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Protein

Monocyte differentiation antigen CD14

Gene

Cd14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to lipopolysaccharide Source: RGD
  • cellular response to lipoteichoic acid Source: Ensembl
  • inflammatory response Source: RGD
  • innate immune response Source: UniProtKB-KW
  • positive regulation of cytokine secretion Source: RGD
  • positive regulation of endocytosis Source: Ensembl
  • positive regulation of tumor necrosis factor production Source: Ensembl
  • positive regulation of type I interferon production Source: Ensembl
  • receptor-mediated endocytosis Source: Ensembl
  • response to electrical stimulus Source: RGD
  • response to ethanol Source: RGD
  • response to heat Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to magnesium ion Source: RGD
  • response to tumor necrosis factor Source: RGD
Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_279623. Ligand-dependent caspase activation.
REACT_291198. TRAF6 mediated induction of TAK1 complex.
REACT_292892. MyD88-independent TLR3/TLR4 cascade.
REACT_300799. MyD88:Mal cascade initiated on plasma membrane.
REACT_323385. Toll Like Receptor 4 (TLR4) Cascade.
REACT_325185. TRIF-mediated programmed cell death.
REACT_325676. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
REACT_326555. IKK complex recruitment mediated by RIP1.
REACT_344481. Toll Like Receptor TLR6:TLR2 Cascade.
REACT_350755. Transfer of LPS from LBP carrier to CD14.

Names & Taxonomyi

Protein namesi
Recommended name:
Monocyte differentiation antigen CD14
Alternative name(s):
Myeloid cell-specific leucine-rich glycoprotein
CD_antigen: CD14
Gene namesi
Name:Cd14
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi620588. Cd14.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • cell surface Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: RGD
  • lipopolysaccharide receptor complex Source: RGD
  • membrane raft Source: Ensembl
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 342325Monocyte differentiation antigen CD14PRO_0000020891Add
BLAST
Propeptidei343 – 37230Removed in mature formSequence AnalysisPRO_0000020892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 38By similarity
Disulfide bondi36 ↔ 53By similarity
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi189 ↔ 219By similarity
Disulfide bondi243 ↔ 272By similarity
Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
Lipidationi342 – 3421GPI-anchor amidated asparagineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ63691.
PRIDEiQ63691.

Expressioni

Gene expression databases

GenevisibleiQ63691. RN.

Interactioni

Subunit structurei

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023977.

Structurei

3D structure databases

ProteinModelPortaliQ63691.
SMRiQ63691. Positions 20-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati57 – 8428LRR 1Add
BLAST
Repeati85 – 12036LRR 2Add
BLAST
Repeati121 – 14626LRR 3Add
BLAST
Repeati147 – 17428LRR 4Add
BLAST
Repeati175 – 19824LRR 5Add
BLAST
Repeati199 – 22628LRR 6Add
BLAST
Repeati227 – 25327LRR 7Add
BLAST
Repeati254 – 27825LRR 8Add
BLAST
Repeati279 – 29921LRR 9Add
BLAST
Repeati300 – 32122LRR 10Add
BLAST
Repeati322 – 34625LRR 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni44 – 9148Ligand-binding pocket rimBy similarityAdd
BLAST

Sequence similaritiesi

Contains 11 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiNOG86091.
GeneTreeiENSGT00390000005689.
HOGENOMiHOG000237268.
HOVERGENiHBG005269.
InParanoidiQ63691.
KOiK04391.
OMAiLCPHKFP.
OrthoDBiEOG751NFQ.
PhylomeDBiQ63691.
TreeFamiTF338550.

