Q63686 (CDK16_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 16
EC=2.7.11.22

Alternative name(s):
Cell division protein kinase 16
PCTAIRE-motif protein kinase 1
Serine/threonine-protein kinase PCTAIRE-1

Gene names

Name:	Cdk16
Synonyms:	Pctaire1, Pctk1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Can phosphorylate CCNY at 'Ser-336' (in vitro) Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Regulates GH1 release by brain neurons. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development By similarity. Phosphorylates NSF, and thereby regulates NSF oligomerization. Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.3
Subunit structure	Interacts with BRSK2. Interacts with CCNY; this increases the CDK16 kinase activity By similarity. Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with NSF. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1. Ref.2 Ref.3
Subcellular location	Cytoplasm By similarity. Cytoplasmic vesicle › secretory vesicle. Cell junction › synapse › synaptosome. Note: Colocalizes with insulin in pancreas islets By similarity. Ref.3
Tissue specificity	Detected in brain (at protein level). Isoform 1 and isoform 2 are expressed in brain, kidney, lung, heart and lens fiber. Ref.1 Ref.3
Post-translational modification	Phosphorylation at Ser-153 inhibits kinase activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Cell junction Cytoplasm Cytoplasmic vesicle Synapse Synaptosome
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	exocytosis Inferred from sequence or structural similarity. Source: UniProtKB growth hormone secretion Inferred from sequence or structural similarity. Source: UniProtKB neuron projection development Inferred from sequence or structural similarity. Source: UniProtKB regulation of insulin secretion involved in cellular response to glucose stimulus Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to internal side of plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB neuron projection Inferred from electronic annotation. Source: UniProtKB-SubCell synaptic vesicle Inferred from direct assay Ref.3. Source: UniProtKB transport vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q63686-1)

Also known as: PCTAIRE-1a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q63686-2)

Also known as: PCTAIRE-1b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: MDRMKKIKRQ...ELRSIRGPLS → MYTNGYDEEIYYIGGKRVFLTPKAWPFPH

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Cyclin-dependent kinase 16		PRO_0000086486
Regions
	Domain	165 – 446	282	Protein kinase
	Nucleotide binding	171 – 179	9	ATP By similarity
Sites
	Active site	286	1	Proton acceptor By similarity
	Binding site	194	1	ATP By similarity
Amino acid modifications
	Modified residue	12	1	Phosphoserine; by BRSK2 By similarity
	Modified residue	78	1	Phosphoserine By similarity
	Modified residue	82	1	Phosphoserine By similarity
	Modified residue	95	1	Phosphoserine; by CDK5 By similarity
	Modified residue	110	1	Phosphoserine By similarity
	Modified residue	119	1	Phosphoserine By similarity
	Modified residue	138	1	Phosphoserine By similarity
	Modified residue	153	1	Phosphoserine By similarity
	Modified residue	155	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 64	64	MDRMK…RGPLS → MYTNGYDEEIYYIGGKRVFL TPKAWPFPH in isoform 2.		VSP_010639

