Q636K7 (SYP1_BACCZ) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 55.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Proline--tRNA ligase 1 EC=6.1.1.15 Alternative name(s): Prolyl-tRNA synthetase 1 Short name=ProRS 1 | ||||
| Gene names |
| ||||
| Organism | Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 288681 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group |
Protein attributes
| Sequence length | 566 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569 |
| Catalytic activity | ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569 |
| Subunit structure | Homodimer By similarity. HAMAP MF_01569 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_01569. |
| Domain | Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569 |
| Sequence similarities | Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Aminoacyl-tRNA synthetase Ligase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 566 | 566 | Proline--tRNA ligase 1 HAMAP MF_01569 | PRO_0000248645 | |||
Sequences
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References
| [1] | "Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. Gilna P.J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ZK / E33L. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CP000001 Genomic DNA. Translation: AAU16688.1. |
| RefSeq | YP_085160.1. NC_006274.1. |
3D structure databases | |
| ProteinModelPortal | Q636K7. |
| SMR | Q636K7. Positions 1-565. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q636K7. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBACT00000044602; EBBACP00000043503; EBBACG00000044593. |
| GeneID | 3023526. |
| GenomeReviews | Gene locus BCE33L3578 in contig CP000001_GR. |
| KEGG | bcz:BCZK3578. |
| PATRIC | 18890908. VBIBacCer95304_3792. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG0442. |
| GeneTree | EBGT00050000001167. |
| HOGENOM | HBG403504. |
| OMA | IQPAELW. |
| ProtClustDB | PRK09194. |
Enzyme and pathway databases | |
| BioCyc | BCER288681:BCE33L3578-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01569. Pro_tRNA_synth_type1. [Tree] |
| InterPro | IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit. |
| KO | K01881. |
| PANTHER | PTHR11451:SF3. PTHR11451:SF3. 1 hit. |
| Pfam | PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view] |
| PRINTS | PR01046. TRNASYNTHPRO. |
| SUPFAM | SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit. |
| TIGRFAMs | TIGR00409. ProS_fam_II. 1 hit. |
| PROSITE | PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | SYP1_BACCZ | ||||||||
| Accession | Primary (citable) accession number: Q636K7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Aminoacyl-tRNA synthetases List of aminoacyl-tRNA synthetase entries |
| SIMILARITY comments Index of protein domains and families |

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