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Protein

Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas

Gene

Gnas

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. XLas isoforms interact with the same set of receptors as Gnas isoforms.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi804 – 8041MagnesiumBy similarity
Metal bindingi954 – 9541MagnesiumBy similarity
Binding sitei1116 – 11161GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi797 – 8059GTPBy similarity
Nucleotide bindingi947 – 9548GTPBy similarity
Nucleotide bindingi973 – 9775GTPBy similarity
Nucleotide bindingi1042 – 10454GTPBy similarity

GO - Molecular functioni

  • alpha-tubulin binding Source: RGD
  • beta-2 adrenergic receptor binding Source: RGD
  • corticotropin-releasing hormone receptor 1 binding Source: RGD
  • D1 dopamine receptor binding Source: RGD
  • G-protein alpha-subunit binding Source: RGD
  • G-protein beta-subunit binding Source: RGD
  • GTPase activity Source: InterPro
  • GTP binding Source: RGD
  • insulin-like growth factor receptor binding Source: RGD
  • ionotropic glutamate receptor binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • mu-type opioid receptor binding Source: RGD
  • protein domain specific binding Source: RGD
  • signal transducer activity Source: RGD

GO - Biological processi

  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: RGD
  • calcium ion homeostasis Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • negative regulation of blood pressure Source: RGD
  • negative regulation of renal sodium excretion Source: RGD
  • positive regulation of cAMP biosynthetic process Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of sodium ion transport Source: RGD
  • regulation of transcription from RNA polymerase II promoter Source: RGD
  • sensory perception of chemical stimulus Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas
Alternative name(s):
Adenylate cyclase-stimulating G alpha protein
Extra large alphas protein
Short name:
XLalphas
G-alpha-8
Gene namesi
Name:GnasImported
Synonyms:Gnas1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2716. Gnas.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • endosome Source: RGD
  • heterotrimeric G-protein complex Source: RGD
  • membrane Source: RGD
  • membrane raft Source: RGD
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • ruffle Source: RGD
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi977 – 9771Q → L: Large increase in adenylyl cyclase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11441144Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLasPRO_0000253986Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei951 – 9511ADP-ribosylarginine; by cholera toxinBy similarity
Modified residuei1102 – 11021PhosphoserineCombined sources

Keywords - PTMi

ADP-ribosylation, Phosphoprotein

Proteomic databases

PRIDEiQ63803.

PTM databases

iPTMnetiQ63803.

Expressioni

Tissue specificityi

Enriched in neuroendocrine tissues with a particularly high level of expression in pituitary where it is abundant in intermediate and anterior lobes. In adrenal gland, found in central region containing medullary chromaffin cells but not in cortex. In cerebellum, strongly expressed in perikarya of Purkinje cells. Not detected in liver, kidney or neurohypophysis.2 Publications

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts through its N-terminal region with ALEX which is produced from the same locus in a different open reading frame. This interaction may inhibit its adenylyl cyclase-stimulating activity.2 Publications

GO - Molecular functioni

  • alpha-tubulin binding Source: RGD
  • beta-2 adrenergic receptor binding Source: RGD
  • corticotropin-releasing hormone receptor 1 binding Source: RGD
  • D1 dopamine receptor binding Source: RGD
  • G-protein alpha-subunit binding Source: RGD
  • G-protein beta-subunit binding Source: RGD
  • insulin-like growth factor receptor binding Source: RGD
  • ionotropic glutamate receptor binding Source: RGD
  • mu-type opioid receptor binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

IntActiQ63803. 1 interaction.
MINTiMINT-4569853.

