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Protein

Dihydroorotate dehydrogenase (quinone), mitochondrial

Gene

Dhodh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

Catalytic activityi

(S)-dihydroorotate + a quinone = orotate + a quinol.

Cofactori

FMN1 PublicationNote: Binds 1 FMN per subunit.1 Publication

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route).
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydroorotate dehydrogenase (quinone), mitochondrial (Dhodh)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route), the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99Substrate1
Binding sitei119FMN1 Publication1
Binding sitei180FMN1 Publication1
Binding sitei211FMN1 Publication1
Binding sitei211Substrate1
Active sitei214NucleophileBy similarity1
Binding sitei254FMN1 Publication1
Binding sitei282FMN; via carbonyl oxygen1 Publication1
Binding sitei305FMN; via amide nitrogen1 Publication1
Binding sitei334FMN; via amide nitrogen1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 99FMN1 Publication5
Nucleotide bindingi355 – 356FMN1 Publication2

GO - Molecular functioni

  • dihydroorotate dehydrogenase activity Source: RGD
  • drug binding Source: RGD
  • FMN binding Source: RGD
  • ubiquinone binding Source: RGD

GO - Biological processi

  • 'de novo' pyrimidine nucleobase biosynthetic process Source: RGD
  • 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  • female pregnancy Source: RGD
  • lactation Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • regulation of mitochondrial fission Source: RGD
  • response to caffeine Source: RGD
  • response to drug Source: RGD
  • response to L-arginine Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to starvation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BRENDAi1.3.5.2. 5301.
ReactomeiR-RNO-500753. Pyrimidine biosynthesis.
SABIO-RKQ63707.
UniPathwayiUPA00070; UER00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotate dehydrogenase (quinone), mitochondrial (EC:1.3.5.2)
Short name:
DHOdehase
Alternative name(s):
Dihydroorotate oxidase
Gene namesi
Name:Dhodh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi68352. Dhodh.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10Mitochondrial intermembrane10
Transmembranei11 – 30HelicalAdd BLAST20
Topological domaini31 – 395Mitochondrial matrixAdd BLAST365

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: RGD
  • neuronal cell body Source: RGD
  • nucleoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2383.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000298861 – 395Dihydroorotate dehydrogenase (quinone), mitochondrialAdd BLAST395
Transit peptidei1 – 10Mitochondrion; not cleaved10

Post-translational modificationi

The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.

Proteomic databases

PaxDbiQ63707.
PRIDEiQ63707.

PTM databases

iPTMnetiQ63707.
PhosphoSitePlusiQ63707.

Expressioni

Gene expression databases

BgeeiENSRNOG00000015063.
GenevisibleiQ63707. RN.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061315.

Chemistry databases

BindingDBiQ63707.

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 39Combined sources4
Helixi41 – 48Combined sources8
Helixi51 – 63Combined sources13
Helixi76 – 78Combined sources3
Beta strandi80 – 82Combined sources3
Beta strandi85 – 93Combined sources9
Beta strandi101 – 103Combined sources3
Helixi104 – 109Combined sources6
Beta strandi113 – 120Combined sources8
Beta strandi133 – 136Combined sources4
Turni137 – 140Combined sources4
Beta strandi141 – 144Combined sources4
Helixi153 – 162Combined sources10
Helixi164 – 172Combined sources9
Beta strandi177 – 181Combined sources5
Helixi190 – 201Combined sources12
Helixi202 – 204Combined sources3
Beta strandi206 – 211Combined sources6
Helixi226 – 241Combined sources16
Beta strandi243 – 246Combined sources4
Beta strandi250 – 255Combined sources6
Helixi261 – 274Combined sources14
Beta strandi278 – 281Combined sources4
Helixi295 – 298Combined sources4
Beta strandi299 – 305Combined sources7
Helixi306 – 308Combined sources3
Helixi309 – 322Combined sources14
Turni323 – 325Combined sources3
Beta strandi329 – 334Combined sources6
Helixi338 – 346Combined sources9
Beta strandi350 – 355Combined sources6
Helixi356 – 361Combined sources6
Helixi364 – 379Combined sources16
Helixi385 – 388Combined sources4
Helixi391 – 394Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UUMX-ray2.30A/B30-395[»]
1UUOX-ray2.44A30-395[»]
4ORIX-ray1.50A32-395[»]
ProteinModelPortaliQ63707.
SMRiQ63707.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63707.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni144 – 148Substrate binding5
Regioni211 – 216Substrate bindingBy similarity6
Regioni283 – 284Substrate binding2

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1436. Eukaryota.
COG0167. LUCA.
GeneTreeiENSGT00500000044924.
HOGENOMiHOG000225103.
HOVERGENiHBG006898.
InParanoidiQ63707.
KOiK00254.
OMAiERIKMGA.
OrthoDBiEOG091G07JK.
PhylomeDBiQ63707.
TreeFamiTF105973.