Family and domain databases

InterProiIPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLMLGLLLL PLTLVHASPA TPEPCELDQD EESVRCYCNF SDPQPNWSSA
60 70 80 90 100
FLCAGAEDVE FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS LPLKRLTVRS
110 120 130 140 150
ARVPTQILFG TLRVLGYSGL RELTLENLEV TGTALSPLLD ATGPDLNTLS
160 170 180 190 200
LRNVSWATTD TWLAELQQWL KPGLKVLSIA QAHSLNFSCK QVGVFPALAT
210 220 230 240 250
LDLSDNPELG EKGLISALCP HKFPTLQVLA LRNAGMETTS GVCSALAAAR
260 270 280 290 300
VPLQALDLSH NSLRDTAGTP SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS
310 320 330 340 350
VLDLSYNRLD RKPRPEELPE VGSLSLTGNP FLHSESQSEA YNSGVVIATA
360 370
LSPGSAGLSG TLALLLGHRL FV
Length:372
Mass (Da):40,054
Last modified:May 1, 2007 - v2
Checksum:iE99DD2C551DBBE69
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561Missing in AAB01154 (PubMed:8598386).Curated
Sequence conflicti220 – 2201P → S in AAB01154 (PubMed:8598386).Curated
Sequence conflicti269 – 2691T → A in AAB01154 (PubMed:8598386).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51804 mRNA. Translation: AAB01154.1.
AF087943 mRNA. Translation: AAC35371.1.
BC061733 mRNA. Translation: AAH61733.1.
RefSeqiNP_068512.1. NM_021744.1.
UniGeneiRn.42942.

Genome annotation databases

EnsembliENSRNOT00000023977; ENSRNOP00000023977; ENSRNOG00000017819.
GeneIDi60350.
KEGGirno:60350.
UCSCiRGD:620588. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51804 mRNA. Translation: AAB01154.1.
AF087943 mRNA. Translation: AAC35371.1.
BC061733 mRNA. Translation: AAH61733.1.
RefSeqiNP_068512.1. NM_021744.1.
UniGeneiRn.42942.

3D structure databases

ProteinModelPortaliQ63691.
SMRiQ63691. Positions 20-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023977.

Proteomic databases

PaxDbiQ63691.
PRIDEiQ63691.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023977; ENSRNOP00000023977; ENSRNOG00000017819.
GeneIDi60350.
KEGGirno:60350.
UCSCiRGD:620588. rat.

Organism-specific databases

CTDi929.
RGDi620588. Cd14.

Phylogenomic databases

eggNOGiNOG86091.
GeneTreeiENSGT00390000005689.
HOGENOMiHOG000237268.
HOVERGENiHBG005269.
InParanoidiQ63691.
KOiK04391.
OMAiLCPHKFP.
OrthoDBiEOG751NFQ.
PhylomeDBiQ63691.
TreeFamiTF338550.

Enzyme and pathway databases

ReactomeiREACT_279623. Ligand-dependent caspase activation.
REACT_291198. TRAF6 mediated induction of TAK1 complex.
REACT_292892. MyD88-independent TLR3/TLR4 cascade.
REACT_300799. MyD88:Mal cascade initiated on plasma membrane.
REACT_323385. Toll Like Receptor 4 (TLR4) Cascade.
REACT_325185. TRIF-mediated programmed cell death.
REACT_325676. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
REACT_326555. IKK complex recruitment mediated by RIP1.
REACT_344481. Toll Like Receptor TLR6:TLR2 Cascade.
REACT_350755. Transfer of LPS from LBP carrier to CD14.

Miscellaneous databases

NextBioi612011.
PROiQ63691.

Gene expression databases

GenevisibleiQ63691. RN.

Family and domain databases

InterProiIPR001611. Leu-rich_rpt.
IPR016337. Monocyte_diff_Ag_CD14.
[Graphical view]
PIRSFiPIRSF002017. CD14. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CD14 mediate endotoxin induction of nitric oxide synthase in cultured brain glial cells."
    Galea E., Reis D.J., Fox E.S., Xu H., Feinstein D.L.
    J. Neuroimmunol. 64:19-28(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "Primary structure of rat CD14 and characteristics of rat CD14, cytokine, and NO synthase mRNA expression in mononuclear phagocyte system cells in response to LPS."
    Takai N., Kataoka M., Higuchi Y., Matsuura K., Yamamoto S.
    J. Leukoc. Biol. 61:736-744(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Expression of CD14 by hepatocytes: upregulation by cytokines during endotoxemia."
    Liu S., Khemlani L.S., Shapiro R.A., Johnson M.L., Liu K., Geller D.A., Watkins S.C., Goyert S.M., Billiar T.R.
    Infect. Immun. 66:5089-5098(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiCD14_RAT
AccessioniPrimary (citable) accession number: Q63691
Secondary accession number(s): Q6GT04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 2007
Last modified: July 22, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.