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Q63691

- CD14_RAT

UniProt

Q63691 - CD14_RAT

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Protein

Monocyte differentiation antigen CD14

Gene

Cd14

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity).By similarity

GO - Molecular functioni

  1. lipopolysaccharide binding Source: Ensembl
  2. lipoteichoic acid binding Source: Ensembl

GO - Biological processi

  1. cellular response to lipopolysaccharide Source: RGD
  2. cellular response to lipoteichoic acid Source: Ensembl
  3. inflammatory response Source: RGD
  4. innate immune response Source: UniProtKB-KW
  5. positive regulation of cytokine secretion Source: RGD
  6. positive regulation of endocytosis Source: Ensembl
  7. positive regulation of tumor necrosis factor production Source: Ensembl
  8. response to electrical stimulus Source: RGD
  9. response to ethanol Source: RGD
  10. response to heat Source: RGD
  11. response to lipopolysaccharide Source: RGD
  12. response to magnesium ion Source: RGD
  13. response to tumor necrosis factor Source: RGD
Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_194376. IKK complex recruitment mediated by RIP1.
REACT_198288. TRIF-mediated programmed cell death.
REACT_198983. TRAF6 mediated induction of TAK1 complex.
REACT_247122. Toll Like Receptor 4 (TLR4) Cascade.
REACT_250582. Toll Like Receptor TLR6:TLR2 Cascade.
REACT_252403. MyD88:Mal cascade initiated on plasma membrane.
REACT_255307. Transfer of LPS from LBP carrier to CD14.
REACT_255658. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Gene namesi
Name:Cd14
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 18

Organism-specific databases

RGDi620588. Cd14.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cell surface Source: RGD
  3. extracellular space Source: RGD
  4. extracellular vesicular exosome Source: Ensembl
  5. lipopolysaccharide receptor complex Source: RGD
  6. membrane raft Source: Ensembl
  7. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 342325Monocyte differentiation antigen CD14PRO_0000020891Add
BLAST
Propeptidei343 – 37230Removed in mature formSequence AnalysisPRO_0000020892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 38By similarity
Disulfide bondi36 ↔ 53By similarity
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi189 ↔ 219By similarity
Disulfide bondi243 ↔ 272By similarity
Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
Lipidationi342 – 3421GPI-anchor amidated asparagineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ63691.
PRIDEiQ63691.

Expressioni

Gene expression databases

GenevestigatoriQ63691.

Interactioni

Subunit structurei

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023977.

Structurei

3D structure databases

ProteinModelPortaliQ63691.
SMRiQ63691. Positions 20-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati57 – 8428LRR 1Add
BLAST
Repeati85 – 12036LRR 2Add
BLAST
Repeati121 – 14626LRR 3Add
BLAST
Repeati147 – 17428LRR 4Add
BLAST
Repeati175 – 19824LRR 5Add
BLAST
Repeati199 – 22628LRR 6Add
BLAST
Repeati227 – 25327LRR 7Add
BLAST
Repeati254 – 27825LRR 8Add
BLAST
Repeati279 – 29921LRR 9Add
BLAST
Repeati300 – 32122LRR 10Add
BLAST
Repeati322 – 34625LRR 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni44 – 9148Ligand-binding pocket rimBy similarityAdd
BLAST

Sequence similaritiesi

Contains 11 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiNOG86091.
GeneTreeiENSGT00390000005689.
HOGENOMiHOG000237268.
HOVERGENiHBG005269.
InParanoidiQ63691.
KOiK04391.
OMAiWATGGAW.
OrthoDBiEOG751NFQ.
PhylomeDBiQ63691.
TreeFamiTF338550.

