Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-sensitive inward rectifier potassium channel 8

Gene

Kcnj8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei170Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • defense response to virus Source: RGD
  • heart development Source: RGD
  • kidney development Source: RGD
  • membrane repolarization during ventricular cardiac muscle cell action potential Source: RGD
  • potassium ion import Source: RGD
  • potassium ion import across plasma membrane Source: RGD
  • potassium ion transport Source: RGD
  • response to exogenous dsRNA Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to pH Source: RGD
  • vasodilation Source: RGD

Keywordsi

Molecular functionIon channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-RNO-1296025. ATP sensitive Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 8
Alternative name(s):
Inward rectifier K(+) channel Kir6.1
Potassium channel, inwardly rectifying subfamily J member 8
uKATP-1
Gene namesi
Name:Kcnj8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2960. Kcnj8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 69CytoplasmicBy similarityAdd BLAST69
Transmembranei70 – 94Helical; Name=M1By similarityAdd BLAST25
Topological domaini95 – 126ExtracellularBy similarityAdd BLAST32
Intramembranei127 – 138Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei139 – 145Pore-formingBy similarity7
Topological domaini146 – 154ExtracellularBy similarity9
Transmembranei155 – 176Helical; Name=M2By similarityAdd BLAST22
Topological domaini177 – 424CytoplasmicBy similarityAdd BLAST248

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001549491 – 424ATP-sensitive inward rectifier potassium channel 8Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63664.

PTM databases

iPTMnetiQ63664.
PhosphoSitePlusiQ63664.

Expressioni

Tissue specificityi

Widely expressed, including in pancreatic islets, pituitary, skeletal muscle and heart.1 Publication

Gene expression databases

BgeeiENSRNOG00000013463.
GenevisibleiQ63664. RN.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • sulfonylurea receptor binding Source: RGD

Protein-protein interaction databases

CORUMiQ63664.
IntActiQ63664. 3 interactors.
MINTiMINT-8297391.
STRINGi10116.ENSRNOP00000018057.

Structurei

3D structure databases

ProteinModelPortaliQ63664.
SMRiQ63664.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi140 – 145Selectivity filterBy similarity6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiQ63664.
KOiK05001.
OMAiAMELQCS.
OrthoDBiEOG091G08HC.
PhylomeDBiQ63664.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiView protein in InterPro
IPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003278. K_chnl_inward-rec_Kir6.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF11. PTHR11767:SF11. 1 hit.
PfamiView protein in Pfam
PF01007. IRK. 1 hit.
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01331. KIR61CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLARKSIIPE EYVLARIAAE NLRKPRIRDR LPKARFIAKS GACNLAHKNI
60 70 80 90 100
REQGRFLQDI FTTLVDLKWR HTLVIFTMSF LCSWLLFAIM WWLVAFAHGD
110 120 130 140 150
IYAYMEKGIT EKSGLESAVC VTNVRSFTSA FLFSIEVQVT IGFGGRMMTE
160 170 180 190 200
ECPLAITVLI LQNIVGLIIN AVMLGCIFMK TAQAHRRAET LIFSRHAVIA
210 220 230 240 250
VRNGKLCFMF RVGDLRKSMI ISASVRIQVV KKTTTPEGEV VPIHQQDIPV
260 270 280 290 300
DNPIESNNIF LVAPLIICHV IDKRSPLYDI SATDLVNQDL EVIVILEGVV
310 320 330 340 350
ETTGITTQAR TSYIAEEIQW GHRFVSIVTE EEGVYSVDYS KFGNTVRVAA
360 370 380 390 400
PRCSARELDE KPSILIQTLQ KSELSHQNSL RKRNSMRRNN SMRRSNSIRR
410 420
NNSSLMVPKV QFMTPEGNQC PSES
Length:424
Mass (Da):47,963
Last modified:November 1, 1996 - v1
Checksum:i22548313B92FE095
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti23R → H. 1
Natural varianti48K → Q. 1
Natural varianti150E → G. 1
Natural varianti170N → D. 1
Natural varianti241V → A. 1
Natural varianti379S → F. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42145 mRNA. Translation: BAA07716.1.
AB043636 mRNA. Translation: BAA96237.1.
AB043637 mRNA. Translation: BAA96238.1.
PIRiI60373.
RefSeqiNP_058795.3. NM_017099.4.
UniGeneiRn.229203.

Genome annotation databases

EnsembliENSRNOT00000018057; ENSRNOP00000018057; ENSRNOG00000013463.
GeneIDi25472.
KEGGirno:25472.
UCSCiRGD:2960. rat.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKCNJ8_RAT
AccessioniPrimary (citable) accession number: Q63664
Secondary accession number(s): Q9JM49, Q9JM50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families