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Q63690 (BAX_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apoptosis regulator BAX
Gene names
Name:Bax
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length192 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Subunit structure

Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L) BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells By similarity. Interacts with FAIM2/LFG2 By similarity. Interacts with BOP By similarity.

Subcellular location

Isoform Alpha: Mitochondrion outer membrane; Single-pass membrane protein By similarity. Cytoplasm By similarity. Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane By similarity.

Isoform Beta: Cytoplasm By similarity.

Isoform Gamma: Cytoplasm By similarity.

Tissue specificity

Expressed in a wide variety of tissues, with highest levels in the testis and ovary.

Domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family By similarity.

Sequence similarities

Belongs to the Bcl-2 family.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentCytoplasm
Membrane
Mitochondrion
Mitochondrion outer membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

activation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c

Inferred from sequence or structural similarity. Source: UniProtKB

apoptotic mitochondrial changes

Inferred from sequence or structural similarity. Source: UniProtKB

apoptotic process

Inferred from mutant phenotype PubMed 16084019PubMed 18817839. Source: RGD

apoptotic signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

brain development

Inferred from expression pattern PubMed 18058945. Source: RGD

cellular respiration

Inferred from direct assay PubMed 12460673. Source: RGD

establishment or maintenance of transmembrane electrochemical gradient

Inferred from sequence or structural similarity. Source: UniProtKB

extrinsic apoptotic signaling pathway in absence of ligand

Inferred from Biological aspect of Ancestor. Source: RefGenome

glial cell apoptotic process

Inferred from expression pattern PubMed 18931364. Source: RGD

inner mitochondrial membrane organization

Inferred from direct assay PubMed 12460673. Source: RGD

intrinsic apoptotic signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

intrinsic apoptotic signaling pathway in response to DNA damage

Inferred from Biological aspect of Ancestor. Source: RefGenome

intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrial fragmentation involved in apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrial fusion

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of protein binding

Inferred from sequence or structural similarity. Source: UniProtKB

outer mitochondrial membrane organization

Inferred from direct assay PubMed 12460673. Source: RGD

ovarian follicle development

Inferred from expression pattern PubMed 17570453. Source: RGD

positive regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of endoplasmic reticulum unfolded protein response

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of intrinsic apoptotic signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of neuron apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of protein oligomerization

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of release of cytochrome c from mitochondria

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of mitochondrial membrane potential

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

release of cytochrome c from mitochondria

Inferred from Biological aspect of Ancestor. Source: RefGenome

release of matrix enzymes from mitochondria

Inferred from sequence or structural similarity. Source: UniProtKB

response to cocaine

Inferred from expression pattern PubMed 17084983. Source: RGD

response to copper ion

Inferred from expression pattern PubMed 18289470. Source: RGD

response to corticosterone

Inferred from expression pattern PubMed 17562391. Source: RGD

response to drug

Inferred from expression pattern PubMed 17784886. Source: RGD

response to toxic substance

Inferred from sequence or structural similarity. Source: UniProtKB

retinal cell apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

transformed cell apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentBAX complex

Inferred from sequence or structural similarity. Source: UniProtKB

Bcl-2 family protein complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from direct assay PubMed 15341589PubMed 16321138. Source: RGD

cytosol

Inferred from direct assay PubMed 15902200PubMed 16084019PubMed 16579988. Source: RGD

endoplasmic reticulum membrane

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrial membrane

Inferred from direct assay PubMed 16084019. Source: RGD

mitochondrial outer membrane

Inferred from direct assay PubMed 15044178. Source: RGD

mitochondrial permeability transition pore complex

Inferred from sequence or structural similarity. Source: UniProtKB

mitochondrion

Inferred from direct assay PubMed 15341589PubMed 15902200PubMed 16321138PubMed 16579988. Source: RGD

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

pore complex

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionBH domain binding

Inferred from physical interaction PubMed 9356461. Source: RGD

BH3 domain binding

Inferred from sequence or structural similarity. Source: UniProtKB

channel activity

Inferred from sequence or structural similarity. Source: UniProtKB

chaperone binding

Inferred from physical interaction PubMed 12070120. Source: RGD

heat shock protein binding

Inferred from physical interaction PubMed 12070120PubMed 16579988. Source: RGD

lipid binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein complex binding

