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Q63802

- WEE1_RAT

UniProt

Q63802 - WEE1_RAT

Protein

Wee1-like protein kinase

Gene

Wee1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 2 magnesium ions per subunit.By similarity

    Enzyme regulationi

    Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei327 – 3271ATPPROSITE-ProRule annotation
    Active sitei425 – 4251Proton acceptorPROSITE-ProRule annotation
    Metal bindingi430 – 4301Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi462 – 4621Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi304 – 3129ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein serine/threonine kinase activity Source: InterPro

    GO - Biological processi

    1. establishment of cell polarity Source: Ensembl
    2. microtubule cytoskeleton organization Source: Ensembl
    3. mitotic nuclear division Source: UniProtKB-KW
    4. neuron projection morphogenesis Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 5301.
    ReactomeiREACT_196232. Factors involved in megakaryocyte development and platelet production.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wee1-like protein kinase (EC:2.7.10.2)
    Alternative name(s):
    Wee1A kinase
    Gene namesi
    Name:Wee1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi1307895. Wee1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 646646Wee1-like protein kinasePRO_0000086812Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei52 – 521Phosphoserine; by PLK1By similarity
    Modified residuei123 – 1231Phosphoserine; by CDK1By similarity
    Modified residuei127 – 1271PhosphoserineBy similarity
    Modified residuei139 – 1391Phosphoserine
    Modified residuei150 – 1501PhosphoserineBy similarity
    Modified residuei190 – 1901PhosphothreonineBy similarity
    Modified residuei239 – 2391PhosphothreonineBy similarity
    Modified residuei311 – 3111PhosphoserineBy similarity
    Modified residuei642 – 6421Phosphoserine; by BRSK1 and BRSK2By similarity

    Post-translational modificationi

    Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-52 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase By similarity.By similarity
    Dephosphorylated at Thr-239 by CTDP1.By similarity
    Ubiquitinated and degraded at the onset of G2/M phase.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ63802.
    PRIDEiQ63802.

    PTM databases

    PhosphoSiteiQ63802.

    Expressioni

    Gene expression databases

    GenevestigatoriQ63802.

    Interactioni

    Subunit structurei

    Binds to 14-3-3 protein zeta.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63802.
    SMRiQ63802. Positions 290-568.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini298 – 568271Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi34 – 429Poly-Glu
    Compositional biasi74 – 774Poly-Arg
    Compositional biasi97 – 1015Poly-Gly

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000063230.
    HOGENOMiHOG000004824.
    HOVERGENiHBG005050.
    InParanoidiQ63802.
    KOiK06632.
    OMAiRTYWNDS.
    OrthoDBiEOG7N63M9.
    PhylomeDBiQ63802.
    TreeFamiTF101088.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q63802-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFLSRQQPP PTRRAAAACS LRQKLIFSPG SDCEEEEEEE EEGSGHSTGE    50
    DSAFQEPDSP LPSARSPAEA EAERRRRSPG AEPSSPGELE EDLLLRGGGG 100
    GAQAAGGGAE GDSWEEEGFG SSSPVKSPTT AYFLGSSFSP VRCGGPGDAS 150
    PRGCGVPRAM DDPCSPQPDY PSTPPHKTFR KLRLFDTPHT PKSLLSKARV 200
    IDSSSVKLRG SSLFMDTEKS GKREFDTRQT PQVNINPFTP DPVMLHSSGQ 250
    CRGRKRAYFN DSSEDMEASD YEFEDETRPA KRITITESNM KSRYTTEFHE 300
    LEKIGSGEFG SVFKCVKRLD GCIYAIKRSK KPLAGSVDEQ NALREVYAHA 350
    VLGQHPHVVR YFSAWAEDDH MLIQNEYCNG GSLADAVSEN YRVMSYFTEA 400
    ELKDLLLQVG RGLRYIHSMS LVHMDIKPSN IFISRTSIPN AVSEEGDEDD 450
    WISNKVMFKI GDLGHVTRIS SPQVEEGDSR FLANEVLQEN YSHLPKADIF 500
    ALALTVVCAA GAEPLPRNGD QWHEIRQGRL PRIPQVLSQE LTELLKVMIH 550
    PDPERRPSAM VLVKHSVLLS ASRKSAEQLR IELNAEKFKN SLLQKELKKA 600
    QMAAKVAAEE RALLTDRMAT RSTTQSNRTS RLIGKKMNRS VSLTIY 646
    Length:646
    Mass (Da):71,496
    Last modified:November 1, 1996 - v1
    Checksum:i740120F51C811DAF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31838 mRNA. Translation: BAA06624.1.
    BC090346 mRNA. Translation: AAH90346.1.
    RefSeqiNP_001012760.1. NM_001012742.1.
    UniGeneiRn.208255.

    Genome annotation databases

    EnsembliENSRNOT00000013362; ENSRNOP00000013362; ENSRNOG00000010017.
    GeneIDi308937.
    KEGGirno:308937.
    UCSCiRGD:1307895. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31838 mRNA. Translation: BAA06624.1 .
    BC090346 mRNA. Translation: AAH90346.1 .
    RefSeqi NP_001012760.1. NM_001012742.1.
    UniGenei Rn.208255.

    3D structure databases

    ProteinModelPortali Q63802.
    SMRi Q63802. Positions 290-568.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q63802.

    Proteomic databases

    PaxDbi Q63802.
    PRIDEi Q63802.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000013362 ; ENSRNOP00000013362 ; ENSRNOG00000010017 .
    GeneIDi 308937.
    KEGGi rno:308937.
    UCSCi RGD:1307895. rat.

    Organism-specific databases

    CTDi 7465.
    RGDi 1307895. Wee1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000063230.
    HOGENOMi HOG000004824.
    HOVERGENi HBG005050.
    InParanoidi Q63802.
    KOi K06632.
    OMAi RTYWNDS.
    OrthoDBi EOG7N63M9.
    PhylomeDBi Q63802.
    TreeFami TF101088.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 5301.
    Reactomei REACT_196232. Factors involved in megakaryocyte development and platelet production.

    Miscellaneous databases

    NextBioi 659827.
    PROi Q63802.

    Gene expression databases

    Genevestigatori Q63802.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Furunobu A.
      Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiWEE1_RAT
    AccessioniPrimary (citable) accession number: Q63802
    Secondary accession number(s): Q5EAN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 13, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3