Structurei

3D structure databases

ProteinModelPortaliQ63803.
SMRiQ63803. Positions 787-1141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili745 – 77228Sequence analysisAdd
BLAST
Coiled coili837 – 86327Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi96 – 272177Pro-richSequence analysisAdd
BLAST
Compositional biasi454 – 630177Ala-richSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the G-alpha family. G(s) subfamily.Sequence analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOVERGENiHBG079975.
InParanoidiQ63803.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 2 hits.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform XLas-1 (identifier: Q63803-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGMLNCLHGN NMSGQHDIPP EVGDQPEQEP LEAQGAAAPG AGVGPAEEME
60 70 80 90 100
TEPSNNEPIP DETDSEVCGP PEDSKSDIQS PSQAFEEVQV GGDYSPPPEE
110 120 130 140 150
AMPFEIQQPS LGDFWPTLEQ PGPSGTPSGI KAFNPAILEP GTPTGAHPGL
160 170 180 190 200
GAYSPPPEEA MPFEFNEPAQ EDRCQPPLQV PDLAPGGPEA WVSRALPAEP
210 220 230 240 250
GNLGFENTGF REDYSPPPEE SVPFQLDGEE FGGDSPPPGL PRVTPQIGIG
260 270 280 290 300
GEFPTVAVPS TLCLAPAANA PPLWVQGAIG RPFREAVRSP NFAYDISPME
310 320 330 340 350
ITRPLLEIGR ASTGVDDDTA VNMDSPPIAS DGPPIEVSGA PVKSEHAKRP
360 370 380 390 400
PLERQAAETG NSPISSTTAE EAKVPSLERG EGSPTQPETV HIKPAPVAES
410 420 430 440 450
GTDSSKADPD SATHAVLQIG PEEVGGVPTM PTDLPPASED AGPDVRAEPD
460 470 480 490 500
GGTAPATPAE SEDNREPAAA AAAEPAAEPA AEPAAEPAAE PAAEPAAEAV
510 520 530 540 550
PDTEAESASG AVPDTQEEPA AAAASATPAE PAARAAPVTP TEPATRAVPS
560 570 580 590 600
ARAHPAAGAV PGASAMSAAA RAAAARAAYA GPLVWGARSL SATPAARASL
610 620 630 640 650
PARAAAAARA ASAARAVAAG RSASAAPSRA HLRPPSPEIQ VADPPTPRPA
660 670 680 690 700
PRPSAWPDKY ERGRSCCRYE AASGICEIES SSDESEEGAT GCFQWLLRRN
710 720 730 740 750
RRPGQPRSHT VGSNPVRNFF ARAFGSCFGL SECTRSRSLS PGKAKDPMEE
760 770 780 790 800
RRKQMRKEAM EMREQKRADK KRSKLIDKQL EEEKMDYMCT HRLLLLGAGE
810 820 830 840 850
SGKSTIVKQM RILHVNGFNG EGGEEDPQAA RSNSDGEKAT KVQDIKNNLK
860 870 880 890 900
EAIETIVAAM SNLVPPVELA NPENQFRVDY ILSVMNVPNF DFPPEFYEHA
910 920 930 940 950
KALWEDEGVR ACYERSNEYQ LIDCAQYFLD KIDVIKQADY VPSDQDLPRC
960 970 980 990 1000
RVLTSGIFET KFQVDKVNFH MFDVGGQRDE RRKWIQCFND VTAIIFVVAS
1010 1020 1030 1040 1050
SSYNMVIRED NQTNRLQEAL NLFKSIWNNR WLRTISVILF LNKQDLLAEK
1060 1070 1080 1090 1100
VLAGKSKIED YFPEFARYTT PEDATPEPGE DPRVTRAKYF IRDEFLRIST
1110 1120 1130 1140
ASGDGRHYCY PHFTCAVDTE NIRRVFNDCR DIIQRMHLRQ YELL
Length:1,144
Mass (Da):122,887
Last modified:October 17, 2006 - v3
Checksum:i8B151D85E76ABFA1
GO
Isoform Gnas-1Curated (identifier: P63095-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P63095.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:394
Mass (Da):45,664
GO
Isoform Gnas-2Curated (identifier: P63095-2) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P63095.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:362
Mass (Da):41,818
GO
Isoform Gnas-3Curated (identifier: P63095-3) [UniParc]FASTAAdd to basket
Also known as: GsaN1Curated
The sequence of this isoform can be found in the external entry P63095.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:91
Mass (Da):10,184
GO
Isoform Nesp55Curated (identifier: Q792G6-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q792G6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Shares no sequence similarity with other isoforms due to a novel first exon containing the entire reading frame spliced to shared exon 2 so that exons 2-13 make up the 3'-UTR.
Length:256
Mass (Da):29,219
GO

Sequence cautioni

The sequence CAC39211 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03134399 Genomic DNA. No translation available.
AABR03134465 Genomic DNA. No translation available.
X84047 mRNA. Translation: CAC39211.1. Different initiation.
UniGeneiRn.127731.
Rn.31.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03134399 Genomic DNA. No translation available.
AABR03134465 Genomic DNA. No translation available.
X84047 mRNA. Translation: CAC39211.1. Different initiation.
UniGeneiRn.127731.
Rn.31.

3D structure databases

ProteinModelPortaliQ63803.
SMRiQ63803. Positions 787-1141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63803. 1 interaction.
MINTiMINT-4569853.

PTM databases

iPTMnetiQ63803.

Proteomic databases

PRIDEiQ63803.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi2716. Gnas.

Phylogenomic databases

HOVERGENiHBG079975.
InParanoidiQ63803.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 2 hits.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGNAS1_RAT
AccessioniPrimary (citable) accession number: Q63803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 17, 2006
Last modified: September 7, 2016
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is produced by a bicistronic gene which also produces the ALEX protein from an overlapping reading frame.1 Publication
The GNAS locus is imprinted in a complex manner, giving rise to distinct paternally, maternally and biallelically expressed proteins. The XLas isoforms are paternally derived, the Gnas isoforms are biallelically derived and the Nesp55 isoforms are maternally derived.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.