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Q63690

- BAX_RAT

UniProt

Q63690 - BAX_RAT

Protein

Apoptosis regulator BAX

Gene

Bax

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

    GO - Molecular functioni

    1. BH3 domain binding Source: UniProtKB
    2. BH domain binding Source: RGD
    3. channel activity Source: UniProtKB
    4. chaperone binding Source: RGD
    5. heat shock protein binding Source: RGD
    6. lipid binding Source: UniProtKB
    7. protein binding Source: IntAct
    8. protein complex binding Source: RGD
    9. protein heterodimerization activity Source: RGD
    10. protein homodimerization activity Source: RefGenome

    GO - Biological processi

    1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    2. activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: UniProtKB
    3. apoptotic mitochondrial changes Source: UniProtKB
    4. apoptotic process Source: RGD
    5. apoptotic signaling pathway Source: UniProtKB
    6. B cell apoptotic process Source: UniProtKB
    7. brain development Source: RGD
    8. cellular respiration Source: RGD
    9. establishment or maintenance of transmembrane electrochemical gradient Source: UniProtKB
    10. extrinsic apoptotic signaling pathway in absence of ligand Source: RefGenome
    11. glial cell apoptotic process Source: RGD
    12. inner mitochondrial membrane organization Source: RGD
    13. intrinsic apoptotic signaling pathway Source: UniProtKB
    14. intrinsic apoptotic signaling pathway in response to DNA damage Source: RefGenome
    15. intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
    16. mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
    17. mitochondrial fusion Source: UniProtKB
    18. negative regulation of protein binding Source: UniProtKB
    19. outer mitochondrial membrane organization Source: RGD
    20. ovarian follicle development Source: RGD
    21. positive regulation of apoptotic process Source: UniProtKB
    22. positive regulation of endoplasmic reticulum unfolded protein response Source: UniProtKB
    23. positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
    24. positive regulation of neuron apoptotic process Source: UniProtKB
    25. positive regulation of protein oligomerization Source: UniProtKB
    26. positive regulation of release of cytochrome c from mitochondria Source: UniProtKB
    27. regulation of mitochondrial membrane potential Source: UniProtKB
    28. regulation of protein homodimerization activity Source: UniProtKB
    29. release of cytochrome c from mitochondria Source: RefGenome
    30. release of matrix enzymes from mitochondria Source: UniProtKB
    31. response to cocaine Source: RGD
    32. response to copper ion Source: RGD
    33. response to corticosterone Source: RGD
    34. response to drug Source: RGD
    35. response to toxic substance Source: UniProtKB
    36. retinal cell apoptotic process Source: UniProtKB
    37. transformed cell apoptotic process Source: UniProtKB

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apoptosis regulator BAX
    Gene namesi
    Name:Bax
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2192. Bax.

    Subcellular locationi

    Isoform Alpha : Mitochondrion outer membrane By similarity; Single-pass membrane protein By similarity. Cytoplasm By similarity
    Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane By similarity.By similarity
    Isoform Beta : Cytoplasm By similarity
    Isoform Gamma : Cytoplasm By similarity

    GO - Cellular componenti

    1. BAX complex Source: UniProtKB
    2. Bcl-2 family protein complex Source: UniProtKB
    3. cytoplasm Source: RGD
    4. cytosol Source: RGD
    5. endoplasmic reticulum membrane Source: UniProtKB
    6. mitochondrial membrane Source: RGD
    7. mitochondrial outer membrane Source: RGD
    8. mitochondrial permeability transition pore complex Source: UniProtKB
    9. mitochondrion Source: RGD
    10. nucleus Source: UniProtKB
    11. pore complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 192192Apoptosis regulator BAXPRO_0000143058Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ63690.
    PRIDEiQ63690.

    Expressioni

    Tissue specificityi

    Expressed in a wide variety of tissues, with highest levels in the testis and ovary.

    Gene expression databases

    GenevestigatoriQ63690.

    Interactioni

    Subunit structurei

    Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells By similarity. Interacts with FAIM2/LFG2 By similarity. Interacts with BOP By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Hspd1P630392EBI-822405,EBI-432091

    Protein-protein interaction databases

    BioGridi246997. 1 interaction.
    IntActiQ63690. 3 interactions.
    MINTiMINT-1541764.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63690.
    SMRiQ63690. Positions 1-192.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei172 – 19221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 7315BH3Add
    BLAST
    Motifi98 – 11821BH1Add
    BLAST
    Motifi150 – 16516BH2Add
    BLAST

    Domaini

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.By similarity

    Sequence similaritiesi

    Belongs to the Bcl-2 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46695.
    HOGENOMiHOG000095178.
    HOVERGENiHBG003606.
    InParanoidiQ63690.
    PhylomeDBiQ63690.