Family and domain databases

CDDicd04738. DHOD_2_like. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00225. DHO_dh_type2. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01036. pyrD_sub2. 1 hit.
PROSITEiPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63707-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWRQLRKRA LDAVIILGGG GLLFTSYLTA TGDDHFYAEY LMPGLQRLLD
60 70 80 90 100
PESAHRLAVR VTSLGLLPRA TFQDSDMLEV KVLGHKFRNP VGIAAGFDKN
110 120 130 140 150
GEAVDGLYKL GFGFVEVGSV TPQPQEGNPR PRVFRLPEDQ AVINRYGFNS
160 170 180 190 200
HGLSVVEHRL RARQQKQAQL TADGLPLGIN LGKNKTSEDA AADYAEGVRT
210 220 230 240 250
LGPLADYLVV NVSSPNTAGL RSLQGKTELR HLLSKVLQER DALKGTRKPA
260 270 280 290 300
VLVKIAPDLT AQDKEDIASV ARELGIDGLI VTNTTVSRPV GLQGALRSET
310 320 330 340 350
GGLSGKPLRD LSTQTIREMY ALTQGRIPII GVGGVSSGQD ALEKIQAGAS
360 370 380 390
LVQLYTALIF LGPPVVVRVK RELEALLKER GFTTVTDAIG ADHRR
Length:395
Mass (Da):42,663
Last modified:November 1, 1996 - v1
Checksum:i4C7E1A9C5E5E60EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80778 mRNA. Translation: CAA56765.1.
PIRiA59277. S47435.
RefSeqiNP_001008553.1. NM_001008553.1.
XP_006255663.1. XM_006255601.3.
XP_008770751.1. XM_008772529.2.
UniGeneiRn.81502.

Genome annotation databases

EnsembliENSRNOT00000066733; ENSRNOP00000061315; ENSRNOG00000015063.
GeneIDi65156.
KEGGirno:65156.
UCSCiRGD:68352. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80778 mRNA. Translation: CAA56765.1.
PIRiA59277. S47435.
RefSeqiNP_001008553.1. NM_001008553.1.
XP_006255663.1. XM_006255601.3.
XP_008770751.1. XM_008772529.2.
UniGeneiRn.81502.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UUMX-ray2.30A/B30-395[»]
1UUOX-ray2.44A30-395[»]
4ORIX-ray1.50A32-395[»]
ProteinModelPortaliQ63707.
SMRiQ63707.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061315.

Chemistry databases

BindingDBiQ63707.
ChEMBLiCHEMBL2383.

PTM databases

iPTMnetiQ63707.
PhosphoSitePlusiQ63707.

Proteomic databases

PaxDbiQ63707.
PRIDEiQ63707.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000066733; ENSRNOP00000061315; ENSRNOG00000015063.
GeneIDi65156.
KEGGirno:65156.
UCSCiRGD:68352. rat.

Organism-specific databases

CTDi1723.
RGDi68352. Dhodh.

Phylogenomic databases

eggNOGiKOG1436. Eukaryota.
COG0167. LUCA.
GeneTreeiENSGT00500000044924.
HOGENOMiHOG000225103.
HOVERGENiHBG006898.
InParanoidiQ63707.
KOiK00254.
OMAiERIKMGA.
OrthoDBiEOG091G07JK.
PhylomeDBiQ63707.
TreeFamiTF105973.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00946.
BRENDAi1.3.5.2. 5301.
ReactomeiR-RNO-500753. Pyrimidine biosynthesis.
SABIO-RKQ63707.

Miscellaneous databases

EvolutionaryTraceiQ63707.
PROiQ63707.

Gene expression databases

BgeeiENSRNOG00000015063.
GenevisibleiQ63707. RN.

Family and domain databases

CDDicd04738. DHOD_2_like. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00225. DHO_dh_type2. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01036. pyrD_sub2. 1 hit.
PROSITEiPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRD_RAT
AccessioniPrimary (citable) accession number: Q63707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.