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Q63788 (P85B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphatidylinositol 3-kinase regulatory subunit beta
Short name=PI3-kinase regulatory subunit beta
Short name=PI3K regulatory subunit beta
Short name=PtdIns-3-kinase regulatory subunit beta
Alternative name(s):
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
Short name=PI3-kinase subunit p85-beta
Short name=PtdIns-3-kinase regulatory subunit p85-beta
Gene names
Name:Pik3r2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length722 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

Subunit structure

Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated). Interacts with NYAP1, NYAP2 and MYO16 By similarity.

Post-translational modification

Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ By similarity.

Sequence similarities

Belongs to the PI3K p85 subunit family.

Contains 1 Rho-GAP domain.

Contains 2 SH2 domains.

Contains 1 SH3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 722722Phosphatidylinositol 3-kinase regulatory subunit beta
PRO_0000080765

Regions

Domain4 – 8077SH3
Domain112 – 289178Rho-GAP
Domain324 – 41996SH2 1
Domain616 – 71095SH2 2

Amino acid modifications

Modified residue4581Phosphotyrosine By similarity
Modified residue5991Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q63788 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 1208368B9F6F0C95

FASTA72281,329
        10         20         30         40         50         60 
MAGAEGFQYR AVYPFRRERP EDLELLPGDL LVVSRVALQA LGVADGGERC PHNVGWMPGF 

        70         80         90        100        110        120 
NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP LDGPSESGHT LASLAEQFSP 

       130        140        150        160        170        180 
PESAPPILVK LIEAIEQAEL DSEFYSRPEL PAPRTDWSLS DLEQWDRTTL YDAVKGFLLA 

       190        200        210        220        230        240 
LPAAVVTPEA ASEAYRAMRE VTGPVGLVLE PPTLPLHQAL TLRFLLQHLG RVARRAPSPA 

       250        260        270        280        290        300 
TAVHALASAF GPLLLRAPPP GGEGDGSEPA PDFPVLLLER LVQEHVDEQD TAPPALPPKP 

       310        320        330        340        350        360 
SKVKPAPTAL ANGGSTPSLQ DAEWYWGDIS REEVNERLRD TPDGTFLVRD ASSKIQGEYT 

       370        380        390        400        410        420 
LTLRKGGNNK LIKVFHRDGH YGFSEPLTFC SVVELISHYR HESLAQYNAK LDTRLLYPVS 

       430        440        450        460        470        480 
KYQQDQVVKE DSVEAVGAQL KVYHQQYQDK SREYDQLYEE YTRTSQELQM KRTAIEAFNE 

       490        500        510        520        530        540 
TILIFEEQGQ TQEKCSKEYL ERFRREGNEK EMQRILLNSE RLKSRIAEIH ESRTKLEQDL 

       550        560        570        580        590        600 
RAQASDNREI DKRMNSLKPD LMQLRKIRDQ YLVWLTQKGA RQRKINEWLG IKNETEDQYS 

       610        620        630        640        650        660 
LMEDEDALPH HEERTWYVGK INRTQAEEML SAKRDGTFLI RESSQRGCYA CSVVVDGDTK 

       670        680        690        700        710        720 
HCVIYRTATG FGFAEPYNLY GSLKELVLHY QHASLVQHND ALTVTLAHPV RAPGPGPPPA 


AR

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References

[1]"A novel 55-kDa regulatory subunit for phosphatidylinositol 3-kinase structurally similar to p55PIK is generated by alternative splicing of the p85alpha gene."
Inukai K., Anai M., van Breda E., Hosaka T., Katagiri H., Funaki M., Fukushima Y., Ogihara T., Yazaki Y., Kikuchi M., Oka Y., Asano T.
J. Biol. Chem. 271:5317-5320(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D64046 mRNA. Translation: BAA10926.1.
IPIIPI00211947.
UniGeneRn.22497.

3D structure databases

ProteinModelPortalQ63788.
SMRQ63788. Positions 4-86, 318-590, 608-712.
ModBaseSearch...

PTM databases

PhosphoSiteQ63788.

Proteomic databases

PaxDbQ63788.
PRIDEQ63788.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD68341. Pik3r2.

Phylogenomic databases

eggNOGNOG263689.
HOGENOMHOG000008438.
HOVERGENHBG082100.
InParanoidQ63788.
OrthoDBEOG49GKG4.

Enzyme and pathway databases

ReactomeREACT_114732. Cell-Cell communication.

Gene expression databases

ArrayExpressQ63788.
GenevestigatorQ63788.
GermOnlineENSRNOG00000019228. Rattus norvegicus.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
3.30.505.10. 2 hits.
InterProIPR001720. PI3kinase_P85.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR10155. PTHR10155. 1 hit.
PfamPF00620. RhoGAP. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]
PRINTSPR00678. PI3KINASEP85.
PR00401. SH2DOMAIN.
SMARTSM00324. RhoGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48350. Rho_GAP. 1 hit.
SSF50044. SH3. 1 hit.
PROSITEPS50238. RHOGAP. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP85B_RAT
AccessionPrimary (citable) accession number: Q63788
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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