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Protein

Apoptosis regulator BAX

Gene

Bax

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

GO - Molecular functioni

  • BH3 domain binding Source: UniProtKB
  • BH domain binding Source: RGD
  • channel activity Source: UniProtKB
  • chaperone binding Source: RGD
  • heat shock protein binding Source: RGD
  • lipid binding Source: UniProtKB
  • protein complex binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis regulator BAX
Gene namesi
Name:Bax
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2192. Bax.

Subcellular locationi

Isoform Alpha :
  • Mitochondrion outer membrane By similarity; Single-pass membrane protein By similarity
  • Cytoplasm By similarity

  • Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane (By similarity).By similarity
Isoform Beta :
Isoform Gamma :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • BAX complex Source: UniProtKB
  • Bcl-2 family protein complex Source: UniProtKB
  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • endoplasmic reticulum membrane Source: UniProtKB
  • mitochondrial membrane Source: RGD
  • mitochondrial outer membrane Source: RGD
  • mitochondrial permeability transition pore complex Source: UniProtKB
  • mitochondrion Source: RGD
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: RGD
  • pore complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001430581 – 192Apoptosis regulator BAXAdd BLAST192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ63690.
PRIDEiQ63690.

PTM databases

iPTMnetiQ63690.
PhosphoSitePlusiQ63690.

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues, with highest levels in the testis and ovary.

Interactioni

Subunit structurei

Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells (By similarity). Interacts with FAIM2/LFG2 (By similarity). Interacts with BOP (By similarity). Interacts (via a C-terminal 33 residues) with NOL3 (via CARD domain); inhibits BAX activation and translocationand consequently cytochrome c release from mitochondria.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Hspd1P630392EBI-822405,EBI-432091

GO - Molecular functioni

  • BH3 domain binding Source: UniProtKB
  • BH domain binding Source: RGD
  • chaperone binding Source: RGD
  • heat shock protein binding Source: RGD
  • protein complex binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: GO_Central

Protein-protein interaction databases

IntActiQ63690. 3 interactors.
MINTiMINT-1541764.
STRINGi10116.ENSRNOP00000028328.

Structurei

3D structure databases

ProteinModelPortaliQ63690.
SMRiQ63690.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 73BH3Add BLAST15
Motifi98 – 118BH1Add BLAST21
Motifi150 – 165BH2Add BLAST16

Domaini

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.By similarity

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWQJ. Eukaryota.
ENOG4112738. LUCA.
HOGENOMiHOG000095178.
HOVERGENiHBG003606.
InParanoidiQ63690.
PhylomeDBiQ63690.

Family and domain databases

InterProiIPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF44. PTHR11256:SF44. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q63690-1) [UniParc]FASTAAdd to basket
Also known as: 21 kDa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGSGEQLGG GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP
60 70 80 90 100
PQDASTKKLS ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF
110 120 130 140 150
ADGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLV
160 170 180 190
WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG
Length:192
Mass (Da):21,351
Last modified:November 1, 1997 - v2
Checksum:i7B3CD198D56DF589
GO
Isoform Beta (identifier: Q63690-2)
Sequence is not available
Length:
Mass (Da):
Isoform Gamma (identifier: Q63690-3)
Sequence is not available
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72S → N in AAC26327 (PubMed:8600029).Curated1
Sequence conflicti72S → N in AAA75200 (PubMed:7828536).Curated1
Sequence conflicti72S → N in AAC60700 (PubMed:7828536).Curated1
Sequence conflicti76L → M in AAC52998 (PubMed:8994223).Curated1
Sequence conflicti126C → Y in AAC52998 (PubMed:8994223).Curated1
Sequence conflicti149L → F in AAA75200 (PubMed:7828536).Curated1
Sequence conflicti149L → F in AAC60700 (PubMed:7828536).Curated1
Sequence conflicti159D → E in AAC26327 (PubMed:8600029).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49729 mRNA. Translation: AAC26327.1.
U59184 mRNA. Translation: AAC52998.1.
U32098 mRNA. Translation: AAA75200.1.
S76511 mRNA. Translation: AAC60700.2.
PIRiI53295.
UniGeneiRn.10668.
Rn.232358.

Genome annotation databases

UCSCiRGD:2192. rat. [Q63690-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49729 mRNA. Translation: AAC26327.1.
U59184 mRNA. Translation: AAC52998.1.
U32098 mRNA. Translation: AAA75200.1.
S76511 mRNA. Translation: AAC60700.2.
PIRiI53295.
UniGeneiRn.10668.
Rn.232358.

3D structure databases

ProteinModelPortaliQ63690.
SMRiQ63690.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63690. 3 interactors.
MINTiMINT-1541764.
STRINGi10116.ENSRNOP00000028328.

PTM databases

iPTMnetiQ63690.
PhosphoSitePlusiQ63690.

Proteomic databases

PaxDbiQ63690.
PRIDEiQ63690.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2192. rat. [Q63690-1]

Organism-specific databases

RGDi2192. Bax.

Phylogenomic databases

eggNOGiENOG410IWQJ. Eukaryota.
ENOG4112738. LUCA.
HOGENOMiHOG000095178.
HOVERGENiHBG003606.
InParanoidiQ63690.
PhylomeDBiQ63690.

Miscellaneous databases

PROiQ63690.

Family and domain databases

InterProiIPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF44. PTHR11256:SF44. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAX_RAT
AccessioniPrimary (citable) accession number: Q63690
Secondary accession number(s): Q62995, Q64383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.