Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q63686

- CDK16_RAT

UniProt

Q63686 - CDK16_RAT

Protein

Cyclin-dependent kinase 16

Gene

Cdk16

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Can phosphorylate CCNY at 'Ser-336' (in vitro) Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Regulates GH1 release by brain neurons. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development By similarity. Phosphorylates NSF, and thereby regulates NSF oligomerization.By similarity1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei194 – 1941ATPPROSITE-ProRule annotation
    Active sitei286 – 2861Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi171 – 1799ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. exocytosis Source: UniProtKB
    2. growth hormone secretion Source: UniProtKB
    3. neuron projection development Source: UniProtKB
    4. regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
    5. spermatogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 16 (EC:2.7.11.22)
    Alternative name(s):
    Cell division protein kinase 16
    PCTAIRE-motif protein kinase 1
    Serine/threonine-protein kinase PCTAIRE-1
    Gene namesi
    Name:Cdk16
    Synonyms:Pctaire1, Pctk1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620584. Cdk16.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmic vesiclesecretory vesicle 1 Publication. Cell junctionsynapsesynaptosome 1 Publication
    Note: Colocalizes with insulin in pancreas islets.By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
    3. neuron projection Source: UniProtKB-SubCell
    4. synaptic vesicle Source: UniProtKB
    5. transport vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Cytoplasmic vesicle, Synapse, Synaptosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 496496Cyclin-dependent kinase 16PRO_0000086486Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei12 – 121Phosphoserine; by BRSK2By similarity
    Modified residuei78 – 781PhosphoserineBy similarity
    Modified residuei82 – 821PhosphoserineBy similarity
    Modified residuei95 – 951Phosphoserine; by CDK5By similarity
    Modified residuei119 – 1191PhosphoserineBy similarity
    Modified residuei138 – 1381PhosphoserineBy similarity
    Modified residuei153 – 1531PhosphoserineBy similarity
    Modified residuei155 – 1551PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-153 inhibits kinase activity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ63686.
    PRIDEiQ63686.

    PTM databases

    PhosphoSiteiQ63686.

    Expressioni

    Tissue specificityi

    Detected in brain (at protein level). Isoform 1 and isoform 2 are expressed in brain, kidney, lung, heart and lens fiber.2 Publications

    Gene expression databases

    GenevestigatoriQ63686.

    Interactioni

    Subunit structurei

    Interacts with BRSK2. Interacts with CCNY; this increases the CDK16 kinase activity By similarity. Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with NSF. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1.By similarity2 Publications

    Protein-protein interaction databases

    IntActiQ63686. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63686.
    SMRiQ63686. Positions 173-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini165 – 446282Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiQ63686.
    KOiK08820.
    PhylomeDBiQ63686.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q63686-1) [UniParc]FASTAAdd to Basket

    Also known as: PCTAIRE-1a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDRMKKIKRQ LSMTLRGGRG IDKTNGVPEQ IGLDESGGGG GMTLGEAPTR    50
    VAPGELRSIR GPLSSAPEIV HEDMKMGSDG ESDQASATSS DEVQSPVRVR 100
    MRNHPPRKIS TEDINKCLSL PADIRLPEGY LEKLTLNSPI GDKPLSRRLR 150
    PVSLSEIGFG KLETYIKLDK LGEGTYATVY KGKSKLTDNL VALKEIRLEH 200
    EEGAPCTAIR EVSLLKDLKH ANIVTLHDII HTEKSLTLVF EYLDKDLKQY 250
    LDDCGNVINM HNVKLFLFQL LRGLAYCHRQ KVLHRDLKPQ NLLINERGEL 300
    KLADFGLAYA KSIPTKTYSN EVVTLWYRPP DILLGSTDYS GQIDMWGVGC 350
    IFYEMATGRP LFPGSTVEEQ LHFIFRILGT PTEDTWPGIL SNEEFRTYNY 400
    PKYRAEALLR HAPRLECDGA DLLTKLLQFE GRNRISAEDA MKHPFFLSLG 450
    ERIHKLPDTT SIFALKEVQL QKEANIRSTS MPDSGRPAFR VVDTEF 496
    Length:496
    Mass (Da):55,765
    Last modified:November 1, 1996 - v1
    Checksum:i8E77DB7551A84329
    GO
    Isoform 2 (identifier: Q63686-2) [UniParc]FASTAAdd to Basket

    Also known as: PCTAIRE-1b

    The sequence of this isoform differs from the canonical sequence as follows:
         1-64: MDRMKKIKRQ...ELRSIRGPLS → MYTNGYDEEIYYIGGKRVFLTPKAWPFPH

    Show »
    Length:461
    Mass (Da):52,523
    Checksum:iF543D3C9615AF987
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6464MDRMK…RGPLS → MYTNGYDEEIYYIGGKRVFL TPKAWPFPH in isoform 2. 1 PublicationVSP_010639Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36444 mRNA. Translation: AAC52912.1.
    U36444 mRNA. Translation: AAC52913.1.
    PIRiJC5110.
    JC5111.
    RefSeqiNP_112339.1. NM_031077.1. [Q63686-2]
    UniGeneiRn.16871.

    Genome annotation databases

    GeneIDi81741.
    KEGGirno:81741.
    UCSCiRGD:620584. rat. [Q63686-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36444 mRNA. Translation: AAC52912.1 .
    U36444 mRNA. Translation: AAC52913.1 .
    PIRi JC5110.
    JC5111.
    RefSeqi NP_112339.1. NM_031077.1. [Q63686-2 ]
    UniGenei Rn.16871.

    3D structure databases

    ProteinModelPortali Q63686.
    SMRi Q63686. Positions 173-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q63686. 1 interaction.

    PTM databases

    PhosphoSitei Q63686.

    Proteomic databases

    PaxDbi Q63686.
    PRIDEi Q63686.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 81741.
    KEGGi rno:81741.
    UCSCi RGD:620584. rat. [Q63686-1 ]

    Organism-specific databases

    CTDi 5127.
    RGDi 620584. Cdk16.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi Q63686.
    KOi K08820.
    PhylomeDBi Q63686.

    Miscellaneous databases

    NextBioi 615454.
    PROi Q63686.

    Gene expression databases

    Genevestigatori Q63686.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of alternatively spliced PCTAIRE-1 mRNA in PC12 cells and neonatal rat brain."
      Gao C.Y., Chauthaiwale V.M., Rampalli A.M., Zelenka P.S.
      Gene 176:243-247(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
      Strain: New England Deaconess Hospital.
      Tissue: Adrenal medulla.
    2. "Identification of tudor repeat associator with PCTAIRE 2 (Trap). A novel protein that interacts with the N-terminal domain of PCTAIRE 2 in rat brain."
      Hirose T., Kawabuchi M., Tamaru T., Okumura N., Nagai K., Okada M.
      Eur. J. Biochem. 267:2113-2121(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH CDK17 AND TDRD7.
    3. "Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis."
      Liu Y., Cheng K., Gong K., Fu A.K., Ip N.Y.
      J. Biol. Chem. 281:9852-9858(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, FUNCTION, INTERACTION WITH NSF, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCDK16_RAT
    AccessioniPrimary (citable) accession number: Q63686
    Secondary accession number(s): Q63687
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3