Q63802 (WEE1_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified April 16, 2014. Version 112. History...
Names and origin
|Protein names||Recommended name:|
Wee1-like protein kinase
|Organism||Rattus norvegicus (Rat) [Reference proteome]|
|Taxonomic identifier||10116 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus|
|Sequence length||646 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation By similarity.
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Binds 2 magnesium ions per subunit By similarity.
Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location By similarity.
Binds to 14-3-3 protein zeta.
Nucleus By similarity.
Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-52 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase By similarity.
Dephosphorylated at Thr-239 by CTDP1 By similarity.
Ubiquitinated and degraded at the onset of G2/M phase By similarity.
Contains 1 protein kinase domain.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 646||646||Wee1-like protein kinase||PRO_0000086812|
|Domain||298 – 568||271||Protein kinase|
|Nucleotide binding||304 – 312||9||ATP By similarity|
|Compositional bias||34 – 42||9||Poly-Glu|
|Compositional bias||74 – 77||4||Poly-Arg|
|Compositional bias||97 – 101||5||Poly-Gly|
|Active site||425||1||Proton acceptor By similarity|
|Metal binding||430||1||Magnesium; via carbonyl oxygen By similarity|
|Metal binding||462||1||Magnesium; via carbonyl oxygen By similarity|
|Binding site||327||1||ATP By similarity|
Amino acid modifications
|Modified residue||52||1||Phosphoserine; by PLK1 By similarity|
|Modified residue||123||1||Phosphoserine; by CDK1 By similarity|
|Modified residue||127||1||Phosphoserine By similarity|
|Modified residue||150||1||Phosphoserine By similarity|
|Modified residue||190||1||Phosphothreonine By similarity|
|Modified residue||239||1||Phosphothreonine By similarity|
|Modified residue||311||1||Phosphoserine By similarity|
|Modified residue||642||1||Phosphoserine; by BRSK1 and BRSK2 By similarity|
Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."|
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
|D31838 mRNA. Translation: BAA06624.1.|
BC090346 mRNA. Translation: AAH90346.1.
|RefSeq||NP_001012760.1. NM_001012742.1. |
3D structure databases
|SMR||Q63802. Positions 290-568. |
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSRNOT00000013362; ENSRNOP00000013362; ENSRNOG00000010017. |
|UCSC||RGD:1307895. rat. |
|RGD||1307895. Wee1. |
Enzyme and pathway databases
|BRENDA||18.104.22.168. 5301. |
Gene expression databases
Family and domain databases
|InterPro||IPR011009. Kinase-like_dom. |
|Pfam||PF00069. Pkinase. 1 hit. |
|PIRSF||PIRSF037281. Wee1-like_protein_kinase. 1 hit. |
|SUPFAM||SSF56112. SSF56112. 1 hit. |
|PROSITE||PS00107. PROTEIN_KINASE_ATP. 1 hit. |
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
|Accession||Primary (citable) accession number: Q63802|
Secondary accession number(s): Q5EAN3
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families