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Q63651

- RK_RAT

UniProt

Q63651 - RK_RAT

Protein

Rhodopsin kinase

Gene

Grk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.

    Catalytic activityi

    ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei219 – 2191ATPPROSITE-ProRule annotation
    Active sitei317 – 3171Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi196 – 2049ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. G-protein coupled receptor kinase activity Source: InterPro
    3. rhodopsin kinase activity Source: RGD

    GO - Biological processi

    1. protein autophosphorylation Source: RGD
    2. regulation of rhodopsin mediated signaling pathway Source: UniProtKB
    3. response to drug Source: RGD
    4. response to light stimulus Source: RGD
    5. rhodopsin mediated signaling pathway Source: RGD
    6. termination of G-protein coupled receptor signaling pathway Source: InterPro
    7. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.14. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rhodopsin kinase (EC:2.7.11.14)
    Short name:
    RK
    Alternative name(s):
    G protein-coupled receptor kinase 1
    Gene namesi
    Name:Grk1
    Synonyms:Rhok
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619712. Grk1.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 561561Rhodopsin kinasePRO_0000024379Add
    BLAST
    Propeptidei562 – 5643Removed in mature formBy similarityPRO_0000024380

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei5 – 51PhosphoserineBy similarity
    Modified residuei8 – 81PhosphothreonineBy similarity
    Modified residuei21 – 211Phosphoserine; by PKA and autocatalysis1 Publication
    Modified residuei491 – 4911Phosphoserine; by autocatalysisBy similarity
    Modified residuei492 – 4921Phosphothreonine; by autocatalysisBy similarity
    Modified residuei561 – 5611Cysteine methyl esterBy similarity
    Lipidationi561 – 5611S-farnesyl cysteineBy similarity

    Post-translational modificationi

    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 By similarity. Phosphorylation at Ser-21 is regulated by light and activated by cAMP.By similarity1 Publication
    Farnesylation is required for full activity.By similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    PaxDbiQ63651.
    PRIDEiQ63651.

    PTM databases

    PhosphoSiteiQ63651.

    Expressioni

    Tissue specificityi

    Retinal-specific. Expressed in rods and cones cells.1 Publication

    Gene expression databases

    GenevestigatoriQ63651.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000024999.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63651.
    SMRiQ63651. Positions 30-536.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini58 – 175118RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini190 – 455266Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini456 – 52166AGC-kinase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 189189N-terminalAdd
    BLAST
    Regioni456 – 564109C-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000006742.
    HOVERGENiHBG004532.
    InParanoidiQ63651.
    KOiK00909.
    PhylomeDBiQ63651.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q63651-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFGSLETVV ANSAFIAARG SFDGSSTPSS RDKKYLAKLR LPPLSKCEGL    50
    RDSISLEFDN LCSEQPIGKR LFQQFLKTDE RHVPALELWK DIEDYDTADD 100
    DLRPQKAQAI LAEYLDPQGT LFCNFLDQGM VARVKEGPTG SQDGLFQPLL 150
    QATLEHLSQG PFQEYLGSLY FLRFLQWKWL EAQPIGEDWF LDFRVLGKGG 200
    FGEVSACQMK ATGKMYACKK LNKKRLKKRK GYQGAIVEKR ILAKVHSRFI 250
    VSLAYAFETK TDLCLVMTIM NGGDVRYHIY NVDEENPGFP EPRAIYYTAQ 300
    IISGLEHLHQ RRIVYRDLKP ENVLLDNDGN IRISDLGLAV ELKEGQNKTK 350
    GYAGTPGFMA PELLRGEEYD FSVDYFALGV TLYEMIAARG PFRARGEKVE 400
    NKELKQRIIS EPVKYPEKFS QASKDFCEQL LEKDPEKRLG FRDGTCDALR 450
    ANVLFKDISW RQLEAGMLIP PFIPDSRTVY AKNIQDVGAF STVKGVVFDK 500
    ADTEFFQEFA SGNCSIPWQE EMIETGFFGD LNVWRPDGQM PDDMKGITVE 550
    EAAPTAKSGM CLIS 564
    Length:564
    Mass (Da):63,769
    Last modified:November 1, 1997 - v1
    Checksum:i17E05784E6D1ED00
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U63971 mRNA. Translation: AAB05930.1.
    RefSeqiNP_112358.1. NM_031096.1.
    UniGeneiRn.10548.

    Genome annotation databases

    GeneIDi81760.
    KEGGirno:81760.
    UCSCiRGD:619712. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U63971 mRNA. Translation: AAB05930.1 .
    RefSeqi NP_112358.1. NM_031096.1.
    UniGenei Rn.10548.

    3D structure databases

    ProteinModelPortali Q63651.
    SMRi Q63651. Positions 30-536.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000024999.

    PTM databases

    PhosphoSitei Q63651.

    Proteomic databases

    PaxDbi Q63651.
    PRIDEi Q63651.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 81760.
    KEGGi rno:81760.
    UCSCi RGD:619712. rat.

    Organism-specific databases

    CTDi 6011.
    RGDi 619712. Grk1.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000006742.
    HOVERGENi HBG004532.
    InParanoidi Q63651.
    KOi K00909.
    PhylomeDBi Q63651.

    Enzyme and pathway databases

    BRENDAi 2.7.11.14. 5301.

    Miscellaneous databases

    NextBioi 615542.

    Gene expression databases

    Genevestigatori Q63651.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and localization of rhodopsin kinase in the mammalian pineal."
      Zhao X., Haeseleer F., Fariss R.N., Huang J., Baehr W., Milam A.H., Palczewski K.
      Vis. Neurosci. 14:225-232(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Retina.
    2. "Species-specific differences in expression of G-protein-coupled receptor kinase (GRK) 7 and GRK1 in mammalian cone photoreceptor cells: implications for cone cell phototransduction."
      Weiss E.R., Ducceschi M.H., Horner T.J., Li A., Craft C.M., Osawa S.
      J. Neurosci. 21:9175-9184(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    3. "Phosphorylation of G protein-coupled receptor kinase 1 (GRK1) is regulated by light but independent of phototransduction in rod photoreceptors."
      Osawa S., Jo R., Xiong Y., Reidel B., Tserentsoodol N., Arshavsky V.Y., Iuvone P.M., Weiss E.R.
      J. Biol. Chem. 286:20923-20929(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-31, IDENTIFICATION BY MASS SPECTROMETRY, PHOSPHORYLATION AT SER-21.

    Entry informationi

    Entry nameiRK_RAT
    AccessioniPrimary (citable) accession number: Q63651
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3