    Family and domain databases

    InterProiIPR026304. BAX.
    IPR002475. Bcl2-like.
    IPR020717. Bcl2_BH1_motif_CS.
    IPR020726. Bcl2_BH2_motif_CS.
    IPR020728. Bcl2_BH3_motif_CS.
    IPR026298. Blc2_fam.
    [Graphical view]
    PANTHERiPTHR11256. PTHR11256. 1 hit.
    PTHR11256:SF8. PTHR11256:SF8. 1 hit.
    PfamiPF00452. Bcl-2. 1 hit.
    [Graphical view]
    PRINTSiPR01862. BCL2FAMILY.
    PROSITEiPS50062. BCL2_FAMILY. 1 hit.
    PS01080. BH1. 1 hit.
    PS01258. BH2. 1 hit.
    PS01259. BH3. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform Alpha (identifier: Q63690-1) [UniParc]FASTAAdd to Basket

    Also known as: 21 kDa

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDGSGEQLGG GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP    50
    PQDASTKKLS ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF 100
    ADGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLV 150
    WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG 192
    Length:192
    Mass (Da):21,351
    Last modified:November 1, 1997 - v2
    Checksum:i7B3CD198D56DF589
    GO
    Isoform Beta (identifier: Q63690-2)

    Sequence is not available
    Length:
    Mass (Da):
    Isoform Gamma (identifier: Q63690-3)

    Sequence is not available
    Length:
    Mass (Da):

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 721S → N in AAC26327. (PubMed:8600029)Curated
    Sequence conflicti72 – 721S → N in AAA75200. (PubMed:7828536)Curated
    Sequence conflicti72 – 721S → N in AAC60700. (PubMed:7828536)Curated
    Sequence conflicti76 – 761L → M in AAC52998. (PubMed:8994223)Curated
    Sequence conflicti126 – 1261C → Y in AAC52998. (PubMed:8994223)Curated
    Sequence conflicti149 – 1491L → F in AAA75200. (PubMed:7828536)Curated
    Sequence conflicti149 – 1491L → F in AAC60700. (PubMed:7828536)Curated
    Sequence conflicti159 – 1591D → E in AAC26327. (PubMed:8600029)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U49729 mRNA. Translation: AAC26327.1.
    U59184 mRNA. Translation: AAC52998.1.
    U32098 mRNA. Translation: AAA75200.1.
    S76511 mRNA. Translation: AAC60700.2.
    PIRiI53295.
    UniGeneiRn.10668.
    Rn.232358.

    Genome annotation databases

    UCSCiRGD:2192. rat. [Q63690-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U49729 mRNA. Translation: AAC26327.1 .
    U59184 mRNA. Translation: AAC52998.1 .
    U32098 mRNA. Translation: AAA75200.1 .
    S76511 mRNA. Translation: AAC60700.2 .
    PIRi I53295.
    UniGenei Rn.10668.
    Rn.232358.

    3D structure databases

    ProteinModelPortali Q63690.
    SMRi Q63690. Positions 1-192.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246997. 1 interaction.
    IntActi Q63690. 3 interactions.
    MINTi MINT-1541764.

    Proteomic databases

    PaxDbi Q63690.
    PRIDEi Q63690.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2192. rat. [Q63690-1 ]

    Organism-specific databases

    RGDi 2192. Bax.

    Phylogenomic databases

    eggNOGi NOG46695.
    HOGENOMi HOG000095178.
    HOVERGENi HBG003606.
    InParanoidi Q63690.
    PhylomeDBi Q63690.

    Miscellaneous databases

    NextBioi 13955757.
    PROi Q63690.

    Gene expression databases

    Genevestigatori Q63690.

    Family and domain databases

    InterProi IPR026304. BAX.
    IPR002475. Bcl2-like.
    IPR020717. Bcl2_BH1_motif_CS.
    IPR020726. Bcl2_BH2_motif_CS.
    IPR020728. Bcl2_BH3_motif_CS.
    IPR026298. Blc2_fam.
    [Graphical view ]
    PANTHERi PTHR11256. PTHR11256. 1 hit.
    PTHR11256:SF8. PTHR11256:SF8. 1 hit.
    Pfami PF00452. Bcl-2. 1 hit.
    [Graphical view ]
    PRINTSi PR01862. BCL2FAMILY.
    PROSITEi PS50062. BCL2_FAMILY. 1 hit.
    PS01080. BH1. 1 hit.
    PS01258. BH2. 1 hit.
    PS01259. BH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The E1B 19K protein blocks apoptosis by interacting with and inhibiting the p53-inducible and death-promoting Bax protein."
      Han J., Sabbatini P., Perez D., Rao L., Modha D., White E.
      Genes Dev. 10:461-477(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning of the 3' end of rat bax-alpha and corresponding developmental down-regulation in differentiating primary, cultured oligodendrocytes."
      Madison D.L., Pfeiffer S.E.
      Neurosci. Lett. 220:183-186(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-192.
      Tissue: Brain.
    3. "Expression of members of the Bcl-2 gene family in the immature rat ovary: equine chorionic gonadotropin-mediated inhibition of granulosa cell apoptosis is associated with decreased Bax and constitutive Bcl-2 and Bcl-xlong messenger ribonucleic acid levels."
      Tilly J.L., Tilly K.I., Kenton M.L., Johnson A.L.
      Endocrinology 136:232-241(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-169.
      Strain: Sprague-Dawley.
      Tissue: Ovary.

    Entry informationi

    Entry nameiBAX_RAT
    AccessioniPrimary (citable) accession number: Q63690
    Secondary accession number(s): Q62995, Q64383
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3