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Protein

ATP-sensitive inward rectifier potassium channel 8

Gene

Kcnj8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei170 – 1701Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

  1. ATP-activated inward rectifier potassium channel activity Source: RGD
  2. ATP binding Source: RGD
  3. sulfonylurea receptor binding Source: RGD

GO - Biological processi

  1. defense response to virus Source: Ensembl
  2. heart development Source: Ensembl
  3. kidney development Source: RGD
  4. potassium ion import Source: RGD
  5. potassium ion transport Source: RGD
  6. regulation of ion transmembrane transport Source: GO_Central
  7. response to exogenous dsRNA Source: Ensembl
  8. response to lipopolysaccharide Source: Ensembl
  9. response to pH Source: RGD
  10. vasodilation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_302029. ATP sensitive Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 8
Alternative name(s):
Inward rectifier K(+) channel Kir6.1
Potassium channel, inwardly rectifying subfamily J member 8
uKATP-1
Gene namesi
Name:Kcnj8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2960. Kcnj8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6969CytoplasmicBy similarityAdd
BLAST
Transmembranei70 – 9425Helical; Name=M1By similarityAdd
BLAST
Topological domaini95 – 12632ExtracellularBy similarityAdd
BLAST
Intramembranei127 – 13812Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei139 – 1457Pore-formingBy similarity
Topological domaini146 – 1549ExtracellularBy similarity
Transmembranei155 – 17622Helical; Name=M2By similarityAdd
BLAST
Topological domaini177 – 424248CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. ATP-sensitive potassium channel complex Source: RGD
  2. intracellular membrane-bounded organelle Source: RGD
  3. membrane Source: RGD
  4. mitochondrion Source: RGD
  5. myofibril Source: Ensembl
  6. sarcolemma Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424ATP-sensitive inward rectifier potassium channel 8PRO_0000154949Add
BLAST

Proteomic databases

PaxDbiQ63664.

PTM databases

PhosphoSiteiQ63664.

Expressioni

Tissue specificityi

Expressed ubiquitously, including pancreatic islets, pituitary, skeletal muscle and heart.

Gene expression databases

GenevestigatoriQ63664.

Interactioni

Protein-protein interaction databases

IntActiQ63664. 1 interaction.
MINTiMINT-8297391.

Structurei

3D structure databases

ProteinModelPortaliQ63664.
SMRiQ63664. Positions 184-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi140 – 1456Selectivity filterBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiQ63664.
KOiK05001.
OMAiYAYMEKG.
OrthoDBiEOG7XPZ5K.
PhylomeDBiQ63664.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003278. K_chnl_inward-rec_Kir6.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01331. KIR61CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLARKSIIPE EYVLARIAAE NLRKPRIRDR LPKARFIAKS GACNLAHKNI
60 70 80 90 100
REQGRFLQDI FTTLVDLKWR HTLVIFTMSF LCSWLLFAIM WWLVAFAHGD
110 120 130 140 150
IYAYMEKGIT EKSGLESAVC VTNVRSFTSA FLFSIEVQVT IGFGGRMMTE
160 170 180 190 200
ECPLAITVLI LQNIVGLIIN AVMLGCIFMK TAQAHRRAET LIFSRHAVIA
210 220 230 240 250
VRNGKLCFMF RVGDLRKSMI ISASVRIQVV KKTTTPEGEV VPIHQQDIPV
260 270 280 290 300
DNPIESNNIF LVAPLIICHV IDKRSPLYDI SATDLVNQDL EVIVILEGVV
310 320 330 340 350
ETTGITTQAR TSYIAEEIQW GHRFVSIVTE EEGVYSVDYS KFGNTVRVAA
360 370 380 390 400
PRCSARELDE KPSILIQTLQ KSELSHQNSL RKRNSMRRNN SMRRSNSIRR
410 420
NNSSLMVPKV QFMTPEGNQC PSES
Length:424
Mass (Da):47,963
Last modified:November 1, 1996 - v1
Checksum:i22548313B92FE095
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti23 – 231R → H.
Natural varianti48 – 481K → Q.
Natural varianti150 – 1501E → G.
Natural varianti170 – 1701N → D.
Natural varianti241 – 2411V → A.
Natural varianti379 – 3791S → F.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42145 mRNA. Translation: BAA07716.1.
AB043636 mRNA. Translation: BAA96237.1.
AB043637 mRNA. Translation: BAA96238.1.
PIRiI60373.
RefSeqiNP_058795.3. NM_017099.4.
UniGeneiRn.229203.

Genome annotation databases

EnsembliENSRNOT00000018057; ENSRNOP00000018057; ENSRNOG00000013463.
GeneIDi25472.
KEGGirno:25472.
UCSCiRGD:2960. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42145 mRNA. Translation: BAA07716.1.
AB043636 mRNA. Translation: BAA96237.1.
AB043637 mRNA. Translation: BAA96238.1.
PIRiI60373.
RefSeqiNP_058795.3. NM_017099.4.
UniGeneiRn.229203.

3D structure databases

ProteinModelPortaliQ63664.
SMRiQ63664. Positions 184-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63664. 1 interaction.
MINTiMINT-8297391.

PTM databases

PhosphoSiteiQ63664.

Proteomic databases

PaxDbiQ63664.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018057; ENSRNOP00000018057; ENSRNOG00000013463.
GeneIDi25472.
KEGGirno:25472.
UCSCiRGD:2960. rat.

Organism-specific databases

CTDi3764.
RGDi2960. Kcnj8.

Phylogenomic databases

eggNOGiNOG72812.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiQ63664.
KOiK05001.
OMAiYAYMEKG.
OrthoDBiEOG7XPZ5K.
PhylomeDBiQ63664.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiREACT_302029. ATP sensitive Potassium channels.

Miscellaneous databases

NextBioi606777.
PROiQ63664.

Gene expression databases

GenevestigatoriQ63664.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003278. K_chnl_inward-rec_Kir6.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01331. KIR61CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and functional characterization of a novel ATP-sensitive potassium channel ubiquitously expressed in rat tissues, including pancreatic islets, pituitary, skeletal muscle, and heart."
    Inagaki N., Tsuura Y., Namba N., Masuda K., Gonoi T., Horie M., Seino Y., Mizuta M., Seino S.
    J. Biol. Chem. 270:5691-5694(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Pancreatic islet.
  2. Cao K., Wang R.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
    Strain: Sprague-Dawley.
    Tissue: Vascular smooth muscle.

Entry informationi

Entry nameiKCNJ8_RAT
AccessioniPrimary (citable) accession number: Q63664
Secondary accession number(s): Q9JM49, Q9JM50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.