sci. U.S.A. 88:8845-8849(1991) [PubMed: 1717989] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-19. Tissue: Pre-B cell. |
| [5] | "Molecular analysis of microtubule-associated protein-2 kinase cDNA from mouse and rat brain." de Miguel C., Kligman D., Patel J., Detera-Wadleigh S.D. DNA Cell Biol. 10:505-514(1991) [PubMed: 1716439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 77-380. Tissue: Fetal brain. |
| [6] | Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 136-153 AND 191-209, MASS SPECTROMETRY. Tissue: Hippocampus. |
| [7] | "Novel CDC2-related protein kinases produced in murine hematopoietic stem cells." Ershler M.A., Nagorskaya T.V., Visser J.W.M., Belyavsky A.V. Gene 124:305-306(1993) [PubMed: 8444355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 171-209. Strain: CBA. Tissue: Bone marrow. |
| [8] | "Identification of new protein kinase genes, similar to kinases of the cdc2 family and expressed in murine hematopoietic stem cells." Ershler M.A., Nagorskaya T.V., Visser J.W.M., Belyavsky A.V. Dokl. Akad. Nauk SSSR 324:893-897(1992) [PubMed: 1459009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 171-205. |
| [9] | "Modular construction of a signaling scaffold: MORG1 interacts with components of the ERK cascade and links ERK signaling to specific agonists." Vomastek T., Schaeffer H.-J., Tarcsafalvi A., Smolkin M.E., Bissonette E.A., Weber M.J. Proc. Natl. Acad. Sci. U.S.A. 101:6981-6986(2004) [PubMed: 15118098] [Abstract] Cited for: INTERACTION WITH MORG1. |
| [10] | "bHLH-zip transcription factor Spz1 mediates mitogen-activated protein kinase cell proliferation, transformation, and tumorigenesis." Hsu S.-H., Hsieh-Li H.-M., Huang H.-Y., Huang P.-H., Li H. Cancer Res. 65:4041-4050(2005) [PubMed: 15899793] [Abstract] Cited for: PHOSPHORYLATION OF SPZ1. |
| [11] | "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203 AND TYR-205, MASS SPECTROMETRY. Tissue: Mast cell. |
| [12] | "Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M. Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203 AND TYR-205, MASS SPECTROMETRY. |
| [13] | "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203 AND TYR-205, MASS SPECTROMETRY. Tissue: Brain. |
| [14] | "Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203 AND TYR-205, MASS SPECTROMETRY. |
| [15] | "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203 AND TYR-205, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| BC013754 mRNA. Translation: AAH13754.1. BC029712 mRNA. Translation: AAH29712.1. S58470 mRNA. Translation: AAB19973.1. X64605 mRNA. Translation: CAA45889.1. | |
| IPI | IPI00230277. |
| PIR | S28184. |
| RefSeq | NP_036082.1. |
| UniGene | Mm.8385 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1PME based on UniProtKB P28482. |
| SMR | Q63844. Positions 29-373, 30-374. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q63844. |
PTM databases | |
| PhosphoSite | Q63844. |
Proteomic databases | |
| PRIDE | Q63844. |
Genome annotation databases | |
| Ensembl | ENSMUST00000050201; ENSMUSP00000101969; ENSMUSG00000063065; Mus musculus. [Genome view] ENSMUST00000057669; ENSMUSP00000051619; ENSMUSG00000063065; Mus musculus. [Genome view] |
| GeneID | 26417. |
| KEGG | mmu:26417. |
| NMPDR | fig|10090.3.peg.17639. |
| UCSC | uc009jsm.1. mouse. |
Organism-specific databases | |
| CTD | 26417. |
| MGI | MGI:1346859. Mapk3. |
Phylogenomic databases | |
| HOGENOM | Q63844. |
| HOVERGEN | Q63844. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.24. 244. |
Gene expression databases | |
| ArrayExpress | Q63844. |
| Bgee | Q63844. |
| CleanEx | MM_MAPK3. |
| Genevestigator | Q63844. |
| GermOnline | ENSMUSG00000063065. Mus musculus. |
Family and domain databases | |
| InterPro | IPR008349. Erk_1_2_MAPK. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| PRINTS | PR01770. ERK1ERK2MAPK. |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 304429. |
| SOURCE | Search... |
Entry information
| Entry name | MK03_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q63844 Secondary accession number(s): Q61531, Q8K0X5, Q91YW5 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

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