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Protein

25-hydroxycholesterol 7-alpha-hydroxylase

Gene

Cyp7b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxysterol 7alpha-hydroxylase that mediates formation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) from 25-hydroxycholesterol (By similarity). Plays a key role in cell positioning and movement in lymphoid tissues: 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (By similarity).By similarity

Catalytic activityi

Cholest-5-ene-3-beta,25-diol + NADPH + O2 = cholest-5-ene-3-beta,7-alpha,25-triol + NADP+ + H2O.
Cholest-5-ene-3-beta,27-diol + NADPH + O2 = cholest-5-ene-3-beta,7-alpha,27-triol + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi: bile acid biosynthesis

This protein is involved in the pathway bile acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway bile acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi354 – 3541Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

  • bile acid biosynthetic process Source: RGD
  • cholesterol metabolic process Source: RGD
  • circadian rhythm Source: RGD
  • memory Source: RGD
  • response to cAMP Source: RGD
  • response to drug Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00221.

Names & Taxonomyi

Protein namesi
Recommended name:
25-hydroxycholesterol 7-alpha-hydroxylase (EC:1.14.13.100)
Alternative name(s):
Cytochrome P450 7B1
Hippocampal transcript 1 protein
Short name:
HCT-1
Oxysterol 7-alpha-hydroxylase
Gene namesi
Name:Cyp7b1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2483. Cyp7b1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 414›41425-hydroxycholesterol 7-alpha-hydroxylasePRO_0000051908Add
BLAST

Proteomic databases

PaxDbiQ63688.
PRIDEiQ63688.

PTM databases

PhosphoSiteiQ63688.

Expressioni

Tissue specificityi

Highly expressed in brain; also expressed in liver and kidney.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013116.

Structurei

3D structure databases

ProteinModelPortaliQ63688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiQ63688.
KOiK07430.
PhylomeDBiQ63688.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q63688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ALEYQYVMKN PKQLSFEKFS RRLSAKAFSV KKLLTNDDLS NDIHRGYLLL
60 70 80 90 100
QGKSLDGLLE TMIQEVKEIF ESRLLKLTDW NTARVFDFCS SLVFEITFTT
110 120 130 140 150
IYGKILAANK KQIISELRDD FLKFDDHFPY LVSDIPIQLL RNAEFMQKKI
160 170 180 190 200
IKCLTPEKVA QMQRRSEIVQ ERQEMLKKYY GHEEFEIGAH HLGLLWASLA
210 220 230 240 250
NTIPAMFWAM YYLLQHPEAM EVLRDEIDSF LQSTGQKKGP GISVHFTREQ
260 270 280 290 300
LDSLVCLESA ILEVLRLCSY SSIIREVQED MDFSSESRSY RLRKGDFVAV
310 320 330 340 350
FPPMIHNDPE VFDAPKDFRF DRFVEDGKKK TTFFKGGKKL KSYIIPFGLG
360 370 380 390 400
TSKCPGRYFA INEMKLLVII LLTYFDLEVI DTKPIGLNHS RMFLGIQHPD
410
SDISFRYKAK SWRS
Length:414
Mass (Da):48,227
Last modified:November 1, 1996 - v1
Checksum:iF2111BF6A1575BD2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36992 mRNA. Translation: AAA92616.1.
RefSeqiNP_062011.1. NM_019138.1.
UniGeneiRn.53969.

Genome annotation databases

GeneIDi25429.
KEGGirno:25429.
UCSCiRGD:2483. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36992 mRNA. Translation: AAA92616.1.
RefSeqiNP_062011.1. NM_019138.1.
UniGeneiRn.53969.

3D structure databases

ProteinModelPortaliQ63688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013116.

PTM databases

PhosphoSiteiQ63688.

Proteomic databases

PaxDbiQ63688.
PRIDEiQ63688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25429.
KEGGirno:25429.
UCSCiRGD:2483. rat.

Organism-specific databases

CTDi9420.
RGDi2483. Cyp7b1.

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiQ63688.
KOiK07430.
PhylomeDBiQ63688.

Enzyme and pathway databases

UniPathwayiUPA00221.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCP7B1_RAT
AccessioniPrimary (citable) accession number: Q63688
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.