Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Upstream stimulatory factor 2

Gene

Usf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

GO - Molecular functioni

  • double-stranded DNA binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: RGD
  • sequence-specific DNA binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: RGD

GO - Biological processi

  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Upstream stimulatory factor 2
Alternative name(s):
Major late transcription factor 2
Upstream transcription factor 2
Gene namesi
Name:Usf2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620975. Usf2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Upstream stimulatory factor 2PRO_0000127502Add
BLAST

Proteomic databases

PaxDbiQ63665.

PTM databases

iPTMnetiQ63665.
PhosphoSiteiQ63665.

Expressioni

Gene expression databases

BgeeiENSRNOG00000021030.

Interactioni

Subunit structurei

Interacts with MAF (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2).By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028550.

Structurei

3D structure databases

ProteinModelPortaliQ63665.
SMRiQ63665. Positions 233-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini235 – 29056bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni307 – 32822Leucine-zipperAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410INSC. Eukaryota.
ENOG4111DV1. LUCA.
HOGENOMiHOG000294174.
HOVERGENiHBG004346.
InParanoidiQ63665.
KOiK09106.
PhylomeDBiQ63665.
TreeFamiTF323338.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Note: At least 2 isoforms are produced.
Isoform 1 (identifier: Q63665-1) [UniParc]FASTAAdd to basket
Also known as: USF2L, USF2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA
60 70 80 90 100
IASVQQAAFG DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS
110 120 130 140 150
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF
160 170 180 190 200
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM
210 220 230 240 250
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK
260 270 280 290 300
INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET
310 320 330 340
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ
Length:346
Mass (Da):36,954
Last modified:December 6, 2005 - v2
Checksum:iE9D216BC25F9447B
GO
Isoform 2 (identifier: Q63665-2) [UniParc]FASTAAdd to basket
Also known as: USF2delta1

The sequence of this isoform differs from the canonical sequence as follows:
     117-143: Missing.

Show »
Length:319
Mass (Da):34,500
Checksum:i5A5D8BC8007BB90F
GO
Isoform 3 (identifier: Q63665-3) [UniParc]FASTAAdd to basket
Also known as: USF2delta2, USF2B

The sequence of this isoform differs from the canonical sequence as follows:
     77-143: Missing.

Show »
Length:279
Mass (Da):30,595
Checksum:iD9474CE884D8A60B
GO
Isoform 4 (identifier: Q63665-4) [UniParc]FASTAAdd to basket
Also known as: USF2delta3

The sequence of this isoform differs from the canonical sequence as follows:
     21-27: SHDKGPE → RRGRPGG
     28-346: Missing.

Show »
Length:27
Mass (Da):2,613
Checksum:iB5C9C83F09C71D5A
GO
Isoform 5 (identifier: Q63665-5) [UniParc]FASTAAdd to basket
Also known as: USF2delta4

The sequence of this isoform differs from the canonical sequence as follows:
     21-22: SH → RL
     23-346: Missing.

Show »
Length:22
Mass (Da):2,145
Checksum:i243D5A861D66A6DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951A → T in CAA62338 (PubMed:8576131).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei21 – 277SHDKGPE → RRGRPGG in isoform 4. 1 PublicationVSP_016542
Alternative sequencei21 – 222SH → RL in isoform 5. 1 PublicationVSP_016543
Alternative sequencei23 – 346324Missing in isoform 5. 1 PublicationVSP_016544Add
BLAST
Alternative sequencei28 – 346319Missing in isoform 4. 1 PublicationVSP_016545Add
BLAST
Alternative sequencei77 – 14367Missing in isoform 3. 1 PublicationVSP_016546Add
BLAST
Alternative sequencei117 – 14327Missing in isoform 2. 1 PublicationVSP_016547Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035647 mRNA. Translation: BAB19964.1.
AB035648 mRNA. Translation: BAB19965.1.
AB035649 mRNA. Translation: BAB19966.1.
AB035650 mRNA. Translation: BAB19967.1.
AB035651 mRNA. Translation: BAB19968.1.
AB047556 mRNA. Translation: BAB20993.1.
X90823 mRNA. Translation: CAA62338.1.
RefSeqiNP_112401.1. NM_031139.1. [Q63665-1]
UniGeneiRn.44637.

Genome annotation databases

GeneIDi81817.
KEGGirno:81817.
UCSCiRGD:620975. rat. [Q63665-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035647 mRNA. Translation: BAB19964.1.
AB035648 mRNA. Translation: BAB19965.1.
AB035649 mRNA. Translation: BAB19966.1.
AB035650 mRNA. Translation: BAB19967.1.
AB035651 mRNA. Translation: BAB19968.1.
AB047556 mRNA. Translation: BAB20993.1.
X90823 mRNA. Translation: CAA62338.1.
RefSeqiNP_112401.1. NM_031139.1. [Q63665-1]
UniGeneiRn.44637.

3D structure databases

ProteinModelPortaliQ63665.
SMRiQ63665. Positions 233-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028550.

PTM databases

iPTMnetiQ63665.
PhosphoSiteiQ63665.

Proteomic databases

PaxDbiQ63665.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81817.
KEGGirno:81817.
UCSCiRGD:620975. rat. [Q63665-1]

Organism-specific databases

CTDi7392.
RGDi620975. Usf2.

Phylogenomic databases

eggNOGiENOG410INSC. Eukaryota.
ENOG4111DV1. LUCA.
HOGENOMiHOG000294174.
HOVERGENiHBG004346.
InParanoidiQ63665.
KOiK09106.
PhylomeDBiQ63665.
TreeFamiTF323338.

Miscellaneous databases

PROiQ63665.

Gene expression databases

BgeeiENSRNOG00000021030.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUSF2_RAT
AccessioniPrimary (citable) accession number: Q63665
Secondary accession number(s): Q76MJ1
, Q76MU2, Q9EQX1, Q9EQX2, Q9EQX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.