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Q63651

- RK_RAT

UniProt

Q63651 - RK_RAT

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Protein

Rhodopsin kinase

Gene
Grk1, Rhok
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.

Catalytic activityi

ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191ATP By similarity
Active sitei317 – 3171Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi196 – 2049ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. G-protein coupled receptor kinase activity Source: InterPro
  3. rhodopsin kinase activity Source: RGD

GO - Biological processi

  1. protein autophosphorylation Source: RGD
  2. regulation of rhodopsin mediated signaling pathway Source: UniProtKB
  3. response to drug Source: RGD
  4. response to light stimulus Source: RGD
  5. rhodopsin mediated signaling pathway Source: RGD
  6. termination of G-protein coupled receptor signaling pathway Source: InterPro
  7. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.14. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhodopsin kinase (EC:2.7.11.14)
Short name:
RK
Alternative name(s):
G protein-coupled receptor kinase 1
Gene namesi
Name:Grk1
Synonyms:Rhok
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619712. Grk1.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Rhodopsin kinasePRO_0000024379Add
BLAST
Propeptidei562 – 5643Removed in mature form By similarityPRO_0000024380

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51Phosphoserine By similarity
Modified residuei8 – 81Phosphothreonine By similarity
Modified residuei21 – 211Phosphoserine; by PKA and autocatalysis Inferred
Modified residuei491 – 4911Phosphoserine; by autocatalysis By similarity
Modified residuei492 – 4921Phosphothreonine; by autocatalysis By similarity
Modified residuei561 – 5611Cysteine methyl ester By similarity
Lipidationi561 – 5611S-farnesyl cysteine By similarity

Post-translational modificationi

Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 By similarity. Phosphorylation at Ser-21 is regulated by light and activated by cAMP.1 Publication
Farnesylation is required for full activity By similarity.

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ63651.
PRIDEiQ63651.

PTM databases

PhosphoSiteiQ63651.

Expressioni

Tissue specificityi

Retinal-specific. Expressed in rods and cones cells.1 Publication

Gene expression databases

GenevestigatoriQ63651.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024999.

Structurei

3D structure databases

ProteinModelPortaliQ63651.
SMRiQ63651. Positions 30-536.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 175118RGSAdd
BLAST
Domaini190 – 455266Protein kinaseAdd
BLAST
Domaini456 – 52166AGC-kinase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 189189N-terminalAdd
BLAST
Regioni456 – 564109C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 RGS domain.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ63651.
KOiK00909.
PhylomeDBiQ63651.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63651-1 [UniParc]FASTAAdd to Basket

« Hide

MDFGSLETVV ANSAFIAARG SFDGSSTPSS RDKKYLAKLR LPPLSKCEGL    50
RDSISLEFDN LCSEQPIGKR LFQQFLKTDE RHVPALELWK DIEDYDTADD 100
DLRPQKAQAI LAEYLDPQGT LFCNFLDQGM VARVKEGPTG SQDGLFQPLL 150
QATLEHLSQG PFQEYLGSLY FLRFLQWKWL EAQPIGEDWF LDFRVLGKGG 200
FGEVSACQMK ATGKMYACKK LNKKRLKKRK GYQGAIVEKR ILAKVHSRFI 250
VSLAYAFETK TDLCLVMTIM NGGDVRYHIY NVDEENPGFP EPRAIYYTAQ 300
IISGLEHLHQ RRIVYRDLKP ENVLLDNDGN IRISDLGLAV ELKEGQNKTK 350
GYAGTPGFMA PELLRGEEYD FSVDYFALGV TLYEMIAARG PFRARGEKVE 400
NKELKQRIIS EPVKYPEKFS QASKDFCEQL LEKDPEKRLG FRDGTCDALR 450
ANVLFKDISW RQLEAGMLIP PFIPDSRTVY AKNIQDVGAF STVKGVVFDK 500
ADTEFFQEFA SGNCSIPWQE EMIETGFFGD LNVWRPDGQM PDDMKGITVE 550
EAAPTAKSGM CLIS 564
Length:564
Mass (Da):63,769
Last modified:November 1, 1997 - v1
Checksum:i17E05784E6D1ED00
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U63971 mRNA. Translation: AAB05930.1.
RefSeqiNP_112358.1. NM_031096.1.
UniGeneiRn.10548.

Genome annotation databases

GeneIDi81760.
KEGGirno:81760.
UCSCiRGD:619712. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U63971 mRNA. Translation: AAB05930.1 .
RefSeqi NP_112358.1. NM_031096.1.
UniGenei Rn.10548.

3D structure databases

ProteinModelPortali Q63651.
SMRi Q63651. Positions 30-536.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000024999.

PTM databases

PhosphoSitei Q63651.

Proteomic databases

PaxDbi Q63651.
PRIDEi Q63651.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 81760.
KEGGi rno:81760.
UCSCi RGD:619712. rat.

Organism-specific databases

CTDi 6011.
RGDi 619712. Grk1.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000006742.
HOVERGENi HBG004532.
InParanoidi Q63651.
KOi K00909.
PhylomeDBi Q63651.

Enzyme and pathway databases

BRENDAi 2.7.11.14. 5301.

Miscellaneous databases

NextBioi 615542.

Gene expression databases

Genevestigatori Q63651.

Family and domain databases

InterProi IPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR00717. GPCRKINASE.
SMARTi SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and localization of rhodopsin kinase in the mammalian pineal."
    Zhao X., Haeseleer F., Fariss R.N., Huang J., Baehr W., Milam A.H., Palczewski K.
    Vis. Neurosci. 14:225-232(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Retina.
  2. "Species-specific differences in expression of G-protein-coupled receptor kinase (GRK) 7 and GRK1 in mammalian cone photoreceptor cells: implications for cone cell phototransduction."
    Weiss E.R., Ducceschi M.H., Horner T.J., Li A., Craft C.M., Osawa S.
    J. Neurosci. 21:9175-9184(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. "Phosphorylation of G protein-coupled receptor kinase 1 (GRK1) is regulated by light but independent of phototransduction in rod photoreceptors."
    Osawa S., Jo R., Xiong Y., Reidel B., Tserentsoodol N., Arshavsky V.Y., Iuvone P.M., Weiss E.R.
    J. Biol. Chem. 286:20923-20929(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-31, IDENTIFICATION BY MASS SPECTROMETRY, PHOSPHORYLATION AT SER-21.

Entry informationi

Entry nameiRK_RAT
AccessioniPrimary (citable) accession number: Q63651
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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