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PCTAIRE-1a) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8E77DB7551A84329	FASTA	496	55,765
	10 20 30 40 50 60 MDRMKKIKRQ LSMTLRGGRG IDKTNGVPEQ IGLDESGGGG GMTLGEAPTR VAPGELRSIR 70 80 90 100 110 120 GPLSSAPEIV HEDMKMGSDG ESDQASATSS DEVQSPVRVR MRNHPPRKIS TEDINKCLSL 130 140 150 160 170 180 PADIRLPEGY LEKLTLNSPI GDKPLSRRLR PVSLSEIGFG KLETYIKLDK LGEGTYATVY 190 200 210 220 230 240 KGKSKLTDNL VALKEIRLEH EEGAPCTAIR EVSLLKDLKH ANIVTLHDII HTEKSLTLVF 250 260 270 280 290 300 EYLDKDLKQY LDDCGNVINM HNVKLFLFQL LRGLAYCHRQ KVLHRDLKPQ NLLINERGEL 310 320 330 340 350 360 KLADFGLAYA KSIPTKTYSN EVVTLWYRPP DILLGSTDYS GQIDMWGVGC IFYEMATGRP 370 380 390 400 410 420 LFPGSTVEEQ LHFIFRILGT PTEDTWPGIL SNEEFRTYNY PKYRAEALLR HAPRLECDGA 430 440 450 460 470 480 DLLTKLLQFE GRNRISAEDA MKHPFFLSLG ERIHKLPDTT SIFALKEVQL QKEANIRSTS 490 MPDSGRPAFR VVDTEF
	Isoform 2 (PCTAIRE-1b) [UniParc]. Checksum: F543D3C9615AF987	FASTA	461	52,523
	10 20 30 40 50 60 MYTNGYDEEI YYIGGKRVFL TPKAWPFPHS APEIVHEDMK MGSDGESDQA SATSSDEVQS 70 80 90 100 110 120 PVRVRMRNHP PRKISTEDIN KCLSLPADIR LPEGYLEKLT LNSPIGDKPL SRRLRPVSLS 130 140 150 160 170 180 EIGFGKLETY IKLDKLGEGT YATVYKGKSK LTDNLVALKE IRLEHEEGAP CTAIREVSLL 190 200 210 220 230 240 KDLKHANIVT LHDIIHTEKS LTLVFEYLDK DLKQYLDDCG NVINMHNVKL FLFQLLRGLA 250 260 270 280 290 300 YCHRQKVLHR DLKPQNLLIN ERGELKLADF GLAYAKSIPT KTYSNEVVTL WYRPPDILLG 310 320 330 340 350 360 STDYSGQIDM WGVGCIFYEM ATGRPLFPGS TVEEQLHFIF RILGTPTEDT WPGILSNEEF 370 380 390 400 410 420 RTYNYPKYRA EALLRHAPRL ECDGADLLTK LLQFEGRNRI SAEDAMKHPF FLSLGERIHK 430 440 450 460 LPDTTSIFAL KEVQLQKEAN IRSTSMPDSG RPAFRVVDTE F

References

[1]	"Expression of alternatively spliced PCTAIRE-1 mRNA in PC12 cells and neonatal rat brain." Gao C.Y., Chauthaiwale V.M., Rampalli A.M., Zelenka P.S. Gene 176:243-247(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Strain: New England Deaconess Hospital. Tissue: Adrenal medulla.
[2]	"Identification of tudor repeat associator with PCTAIRE 2 (Trap). A novel protein that interacts with the N-terminal domain of PCTAIRE 2 in rat brain." Hirose T., Kawabuchi M., Tamaru T., Okumura N., Nagai K., Okada M. Eur. J. Biochem. 267:2113-2121(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH CDK17 AND TDRD7.
[3]	"Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis." Liu Y., Cheng K., Gong K., Fu A.K., Ip N.Y. J. Biol. Chem. 281:9852-9858(2006) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, FUNCTION, INTERACTION WITH NSF, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36444 mRNA. Translation: AAC52912.1. U36444 mRNA. Translation: AAC52913.1.
IPI	IPI00211116. IPI00327483.
PIR	JC5110. JC5111.
RefSeq	NP_112339.1. NM_031077.1.
UniGene	Rn.16871.
3D structure databases
ProteinModelPortal	Q63686.
SMR	Q63686. Positions 173-446.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q63686. 1 interaction.
PTM databases
PhosphoSite	Q63686.
Proteomic databases
PaxDb	Q63686.
PRIDE	Q63686.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81741.
KEGG	rno:81741.
UCSC	RGD:620584. rat.
Organism-specific databases
CTD	5127.
RGD	620584. Cdk16.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
HOVERGEN	HBG014652.
InParanoid	Q63686.
KO	K08820.
Gene expression databases
ArrayExpress	Q63686.
Genevestigator	Q63686.
GermOnline	ENSRNOG00000008578. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615454.

Entry information

Entry name

CDK16_RAT

Accession

Primary (citable) accession number: Q63686
Secondary accession number(s): Q63687

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program