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Q63686

- CDK16_RAT

UniProt

Q63686 - CDK16_RAT

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Protein

Cyclin-dependent kinase 16

Gene
Cdk16, Pctaire1, Pctk1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Can phosphorylate CCNY at 'Ser-336' (in vitro) Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Regulates GH1 release by brain neurons. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development By similarity. Phosphorylates NSF, and thereby regulates NSF oligomerization.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei194 – 1941ATP By similarity
Active sitei286 – 2861Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi171 – 1799ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. exocytosis Source: UniProtKB
  2. growth hormone secretion Source: UniProtKB
  3. neuron projection development Source: UniProtKB
  4. regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  5. spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 16 (EC:2.7.11.22)
Alternative name(s):
Cell division protein kinase 16
PCTAIRE-motif protein kinase 1
Serine/threonine-protein kinase PCTAIRE-1
Gene namesi
Name:Cdk16
Synonyms:Pctaire1, Pctk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620584. Cdk16.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmic vesiclesecretory vesicle. Cell junctionsynapsesynaptosome
Note: Colocalizes with insulin in pancreas islets By similarity.1 Publication

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  3. neuron projection Source: UniProtKB-SubCell
  4. synaptic vesicle Source: UniProtKB
  5. transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Cyclin-dependent kinase 16PRO_0000086486Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphoserine; by BRSK2 By similarity
Modified residuei78 – 781Phosphoserine By similarity
Modified residuei82 – 821Phosphoserine By similarity
Modified residuei95 – 951Phosphoserine; by CDK5 By similarity
Modified residuei119 – 1191Phosphoserine By similarity
Modified residuei138 – 1381Phosphoserine By similarity
Modified residuei153 – 1531Phosphoserine By similarity
Modified residuei155 – 1551Phosphoserine By similarity

Post-translational modificationi

Phosphorylation at Ser-153 inhibits kinase activity By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63686.
PRIDEiQ63686.

PTM databases

PhosphoSiteiQ63686.

Expressioni

Tissue specificityi

Detected in brain (at protein level). Isoform 1 and isoform 2 are expressed in brain, kidney, lung, heart and lens fiber.2 Publications

Gene expression databases

GenevestigatoriQ63686.

Interactioni

Subunit structurei

Interacts with BRSK2. Interacts with CCNY; this increases the CDK16 kinase activity By similarity. Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with NSF. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1.2 Publications

Protein-protein interaction databases

IntActiQ63686. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ63686.
SMRiQ63686. Positions 173-446.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini165 – 446282Protein kinaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ63686.
KOiK08820.
PhylomeDBiQ63686.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q63686-1) [UniParc]FASTAAdd to Basket

Also known as: PCTAIRE-1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDRMKKIKRQ LSMTLRGGRG IDKTNGVPEQ IGLDESGGGG GMTLGEAPTR    50
VAPGELRSIR GPLSSAPEIV HEDMKMGSDG ESDQASATSS DEVQSPVRVR 100
MRNHPPRKIS TEDINKCLSL PADIRLPEGY LEKLTLNSPI GDKPLSRRLR 150
PVSLSEIGFG KLETYIKLDK LGEGTYATVY KGKSKLTDNL VALKEIRLEH 200
EEGAPCTAIR EVSLLKDLKH ANIVTLHDII HTEKSLTLVF EYLDKDLKQY 250
LDDCGNVINM HNVKLFLFQL LRGLAYCHRQ KVLHRDLKPQ NLLINERGEL 300
KLADFGLAYA KSIPTKTYSN EVVTLWYRPP DILLGSTDYS GQIDMWGVGC 350
IFYEMATGRP LFPGSTVEEQ LHFIFRILGT PTEDTWPGIL SNEEFRTYNY 400
PKYRAEALLR HAPRLECDGA DLLTKLLQFE GRNRISAEDA MKHPFFLSLG 450
ERIHKLPDTT SIFALKEVQL QKEANIRSTS MPDSGRPAFR VVDTEF 496
Length:496
Mass (Da):55,765
Last modified:November 1, 1996 - v1
Checksum:i8E77DB7551A84329
GO
Isoform 2 (identifier: Q63686-2) [UniParc]FASTAAdd to Basket

Also known as: PCTAIRE-1b

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: MDRMKKIKRQ...ELRSIRGPLS → MYTNGYDEEIYYIGGKRVFLTPKAWPFPH

Show »
Length:461
Mass (Da):52,523
Checksum:iF543D3C9615AF987
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6464MDRMK…RGPLS → MYTNGYDEEIYYIGGKRVFL TPKAWPFPH in isoform 2. VSP_010639Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36444 mRNA. Translation: AAC52912.1.
U36444 mRNA. Translation: AAC52913.1.
PIRiJC5110.
JC5111.
RefSeqiNP_112339.1. NM_031077.1. [Q63686-2]
UniGeneiRn.16871.

Genome annotation databases

GeneIDi81741.
KEGGirno:81741.
UCSCiRGD:620584. rat. [Q63686-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36444 mRNA. Translation: AAC52912.1 .
U36444 mRNA. Translation: AAC52913.1 .
PIRi JC5110.
JC5111.
RefSeqi NP_112339.1. NM_031077.1. [Q63686-2 ]
UniGenei Rn.16871.

3D structure databases

ProteinModelPortali Q63686.
SMRi Q63686. Positions 173-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q63686. 1 interaction.

PTM databases

PhosphoSitei Q63686.

Proteomic databases

PaxDbi Q63686.
PRIDEi Q63686.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 81741.
KEGGi rno:81741.
UCSCi RGD:620584. rat. [Q63686-1 ]

Organism-specific databases

CTDi 5127.
RGDi 620584. Cdk16.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
HOVERGENi HBG014652.
InParanoidi Q63686.
KOi K08820.
PhylomeDBi Q63686.

Miscellaneous databases

NextBioi 615454.
PROi Q63686.

Gene expression databases

Genevestigatori Q63686.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Expression of alternatively spliced PCTAIRE-1 mRNA in PC12 cells and neonatal rat brain."
    Gao C.Y., Chauthaiwale V.M., Rampalli A.M., Zelenka P.S.
    Gene 176:243-247(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: New England Deaconess Hospital.
    Tissue: Adrenal medulla.
  2. "Identification of tudor repeat associator with PCTAIRE 2 (Trap). A novel protein that interacts with the N-terminal domain of PCTAIRE 2 in rat brain."
    Hirose T., Kawabuchi M., Tamaru T., Okumura N., Nagai K., Okada M.
    Eur. J. Biochem. 267:2113-2121(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH CDK17 AND TDRD7.
  3. "Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis."
    Liu Y., Cheng K., Gong K., Fu A.K., Ip N.Y.
    J. Biol. Chem. 281:9852-9858(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION, INTERACTION WITH NSF, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCDK16_RAT
AccessioniPrimary (citable) accession number: Q63686
Secondary accession number(s): Q63687
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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