Reviewed, UniProtKB/Swiss-Prot Q636B3 (MRAY_BACCZ)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	BCE33L3672

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_0000108777
Regions
	Transmembrane	5 – 25	21	Potential
	Transmembrane	52 – 72	21	Potential
	Transmembrane	77 – 97	21	Potential
	Transmembrane	122 – 142	21	Potential
	Transmembrane	149 – 169	21	Potential
	Transmembrane	176 – 196	21	Potential
	Transmembrane	201 – 221	21	Potential
	Transmembrane	227 – 247	21	Potential
	Transmembrane	253 – 273	21	Potential
	Transmembrane	302 – 322	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q636B3-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: FC5E78105473854E	FASTA	324	34,906
	10 20 30 40 50 60 MLEQGLLVTA GVAFLISVAL SPLFIPFLRK LKFGQSIRDE GPKSHQKKSG TPTMGGIVIY 70 80 90 100 110 120 VSMMVTSLIM AIKFNHLGAE VSLLLLVTFG YGLIGFLDDY IKVVKKRNLG LTSKQKLVGQ 130 140 150 160 170 180 LVIAIAFFLI GKGQAFHTYI MIPGTDVKFE LGWAYFVLVL FMLIGGSNAV NLTDGLDGLL 190 200 210 220 230 240 SGTAAIAFGA FSIIAVAQEQ FGVAIFCMAV VGAVLGFLVF NANPAKVFMG DTGSLALGGA 250 260 270 280 290 300 IAAVAILLKQ ELLLVIIGGV FVMETLSVII QVISFKTTGK RVFKMSPLHH HYELCGWSEW 310 320 RVVVTFWSVG FLLAVLGIYI GVWM

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000001 Genomic DNA. Translation: AAU16594.1.
RefSeq	YP_085254.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q636B3.
Genome annotation databases
GeneID	3025476.
GenomeReviews	Gene locus BCE33L3672 in contig CP000001_GR.
KEGG	bcz:BCZK3672.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q636B3.
OMA	HHHFEQK.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L3672-MON.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. False negative. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BACCZ

Accession

Primary (citable) accession number: Q636B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)