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Q63691 (CD14_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen=CD14
Gene names
Name:Cd14
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules By similarity.

Subunit structure

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4 By similarity.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Sequence similarities

Contains 11 LRR (leucine-rich) repeats.

Ontologies

Keywords
   Biological processImmunity
Inflammatory response
Innate immunity
   Cellular componentCell membrane
Membrane
   DomainLeucine-rich repeat
Repeat
Signal
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to lipopolysaccharide

Inferred from expression pattern PubMed 22511970. Source: RGD

cellular response to lipoteichoic acid

Inferred from electronic annotation. Source: Ensembl

inflammatory response

Inferred from direct assay PubMed 12218159. Source: RGD

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of cytokine secretion

Inferred from mutant phenotype PubMed 12046090. Source: RGD

positive regulation of endocytosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of tumor necrosis factor production

Inferred from electronic annotation. Source: Ensembl

response to electrical stimulus

Inferred from expression pattern PubMed 19464360. Source: RGD

response to ethanol

Inferred from expression pattern PubMed 10414605. Source: RGD

response to heat

Inferred from expression pattern PubMed 12435950. Source: RGD

response to lipopolysaccharide

Inferred from expression pattern PubMed 16631199. Source: RGD

response to magnesium ion

Inferred from expression pattern PubMed 16180088. Source: RGD

response to tumor necrosis factor

Inferred from mutant phenotype PubMed 10777809. Source: RGD

   Cellular_componentanchored component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

cell surface

Inferred from direct assay PubMed 12046090. Source: RGD

extracellular space

Inferred from direct assay PubMed 12835948. Source: RGD

lipopolysaccharide receptor complex

Traceable author statement PubMed 12218159. Source: RGD

membrane raft

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay PubMed 18787027. Source: RGD

   Molecular_functionlipopolysaccharide binding

Inferred from electronic annotation. Source: Ensembl

lipoteichoic acid binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 18787027. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 342325Monocyte differentiation antigen CD14
PRO_0000020891
Propeptide343 – 37230Removed in mature form Potential
PRO_0000020892

Regions

Repeat57 – 8428LRR 1
Repeat85 – 12036LRR 2
Repeat121 – 14626LRR 3
Repeat147 – 17428LRR 4
Repeat175 – 19824LRR 5
Repeat199 – 22628LRR 6
Repeat227 – 25327LRR 7
Repeat254 – 27825LRR 8
Repeat279 – 29921LRR 9
Repeat300 – 32122LRR 10
Repeat322 – 34625LRR 11
Region44 – 9148Ligand-binding pocket rim By similarity

Amino acid modifications

Lipidation3421GPI-anchor amidated asparagine Potential
Glycosylation391N-linked (GlcNAc...) Potential
Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation1531N-linked (GlcNAc...) Potential
Glycosylation1861N-linked (GlcNAc...) Potential
Glycosylation2821N-linked (GlcNAc...) Potential
Disulfide bond25 ↔ 38 By similarity
Disulfide bond36 ↔ 53 By similarity
Disulfide bond189 ↔ 219 By similarity
Disulfide bond243 ↔ 272 By similarity

Experimental info

Sequence conflict561Missing in AAB01154. Ref.1
Sequence conflict2201P → S in AAB01154. Ref.1
Sequence conflict2691T → A in AAB01154. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q63691 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: E99DD2C551DBBE69

FASTA37240,054
        10         20         30         40         50         60 
MKLMLGLLLL PLTLVHASPA TPEPCELDQD EESVRCYCNF SDPQPNWSSA FLCAGAEDVE 

        70         80         90        100        110        120 
FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS LPLKRLTVRS ARVPTQILFG TLRVLGYSGL 

       130        140        150        160        170        180 
RELTLENLEV TGTALSPLLD ATGPDLNTLS LRNVSWATTD TWLAELQQWL KPGLKVLSIA 

       190        200        210        220        230        240 
QAHSLNFSCK QVGVFPALAT LDLSDNPELG EKGLISALCP HKFPTLQVLA LRNAGMETTS 

       250        260        270        280        290        300 
GVCSALAAAR VPLQALDLSH NSLRDTAGTP SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS 

       310        320        330        340        350        360 
VLDLSYNRLD RKPRPEELPE VGSLSLTGNP FLHSESQSEA YNSGVVIATA LSPGSAGLSG 

       370 
TLALLLGHRL FV 

« Hide

References

« Hide 'large scale' references
[1]"CD14 mediate endotoxin induction of nitric oxide synthase in cultured brain glial cells."
Galea E., Reis D.J., Fox E.S., Xu H., Feinstein D.L.
J. Neuroimmunol. 64:19-28(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.
[2]"Primary structure of rat CD14 and characteristics of rat CD14, cytokine, and NO synthase mRNA expression in mononuclear phagocyte system cells in response to LPS."
Takai N., Kataoka M., Higuchi Y., Matsuura K., Yamamoto S.
J. Leukoc. Biol. 61:736-744(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Expression of CD14 by hepatocytes: upregulation by cytokines during endotoxemia."
Liu S., Khemlani L.S., Shapiro R.A., Johnson M.L., Liu K., Geller D.A., Watkins S.C., Goyert S.M., Billiar T.R.
Infect. Immun. 66:5089-5098(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51804 mRNA. Translation: AAB01154.1.
AF087943 mRNA. Translation: AAC35371.1.
BC061733 mRNA. Translation: AAH61733.1.
RefSeqNP_068512.1. NM_021744.1.
UniGeneRn.42942.

3D structure databases

ProteinModelPortalQ63691.
SMRQ63691. Positions 20-335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000023977.

Proteomic databases

PaxDbQ63691.
PRIDEQ63691.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023977; ENSRNOP00000023977; ENSRNOG00000017819.
GeneID60350.
KEGGrno:60350.
UCSCRGD:620588. rat.

Organism-specific databases

CTD929.
RGD620588. Cd14.

Phylogenomic databases

eggNOGNOG86091.
GeneTreeENSGT00390000005689.
HOGENOMHOG000237268.
HOVERGENHBG005269.
InParanoidQ63691.
KOK04391.
OMAWATGGAW.
OrthoDBEOG751NFQ.
PhylomeDBQ63691.
TreeFamTF338550.

Gene expression databases

GenevestigatorQ63691.

Family and domain databases

InterProIPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PIRSFPIRSF002017. CD14. 1 hit.
PROSITEPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio612011.
PROQ63691.

Entry information

Entry nameCD14_RAT
AccessionPrimary (citable) accession number: Q63691
Secondary accession number(s): Q6GT04
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 2007
Last modified: June 11, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families