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Protein

Apoptosis regulator BAX

Gene

Bax

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

GO - Molecular functioni

  • BH3 domain binding Source: UniProtKB
  • BH domain binding Source: RGD
  • channel activity Source: UniProtKB
  • chaperone binding Source: RGD
  • heat shock protein binding Source: RGD
  • lipid binding Source: UniProtKB
  • protein complex binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis regulator BAX
Gene namesi
Name:Bax
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2192. Bax.

Subcellular locationi

Isoform Alpha :
  • Mitochondrion outer membrane By similarity; Single-pass membrane protein By similarity
  • Cytoplasm By similarity

  • Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane (By similarity).By similarity
Isoform Beta :
Isoform Gamma :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • BAX complex Source: UniProtKB
  • Bcl-2 family protein complex Source: UniProtKB
  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • endoplasmic reticulum membrane Source: UniProtKB
  • mitochondrial membrane Source: RGD
  • mitochondrial outer membrane Source: RGD
  • mitochondrial permeability transition pore complex Source: UniProtKB
  • mitochondrion Source: RGD
  • nucleus Source: UniProtKB
  • pore complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Apoptosis regulator BAXPRO_0000143058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ63690.
PRIDEiQ63690.

Expressioni

Tissue specificityi

Expressed in a wide variety of tissues, with highest levels in the testis and ovary.

Gene expression databases

GenevisibleiQ63690. RN.

Interactioni

Subunit structurei

Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells (By similarity). Interacts with FAIM2/LFG2 (By similarity). Interacts with BOP (By similarity). Interacts (via a C-terminal 33 residues) with NOL3 (via CARD domain); inhibits BAX activation and translocationand consequently cytochrome c release from mitochondria.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Hspd1P630392EBI-822405,EBI-432091

Protein-protein interaction databases

IntActiQ63690. 3 interactions.
MINTiMINT-1541764.
STRINGi10116.ENSRNOP00000028328.

Structurei

3D structure databases

ProteinModelPortaliQ63690.
SMRiQ63690. Positions 1-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 7315BH3Add
BLAST
Motifi98 – 11821BH1Add
BLAST
Motifi150 – 16516BH2Add
BLAST

Domaini

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.By similarity

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46695.
HOGENOMiHOG000095178.
HOVERGENiHBG003606.
InParanoidiQ63690.
PhylomeDBiQ63690.

Family and domain databases

InterProiIPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF8. PTHR11256:SF8. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q63690-1) [UniParc]FASTAAdd to basket

Also known as: 21 kDa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGSGEQLGG GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP
60 70 80 90 100
PQDASTKKLS ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF
110 120 130 140 150
ADGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLV
160 170 180 190
WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG
Length:192
Mass (Da):21,351
Last modified:November 1, 1997 - v2
Checksum:i7B3CD198D56DF589
GO
Isoform Beta (identifier: Q63690-2)

Sequence is not available
Length:
Mass (Da):
Isoform Gamma (identifier: Q63690-3)

Sequence is not available
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721S → N in AAC26327 (PubMed:8600029).Curated
Sequence conflicti72 – 721S → N in AAA75200 (PubMed:7828536).Curated
Sequence conflicti72 – 721S → N in AAC60700 (PubMed:7828536).Curated
Sequence conflicti76 – 761L → M in AAC52998 (PubMed:8994223).Curated
Sequence conflicti126 – 1261C → Y in AAC52998 (PubMed:8994223).Curated
Sequence conflicti149 – 1491L → F in AAA75200 (PubMed:7828536).Curated
Sequence conflicti149 – 1491L → F in AAC60700 (PubMed:7828536).Curated
Sequence conflicti159 – 1591D → E in AAC26327 (PubMed:8600029).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49729 mRNA. Translation: AAC26327.1.
U59184 mRNA. Translation: AAC52998.1.
U32098 mRNA. Translation: AAA75200.1.
S76511 mRNA. Translation: AAC60700.2.
PIRiI53295.
UniGeneiRn.10668.
Rn.232358.

Genome annotation databases

UCSCiRGD:2192. rat. [Q63690-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49729 mRNA. Translation: AAC26327.1.
U59184 mRNA. Translation: AAC52998.1.
U32098 mRNA. Translation: AAA75200.1.
S76511 mRNA. Translation: AAC60700.2.
PIRiI53295.
UniGeneiRn.10668.
Rn.232358.

3D structure databases

ProteinModelPortaliQ63690.
SMRiQ63690. Positions 1-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63690. 3 interactions.
MINTiMINT-1541764.
STRINGi10116.ENSRNOP00000028328.

Proteomic databases

PaxDbiQ63690.
PRIDEiQ63690.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2192. rat. [Q63690-1]

Organism-specific databases

RGDi2192. Bax.

Phylogenomic databases

eggNOGiNOG46695.
HOGENOMiHOG000095178.
HOVERGENiHBG003606.
InParanoidiQ63690.
PhylomeDBiQ63690.

Miscellaneous databases

NextBioi13955757.
PROiQ63690.

Gene expression databases

GenevisibleiQ63690. RN.

Family and domain databases

InterProiIPR026304. BAX.
IPR002475. Bcl2-like.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
IPR020728. Bcl2_BH3_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PANTHERiPTHR11256. PTHR11256. 1 hit.
PTHR11256:SF8. PTHR11256:SF8. 1 hit.
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PRINTSiPR01862. BCL2FAMILY.
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
PS01259. BH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The E1B 19K protein blocks apoptosis by interacting with and inhibiting the p53-inducible and death-promoting Bax protein."
    Han J., Sabbatini P., Perez D., Rao L., Modha D., White E.
    Genes Dev. 10:461-477(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of the 3' end of rat bax-alpha and corresponding developmental down-regulation in differentiating primary, cultured oligodendrocytes."
    Madison D.L., Pfeiffer S.E.
    Neurosci. Lett. 220:183-186(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-192.
    Tissue: Brain.
  3. "Expression of members of the Bcl-2 gene family in the immature rat ovary: equine chorionic gonadotropin-mediated inhibition of granulosa cell apoptosis is associated with decreased Bax and constitutive Bcl-2 and Bcl-xlong messenger ribonucleic acid levels."
    Tilly J.L., Tilly K.I., Kenton M.L., Johnson A.L.
    Endocrinology 136:232-241(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-169.
    Strain: Sprague-Dawley.
    Tissue: Ovary.
  4. "Inhibition of both the extrinsic and intrinsic death pathways through nonhomotypic death-fold interactions."
    Nam Y.J., Mani K., Ashton A.W., Peng C.F., Krishnamurthy B., Hayakawa Y., Lee P., Korsmeyer S.J., Kitsis R.N.
    Mol. Cell 15:901-912(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOL3.

Entry informationi

Entry nameiBAX_RAT
AccessioniPrimary (citable) accession number: Q63690
Secondary accession number(s): Q62995, Q64383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.