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Q636G2

- RLMN_BACCZ

UniProt

Q636G2 - RLMN_BACCZ

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Protein
Probable dual-specificity RNA methyltransferase RlmN
Gene
rlmN, BCE33L3623
Organism
Bacillus cereus (strain ZK / E33L)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs By similarity.UniRule annotation

Catalytic activityi

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA.UniRule annotation
2 S-adenosyl-L-methionine + adenine(37) in tRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei105 – 1051Proton acceptor Reviewed prediction
Metal bindingi125 – 1251Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi129 – 1291Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi132 – 1321Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Binding sitei207 – 2071S-adenosyl-L-methionine By similarity
Binding sitei306 – 3061S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity
Active sitei349 – 3491S-methylcysteine intermediate By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. rRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
  4. rRNA binding Source: UniProtKB-HAMAP
  5. tRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
  6. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. rRNA base methylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBCER288681:GHG7-3686-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dual-specificity RNA methyltransferase RlmN (EC:2.1.1.-, EC:2.1.1.192)
Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferase
23S rRNA m2A2503 methyltransferase
Ribosomal RNA large subunit methyltransferase N
tRNA (adenine(37)-C(2))-methyltransferase
tRNA m2A37 methyltransferase
Gene namesi
Name:rlmN
Ordered Locus Names:BCE33L3623
OrganismiBacillus cereus (strain ZK / E33L)
Taxonomic identifieri288681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000002612: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Probable dual-specificity RNA methyltransferase RlmNUniRule annotation
PRO_0000350030Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi118 ↔ 349(transient) By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi288681.BCZK3623.

Structurei

3D structure databases

ProteinModelPortaliQ636G2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 1762S-adenosyl-L-methionine binding By similarity
Regioni230 – 2323S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0820.
KOiK06941.
OMAiPEAPYAK.
OrthoDBiEOG6DJZ2N.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01849. RNA_methyltr_RlmN.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR30544. PTHR30544. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF006004. CHP00048. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00048. TIGR00048. 1 hit.

Sequencei

Sequence statusi: Complete.

Q636G2-1 [UniParc]FASTAAdd to Basket

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METTVRKQKK NLETKKPSIY SLQLHEMQDW LKEQGEPKFR AGQIFDWLYK    50
KRVKNYEDMS NLSKGLREKL SNSFDITTLN TLVKQTSSDG TIKFLFQLYD 100
GYSIETVLMR HEYGNSICVT TQVGCRIGCT FCASTLGGLK RNLEAGEIVA 150
QVVEVQRALD ESEERVSSLV VMGIGEPFDN YDNLMGFLRI INHEKGLHIG 200
ARHMTVSTSG IIPKIYKFAE EDLQINFAIS LHAPNSELRS KLMPINRAYK 250
LPDLMEAIKY YVNRTGRRIT FEYGLFGGEN DQVEHAEELA ALLKGVKCHV 300
NLIPVNYVPE RDYVRTPREQ IFLFEKTLKD RGVNVTIRRE QGHDIDAACG 350
QLRAKERKEE TR 362
Length:362
Mass (Da):41,553
Last modified:October 25, 2004 - v1
Checksum:i5D1D0D3E937245BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000001 Genomic DNA. Translation: AAU16643.1.
RefSeqiYP_085205.1. NC_006274.1.

Genome annotation databases

EnsemblBacteriaiAAU16643; AAU16643; BCE33L3623.
GeneIDi3026775.
KEGGibcz:BCZK3623.
PATRICi18890998. VBIBacCer95304_3837.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000001 Genomic DNA. Translation: AAU16643.1 .
RefSeqi YP_085205.1. NC_006274.1.

3D structure databases

ProteinModelPortali Q636G2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 288681.BCZK3623.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU16643 ; AAU16643 ; BCE33L3623 .
GeneIDi 3026775.
KEGGi bcz:BCZK3623.
PATRICi 18890998. VBIBacCer95304_3837.

Phylogenomic databases

eggNOGi COG0820.
KOi K06941.
OMAi PEAPYAK.
OrthoDBi EOG6DJZ2N.

Enzyme and pathway databases

BioCyci BCER288681:GHG7-3686-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01849. RNA_methyltr_RlmN.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR30544. PTHR30544. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF006004. CHP00048. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00048. TIGR00048. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ZK / E33L.

Entry informationi

Entry nameiRLMN_BACCZ
AccessioniPrimary (citable) accession number: Q636G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 25, 2004
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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