Q63652 (OPSB_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Short-wave-sensitive opsin 1
Short name=S opsin

Alternative name(s):
Blue cone photoreceptor pigment
Blue-sensitive opsin
Short name=BOP
Short wavelength-sensitive cone opsin

Gene names

Name:	Opn1sw
Synonyms:	Bcp

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expresssed in cone photoreceptor cells.
Post-translational modification	Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Short-wave-sensitive opsin 1		PRO_0000197767
Regions
	Topological domain	1 – 31	31	Extracellular
	Transmembrane	32 – 56	25	Helical; Name=1; Potential
	Topological domain	57 – 68	12	Cytoplasmic
	Transmembrane	69 – 94	26	Helical; Name=2; Potential
	Topological domain	95 – 108	14	Extracellular
	Transmembrane	109 – 128	20	Helical; Name=3; Potential
	Topological domain	129 – 147	19	Cytoplasmic
	Transmembrane	148 – 171	24	Helical; Name=4; Potential
	Topological domain	172 – 197	26	Extracellular
	Transmembrane	198 – 225	28	Helical; Name=5; Potential
	Topological domain	226 – 247	22	Cytoplasmic
	Transmembrane	248 – 271	24	Helical; Name=6; Potential
	Topological domain	272 – 279	8	Extracellular
	Transmembrane	280 – 304	25	Helical; Name=7; Potential
	Topological domain	305 – 346	42	Cytoplasmic
Amino acid modifications
	Modified residue	291	1	N6-(retinylidene)lysine
	Glycosylation	12	1	N-linked (GlcNAc...) Probable
	Disulfide bond	105 ↔ 182		Potential
Experimental info
	Sequence conflict	70	1	I → V in AAC05294. Ref.2
	Sequence conflict	173	1	F → L in AAC05294. Ref.2
	Sequence conflict	279	1	Y → D in AAC05294. Ref.2
	Sequence conflict	329	1	G → S in AAC05294. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q63652 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: 6DE181761AD4F296	FASTA	346	39,062
	10 20 30 40 50 60 MSGEDEFYLF QNISSVGPWD GPQYHIAPVW AFHLQAAFMG FVFFAGTPLN ATVLVATLHY 70 80 90 100 110 120 KKLRQPLNYI LVNVSLGGFL FCIFSVFTVF IASCHGYFLF GRHVCALEAF LGSVAGLVTG 130 140 150 160 170 180 WSLAFLAFER YLVICKPFGN IRFNSKHALT VVLITWTIGI GVSIPPFFGW SRFIPEGLQC 190 200 210 220 230 240 SCGPDWYTVG TKYRSEHYTW FLFIFCFIIP LSLICFSYFQ LLRTLRAVAA QQQESATTQK 250 260 270 280 290 300 AEREVSHMVV VMVGSFCLCY VPYAALAMYM VNNRNHGLYL RLVTIPAFFS KSSCVYNPII 310 320 330 340 YCFMNKQFRA CILEMVCRKP MTDESDMSGS QKTEVSTVSS SKVGPH

References

[1]	"Molecular cloning and localization of rhodopsin kinase in the mammalian pineal." Zhao X., Haeseleer F., Fariss R.N., Huang J., Baehr W., Milam A.H., Palczewski K. Vis. Neurosci. 14:225-232(1997) [PubMed: 9147475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Pineal gland.
[2]	Delahaye-Brown A.-M. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63972 mRNA. Translation: AAB05931.1. AF051163 mRNA. Translation: AAC05294.1.
IPI	IPI00211060.
RefSeq	NP_112277.1. NM_031015.1.
UniGene	Rn.10549.
3D structure databases
ProteinModelPortal	Q63652.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63652.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q63652.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81644.
KEGG	rno:81644.
UCSC	NM_031015. rat.
Organism-specific databases
CTD	611.
RGD	621033. Opn1sw.
Phylogenomic databases
eggNOG	roNOG13407.
GeneTree	ENSGT00550000074069.
HOVERGEN	HBG107442.
InParanoid	Q63652.
OrthoDB	EOG4HQDK2.
Gene expression databases
ArrayExpress	Q63652.
Genevestigator	Q63652.
GermOnline	ENSRNOG00000006874. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001760. Opsin. IPR001521. Opsin_blue. [Graphical view]
KO	K04252.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN. PR00574. OPSINBLUE.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615156.

Entry information

Entry name

OPSB_RAT

Accession

Primary (citable) accession number: Q63652
Secondary accession number(s): O70363

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2006
Last modified:	November 16, 2011
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program