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Protein

Lysine-specific demethylase 3A

Gene

Kdm3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Directly regulates expression of PPARA and UCP1 and is involved in obesity resistance (By similarity).By similarity

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1006Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi1008Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi1135Iron; catalyticPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri546 – 571C6-typeSequence analysisAdd BLAST26

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • histone H3-K9 demethylation Source: GO_Central
  • male gonad development Source: RGD
  • positive regulation of gene expression Source: RGD
  • regulation of transcription, DNA-templated Source: GO_Central
  • response to hypoxia Source: RGD
  • spermatogenesis Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Chromatin regulator, Dioxygenase, Oxidoreductase
Biological processDifferentiation, Spermatogenesis, Transcription, Transcription regulation
LigandIron, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine-specific demethylase 3A (EC:1.14.11.-)
Alternative name(s):
JmjC domain-containing histone demethylation protein 2A
Jumonji domain-containing protein 1A
Testis-specific gene A protein
Zinc finger protein TSGA
Gene namesi
Name:Kdm3a
Synonyms:Jhdm2a, Jmjd1a, Tsga
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708351 Kdm3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000842871 – 1214Lysine-specific demethylase 3AAdd BLAST1214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei150PhosphoserineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Modified residuei330PhosphoserineBy similarity1
Modified residuei779N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ63679
PRIDEiQ63679

PTM databases

PhosphoSitePlusiQ63679

Expressioni

Tissue specificityi

Testis specific. Expressed only in male germ cells.

Developmental stagei

Reaches a maximum during the meiotic and the postmeiotic stages of germ cell development.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041218

Structurei

3D structure databases

ProteinModelPortaliQ63679
SMRiQ63679
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini944 – 1167JmjCPROSITE-ProRule annotationAdd BLAST224

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi769 – 773LXXLL motif5

Domaini

The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.By similarity
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.By similarity

Sequence similaritiesi

Belongs to the JHDM2 histone demethylase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri546 – 571C6-typeSequence analysisAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1356 Eukaryota
ENOG410XTAA LUCA
HOGENOMiHOG000007471
InParanoidiQ63679
KOiK15601

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
PfamiView protein in Pfam
PF02373 JmjC, 1 hit
SMARTiView protein in SMART
SM00558 JmjC, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit

Sequencei

Sequence statusi: Complete.

Q63679-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKSLLDKAG LGSITSVRFL GDQQSVFVSK DLLKPIQDVN SLRLSLTDNQ
60 70 80 90 100
TVSKEFQALI VKHLDESHLL QGDKNLVGSE VRIYSLDPST QWFSATVVHG
110 120 130 140 150
NPSSKTLQVN CEEIPALKIV DPALIHVEVV HDNFVTCGNS TRIGAVKRKS
160 170 180 190 200
SENNGSSVSK QAKSCSEVSP SMCPVQSVPT TVCKEILLGC TAATPSSNRQ
210 220 230 240 250
QNTPQAANSP PNIGAKLPQG CHKQSLPEEI SSCLNTKSEV LRTKPDVCKA
260 270 280 290 300
GLLSSKSSQV GAGDLKILSE PKGSCIQPKT NTDQESRLES TPQPVTGLTK
310 320 330 340 350
ECLVTKTSSK AELDNATAPE LQKRLEHTAS TPDGLSDKPE VEAGVTRLNS
360 370 380 390 400
CSEKKVGPSD LGSQSQNLKE TSVKVDHDSC CTRSSNKTQT PPARKSVLTD
410 420 430 440 450
PDKLKKLQQS GEAFVQDDSC VNIVAQLPKC RECRLDSLRK DKDQQKDSPV
460 470 480 490 500
FCRFFHFRRL QFNKHGVLRV EGFLTPNKYD SEAIGLWLPL TKNVVGTDLD
510 520 530 540 550
TAKYILANIG DHFCQMVISE KEAMSTIEPH RQVAWKRAVK GVREMCDVCD
560 570 580 590 600
TTIFNLHWVC PRCGFGVCVD CYRLKRKNCQ QGAAYKTFSW IRCVKSQIHE
610 620 630 640 650
PENLMPTQII PGKALYDVGD IVHSVRAKWG IKANCPCSNR QFKLFSKPAL
660 670 680 690 700
KEDLKQASLS GEKPSLGTMV QQSSPVLEPA AVCGEAPSKP ASNVKPICPA
710 720 730 740 750
NTSPLNWLAD LTSGNVNKEN KEKQLTMPIL KNEIKCLPPL PPLNKSSTVL
760 770 780 790 800
HTFNSTILTP VSNNNSGFLR NLLNSSTGKT ENGLKNTPKI LDDIFASLVQ
810 820 830 840 850
NKTSSDLSKR PQGLTIKPSI LGFDTPHYWL CDNRLLCLQD PNNKSNWNVF
860 870 880 890 900
RECWKQGQPV MVSGVHHKLN TELWKPESFR KEFGEQEVDL VNCRTNEIIT
910 920 930 940 950
GATVGDFWDG FEDVPNRLKN EKEKEPMVLK LKDWPPGEDF RDMMPSRFDD
960 970 980 990 1000
LMANIPLPEY TRRDGKLNLA SRLPNYFVRP DLGPKMYNAY GLITPEDRKY
1010 1020 1030 1040 1050
GTTNLHLDVS DAANVMVYVG IPKGQCEQEE EVLRTIQDGD SDELTIKRFI
1060 1070 1080 1090 1100
EGKEKPGALW HIYAAKDTEK IREFLKKVSE EQGQENPADH DPIHDQSWYL
1110 1120 1130 1140 1150
DRSLRKRLYQ EYGVQGWAIV QFLGDVVFIP AGAPHQVHNL YSCIKVAEDF
1160 1170 1180 1190 1200
VSPEHVKHCF WLTQEFRHLS QTHTNHEDKL QVKNVIYHAV KDAVAMLKAV
1210
NPVWANVNSS AHWR
Length:1,214
Mass (Da):135,404
Last modified:November 1, 1996 - v1
Checksum:i8E6B52220760EA6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59993 mRNA Translation: CAA42610.1
PIRiS28499
RefSeqiNP_786940.1, NM_175764.2
UniGeneiRn.10541

Genome annotation databases

GeneIDi312440
KEGGirno:312440
UCSCiRGD:708351 rat

Similar proteinsi

Entry informationi

Entry nameiKDM3A_RAT
AccessioniPrimary (citable) accession number: Q63679
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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