Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q63686 (CDK16_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 16

EC=2.7.11.22
Alternative name(s):
Cell division protein kinase 16
PCTAIRE-motif protein kinase 1
Serine/threonine-protein kinase PCTAIRE-1
Gene names
Name:Cdk16
Synonyms:Pctaire1, Pctk1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Can phosphorylate CCNY at 'Ser-336' (in vitro) Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Regulates GH1 release by brain neurons. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development By similarity. Phosphorylates NSF, and thereby regulates NSF oligomerization. Ref.3

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.3

Subunit structure

Interacts with BRSK2. Interacts with CCNY; this increases the CDK16 kinase activity By similarity. Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with NSF. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1. Ref.2 Ref.3

Subcellular location

Cytoplasm By similarity. Cytoplasmic vesiclesecretory vesicle. Cell junctionsynapsesynaptosome. Note: Colocalizes with insulin in pancreas islets By similarity. Ref.3

Tissue specificity

Detected in brain (at protein level). Isoform 1 and isoform 2 are expressed in brain, kidney, lung, heart and lens fiber. Ref.1 Ref.3

Post-translational modification

Phosphorylation at Ser-153 inhibits kinase activity By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processDifferentiation
Spermatogenesis
   Cellular componentCell junction
Cytoplasm
Cytoplasmic vesicle
Synapse
Synaptosome
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processexocytosis

Inferred from sequence or structural similarity. Source: UniProtKB

growth hormone secretion

Inferred from sequence or structural similarity. Source: UniProtKB

neuron projection development

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of insulin secretion involved in cellular response to glucose stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

spermatogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

extrinsic component of cytoplasmic side of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

neuron projection

Inferred from electronic annotation. Source: UniProtKB-SubCell

synaptic vesicle

Inferred from direct assay Ref.3. Source: UniProtKB

transport vesicle

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein serine/threonine kinase activity

Inferred from direct assay Ref.3. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q63686-1)

Also known as: PCTAIRE-1a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q63686-2)

Also known as: PCTAIRE-1b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: MDRMKKIKRQ...ELRSIRGPLS → MYTNGYDEEIYYIGGKRVFLTPKAWPFPH

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Cyclin-dependent kinase 16
PRO_0000086486

Regions

Domain165 – 446282Protein kinase
Nucleotide binding171 – 1799ATP By similarity

Sites

Active site2861Proton acceptor By similarity
Binding site1941ATP By similarity

Amino acid modifications

Modified residue121Phosphoserine; by BRSK2 By similarity
Modified residue781Phosphoserine By similarity
Modified residue821Phosphoserine By similarity
Modified residue951Phosphoserine; by CDK5 By similarity
Modified residue1191Phosphoserine By similarity
Modified residue1381Phosphoserine By similarity
Modified residue1531Phosphoserine By similarity
Modified residue1551Phosphoserine By similarity

Natural variations

Alternative sequence1 – 6464MDRMK…RGPLS → MYTNGYDEEIYYIGGKRVFL TPKAWPFPH in isoform 2.
VSP_010639

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PCTAIRE-1a) [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8E77DB7551A84329

FASTA49655,765
        10         20         30         40         50         60 
MDRMKKIKRQ LSMTLRGGRG IDKTNGVPEQ IGLDESGGGG GMTLGEAPTR VAPGELRSIR 

        70         80         90        100        110        120 
GPLSSAPEIV HEDMKMGSDG ESDQASATSS DEVQSPVRVR MRNHPPRKIS TEDINKCLSL 

       130        140        150        160        170        180 
PADIRLPEGY LEKLTLNSPI GDKPLSRRLR PVSLSEIGFG KLETYIKLDK LGEGTYATVY 

       190        200        210        220        230        240 
KGKSKLTDNL VALKEIRLEH EEGAPCTAIR EVSLLKDLKH ANIVTLHDII HTEKSLTLVF 

       250        260        270        280        290        300 
EYLDKDLKQY LDDCGNVINM HNVKLFLFQL LRGLAYCHRQ KVLHRDLKPQ NLLINERGEL 

       310        320        330        340        350        360 
KLADFGLAYA KSIPTKTYSN EVVTLWYRPP DILLGSTDYS GQIDMWGVGC IFYEMATGRP 

       370        380        390        400        410        420 
LFPGSTVEEQ LHFIFRILGT PTEDTWPGIL SNEEFRTYNY PKYRAEALLR HAPRLECDGA 

       430        440        450        460        470        480 
DLLTKLLQFE GRNRISAEDA MKHPFFLSLG ERIHKLPDTT SIFALKEVQL QKEANIRSTS 

       490 
MPDSGRPAFR VVDTEF 

« Hide

Isoform 2 (PCTAIRE-1b) [UniParc].

Checksum: F543D3C9615AF987
Show »

FASTA46152,523

References

[1]"Expression of alternatively spliced PCTAIRE-1 mRNA in PC12 cells and neonatal rat brain."
Gao C.Y., Chauthaiwale V.M., Rampalli A.M., Zelenka P.S.
Gene 176:243-247(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
Strain: New England Deaconess Hospital.
Tissue: Adrenal medulla.
[2]"Identification of tudor repeat associator with PCTAIRE 2 (Trap). A novel protein that interacts with the N-terminal domain of PCTAIRE 2 in rat brain."
Hirose T., Kawabuchi M., Tamaru T., Okumura N., Nagai K., Okada M.
Eur. J. Biochem. 267:2113-2121(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH CDK17 AND TDRD7.
[3]"Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis."
Liu Y., Cheng K., Gong K., Fu A.K., Ip N.Y.
J. Biol. Chem. 281:9852-9858(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, FUNCTION, INTERACTION WITH NSF, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U36444 mRNA. Translation: AAC52912.1.
U36444 mRNA. Translation: AAC52913.1.
PIRJC5110.
JC5111.
RefSeqNP_112339.1. NM_031077.1. [Q63686-2]
UniGeneRn.16871.

3D structure databases

ProteinModelPortalQ63686.
SMRQ63686. Positions 173-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ63686. 1 interaction.

PTM databases

PhosphoSiteQ63686.

Proteomic databases

PaxDbQ63686.
PRIDEQ63686.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID81741.
KEGGrno:81741.
UCSCRGD:620584. rat. [Q63686-1]

Organism-specific databases

CTD5127.
RGD620584. Cdk16.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
HOVERGENHBG014652.
InParanoidQ63686.
KOK08820.
PhylomeDBQ63686.

Gene expression databases

GenevestigatorQ63686.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio615454.
PROQ63686.

Entry information

Entry nameCDK16_RAT
AccessionPrimary (citable) accession number: Q63686
Secondary accession number(s): Q63687
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families