Reviewed, UniProtKB/Swiss-Prot Q63789 (P55G_RAT)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 3-kinase regulatory subunit gamma
Alternative name(s):
    PI3-kinase p85 subunit gamma
    PtdIns-3-kinase p85-gamma
    p55PIK

Gene names

Name:

Pik3r3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
Subunit structure	Heterodimer of a p110 (catalytic) and a p55 (regulatory) subunits.
Tissue specificity	Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle. Barely detectable in liver and spleen.
Sequence similarities	Belongs to the PI3K p85 subunit family. Contains 2 SH2 domains.

Ontologies

Keywords
Domain	Repeat SH2 domain
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular function	1-phosphatidylinositol-3-kinase regulator activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Phosphatidylinositol 3-kinase regulatory subunit gamma		PRO_0000080769
Regions
	Domain	65 – 163	99	SH2 1
	Domain	358 – 455	98	SH2 2
Amino acid modifications
	Modified residue	199	1	Phosphotyrosine By similarity
	Modified residue	341	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63789-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6467D88447CC562B	FASTA	461	54,254
	10 20 30 40 50 60 MYNTVWSMDR DDADWREVMM PYSTELIFYI EMDPPALPPK PPKPVTSAVT NGMKDCFVSL 70 80 90 100 110 120 QDAEWYWGDI SREEVNDKLR DMPDGTFLVR DASTKMQGDY TLTLRKGGNN KLIKIYHRDG 130 140 150 160 170 180 NYGFSEPLTF NSVVELINHY HHESLAQYNP KLDVKLMYPV SRYQQDQLVK EDNIDAVGKN 190 200 210 220 230 240 LQEFHSQYQE KSKEYDRLYE EYTRTSQEIQ MKRTAIEAFN ETIKIFEEQC HTQEQHSKDY 250 260 270 280 290 300 IERFRREGNE KEIERIMMNY DKLKSRLGEI HDSKVRLEQD LKKQALDNRE IDKKMNSIKP 310 320 330 340 350 360 DLIQLRKIRD QHLVWLNHRG VRQRRLNAWL GIKSEDTDES YFINEDDESL PHYDEKTWFV 370 380 390 400 410 420 EDVNRVQAED LLYGKPDGAF LIRESSKKGC YACSVVADGE VKPCVIYSPA RGYGFAEPYN 430 440 450 460 LYGSLKELVL HYQQTSLVQH NDSLNVTLAY PVHAQMPSLC R

References

[1]

"A novel 55-kDa regulatory subunit for phosphatidylinositol 3-kinase structurally similar to p55PIK is generated by alternative splicing of the p85alpha gene." Inukai K., Anai M., van Breda E., Hosaka T., Katagiri H., Funaki M., Fukushima Y., Ogihara T., Yazaki Y., Kikuchi M., Oka Y., Asano T. J. Biol. Chem. 271:5317-5320(1996) [PubMed: 8621382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D64047 mRNA. Translation: BAA10927.1.
IPI	IPI00211948.
RefSeq	NP_071549.1.
UniGene	Rn.44448
3D structure databases
HSSP	HSSP built from PDB template 2PNB based on UniProtKB P23727.
SMR	Q63789. Positions 58-171, 350-454.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63789.
Genome annotation databases
Ensembl	ENSRNOT00000000157; ENSRNOP00000000157; ENSRNOG00000000145; Rattus norvegicus. [Genome view]
GeneID	60664.
KEGG	rno:60664.
Organism-specific databases
CTD	60664.
RGD	621042. Pik3r3.
Phylogenomic databases
HOVERGEN	Q63789.
Gene expression databases
ArrayExpress	Q63789.
Genevestigator	Q63789.
GermOnline	ENSRNOG00000000145. Rattus norvegicus.
Family and domain databases
InterPro	IPR001720. PI3kinase_P85. IPR000980. SH2. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 2 hits.
PANTHER	PTHR10155. PI3kinase_P85. 1 hit.
Pfam	PF00017. SH2. 2 hits. [Graphical view]
PRINTS	PR00678. PI3KINASEP85. PR00401. SH2DOMAIN.
ProDom	PD000093. SH2. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00252. SH2. 2 hits. [Graphical view]
PROSITE	PS50001. SH2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	612373.

Entry information

Entry name

P55G_RAT

Accession

Primary (citable) accession number: Q63789

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	November 1, 1996
Last modified:	October 13, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)