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Q63664

- KCNJ8_RAT

UniProt

Q63664 - KCNJ8_RAT

Protein

ATP-sensitive inward rectifier potassium channel 8

Gene

Kcnj8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei170 – 1701Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. ATP-activated inward rectifier potassium channel activity Source: RGD
    2. ATP binding Source: RGD
    3. protein binding Source: RGD
    4. sulfonylurea receptor binding Source: RGD

    GO - Biological processi

    1. defense response to virus Source: Ensembl
    2. heart development Source: Ensembl
    3. kidney development Source: RGD
    4. potassium ion import Source: RGD
    5. potassium ion transport Source: RGD
    6. response to exogenous dsRNA Source: Ensembl
    7. response to lipopolysaccharide Source: Ensembl
    8. response to pH Source: RGD
    9. vasodilation Source: RGD

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199215. ATP sensitive Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-sensitive inward rectifier potassium channel 8
    Alternative name(s):
    Inward rectifier K(+) channel Kir6.1
    Potassium channel, inwardly rectifying subfamily J member 8
    uKATP-1
    Gene namesi
    Name:Kcnj8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi2960. Kcnj8.

    Subcellular locationi

    GO - Cellular componenti

    1. ATP-sensitive potassium channel complex Source: RGD
    2. intracellular membrane-bounded organelle Source: RGD
    3. membrane Source: RGD
    4. mitochondrion Source: RGD
    5. myofibril Source: Ensembl
    6. sarcolemma Source: RGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 424424ATP-sensitive inward rectifier potassium channel 8PRO_0000154949Add
    BLAST

    Proteomic databases

    PaxDbiQ63664.

    PTM databases

    PhosphoSiteiQ63664.

    Expressioni

    Tissue specificityi

    Expressed ubiquitously, including pancreatic islets, pituitary, skeletal muscle and heart.

    Gene expression databases

    GenevestigatoriQ63664.

    Interactioni

    Protein-protein interaction databases

    IntActiQ63664. 1 interaction.
    MINTiMINT-8297391.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63664.
    SMRiQ63664. Positions 184-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6969CytoplasmicBy similarityAdd
    BLAST
    Topological domaini95 – 12632ExtracellularBy similarityAdd
    BLAST
    Topological domaini146 – 1549ExtracellularBy similarity
    Topological domaini177 – 424248CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei127 – 13812Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei139 – 1457Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei70 – 9425Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei155 – 17622Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi140 – 1456Selectivity filterBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    GeneTreeiENSGT00690000102074.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiQ63664.
    KOiK05001.
    OMAiVIAVRNN.
    OrthoDBiEOG7XPZ5K.
    PhylomeDBiQ63664.
    TreeFamiTF313676.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003278. K_chnl_inward-rec_Kir6.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01331. KIR61CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q63664-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLARKSIIPE EYVLARIAAE NLRKPRIRDR LPKARFIAKS GACNLAHKNI    50
    REQGRFLQDI FTTLVDLKWR HTLVIFTMSF LCSWLLFAIM WWLVAFAHGD 100
    IYAYMEKGIT EKSGLESAVC VTNVRSFTSA FLFSIEVQVT IGFGGRMMTE 150
    ECPLAITVLI LQNIVGLIIN AVMLGCIFMK TAQAHRRAET LIFSRHAVIA 200
    VRNGKLCFMF RVGDLRKSMI ISASVRIQVV KKTTTPEGEV VPIHQQDIPV 250
    DNPIESNNIF LVAPLIICHV IDKRSPLYDI SATDLVNQDL EVIVILEGVV 300
    ETTGITTQAR TSYIAEEIQW GHRFVSIVTE EEGVYSVDYS KFGNTVRVAA 350
    PRCSARELDE KPSILIQTLQ KSELSHQNSL RKRNSMRRNN SMRRSNSIRR 400
    NNSSLMVPKV QFMTPEGNQC PSES 424
    Length:424
    Mass (Da):47,963
    Last modified:November 1, 1996 - v1
    Checksum:i22548313B92FE095
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti23 – 231R → H.
    Natural varianti48 – 481K → Q.
    Natural varianti150 – 1501E → G.
    Natural varianti170 – 1701N → D.
    Natural varianti241 – 2411V → A.
    Natural varianti379 – 3791S → F.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42145 mRNA. Translation: BAA07716.1.
    AB043636 mRNA. Translation: BAA96237.1.
    AB043637 mRNA. Translation: BAA96238.1.
    PIRiI60373.
    RefSeqiNP_058795.3. NM_017099.4.
    UniGeneiRn.229203.

    Genome annotation databases

    EnsembliENSRNOT00000018057; ENSRNOP00000018057; ENSRNOG00000013463.
    GeneIDi25472.
    KEGGirno:25472.
    UCSCiRGD:2960. rat.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42145 mRNA. Translation: BAA07716.1 .
    AB043636 mRNA. Translation: BAA96237.1 .
    AB043637 mRNA. Translation: BAA96238.1 .
    PIRi I60373.
    RefSeqi NP_058795.3. NM_017099.4.
    UniGenei Rn.229203.

    3D structure databases

    ProteinModelPortali Q63664.
    SMRi Q63664. Positions 184-361.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q63664. 1 interaction.
    MINTi MINT-8297391.

    Chemistry

    BindingDBi Q63664.

    PTM databases

    PhosphoSitei Q63664.

    Proteomic databases

    PaxDbi Q63664.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000018057 ; ENSRNOP00000018057 ; ENSRNOG00000013463 .
    GeneIDi 25472.
    KEGGi rno:25472.
    UCSCi RGD:2960. rat.

    Organism-specific databases

    CTDi 3764.
    RGDi 2960. Kcnj8.

    Phylogenomic databases

    eggNOGi NOG72812.
    GeneTreei ENSGT00690000102074.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi Q63664.
    KOi K05001.
    OMAi VIAVRNN.
    OrthoDBi EOG7XPZ5K.
    PhylomeDBi Q63664.
    TreeFami TF313676.

    Enzyme and pathway databases

    Reactomei REACT_199215. ATP sensitive Potassium channels.

    Miscellaneous databases

    NextBioi 606777.
    PROi Q63664.

    Gene expression databases

    Genevestigatori Q63664.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003278. K_chnl_inward-rec_Kir6.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01331. KIR61CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional characterization of a novel ATP-sensitive potassium channel ubiquitously expressed in rat tissues, including pancreatic islets, pituitary, skeletal muscle, and heart."
      Inagaki N., Tsuura Y., Namba N., Masuda K., Gonoi T., Horie M., Seino Y., Mizuta M., Seino S.
      J. Biol. Chem. 270:5691-5694(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Pancreatic islet.
    2. Cao K., Wang R.
      Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
      Strain: Sprague-Dawley.
      Tissue: Vascular smooth muscle.

    Entry informationi

    Entry nameiKCNJ8_RAT
    AccessioniPrimary (citable) accession number: Q63664
    Secondary accession number(s): Q9JM49, Q9JM50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3