Inferred from physical interaction PubMed 21561597. Source: RGD

protein heterodimerization activity

Inferred from physical interaction PubMed 16579988PubMed 9356461. Source: RGD

protein homodimerization activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Hspd1P630392EBI-822405,EBI-432091

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Select]
Isoform Alpha (identifier: Q63690-1)

Also known as: 21 kDa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: Q63690-2)

The sequence of this isoform is not available.
Isoform Gamma (identifier: Q63690-3)

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 192192Apoptosis regulator BAX
PRO_0000143058

Regions

Transmembrane172 – 19221Helical; Potential
Motif59 – 7315BH3
Motif98 – 11821BH1
Motif150 – 16516BH2

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Experimental info

Sequence conflict721S → N in AAC26327. Ref.1
Sequence conflict721S → N in AAA75200. Ref.3
Sequence conflict721S → N in AAC60700. Ref.3
Sequence conflict761L → M in AAC52998. Ref.2
Sequence conflict1261C → Y in AAC52998. Ref.2
Sequence conflict1491L → F in AAA75200. Ref.3
Sequence conflict1491L → F in AAC60700. Ref.3
Sequence conflict1591D → E in AAC26327. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha (21 kDa) [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 7B3CD198D56DF589

FASTA19221,351
        10         20         30         40         50         60 
MDGSGEQLGG GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP PQDASTKKLS 

        70         80         90        100        110        120 
ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF ADGNFNWGRV VALFYFASKL 

       130        140        150        160        170        180 
VLKALCTKVP ELIRTIMGWT LDFLRERLLV WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV 

       190 
LTASLTIWKK MG 

« Hide

Isoform Beta (Sequence not available).
Isoform Gamma (Sequence not available).

References

[1]"The E1B 19K protein blocks apoptosis by interacting with and inhibiting the p53-inducible and death-promoting Bax protein."
Han J., Sabbatini P., Perez D., Rao L., Modha D., White E.
Genes Dev. 10:461-477(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning of the 3' end of rat bax-alpha and corresponding developmental down-regulation in differentiating primary, cultured oligodendrocytes."
Madison D.L., Pfeiffer S.E.
Neurosci. Lett. 220:183-186(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-192.
Tissue: Brain.
[3]"Expression of members of the Bcl-2 gene family in the immature rat ovary: equine chorionic gonadotropin-mediated inhibition of granulosa cell apoptosis is associated with decreased Bax and constitutive Bcl-2 and Bcl-xlong messenger ribonucleic acid levels."
Tilly J.L., Tilly K.I., Kenton M.L., Johnson A.L.
Endocrinology 136:232-241(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-169.
Strain: Sprague-Dawley.
Tissue: Ovary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U49729 mRNA. Translation: AAC26327.1.
U59184 mRNA. Translation: AAC52998.1.
U32098 mRNA. Translation: AAA75200.1.
S76511 mRNA. Translation: AAC60700.2.
PIRI53295.
UniGeneRn.10668.
Rn.232358.

3D structure databases

ProteinModelPortalQ63690.
SMRQ63690. Positions 1-192.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246997. 1 interaction.
IntActQ63690. 3 interactions.
MINTMINT-1541764.

Proteomic databases

PaxDbQ63690.
PRIDEQ63690.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:2192. rat. [Q63690-1]

Organism-specific databases

RGD2192. Bax.

Phylogenomic databases

eggNOGNOG46695.
HOGENOMHOG000095178.
HOVERGENHBG003606.
InParanoidQ63690.
PhylomeDBQ63690.

Gene expression databases

GenevestigatorQ63690.

Family and domain databases

InterProIPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF9. PTHR11256:SF9. 1 hit.
PfamPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSPR01862. BCL2FAMILY.
PROSITEPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio13955757.
PROQ63690.

Entry information

Entry nameBAX_RAT
AccessionPrimary (citable) accession number: Q63690
Secondary accession number(s): Q62995, Q64383
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families