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Protein

Mitogen-activated protein kinase kinase kinase 12

Gene

Map3k12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei185 – 1851ATPPROSITE-ProRule annotation
Active sitei269 – 2691Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi164 – 1729ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 12 (EC:2.7.11.25)
Alternative name(s):
Dual leucine zipper bearing kinase
Short name:
DLK
Leucine-zipper protein kinase
Short name:
ZPK
MAPK-upstream kinase
Short name:
MUK
Mixed lineage kinase
Gene namesi
Name:Map3k12
Synonyms:Muk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3988. Map3k12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 888888Mitogen-activated protein kinase kinase kinase 12PRO_0000086263Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei37 – 371PhosphothreonineBy similarity
Modified residuei43 – 431PhosphothreonineBy similarity
Modified residuei640 – 6401PhosphoserineBy similarity

Post-translational modificationi

Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63796.
PRIDEiQ63796.

PTM databases

iPTMnetiQ63796.
SwissPalmiQ63796.

Interactioni

Subunit structurei

Interacts with MBIP.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020437.

Structurei

3D structure databases

ProteinModelPortaliQ63796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini158 – 399242Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni423 – 44422Leucine-zipper 1Add
BLAST
Regioni476 – 49722Leucine-zipper 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi56 – 627Poly-Gly
Compositional biasi668 – 6714Poly-Pro
Compositional biasi698 – 7014Poly-Pro
Compositional biasi753 – 76412Glu-rich (acidic)Add
BLAST

Domaini

Interacts with MBIP through the leucine-zipper motif.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4721. Eukaryota.
ENOG410YKX2. LUCA.
HOGENOMiHOG000113435.
HOVERGENiHBG052383.
InParanoidiQ63796.
KOiK04423.
PhylomeDBiQ63796.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR017419. MAP3K12_MAP3K13.
IPR027257. MAPKKK12.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFiPIRSF500741. MAPKKK12. 1 hit.
PIRSF038165. MAPKKK12_MAPKKK13. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACLHETRTP SPSFGGFVST LSEASMRKLD PDTSDCTPEK DLTPTQCVLR
60 70 80 90 100
DVVPLGGQGG GGPSPSPGGE PPPEPFANSV LQLHEQDTGG PGGATGSPES
110 120 130 140 150
RASRVRADEV RLQCQSGSGF LEGLFGCLRP VWTMIGKAYS TEHKQQQEDL
160 170 180 190 200
WEVPFEEILD LQWVGSGAQG AVFLGRFHGE EVAVKKVRDL KETDIKHLRK
210 220 230 240 250
LKHPNIITFK GVCTQAPCYC ILMEFCAQGQ LYEVLRAGRP VTPSLLVDWS
260 270 280 290 300
MGIAGGMNYL HLHKIIHRDL KSPNMLITYD DVVKISDFGT SKELSDKSTK
310 320 330 340 350
MSFAGTVAWM APEVIRNEPV SEKVDIWSFG VVLWELLTGE IPYKDVDSSA
360 370 380 390 400
IIWGVGSNSL HLPVPSSCPD GFKILLRQCW NRKPRNRPSF RQILLHLDIA
410 420 430 440 450
SADVLSTPQE TYFKSQAEWR EEVKLHFEKI KSEGTCLHRL EEELVMRRRE
460 470 480 490 500
ELRHALDIRE HYERKLERAN NLYMELNALM LQLELKEREL LRREQALERR
510 520 530 540 550
CPGLLKSHTS RSLLHGNTME KLIKKRNVPQ KLSPHSKRPD ILKTESLLPK
560 570 580 590 600
LDAALSGVGL PGCPKAPPSP GRSRRGKTRH RKASAKGSCG DLPGLRAALP
610 620 630 640 650
PHEPGGLGSP GGLGVGPTAW DASPPALRGL HHDLLLRKMS SSSPDLLSAA
660 670 680 690 700
LGARGRGATG GARDPGSPPP PQGDTPPSEG SAPGSTSPDS PGGAKGEPPP
710 720 730 740 750
PVGPGEGVGL LGTGREGTTG RGGSRAGYQH LTPAALLYRA AVTRSQKRGI
760 770 780 790 800
SSEEEEGEVD SEVELPPSQR WPQGPNMRQS LSTFSSENPS DVEEGTASEP
810 820 830 840 850
SPSGTPEVGS TNTDERPDER SDDMCSQGSE IPLDLPTSEV VPERETSSLP
860 870 880
MQHQDDQGPN PEDSDCDSTE LDNSNSIDAL PPPASLPP
Length:888
Mass (Da):96,308
Last modified:November 1, 1996 - v1
Checksum:i52AD964006BAE149
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49785 mRNA. Translation: BAA08621.1.
PIRiJC5399.
RefSeqiNP_037187.1. NM_013055.1.
UniGeneiRn.146082.

Genome annotation databases

GeneIDi25579.
KEGGirno:25579.
UCSCiRGD:3988. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49785 mRNA. Translation: BAA08621.1.
PIRiJC5399.
RefSeqiNP_037187.1. NM_013055.1.
UniGeneiRn.146082.

3D structure databases

ProteinModelPortaliQ63796.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020437.

PTM databases

iPTMnetiQ63796.
SwissPalmiQ63796.

Proteomic databases

PaxDbiQ63796.
PRIDEiQ63796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25579.
KEGGirno:25579.
UCSCiRGD:3988. rat.

Organism-specific databases

CTDi7786.
RGDi3988. Map3k12.

Phylogenomic databases

eggNOGiKOG4721. Eukaryota.
ENOG410YKX2. LUCA.
HOGENOMiHOG000113435.
HOVERGENiHBG052383.
InParanoidiQ63796.
KOiK04423.
PhylomeDBiQ63796.

Miscellaneous databases

NextBioi607223.
PROiQ63796.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR017419. MAP3K12_MAP3K13.
IPR027257. MAPKKK12.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFiPIRSF500741. MAPKKK12. 1 hit.
PIRSF038165. MAPKKK12_MAPKKK13. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Activation of the JNK pathway by distantly related protein kinases, MEKK and MUK."
    Hirai S., Izawa M., Osada S., Spyrou G., Ohno S.
    Oncogene 12:641-650(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 440-448, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiM3K12_RAT
AccessioniPrimary (citable) accession number: Q63796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.