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Q63690

- BAX_RAT

UniProt

Q63690 - BAX_RAT

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Protein

Apoptosis regulator BAX

Gene

Bax

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

GO - Molecular functioni

  1. BH3 domain binding Source: UniProtKB
  2. BH domain binding Source: RGD
  3. channel activity Source: UniProtKB
  4. chaperone binding Source: RGD
  5. heat shock protein binding Source: RGD
  6. lipid binding Source: UniProtKB
  7. protein complex binding Source: RGD
  8. protein heterodimerization activity Source: RGD
  9. protein homodimerization activity Source: RefGenome

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  2. activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: UniProtKB
  3. apoptotic mitochondrial changes Source: UniProtKB
  4. apoptotic process Source: RGD
  5. apoptotic signaling pathway Source: UniProtKB
  6. B cell apoptotic process Source: UniProtKB
  7. brain development Source: RGD
  8. cellular respiration Source: RGD
  9. establishment or maintenance of transmembrane electrochemical gradient Source: UniProtKB
  10. extrinsic apoptotic signaling pathway in absence of ligand Source: RefGenome
  11. glial cell apoptotic process Source: RGD
  12. inner mitochondrial membrane organization Source: RGD
  13. intrinsic apoptotic signaling pathway Source: UniProtKB
  14. intrinsic apoptotic signaling pathway in response to DNA damage Source: RefGenome
  15. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
  16. mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
  17. mitochondrial fusion Source: UniProtKB
  18. negative regulation of protein binding Source: UniProtKB
  19. outer mitochondrial membrane organization Source: RGD
  20. ovarian follicle development Source: RGD
  21. positive regulation of apoptotic process Source: UniProtKB
  22. positive regulation of endoplasmic reticulum unfolded protein response Source: UniProtKB
  23. positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
  24. positive regulation of neuron apoptotic process Source: UniProtKB
  25. positive regulation of protein oligomerization Source: UniProtKB
  26. positive regulation of release of cytochrome c from mitochondria Source: UniProtKB
  27. regulation of mitochondrial membrane potential Source: UniProtKB
  28. regulation of protein homodimerization activity Source: UniProtKB
  29. release of cytochrome c from mitochondria Source: RefGenome
  30. release of matrix enzymes from mitochondria Source: UniProtKB
  31. response to cocaine Source: RGD
  32. response to copper ion Source: RGD
  33. response to corticosterone Source: RGD
  34. response to drug Source: RGD
  35. response to toxic substance Source: UniProtKB
  36. retinal cell apoptotic process Source: UniProtKB
  37. transformed cell apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis regulator BAX
Gene namesi
Name:Bax
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2192. Bax.

Subcellular locationi

Isoform Alpha : Mitochondrion outer membrane By similarity; Single-pass membrane protein By similarity. Cytoplasm By similarity
Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane (By similarity).By similarity
Isoform Beta : Cytoplasm By similarity
Isoform Gamma : Cytoplasm By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. BAX complex Source: UniProtKB
  2. Bcl-2 family protein complex Source: UniProtKB
  3. cytoplasm Source: RGD
  4. cytosol Source: RGD
  5. endoplasmic reticulum membrane Source: UniProtKB
  6. mitochondrial membrane Source: RGD
  7. mitochondrial outer membrane Source: RGD
  8. mitochondrial permeability transition pore complex Source: UniProtKB
  9. mitochondrion Source: RGD
  10. nucleus Source: UniProtKB
  11. pore complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Apoptosis regulator BAXPRO_0000143058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ63690.
PRIDEiQ63690.

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues, with highest levels in the testis and ovary.

Gene expression databases

GenevestigatoriQ63690.

Interactioni

Subunit structurei

Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells (By similarity). Interacts with FAIM2/LFG2 (By similarity). Interacts with BOP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Hspd1P630392EBI-822405,EBI-432091

Protein-protein interaction databases

BioGridi246997. 1 interaction.
IntActiQ63690. 3 interactions.
MINTiMINT-1541764.

Structurei

3D structure databases

ProteinModelPortaliQ63690.
SMRiQ63690. Positions 1-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 7315BH3Add
BLAST
Motifi98 – 11821BH1Add
BLAST
Motifi150 – 16516BH2Add
BLAST

Domaini

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.By similarity

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46695.
HOGENOMiHOG000095178.
HOVERGENiHBG003606.
InParanoidiQ63690.
PhylomeDBiQ63690.

