Reviewed,
UniProtKB/Swiss-Prot Q63746 (IKBA_RAT)
Last modified
November 3, 2009.
Version 76.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: NF-kappa-B inhibitor alpha Alternative name(s): I-kappa-B-alpha Short name=IkappaBalpha Short name=IkB-alpha RL/IF-1 | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 314 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription By similarity. |
| Subunit structure | Interacts with RELA; the interaction requires the nuclear import signal. Interacts with NKIRAS1 and NKIRAS2. Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with HBV protein X. Interacts with RWDD3; the interaction enhances sumoylation By similarity. |
| Subcellular location | Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export By similarity. |
| Induction | During liver regeneration. Ref.4 |
| Post-translational modification | Ubiquitinated; subsequent to stimulus-dependent phosphorylation on serines By similarity. Sumoylated; sumoylation requires the presence of the nuclear localization signal By similarity. |
| Sequence similarities | Belongs to the NF-kappa-B inhibitor family. Contains 4 ANK repeats. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Nucleus |
| Domain | ANK repeat Repeat |
| PTM | Isopeptide bond Phosphoprotein Ubl conjugation |
| Gene Ontology (GO) | |
| Biological process | cytoplasmic sequestering of NF-kappaB Inferred from mutant phenotype. Source: RGD |
| Cellular component | I-kappaB/NF-kappaB complex Inferred by curator. Source: RGD cytosolInferred from direct assay. Source: RGD nucleusInferred from direct assay. Source: RGD |
| Molecular function | NF-kappaB binding Inferred from physical interaction. Source: RGD heat shock protein bindingInferred from physical interaction. Source: RGD protein complex bindingInferred from physical interaction. Source: RGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 314 | 314 | NF-kappa-B inhibitor alpha | PRO_0000067002 | |||||
Regions | |||||||||
| Repeat | 110 – 139 | 30 | ANK 1 | ||||||
| Repeat | 143 – 172 | 30 | ANK 2 | ||||||
| Repeat | 182 – 211 | 30 | ANK 3 | ||||||
| Repeat | 216 – 245 | 30 | ANK 4 | ||||||
| Motif | 45 – 54 | 10 | Nuclear export signal By similarity | ||||||
| Motif | 110 – 120 | 11 | Nuclear import signal By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 32 | 1 | Phosphoserine; by IKKA and IKKB By similarity | ||||||
| Modified residue | 36 | 1 | Phosphoserine; by IKKA, IKKB and IKKE By similarity | ||||||
| Modified residue | 42 | 1 | Phosphotyrosine; by Tyr-kinases By similarity | ||||||
| Modified residue | 283 | 1 | Phosphoserine; by CK2 By similarity | ||||||
| Modified residue | 288 | 1 | Phosphoserine; by CK2 By similarity | ||||||
| Modified residue | 291 | 1 | Phosphothreonine; by CK2 By similarity | ||||||
| Modified residue | 293 | 1 | Phosphoserine; by CK2 By similarity | ||||||
| Cross-link | 21 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity | |||||||
| Cross-link | 22 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Potential | |||||||
Sequences
| ||||||||||||||||||
References
| [1] | "Sequence of rat RL/IF-1 encoding an IkappaB, and comparison with related proteins containing Notch-like repeats." Tewari M., Mohn K.L., Yue F.E., Taub R.A. Nucleic Acids Res. 20:607-607(1992) [PubMed: 1741294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | Erratum Tewari M., Mohn K.L., Yue F.E., Taub R.A. Nucleic Acids Res. 20:2624-2624(1992) |
| [3] | Erratum Tewari M., Mohn K.L., Yue F.E., Taub R.A. Nucleic Acids Res. 20:2931-2931(1992) |
| [4] | "Rapid induction in regenerating liver of RL/IF-1 (an I kappa B that inhibits NF-kappa B, RelB-p50, and c-Rel-p50) and PHF, a novel kappa B site-binding complex." Tewari M., Dobrzanski P., Mohn K.L., Cressman D.E., Hsu J.C., Bravo R., Taub R. Mol. Cell. Biol. 12:2898-2908(1992) [PubMed: 1588976] [Abstract] Cited for: INDUCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X63594 mRNA. Translation: CAA45138.1. | |
| IPI | IPI00211841. |
| PIR | A44437. |
| RefSeq | NP_001099190.2. |
| UniGene | Rn.12550 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IKN based on UniProtKB P25963. |
| SMR | Q63746. Positions 70-281. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q63746. |
PTM databases | |
| PhosphoSite | Q63746. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000009894; ENSRNOP00000009894; ENSRNOG00000007390; Rattus norvegicus. [Genome view] |
| GeneID | 25493. |
| KEGG | rno:25493. |
Organism-specific databases | |
| CTD | 25493. |
| RGD | 3171. Nfkbia. |
Phylogenomic databases | |
| HOVERGEN | Q63746. |
| OMA | LKAGCDP. |
Gene expression databases | |
| ArrayExpress | Q63746. |
| Genevestigator | Q63746. |
| GermOnline | ENSRNOG00000007390. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR002110. ANK. IPR015681. NF_kB_inhbitor. [Graphical view] |
| Gene3D | G3DSA:1.25.40.20. ANK. 1 hit. |
| PANTHER | PTHR18958:SF236. NF_kB_inhbitor. 1 hit. |
| Pfam | PF00023. Ank. 5 hits. [Graphical view] |
| SMART | SM00248. ANK. 5 hits. [Graphical view] |
| PROSITE | PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 3 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 606869. |
Entry information
| Entry name | IKBA_RAT | ||||||||
| Accession | Primary (citable) accession number: Q63746 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