Family and domain databases

InterProiIPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63691-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLMLGLLLL PLTLVHASPA TPEPCELDQD EESVRCYCNF SDPQPNWSSA
60 70 80 90 100
FLCAGAEDVE FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS LPLKRLTVRS
110 120 130 140 150
ARVPTQILFG TLRVLGYSGL RELTLENLEV TGTALSPLLD ATGPDLNTLS
160 170 180 190 200
LRNVSWATTD TWLAELQQWL KPGLKVLSIA QAHSLNFSCK QVGVFPALAT
210 220 230 240 250
LDLSDNPELG EKGLISALCP HKFPTLQVLA LRNAGMETTS GVCSALAAAR
260 270 280 290 300
VPLQALDLSH NSLRDTAGTP SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS
310 320 330 340 350
VLDLSYNRLD RKPRPEELPE VGSLSLTGNP FLHSESQSEA YNSGVVIATA
360 370
LSPGSAGLSG TLALLLGHRL FV
Length:372
Mass (Da):40,054
Last modified:May 1, 2007 - v2
Checksum:iE99DD2C551DBBE69
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561Missing in AAB01154. (PubMed:8598386)Curated
Sequence conflicti220 – 2201P → S in AAB01154. (PubMed:8598386)Curated
Sequence conflicti269 – 2691T → A in AAB01154. (PubMed:8598386)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51804 mRNA. Translation: AAB01154.1.
AF087943 mRNA. Translation: AAC35371.1.
BC061733 mRNA. Translation: AAH61733.1.
RefSeqiNP_068512.1. NM_021744.1.
UniGeneiRn.42942.

Genome annotation databases

EnsembliENSRNOT00000023977; ENSRNOP00000023977; ENSRNOG00000017819.
GeneIDi60350.
KEGGirno:60350.
UCSCiRGD:620588. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51804 mRNA. Translation: AAB01154.1 .
AF087943 mRNA. Translation: AAC35371.1 .
BC061733 mRNA. Translation: AAH61733.1 .
RefSeqi NP_068512.1. NM_021744.1.
UniGenei Rn.42942.

3D structure databases

ProteinModelPortali Q63691.
SMRi Q63691. Positions 20-335.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000023977.

Proteomic databases

PaxDbi Q63691.
PRIDEi Q63691.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000023977 ; ENSRNOP00000023977 ; ENSRNOG00000017819 .
GeneIDi 60350.
KEGGi rno:60350.
UCSCi RGD:620588. rat.

Organism-specific databases

CTDi 929.
RGDi 620588. Cd14.

Phylogenomic databases

eggNOGi NOG86091.
GeneTreei ENSGT00390000005689.
HOGENOMi HOG000237268.
HOVERGENi HBG005269.
InParanoidi Q63691.
KOi K04391.
OMAi WATGGAW.
OrthoDBi EOG751NFQ.
PhylomeDBi Q63691.
TreeFami TF338550.

Enzyme and pathway databases

Reactomei REACT_194376. IKK complex recruitment mediated by RIP1.
REACT_198288. TRIF-mediated programmed cell death.
REACT_198983. TRAF6 mediated induction of TAK1 complex.
REACT_247122. Toll Like Receptor 4 (TLR4) Cascade.
REACT_250582. Toll Like Receptor TLR6:TLR2 Cascade.
REACT_252403. MyD88:Mal cascade initiated on plasma membrane.
REACT_255307. Transfer of LPS from LBP carrier to CD14.
REACT_255658. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.

Miscellaneous databases

NextBioi 612011.
PROi Q63691.

Gene expression databases

Genevestigatori Q63691.

Family and domain databases

InterProi IPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view ]
PIRSFi PIRSF002017. CD14. 1 hit.
PROSITEi PS51450. LRR. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "CD14 mediate endotoxin induction of nitric oxide synthase in cultured brain glial cells."
    Galea E., Reis D.J., Fox E.S., Xu H., Feinstein D.L.
    J. Neuroimmunol. 64:19-28(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "Primary structure of rat CD14 and characteristics of rat CD14, cytokine, and NO synthase mRNA expression in mononuclear phagocyte system cells in response to LPS."
    Takai N., Kataoka M., Higuchi Y., Matsuura K., Yamamoto S.
    J. Leukoc. Biol. 61:736-744(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Expression of CD14 by hepatocytes: upregulation by cytokines during endotoxemia."
    Liu S., Khemlani L.S., Shapiro R.A., Johnson M.L., Liu K., Geller D.A., Watkins S.C., Goyert S.M., Billiar T.R.
    Infect. Immun. 66:5089-5098(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiCD14_RAT
AccessioniPrimary (citable) accession number: Q63691
Secondary accession number(s): Q6GT04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 2007
Last modified: November 26, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3