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Protein

Calcineurin subunit B type 1

Gene

Ppp3r1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi31 – 42121Add
BLAST
Calcium bindingi63 – 74122Add
BLAST
Calcium bindingi100 – 111123Add
BLAST
Calcium bindingi141 – 152124Add
BLAST

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • enzyme binding Source: BHF-UCL
  • phosphoprotein phosphatase activity Source: MGI
  • protein domain specific binding Source: MGI

GO - Biological processi

  • calcineurin-NFAT signaling cascade Source: MGI
  • epithelial to mesenchymal transition Source: MGI
  • heart development Source: MGI
  • lung epithelial cell differentiation Source: MGI
  • myelination in peripheral nervous system Source: MGI
  • NFAT protein import into nucleus Source: MGI
  • patterning of blood vessels Source: MGI
  • positive regulation of NFAT protein import into nucleus Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein dephosphorylation Source: MGI
  • protein localization to nucleus Source: MGI
  • Schwann cell development Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin subunit B type 1
Alternative name(s):
Protein phosphatase 2B regulatory subunit 1
Protein phosphatase 3 regulatory subunit B alpha isoform 1
Gene namesi
Name:Ppp3r1
Synonyms:Cnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107172. Ppp3r1.

Subcellular locationi

GO - Cellular componenti

  • calcineurin complex Source: MGI
  • cytosol Source: Reactome
  • sarcolemma Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 170169Calcineurin subunit B type 1PRO_0000073485Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei106 – 1061PhosphotyrosineCombined sources

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ63810.
MaxQBiQ63810.
PaxDbiQ63810.
PRIDEiQ63810.

PTM databases

iPTMnetiQ63810.
PhosphoSiteiQ63810.

Expressioni

Tissue specificityi

Brain specific.

Gene expression databases

BgeeiQ63810.
GenevisibleiQ63810. MM.

Interactioni

Subunit structurei

Composed of a catalytic subunit (A) and a regulatory subunit (B). Isoform 1 and isoform 2 interact with CIB1 (via C-terminal region); the interaction increases upon cardiomyocytes hypertrophy.

Binary interactionsi

WithEntry#Exp.IntActNotes
Map3k5O350993EBI-6666164,EBI-777493

GO - Molecular functioni

  • enzyme binding Source: BHF-UCL
  • protein domain specific binding Source: MGI

Protein-protein interaction databases

BioGridi202347. 1 interaction.
IntActiQ63810. 3 interactions.
MINTiMINT-1513271.
STRINGi10090.ENSMUSP00000099944.

Structurei

Secondary structure

1
170
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 3014Combined sources
Helixi40 – 445Combined sources
Helixi48 – 514Combined sources
Helixi55 – 628Combined sources
Beta strandi67 – 693Combined sources
Helixi72 – 809Combined sources
Helixi88 – 9912Combined sources
Beta strandi104 – 1074Combined sources
Helixi109 – 12012Combined sources
Helixi121 – 1233Combined sources
Helixi126 – 14015Combined sources
Beta strandi144 – 1485Combined sources
Helixi150 – 1578Combined sources
Helixi158 – 1603Combined sources
Helixi162 – 1654Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ORBX-ray3.11B1-170[»]
ProteinModelPortaliQ63810.
SMRiQ63810. Positions 7-161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 5134EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini50 – 8536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini87 – 12236EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini128 – 16336EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119179.
HOGENOMiHOG000233019.
HOVERGENiHBG105307.
InParanoidiQ63810.
KOiK06268.
OMAiYIGNGEL.
OrthoDBiEOG75QR5D.
PhylomeDBiQ63810.
TreeFamiTF105558.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63810-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ
60 70 80 90 100
QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD
110 120 130 140 150
MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF
160 170
EEFCAVVGGL DIHKKMVVDV
Length:170
Mass (Da):19,300
Last modified:May 1, 2007 - v3
Checksum:iC904715DC0386056
GO
Isoform 2 (identifier: Q63810-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.

Note: No experimental confirmation available.
Show »
Length:160
Mass (Da):18,208
Checksum:iF94B070F21E70466
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31N → S in AAB23171 (PubMed:1325794).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1010Missing in isoform 2. 1 PublicationVSP_024842

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S43864 mRNA. Translation: AAB23171.1.
AK133403 mRNA. Translation: BAE21637.1.
AK145541 mRNA. Translation: BAE26495.1.
AK160938 mRNA. Translation: BAE36102.1.
AK163241 mRNA. Translation: BAE37251.1.
AL713926, AL606466 Genomic DNA. Translation: CAI51912.1.
AL606466, AL713926 Genomic DNA. Translation: CAI51920.1.
CCDSiCCDS24448.1. [Q63810-1]
PIRiJC1220.
RefSeqiNP_077779.2. NM_024459.2. [Q63810-1]
UniGeneiMm.41840.

Genome annotation databases

EnsembliENSMUST00000102880; ENSMUSP00000099944; ENSMUSG00000033953. [Q63810-1]
GeneIDi19058.
KEGGimmu:19058.
UCSCiuc007ibz.1. mouse. [Q63810-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S43864 mRNA. Translation: AAB23171.1.
AK133403 mRNA. Translation: BAE21637.1.
AK145541 mRNA. Translation: BAE26495.1.
AK160938 mRNA. Translation: BAE36102.1.
AK163241 mRNA. Translation: BAE37251.1.
AL713926, AL606466 Genomic DNA. Translation: CAI51912.1.
AL606466, AL713926 Genomic DNA. Translation: CAI51920.1.
CCDSiCCDS24448.1. [Q63810-1]
PIRiJC1220.
RefSeqiNP_077779.2. NM_024459.2. [Q63810-1]
UniGeneiMm.41840.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ORBX-ray3.11B1-170[»]
ProteinModelPortaliQ63810.
SMRiQ63810. Positions 7-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202347. 1 interaction.
IntActiQ63810. 3 interactions.
MINTiMINT-1513271.
STRINGi10090.ENSMUSP00000099944.

PTM databases

iPTMnetiQ63810.
PhosphoSiteiQ63810.

Proteomic databases

EPDiQ63810.
MaxQBiQ63810.
PaxDbiQ63810.
PRIDEiQ63810.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102880; ENSMUSP00000099944; ENSMUSG00000033953. [Q63810-1]
GeneIDi19058.
KEGGimmu:19058.
UCSCiuc007ibz.1. mouse. [Q63810-1]

Organism-specific databases

CTDi5534.
MGIiMGI:107172. Ppp3r1.

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119179.
HOGENOMiHOG000233019.
HOVERGENiHBG105307.
InParanoidiQ63810.
KOiK06268.
OMAiYIGNGEL.
OrthoDBiEOG75QR5D.
PhylomeDBiQ63810.
TreeFamiTF105558.

Miscellaneous databases

ChiTaRSiPpp3r1. mouse.
PROiQ63810.
SOURCEiSearch...

Gene expression databases

BgeeiQ63810.
GenevisibleiQ63810. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B)."
    Ueki K., Muramatsu T., Kincaid R.L.
    Biochem. Biophys. Res. Commun. 187:537-543(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Egg and Head.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 58-85; 104-117; 126-135 AND 148-164, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain and Hippocampus.
  5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. Cited for: INTERACTION WITH CIB1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCANB1_MOUSE
AccessioniPrimary (citable) accession number: Q63810
Secondary accession number(s): Q3V067, Q5F225
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 2007
Last modified: June 8, 2016
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein has four functional calcium-binding sites.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.