Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1-deoxy-D-xylulose 5-phosphate reductoisomerase 2

Gene

dxr2

Organism
Bacillus cereus (strain ZK / E33L)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 1 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase 1 (dxr1), 1-deoxy-D-xylulose 5-phosphate reductoisomerase 2 (dxr2)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211SubstrateUniRule annotation
Metal bindingi146 – 1461Divalent metal cationUniRule annotation
Metal bindingi148 – 1481Divalent metal cationUniRule annotation
Binding sitei148 – 1481SubstrateUniRule annotation
Binding sitei172 – 1721SubstrateUniRule annotation
Binding sitei195 – 1951SubstrateUniRule annotation
Metal bindingi217 – 2171Divalent metal cationUniRule annotation
Binding sitei217 – 2171SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 3630NADPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Metal-binding, NADP

Enzyme and pathway databases

BioCyciBCER288681:GHG7-3643-MONOMER.
UniPathwayiUPA00056; UER00092.

Names & Taxonomyi

Protein namesi
Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase 2UniRule annotation (EC:1.1.1.267UniRule annotation)
Short name:
DXP reductoisomerase 2UniRule annotation
Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase 2UniRule annotation
2-C-methyl-D-erythritol 4-phosphate synthase 2UniRule annotation
Gene namesi
Name:dxr2UniRule annotation
Ordered Locus Names:BCE33L3580
OrganismiBacillus cereus (strain ZK / E33L)
Taxonomic identifieri288681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000002612 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3803801-deoxy-D-xylulose 5-phosphate reductoisomerase 2PRO_0000163606Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ636K5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DXR family.UniRule annotation

Phylogenomic databases

KOiK00099.
OMAiGFCPLSE.
OrthoDBiEOG6R2H04.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00183. DXP_reductoisom.
InterProiIPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006205. Dxp_reductismrs. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69055. SSF69055. 1 hit.
TIGRFAMsiTIGR00243. Dxr. 1 hit.

Sequencei

Sequence statusi: Complete.

Q636K5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNISLLGAS GSIGTQTLDV LRSHPDQFRL VAFSVGKNID YAVKVIQEFS
60 70 80 90 100
PQIVSVQREE DVLKLQAVSG NTKIVYGSEG LLEVALHPDA EIVVNAVVGS
110 120 130 140 150
VGLLPTLRAI EAKKTIGIAN KETLVTAGHL VMEAARKHNV SLLPVDSEHS
160 170 180 190 200
AIFQCLNGEN EKRISRLIIT ASGGSFRDKT RDELHHVTVE DALRHPNWSM
210 220 230 240 250
GSKITIDSAT MMNKGLEVIE AHWLFGIPYE QIDVVLHKES IIHSMVEFED
260 270 280 290 300
RSVMAQLGSP DMRVPIQYAL TYPDRLPLSD TKQLNLWEIG TLHFEKMNQE
310 320 330 340 350
RFRCLRFAYE AGKAGGSMPA VMNAANEVAV EAFLQKRIGF LTVEDLIEKA
360 370 380
MNHHNVIARP SLEEILEIDA ATRRFVMEQI
Length:380
Mass (Da):42,239
Last modified:October 25, 2004 - v1
Checksum:i689DDF159E83D1B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000001 Genomic DNA. Translation: AAU16686.1.
RefSeqiWP_000790359.1. NZ_CP009968.1.

Genome annotation databases

EnsemblBacteriaiAAU16686; AAU16686; BCE33L3580.
KEGGibcz:BCE33L3580.
PATRICi18890912. VBIBacCer95304_3794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000001 Genomic DNA. Translation: AAU16686.1.
RefSeqiWP_000790359.1. NZ_CP009968.1.

3D structure databases

ProteinModelPortaliQ636K5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU16686; AAU16686; BCE33L3580.
KEGGibcz:BCE33L3580.
PATRICi18890912. VBIBacCer95304_3794.

Phylogenomic databases

KOiK00099.
OMAiGFCPLSE.
OrthoDBiEOG6R2H04.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00092.
BioCyciBCER288681:GHG7-3643-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00183. DXP_reductoisom.
InterProiIPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006205. Dxp_reductismrs. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69055. SSF69055. 1 hit.
TIGRFAMsiTIGR00243. Dxr. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ZK / E33L.

Entry informationi

Entry nameiDXR2_BACCZ
AccessioniPrimary (citable) accession number: Q636K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: October 25, 2004
Last modified: April 13, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.