Q63788 (P85B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase regulatory subunit beta
Short name=PI3-kinase regulatory subunit beta
Short name=PI3K regulatory subunit beta
Short name=PtdIns-3-kinase regulatory subunit beta

Alternative name(s):
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
Short name=PI3-kinase subunit p85-beta
Short name=PtdIns-3-kinase regulatory subunit p85-beta

Gene names

Name:

Pik3r2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	722 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Subunit structure	Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated) By similarity.
Post-translational modification	Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ By similarity.
Sequence similarities	Belongs to the PI3K p85 subunit family. Contains 1 Rho-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	Repeat SH2 domain SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of anti-apoptosis Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	phosphatidylinositol 3-kinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	phosphatidylinositol 3-kinase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 722	722	Phosphatidylinositol 3-kinase regulatory subunit beta		PRO_0000080765
Regions
	Domain	4 – 80	77	SH3
	Domain	112 – 289	178	Rho-GAP
	Domain	324 – 419	96	SH2 1
	Domain	616 – 710	95	SH2 2
Amino acid modifications
	Modified residue	359	1	Phosphotyrosine By similarity
	Modified residue	458	1	Phosphotyrosine By similarity
	Modified residue	461	1	Phosphotyrosine By similarity
	Modified residue	599	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63788 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1208368B9F6F0C95	FASTA	722	81,329
	10 20 30 40 50 60 MAGAEGFQYR AVYPFRRERP EDLELLPGDL LVVSRVALQA LGVADGGERC PHNVGWMPGF 70 80 90 100 110 120 NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP LDGPSESGHT LASLAEQFSP 130 140 150 160 170 180 PESAPPILVK LIEAIEQAEL DSEFYSRPEL PAPRTDWSLS DLEQWDRTTL YDAVKGFLLA 190 200 210 220 230 240 LPAAVVTPEA ASEAYRAMRE VTGPVGLVLE PPTLPLHQAL TLRFLLQHLG RVARRAPSPA 250 260 270 280 290 300 TAVHALASAF GPLLLRAPPP GGEGDGSEPA PDFPVLLLER LVQEHVDEQD TAPPALPPKP 310 320 330 340 350 360 SKVKPAPTAL ANGGSTPSLQ DAEWYWGDIS REEVNERLRD TPDGTFLVRD ASSKIQGEYT 370 380 390 400 410 420 LTLRKGGNNK LIKVFHRDGH YGFSEPLTFC SVVELISHYR HESLAQYNAK LDTRLLYPVS 430 440 450 460 470 480 KYQQDQVVKE DSVEAVGAQL KVYHQQYQDK SREYDQLYEE YTRTSQELQM KRTAIEAFNE 490 500 510 520 530 540 TILIFEEQGQ TQEKCSKEYL ERFRREGNEK EMQRILLNSE RLKSRIAEIH ESRTKLEQDL 550 560 570 580 590 600 RAQASDNREI DKRMNSLKPD LMQLRKIRDQ YLVWLTQKGA RQRKINEWLG IKNETEDQYS 610 620 630 640 650 660 LMEDEDALPH HEERTWYVGK INRTQAEEML SAKRDGTFLI RESSQRGCYA CSVVVDGDTK 670 680 690 700 710 720 HCVIYRTATG FGFAEPYNLY GSLKELVLHY QHASLVQHND ALTVTLAHPV RAPGPGPPPA AR

References

[1]

"A novel 55-kDa regulatory subunit for phosphatidylinositol 3-kinase structurally similar to p55PIK is generated by alternative splicing of the p85alpha gene." Inukai K., Anai M., van Breda E., Hosaka T., Katagiri H., Funaki M., Fukushima Y., Ogihara T., Yazaki Y., Kikuchi M., Oka Y., Asano T. J. Biol. Chem. 271:5317-5320(1996) [PubMed: 8621382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D64046 mRNA. Translation: BAA10926.1.
IPI	IPI00211947.
UniGene	Rn.22497.
3D structure databases
ProteinModelPortal	Q63788.
SMR	Q63788. Positions 4-86, 318-590, 608-712.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63788.
PTM databases
PhosphoSite	Q63788.
Proteomic databases
PRIDE	Q63788.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	NM_022185. rat.
Organism-specific databases
RGD	68341. Pik3r2.
Phylogenomic databases
GeneTree	ENSGT00390000010431.
HOVERGEN	HBG082100.
InParanoid	Q63788.
OrthoDB	EOG49GKG4.
Enzyme and pathway databases
Reactome	REACT_114732. Cell-Cell communication.
Gene expression databases
ArrayExpress	Q63788.
Genevestigator	Q63788.
GermOnline	ENSRNOG00000019228. Rattus norvegicus.
Family and domain databases
InterPro	IPR001720. PI3kinase_P85. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. IPR000980. SH2. IPR001452. SH3_domain. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit. G3DSA:3.30.505.10. SH2. 2 hits.
PANTHER	PTHR10155. PI3kinase_P85. 1 hit.
Pfam	PF00620. RhoGAP. 1 hit. PF00017. SH2. 2 hits. [Graphical view]
PRINTS	PR00678. PI3KINASEP85. PR00401. SH2DOMAIN.
SMART	SM00324. RhoGAP. 1 hit. SM00252. SH2. 2 hits. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS50238. RHOGAP. 1 hit. PS50001. SH2. 2 hits. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P85B_RAT

Accession

Primary (citable) accession number: Q63788

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program