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Q63796 (M3K12_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase kinase kinase 12
EC=2.7.11.25
Alternative name(s):
Dual leucine zipper bearing kinase
Short name=DLK
Leucine-zipper protein kinase
Short name=ZPK
MAPK-upstream kinase
Short name=MUK
Mixed lineage kinase
Gene names
Name:Map3k12
Synonyms:Muk
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length888 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Subunit structure

Interacts with MBIP By similarity.

Subcellular location

Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.

Domain

Interacts with MBIP through the leucine-zipper motif By similarity.

Post-translational modification

Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 888888Mitogen-activated protein kinase kinase kinase 12
PRO_0000086263

Regions

Domain158 – 399242Protein kinase
Domain423 – 44422Leucine-zipper 1
Domain476 – 49722Leucine-zipper 2
Nucleotide binding164 – 1729ATP By similarity
Compositional bias56 – 627Poly-Gly
Compositional bias668 – 6714Poly-Pro
Compositional bias698 – 7014Poly-Pro
Compositional bias753 – 76412Glu-rich (acidic)

Sites

Active site2691Proton acceptor By similarity
Binding site1851ATP By similarity

Amino acid modifications

Modified residue6671Phosphoserine By similarity
Modified residue7901Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q63796 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 52AD964006BAE149

FASTA88896,308
        10         20         30         40         50         60 
MACLHETRTP SPSFGGFVST LSEASMRKLD PDTSDCTPEK DLTPTQCVLR DVVPLGGQGG 

        70         80         90        100        110        120 
GGPSPSPGGE PPPEPFANSV LQLHEQDTGG PGGATGSPES RASRVRADEV RLQCQSGSGF 

       130        140        150        160        170        180 
LEGLFGCLRP VWTMIGKAYS TEHKQQQEDL WEVPFEEILD LQWVGSGAQG AVFLGRFHGE 

       190        200        210        220        230        240 
EVAVKKVRDL KETDIKHLRK LKHPNIITFK GVCTQAPCYC ILMEFCAQGQ LYEVLRAGRP 

       250        260        270        280        290        300 
VTPSLLVDWS MGIAGGMNYL HLHKIIHRDL KSPNMLITYD DVVKISDFGT SKELSDKSTK 

       310        320        330        340        350        360 
MSFAGTVAWM APEVIRNEPV SEKVDIWSFG VVLWELLTGE IPYKDVDSSA IIWGVGSNSL 

       370        380        390        400        410        420 
HLPVPSSCPD GFKILLRQCW NRKPRNRPSF RQILLHLDIA SADVLSTPQE TYFKSQAEWR 

       430        440        450        460        470        480 
EEVKLHFEKI KSEGTCLHRL EEELVMRRRE ELRHALDIRE HYERKLERAN NLYMELNALM 

       490        500        510        520        530        540 
LQLELKEREL LRREQALERR CPGLLKSHTS RSLLHGNTME KLIKKRNVPQ KLSPHSKRPD 

       550        560        570        580        590        600 
ILKTESLLPK LDAALSGVGL PGCPKAPPSP GRSRRGKTRH RKASAKGSCG DLPGLRAALP 

       610        620        630        640        650        660 
PHEPGGLGSP GGLGVGPTAW DASPPALRGL HHDLLLRKMS SSSPDLLSAA LGARGRGATG 

       670        680        690        700        710        720 
GARDPGSPPP PQGDTPPSEG SAPGSTSPDS PGGAKGEPPP PVGPGEGVGL LGTGREGTTG 

       730        740        750        760        770        780 
RGGSRAGYQH LTPAALLYRA AVTRSQKRGI SSEEEEGEVD SEVELPPSQR WPQGPNMRQS 

       790        800        810        820        830        840 
LSTFSSENPS DVEEGTASEP SPSGTPEVGS TNTDERPDER SDDMCSQGSE IPLDLPTSEV 

       850        860        870        880 
VPERETSSLP MQHQDDQGPN PEDSDCDSTE LDNSNSIDAL PPPASLPP

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References

[1]"Activation of the JNK pathway by distantly related protein kinases, MEKK and MUK."
Hirai S., Izawa M., Osada S., Spyrou G., Ohno S.
Oncogene 12:641-650(1996) [PubMed: 8637721] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Lubec G., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 440-448, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D49785 mRNA. Translation: BAA08621.1.
IPIIPI00211968.
PIRJC5399.
RefSeqNP_037187.1. NM_013055.1.
UniGeneRn.146082.

3D structure databases

ProteinModelPortalQ63796.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ63796.

Proteomic databases

PRIDEQ63796.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25579.
KEGGrno:25579.
UCSCNM_013055. rat.

Organism-specific databases

CTD7786.
RGD3988. Map3k12.

Phylogenomic databases

eggNOGmaNOG11674.
HOVERGENHBG052383.
InParanoidQ63796.
OrthoDBEOG4G4GPV.

Gene expression databases

ArrayExpressQ63796.
GenevestigatorQ63796.
GermOnlineENSRNOG00000015134. Rattus norvegicus.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR017419. MAP3K12_MAP3K13.
IPR000719. Prot_kinase_cat_dom.
IPR001245. Ser-Thr/Tyr_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
KOK04423.
PfamPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFPIRSF038165. MAPKKK12_MAPKKK13. 1 hit.
PRINTSPR00109. TYRKINASE.
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio607223.

Entry information

Entry nameM3K12_RAT
AccessionPrimary (citable) accession number: Q63796
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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