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Q63788

- P85B_RAT

UniProt

Q63788 - P85B_RAT

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Protein

Phosphatidylinositol 3-kinase regulatory subunit beta

Gene

Pik3r2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy.By similarity

GO - Molecular functioni

  1. phosphatidylinositol 3-kinase regulator activity Source: InterPro

GO - Biological processi

  1. phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
  2. signal transduction Source: InterPro
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_258050. Nephrin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase regulatory subunit beta
Short name:
PI3-kinase regulatory subunit beta
Short name:
PI3K regulatory subunit beta
Short name:
PtdIns-3-kinase regulatory subunit beta
Alternative name(s):
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
Short name:
PI3-kinase subunit p85-beta
Short name:
PtdIns-3-kinase regulatory subunit p85-beta
Gene namesi
Name:Pik3r2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi68341. Pik3r2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. phosphatidylinositol 3-kinase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 722722Phosphatidylinositol 3-kinase regulatory subunit betaPRO_0000080765Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei458 – 4581PhosphotyrosineBy similarity
Modified residuei599 – 5991PhosphotyrosineBy similarity
Modified residuei649 – 6491PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ. Dephosphorylated at Tyr-649 by PTPN13. Phosphorylation of Tyr-649 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination.By similarity
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ63788.
PRIDEiQ63788.

PTM databases

PhosphoSiteiQ63788.

Expressioni

Gene expression databases

GenevestigatoriQ63788.

Interactioni

Subunit structurei

Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated). Interacts with NYAP1, NYAP2 and MYO16. Interacts with FBXL2; PIK3R2 is a substrate of the SCF(FBXL2) complex. Interacts with PTPN13; dephosphorylates PIK3R2.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ63788.
SMRiQ63788. Positions 4-86, 318-590, 608-712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 8077SH3PROSITE-ProRule annotationAdd
BLAST
Domaini112 – 289178Rho-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini324 – 41996SH2 1PROSITE-ProRule annotationAdd
BLAST
Domaini616 – 71095SH2 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The SH2 2 domain is required for interaction with FBXL2 and PTPN13.By similarity

Sequence similaritiesi

Belongs to the PI3K p85 subunit family.Curated
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
Contains 2 SH2 domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiNOG263689.
HOGENOMiHOG000008438.
HOVERGENiHBG082100.
InParanoidiQ63788.
PhylomeDBiQ63788.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
3.30.505.10. 2 hits.
InterProiIPR001720. PI3kinase_P85.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10155. PTHR10155. 1 hit.
PfamiPF00620. RhoGAP. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]
PRINTSiPR00678. PI3KINASEP85.
PR00401. SH2DOMAIN.
SMARTiSM00324. RhoGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEiPS50238. RHOGAP. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63788-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGAEGFQYR AVYPFRRERP EDLELLPGDL LVVSRVALQA LGVADGGERC
60 70 80 90 100
PHNVGWMPGF NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP
110 120 130 140 150
LDGPSESGHT LASLAEQFSP PESAPPILVK LIEAIEQAEL DSEFYSRPEL
160 170 180 190 200
PAPRTDWSLS DLEQWDRTTL YDAVKGFLLA LPAAVVTPEA ASEAYRAMRE
210 220 230 240 250
VTGPVGLVLE PPTLPLHQAL TLRFLLQHLG RVARRAPSPA TAVHALASAF
260 270 280 290 300
GPLLLRAPPP GGEGDGSEPA PDFPVLLLER LVQEHVDEQD TAPPALPPKP
310 320 330 340 350
SKVKPAPTAL ANGGSTPSLQ DAEWYWGDIS REEVNERLRD TPDGTFLVRD
360 370 380 390 400
ASSKIQGEYT LTLRKGGNNK LIKVFHRDGH YGFSEPLTFC SVVELISHYR
410 420 430 440 450
HESLAQYNAK LDTRLLYPVS KYQQDQVVKE DSVEAVGAQL KVYHQQYQDK
460 470 480 490 500
SREYDQLYEE YTRTSQELQM KRTAIEAFNE TILIFEEQGQ TQEKCSKEYL
510 520 530 540 550
ERFRREGNEK EMQRILLNSE RLKSRIAEIH ESRTKLEQDL RAQASDNREI
560 570 580 590 600
DKRMNSLKPD LMQLRKIRDQ YLVWLTQKGA RQRKINEWLG IKNETEDQYS
610 620 630 640 650
LMEDEDALPH HEERTWYVGK INRTQAEEML SAKRDGTFLI RESSQRGCYA
660 670 680 690 700
CSVVVDGDTK HCVIYRTATG FGFAEPYNLY GSLKELVLHY QHASLVQHND
710 720
ALTVTLAHPV RAPGPGPPPA AR
Length:722
Mass (Da):81,329
Last modified:November 1, 1996 - v1
Checksum:i1208368B9F6F0C95
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64046 mRNA. Translation: BAA10926.1.
UniGeneiRn.22497.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64046 mRNA. Translation: BAA10926.1 .
UniGenei Rn.22497.

3D structure databases

ProteinModelPortali Q63788.
SMRi Q63788. Positions 4-86, 318-590, 608-712.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q63788.

Proteomic databases

PaxDbi Q63788.
PRIDEi Q63788.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

RGDi 68341. Pik3r2.

Phylogenomic databases

eggNOGi NOG263689.
HOGENOMi HOG000008438.
HOVERGENi HBG082100.
InParanoidi Q63788.
PhylomeDBi Q63788.

Enzyme and pathway databases

Reactomei REACT_258050. Nephrin interactions.

Miscellaneous databases

PROi Q63788.

Gene expression databases

Genevestigatori Q63788.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
3.30.505.10. 2 hits.
InterProi IPR001720. PI3kinase_P85.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view ]
PANTHERi PTHR10155. PTHR10155. 1 hit.
Pfami PF00620. RhoGAP. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view ]
PRINTSi PR00678. PI3KINASEP85.
PR00401. SH2DOMAIN.
SMARTi SM00324. RhoGAP. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 2 hits.
PROSITEi PS50238. RHOGAP. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A novel 55-kDa regulatory subunit for phosphatidylinositol 3-kinase structurally similar to p55PIK is generated by alternative splicing of the p85alpha gene."
    Inukai K., Anai M., van Breda E., Hosaka T., Katagiri H., Funaki M., Fukushima Y., Ogihara T., Yazaki Y., Kikuchi M., Oka Y., Asano T.
    J. Biol. Chem. 271:5317-5320(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.

Entry informationi

Entry nameiP85B_RAT
AccessioniPrimary (citable) accession number: Q63788
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3