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Q63664 (IRK8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-sensitive inward rectifier potassium channel 8
Alternative name(s):
Inward rectifier K(+) channel Kir6.1
Potassium channel, inwardly rectifying subfamily J member 8
uKATP-1
Gene names
Name:Kcnj8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed ubiquitously, including pancreatic islets, pituitary, skeletal muscle and heart.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ8 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   LigandPotassium
   Molecular functionIon channel
Voltage-gated channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to virus

Inferred from electronic annotation. Source: Ensembl

heart development

Inferred from electronic annotation. Source: Ensembl

kidney development

Inferred from expression pattern PubMed 12466933. Source: RGD

potassium ion import

Inferred from direct assay Ref.1. Source: RGD

potassium ion transport

Inferred from direct assay PubMed 17097686. Source: RGD

response to exogenous dsRNA

Inferred from electronic annotation. Source: Ensembl

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to pH

Inferred from direct assay PubMed 12677015. Source: RGD

vasodilation

Inferred from direct assay PubMed 12677015. Source: RGD

   Cellular_componentATP-sensitive potassium channel complex

Inferred from direct assay PubMed 17097686. Source: RGD

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 15983113. Source: RGD

membrane

Inferred from direct assay PubMed 15499025. Source: RGD

mitochondrion

Inferred from direct assay PubMed 15983113PubMed 16050978. Source: RGD

myofibril

Inferred from electronic annotation. Source: Ensembl

sarcolemma

Inferred from direct assay PubMed 15647111. Source: RGD

   Molecular_functionATP binding

Inferred from direct assay PubMed 15775962. Source: RGD

ATP-activated inward rectifier potassium channel activity

Inferred from direct assay PubMed 17097686Ref.1. Source: RGD

sulfonylurea receptor binding

Inferred from physical interaction PubMed 20123112. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 424424ATP-sensitive inward rectifier potassium channel 8
PRO_0000154949

Regions

Topological domain1 – 6969Cytoplasmic By similarity
Transmembrane70 – 9425Helical; Name=M1; By similarity
Topological domain95 – 12632Extracellular By similarity
Intramembrane127 – 13812Helical; Pore-forming; Name=H5; By similarity
Intramembrane139 – 1457Pore-forming; By similarity
Topological domain146 – 1549Extracellular By similarity
Transmembrane155 – 17622Helical; Name=M2; By similarity
Topological domain177 – 424248Cytoplasmic By similarity
Motif140 – 1456Selectivity filter By similarity

Sites

Site1701Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Natural variations

Natural variant231R → H.
Natural variant481K → Q.
Natural variant1501E → G.
Natural variant1701N → D.
Natural variant2411V → A.
Natural variant3791S → F.

Sequences

Sequence LengthMass (Da)Tools
Q63664 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 22548313B92FE095

FASTA42447,963
        10         20         30         40         50         60 
MLARKSIIPE EYVLARIAAE NLRKPRIRDR LPKARFIAKS GACNLAHKNI REQGRFLQDI 

        70         80         90        100        110        120 
FTTLVDLKWR HTLVIFTMSF LCSWLLFAIM WWLVAFAHGD IYAYMEKGIT EKSGLESAVC 

       130        140        150        160        170        180 
VTNVRSFTSA FLFSIEVQVT IGFGGRMMTE ECPLAITVLI LQNIVGLIIN AVMLGCIFMK 

       190        200        210        220        230        240 
TAQAHRRAET LIFSRHAVIA VRNGKLCFMF RVGDLRKSMI ISASVRIQVV KKTTTPEGEV 

       250        260        270        280        290        300 
VPIHQQDIPV DNPIESNNIF LVAPLIICHV IDKRSPLYDI SATDLVNQDL EVIVILEGVV 

       310        320        330        340        350        360 
ETTGITTQAR TSYIAEEIQW GHRFVSIVTE EEGVYSVDYS KFGNTVRVAA PRCSARELDE 

       370        380        390        400        410        420 
KPSILIQTLQ KSELSHQNSL RKRNSMRRNN SMRRSNSIRR NNSSLMVPKV QFMTPEGNQC 


PSES 

« Hide

References

[1]"Cloning and functional characterization of a novel ATP-sensitive potassium channel ubiquitously expressed in rat tissues, including pancreatic islets, pituitary, skeletal muscle, and heart."
Inagaki N., Tsuura Y., Namba N., Masuda K., Gonoi T., Horie M., Seino Y., Mizuta M., Seino S.
J. Biol. Chem. 270:5691-5694(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Pancreatic islet.
[2]Cao K., Wang R.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
Strain: Sprague-Dawley.
Tissue: Vascular smooth muscle.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D42145 mRNA. Translation: BAA07716.1.
AB043636 mRNA. Translation: BAA96237.1.
AB043637 mRNA. Translation: BAA96238.1.
PIRI60373.
RefSeqNP_058795.3. NM_017099.4.
UniGeneRn.229203.

3D structure databases

ProteinModelPortalQ63664.
SMRQ63664. Positions 184-361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ63664. 1 interaction.
MINTMINT-8297391.

Chemistry

BindingDBQ63664.

PTM databases

PhosphoSiteQ63664.

Proteomic databases

PaxDbQ63664.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000018057; ENSRNOP00000018057; ENSRNOG00000013463.
GeneID25472.
KEGGrno:25472.
UCSCRGD:2960. rat.

Organism-specific databases

CTD3764.
RGD2960. Kcnj8.

Phylogenomic databases

eggNOGNOG72812.
GeneTreeENSGT00690000102074.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidQ63664.
KOK05001.
OMAVIAVRNN.
OrthoDBEOG7XPZ5K.
PhylomeDBQ63664.
TreeFamTF313676.

Gene expression databases

GenevestigatorQ63664.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003278. K_chnl_inward-rec_Kir6.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01331. KIR61CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio606777.
PROQ63664.

Entry information

Entry nameIRK8_RAT
AccessionPrimary (citable) accession number: Q63664
Secondary accession number(s): Q9JM49, Q9JM50
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families