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Protein

Monocyte differentiation antigen CD14

Gene

Cd14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processImmunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-RNO-166016 Toll Like Receptor 4 (TLR4) Cascade
R-RNO-166020 Transfer of LPS from LBP carrier to CD14
R-RNO-5686938 Regulation of TLR by endogenous ligand
R-RNO-6798695 Neutrophil degranulation
R-RNO-936964 Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
R-RNO-937072 TRAF6-mediated induction of TAK1 complex within TLR4 complex

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Gene namesi
Name:Cd14
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi620588 Cd14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000002089118 – 342Monocyte differentiation antigen CD14Add BLAST325
PropeptideiPRO_0000020892343 – 372Removed in mature formSequence analysisAdd BLAST30

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi25 ↔ 38By similarity
Disulfide bondi36 ↔ 53By similarity
Glycosylationi39N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi186N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi189 ↔ 219By similarity
Disulfide bondi243 ↔ 272By similarity
Glycosylationi282N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi342GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ63691
PRIDEiQ63691

PTM databases

SwissPalmiQ63691

Expressioni

Tissue specificityi

Detected in macrophages and peripheral blood monocytes.1 Publication

Inductioni

Up-regulated in Kuppfer cells exposed to bacterial lipopolysaccharide (LPS).1 Publication

Gene expression databases

BgeeiENSRNOG00000017819
GenevisibleiQ63691 RN

Interactioni

Subunit structurei

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Interacts with LPS-bound LPB. Interacts with LPAR1. Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively. Interacts with MYO18A.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023977

Structurei

3D structure databases

ProteinModelPortaliQ63691
SMRiQ63691
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 84LRR 1Add BLAST28
Repeati85 – 120LRR 2Add BLAST36
Repeati121 – 146LRR 3Add BLAST26
Repeati147 – 174LRR 4Add BLAST28
Repeati175 – 198LRR 5Add BLAST24
Repeati199 – 226LRR 6Add BLAST28
Repeati227 – 253LRR 7Add BLAST27
Repeati254 – 278LRR 8Add BLAST25
Repeati279 – 299LRR 9Add BLAST21
Repeati300 – 321LRR 10Add BLAST22
Repeati322 – 346LRR 11Add BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni290 – 372Required for response to bacterial lipopolysaccharide (LPS)By similarityAdd BLAST83

Domaini

The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS.By similarity

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IXG9 Eukaryota
ENOG41118N2 LUCA
GeneTreeiENSGT00390000005689
HOGENOMiHOG000237268
HOVERGENiHBG005269
InParanoidiQ63691
KOiK04391
OMAiLCPHKFP
OrthoDBiEOG091G0DA3
PhylomeDBiQ63691
TreeFamiTF338550

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
IPR016337 Monocyte_diff_Ag_CD14
PANTHERiPTHR10630 PTHR10630, 1 hit
PfamiView protein in Pfam
PF13516 LRR_6, 1 hit
PIRSFiPIRSF002017 CD14, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 4 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLMLGLLLL PLTLVHASPA TPEPCELDQD EESVRCYCNF SDPQPNWSSA
60 70 80 90 100
FLCAGAEDVE FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS LPLKRLTVRS
110 120 130 140 150
ARVPTQILFG TLRVLGYSGL RELTLENLEV TGTALSPLLD ATGPDLNTLS
160 170 180 190 200
LRNVSWATTD TWLAELQQWL KPGLKVLSIA QAHSLNFSCK QVGVFPALAT
210 220 230 240 250
LDLSDNPELG EKGLISALCP HKFPTLQVLA LRNAGMETTS GVCSALAAAR
260 270 280 290 300
VPLQALDLSH NSLRDTAGTP SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS
310 320 330 340 350
VLDLSYNRLD RKPRPEELPE VGSLSLTGNP FLHSESQSEA YNSGVVIATA
360 370
LSPGSAGLSG TLALLLGHRL FV
Length:372
Mass (Da):40,054
Last modified:May 1, 2007 - v2
Checksum:iE99DD2C551DBBE69
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56Missing in AAB01154 (PubMed:8598386).Curated1
Sequence conflicti220P → S in AAB01154 (PubMed:8598386).Curated1
Sequence conflicti269T → A in AAB01154 (PubMed:8598386).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51804 mRNA Translation: AAB01154.1
AF087943 mRNA Translation: AAC35371.1
BC061733 mRNA Translation: AAH61733.1
RefSeqiNP_068512.1, NM_021744.1
UniGeneiRn.42942

Genome annotation databases

EnsembliENSRNOT00000023977; ENSRNOP00000023977; ENSRNOG00000017819
GeneIDi60350
KEGGirno:60350
UCSCiRGD:620588 rat

Similar proteinsi

Entry informationi

Entry nameiCD14_RAT
AccessioniPrimary (citable) accession number: Q63691
Secondary accession number(s): Q6GT04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health