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Protein

Calcineurin subunit B type 1

Gene

Ppp3r1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Miscellaneous

This protein has four functional calcium-binding sites.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi31 – 421Add BLAST12
Calcium bindingi63 – 742Add BLAST12
Calcium bindingi100 – 1113Add BLAST12
Calcium bindingi141 – 1524Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • cyclosporin A binding Source: MGI
  • enzyme binding Source: BHF-UCL
  • phosphoprotein phosphatase activity Source: MGI
  • protein domain specific binding Source: MGI

GO - Biological processi

  • branching involved in blood vessel morphogenesis Source: MGI
  • calcineurin-NFAT signaling cascade Source: MGI
  • epithelial to mesenchymal transition Source: MGI
  • heart development Source: MGI
  • lung epithelial cell differentiation Source: MGI
  • myelination in peripheral nervous system Source: MGI
  • NFAT protein import into nucleus Source: MGI
  • positive regulation of NFAT protein import into nucleus Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein dephosphorylation Source: MGI
  • protein localization to nucleus Source: MGI
  • Schwann cell development Source: MGI

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-2871809. FCERI mediated Ca+2 mobilization.
R-MMU-4086398. Ca2+ pathway.
R-MMU-5607763. CLEC7A (Dectin-1) induces NFAT activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin subunit B type 1
Alternative name(s):
Protein phosphatase 2B regulatory subunit 1
Protein phosphatase 3 regulatory subunit B alpha isoform 1
Gene namesi
Name:Ppp3r1
Synonyms:Cnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107172. Ppp3r1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000734852 – 170Calcineurin subunit B type 1Add BLAST169

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei106PhosphotyrosineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ63810.
PeptideAtlasiQ63810.
PRIDEiQ63810.

PTM databases

iPTMnetiQ63810.
PhosphoSitePlusiQ63810.

Expressioni

Tissue specificityi

Brain specific.

Gene expression databases

BgeeiENSMUSG00000033953.
GenevisibleiQ63810. MM.

Interactioni

Subunit structurei

Composed of a catalytic subunit (A) and a regulatory subunit (B). Isoform 1 and isoform 2 interact with CIB1 (via C-terminal region); the interaction increases upon cardiomyocytes hypertrophy.

Binary interactionsi

WithEntry#Exp.IntActNotes
Map3k5O350993EBI-6666164,EBI-777493

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • protein domain specific binding Source: MGI

Protein-protein interaction databases

BioGridi202347. 1 interactor.
IntActiQ63810. 3 interactors.
MINTiMINT-1513271.
STRINGi10090.ENSMUSP00000099944.

Structurei

Secondary structure

1170
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 30Combined sources14
Helixi40 – 44Combined sources5
Helixi48 – 51Combined sources4
Helixi55 – 62Combined sources8
Beta strandi67 – 69Combined sources3
Helixi72 – 80Combined sources9
Helixi88 – 99Combined sources12
Beta strandi104 – 107Combined sources4
Helixi109 – 120Combined sources12
Helixi121 – 123Combined sources3
Helixi126 – 140Combined sources15
Beta strandi144 – 148Combined sources5
Helixi150 – 157Combined sources8
Helixi158 – 160Combined sources3
Helixi162 – 165Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ORBX-ray3.11B1-170[»]
ProteinModelPortaliQ63810.
SMRiQ63810.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 51EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini50 – 85EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini87 – 122EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini128 – 163EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00860000133729.
HOGENOMiHOG000233019.
HOVERGENiHBG105307.
InParanoidiQ63810.
KOiK06268.
OMAiVDKTMME.
OrthoDBiEOG091G0R0W.
PhylomeDBiQ63810.
TreeFamiTF105558.

Family and domain databases

CDDicd00051. EFh. 1 hit.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
PfamiView protein in Pfam
PF13499. EF-hand_7. 2 hits.
SMARTiView protein in SMART
SM00054. EFh. 4 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63810-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ
60 70 80 90 100
QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD
110 120 130 140 150
MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF
160 170
EEFCAVVGGL DIHKKMVVDV
Length:170
Mass (Da):19,300
Last modified:May 1, 2007 - v3
Checksum:iC904715DC0386056
GO
Isoform 2 (identifier: Q63810-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.

Note: No experimental confirmation available.
Show »
Length:160
Mass (Da):18,208
Checksum:iF94B070F21E70466
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3N → S in AAB23171 (PubMed:1325794).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0248421 – 10Missing in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S43864 mRNA. Translation: AAB23171.1.
AK133403 mRNA. Translation: BAE21637.1.
AK145541 mRNA. Translation: BAE26495.1.
AK160938 mRNA. Translation: BAE36102.1.
AK163241 mRNA. Translation: BAE37251.1.
AL713926, AL606466 Genomic DNA. Translation: CAI51912.1.
AL606466, AL713926 Genomic DNA. Translation: CAI51920.1.
CCDSiCCDS24448.1. [Q63810-1]
PIRiJC1220.
RefSeqiNP_077779.2. NM_024459.2. [Q63810-1]
UniGeneiMm.41840.

Genome annotation databases

EnsembliENSMUST00000102880; ENSMUSP00000099944; ENSMUSG00000033953. [Q63810-1]
GeneIDi19058.
KEGGimmu:19058.
UCSCiuc007ibz.1. mouse. [Q63810-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCANB1_MOUSE
AccessioniPrimary (citable) accession number: Q63810
Secondary accession number(s): Q3V067, Q5F225
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 2007
Last modified: August 30, 2017
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families