Family and domain databases

InterProiIPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF8. PTHR11256:SF8. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform Alpha (identifier: Q63690-1) [UniParc]FASTAAdd to Basket

Also known as: 21 kDa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGSGEQLGG GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP
60 70 80 90 100
PQDASTKKLS ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF
110 120 130 140 150
ADGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLV
160 170 180 190
WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG
Length:192
Mass (Da):21,351
Last modified:November 1, 1997 - v2
Checksum:i7B3CD198D56DF589
GO
Isoform Beta (identifier: Q63690-2)

Sequence is not available
Length:
Mass (Da):
Isoform Gamma (identifier: Q63690-3)

Sequence is not available
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721S → N in AAC26327. (PubMed:8600029)Curated
Sequence conflicti72 – 721S → N in AAA75200. (PubMed:7828536)Curated
Sequence conflicti72 – 721S → N in AAC60700. (PubMed:7828536)Curated
Sequence conflicti76 – 761L → M in AAC52998. (PubMed:8994223)Curated
Sequence conflicti126 – 1261C → Y in AAC52998. (PubMed:8994223)Curated
Sequence conflicti149 – 1491L → F in AAA75200. (PubMed:7828536)Curated
Sequence conflicti149 – 1491L → F in AAC60700. (PubMed:7828536)Curated
Sequence conflicti159 – 1591D → E in AAC26327. (PubMed:8600029)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49729 mRNA. Translation: AAC26327.1.
U59184 mRNA. Translation: AAC52998.1.
U32098 mRNA. Translation: AAA75200.1.
S76511 mRNA. Translation: AAC60700.2.
PIRiI53295.
UniGeneiRn.10668.
Rn.232358.

Genome annotation databases

UCSCiRGD:2192. rat. [Q63690-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49729 mRNA. Translation: AAC26327.1 .
U59184 mRNA. Translation: AAC52998.1 .
U32098 mRNA. Translation: AAA75200.1 .
S76511 mRNA. Translation: AAC60700.2 .
PIRi I53295.
UniGenei Rn.10668.
Rn.232358.

3D structure databases

ProteinModelPortali Q63690.
SMRi Q63690. Positions 1-192.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246997. 1 interaction.
IntActi Q63690. 3 interactions.
MINTi MINT-1541764.

Proteomic databases

PaxDbi Q63690.
PRIDEi Q63690.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2192. rat. [Q63690-1 ]

Organism-specific databases

RGDi 2192. Bax.

Phylogenomic databases

eggNOGi NOG46695.
HOGENOMi HOG000095178.
HOVERGENi HBG003606.
InParanoidi Q63690.
PhylomeDBi Q63690.

Miscellaneous databases

NextBioi 13955757.
PROi Q63690.

Gene expression databases

Genevestigatori Q63690.

Family and domain databases

InterProi IPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view ]
PANTHERi PTHR11256. PTHR11256. 1 hit.
PTHR11256:SF8. PTHR11256:SF8. 1 hit.
Pfami PF00452. Bcl-2. 1 hit.
[Graphical view ]
PRINTSi PR01862. BCL2FAMILY.
PROSITEi PS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The E1B 19K protein blocks apoptosis by interacting with and inhibiting the p53-inducible and death-promoting Bax protein."
    Han J., Sabbatini P., Perez D., Rao L., Modha D., White E.
    Genes Dev. 10:461-477(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of the 3' end of rat bax-alpha and corresponding developmental down-regulation in differentiating primary, cultured oligodendrocytes."
    Madison D.L., Pfeiffer S.E.
    Neurosci. Lett. 220:183-186(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-192.
    Tissue: Brain.
  3. "Expression of members of the Bcl-2 gene family in the immature rat ovary: equine chorionic gonadotropin-mediated inhibition of granulosa cell apoptosis is associated with decreased Bax and constitutive Bcl-2 and Bcl-xlong messenger ribonucleic acid levels."
    Tilly J.L., Tilly K.I., Kenton M.L., Johnson A.L.
    Endocrinology 136:232-241(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-169.
    Strain: Sprague-Dawley.
    Tissue: Ovary.

Entry informationi

Entry nameiBAX_RAT
AccessioniPrimary (citable) accession number: Q63690
Secondary accession number(s): Q62995, Q64383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3