Q63799 (TCEA2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription elongation factor A protein 2

Alternative name(s):
Protein S-II-T1
Testis-specific S-II
Transcription elongation factor S-II protein 2
Transcription elongation factor TFIIS.l

Gene names

Name:	Tcea2
Synonyms:	Siit1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Ref.1
Subunit structure	Interacts with the basal transcription factor GTF2B. Interacts with REXO1 By similarity.
Subcellular location	Nucleus.
Tissue specificity	Testis specific. Ref.1
Sequence similarities	Belongs to the TFS-II family. Contains 1 TFIIS central domain. Contains 1 TFIIS N-terminal domain. Contains 1 TFIIS-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: RGD regulation of DNA-dependent transcription, elongation Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	centrosome Inferred from electronic annotation. Source: Compara nucleus Traceable author statement Ref.1. Source: RGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Transcription elongation factor A protein 2		PRO_0000121451
Regions
	Domain	5 – 82	78	TFIIS N-terminal
	Domain	138 – 254	117	TFIIS central
	Zinc finger	257 – 297	41	TFIIS-type
Amino acid modifications
	Cross-link	57		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63799 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 24BF8D56ECE3BB3F	FASTA	299	33,528
	10 20 30 40 50 60 MGKEEEIARI ARRLDKMVTR KNAEGAMDLL RELKNMPITL HLLQSTRVGM SVNALRKQSS 70 80 90 100 110 120 DEELIALAKS LIKSWKKLLD VSDGKSRDQG RGTPLPTSSS KDASGTTDLS CKKPDPPRTS 130 140 150 160 170 180 STPRITTFPQ VPITCDAVRN KCREMLTLAL QTDHDHVAVG VSCEHLSSQI EECIFLDVGN 190 200 210 220 230 240 TDMKYKNRVR SRISNLKDAK NPGLRRNVLC GAITPQQIAV MTSEEMASDE LKEIRKAMTK 250 260 270 280 290 EAIREHQMAR TGGTQTDLFT CNKCRKKNCT YTQVQTRSSD EPMTTYVVCN ECGNRWKFC

References

[1]	"Cloning and identification of testis-specific transcription elongation factor S-II." Xu Q., Nakanishi T., Sekimizu K., Natori S. J. Biol. Chem. 269:3100-3103(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12927 mRNA. Translation: BAA02310.1.
IPI	IPI00339171.
PIR	A53004.
RefSeq	NP_476439.1. NM_057098.1.
UniGene	Rn.78891.
3D structure databases
ProteinModelPortal	Q63799.
SMR	Q63799. Positions 1-83, 250-299.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000059673.
Proteomic databases
PaxDb	Q63799.
PRIDE	Q63799.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000022073; ENSRNOP00000022073; ENSRNOG00000016288.
GeneID	29575.
KEGG	rno:29575.
UCSC	RGD:61825. rat.
Organism-specific databases
CTD	6919.
RGD	61825. Tcea2.
Phylogenomic databases
eggNOG	COG1594.
GeneTree	ENSGT00390000017794.
HOGENOM	HOG000195015.
HOVERGEN	HBG055022.
InParanoid	Q63799.
KO	K03145.
OrthoDB	EOG4SN1PH.
Gene expression databases
ArrayExpress	Q63799.
Genevestigator	Q63799.
GermOnline	ENSRNOG00000016288. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.472.30. 1 hit. 1.20.930.10. 1 hit.
InterPro	IPR016492. TF_IIS-rel. IPR003617. TFIIS/CRSP70_N_sub. IPR003618. TFIIS_cen_dom. IPR017923. TFIIS_N. IPR017890. TFS2M. IPR006289. TFSII. IPR001222. Znf_TFIIS. [Graphical view]
Pfam	PF08711. Med26. 1 hit. PF01096. TFIIS_C. 1 hit. PF07500. TFIIS_M. 1 hit. [Graphical view]
PIRSF	PIRSF006704. TF_IIS. 1 hit.
SMART	SM00510. TFS2M. 1 hit. SM00509. TFS2N. 1 hit. SM00440. ZnF_C2C2. 1 hit. [Graphical view]
SUPFAM	SSF46942. TFIIS_centre. 1 hit. SSF47676. TFIIS_conserved. 1 hit.
TIGRFAMs	TIGR01385. TFSII. 1 hit.
PROSITE	PS51321. TFIIS_CENTRAL. 1 hit. PS51319. TFIIS_N. 1 hit. PS00466. ZF_TFIIS_1. 1 hit. PS51133. ZF_TFIIS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	609666.

Entry information

Entry name

TCEA2_RAT

Accession

Primary (citable) accession number: Q63799

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program