Q63746 (IKBA_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 104.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: NF-kappa-B inhibitor alpha Alternative name(s): I-kappa-B-alpha Short name=IkB-alpha Short name=IkappaBalpha RL/IF-1 | ||||
| Gene names |
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| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus![]() |
Protein attributes
| Sequence length | 314 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription By similarity. |
| Subunit structure | Interacts with RELA; the interaction requires the nuclear import signal. Interacts with NKIRAS1 and NKIRAS2. Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with HBV protein X. Interacts with RWDD3; the interaction enhances sumoylation. Interacts (when phosphorylated at the 2 serine residues in the destruction motif D-S-G-X(2,3,4)-S) with BTRC. Associates with the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC; the association is mediated via interaction with BTRC. Part of a SCF(BTRC)-like complex lacking CUL1, which is associated with RELA; RELA interacts directly with NFKBIA By similarity. Interacts with PRMT2. Interacts with PRKACA in platelets; this interaction is disrupted by thrombin and collagen By similarity. Interacts with HIF1AN By similarity. |
| Subcellular location | Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export By similarity. |
| Induction | During liver regeneration. Ref.6 |
| Post-translational modification | Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation By similarity. Sumoylated; sumoylation requires the presence of the nuclear import signal By similarity. Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36 By similarity. |
| Sequence similarities | Belongs to the NF-kappa-B inhibitor family. Contains 4 ANK repeats. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 314 | 314 | NF-kappa-B inhibitor alpha | PRO_0000067002 | |||||
Regions | |||||||||
| Repeat | 110 – 139 | 30 | ANK 1 | ||||||
| Repeat | 143 – 172 | 30 | ANK 2 | ||||||
| Repeat | 182 – 211 | 30 | ANK 3 | ||||||
| Repeat | 216 – 245 | 30 | ANK 4 | ||||||
| Motif | 30 – 36 | 7 | Destruction motif By similarity | ||||||
| Motif | 45 – 54 | 10 | Nuclear export signal By similarity | ||||||
| Motif | 110 – 120 | 11 | Nuclear import signal By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 32 | 1 | Phosphoserine; by IKKA and IKKB By similarity | ||||||
| Modified residue | 36 | 1 | Phosphoserine; by IKKA, IKKB, IKKE and TBK1 By similarity | ||||||
| Modified residue | 42 | 1 | Phosphotyrosine; by Tyr-kinases By similarity | ||||||
| Modified residue | 210 | 1 | (3S)-3-hydroxyasparagine; by HIF1AN By similarity | ||||||
| Modified residue | 244 | 1 | (3S)-3-hydroxyasparagine; by HIF1AN By similarity | ||||||
| Modified residue | 283 | 1 | Phosphoserine; by CK2 By similarity | ||||||
| Modified residue | 288 | 1 | Phosphoserine; by CK2 By similarity | ||||||
| Modified residue | 291 | 1 | Phosphothreonine; by CK2 By similarity | ||||||
| Modified residue | 293 | 1 | Phosphoserine; by CK2 By similarity | ||||||
| Modified residue | 296 | 1 | Phosphothreonine By similarity | ||||||
| Cross-link | 21 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity | |||||||
| Cross-link | 22 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Potential | |||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence of rat RL/IF-1 encoding an IkappaB, and comparison with related proteins containing Notch-like repeats." Tewari M., Mohn K.L., Yue F.E., Taub R.A. Nucleic Acids Res. 20:607-607(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | Erratum Tewari M., Mohn K.L., Yue F.E., Taub R.A. Nucleic Acids Res. 20:2624-2624(1992) |
| [3] | Erratum Tewari M., Mohn K.L., Yue F.E., Taub R.A. Nucleic Acids Res. 20:2931-2931(1992) |
| [4] | Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary. |
| [6] | "Rapid induction in regenerating liver of RL/IF-1 (an I kappa B that inhibits NF-kappa B, RelB-p50, and c-Rel-p50) and PHF, a novel kappa B site-binding complex." Tewari M., Dobrzanski P., Mohn K.L., Cressman D.E., Hsu J.C., Bravo R., Taub R. Mol. Cell. Biol. 12:2898-2908(1992) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X63594 mRNA. Translation: CAA45138.1. CH473947 Genomic DNA. Translation: EDM03428.1. BC166886 mRNA. Translation: AAI66886.1. |
| IPI | IPI00211841. |
| PIR | A44437. |
| RefSeq | NP_001099190.2. NM_001105720.2. |
| UniGene | Rn.12550. |
3D structure databases | |
| ProteinModelPortal | Q63746. |
| SMR | Q63746. Positions 70-281. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-59941N. |
| STRING | 10116.ENSRNOP00000009894. |
PTM databases | |
| PhosphoSite | Q63746. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSRNOT00000009894; ENSRNOP00000009894; ENSRNOG00000007390. |
| GeneID | 25493. |
| KEGG | rno:25493. |
| UCSC | RGD:3171. rat. |
Organism-specific databases | |
| CTD | 4792. |
| RGD | 3171. Nfkbia. |
Phylogenomic databases | |
| eggNOG | COG0666. |
| GeneTree | ENSGT00550000074527. |
| HOGENOM | HOG000059576. |
| HOVERGEN | HBG018875. |
| InParanoid | Q63746. |
| KO | K04734. |
| OMA | SIHGYLA. |
| OrthoDB | EOG40CHHR. |
Gene expression databases | |
| ArrayExpress | Q63746. |
| Genevestigator | Q63746. |
| GermOnline | ENSRNOG00000007390. Rattus norvegicus. |
Family and domain databases | |
| Gene3D | 1.25.40.20. 1 hit. |
| InterPro | IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. [Graphical view] |
| Pfam | PF12796. Ank_2. 2 hits. [Graphical view] |
| PRINTS | PR01415. ANKYRIN. |
| SMART | SM00248. ANK. 5 hits. [Graphical view] |
| SUPFAM | SSF48403. ANK. 1 hit. |
| PROSITE | PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 3 hits. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 606869. |
Entry information
| Entry name | IKBA_RAT | ||||||||
| Accession | Primary (citable) accession number: Q63746 Secondary accession number(s): B2RYS5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with
