Reviewed, UniProtKB/Swiss-Prot Q63651 (RK_RAT)

Names and origin

Protein names

Recommended name:
    Rhodopsin kinase
      Short name=RK
    EC=2.7.11.14
Alternative name(s):
    G protein-coupled receptor kinase 1

Gene names

Name:	Grk1
Synonyms:	Rhok

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates rhodopsin thereby initiating its deactivation.
Catalytic activity	ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.
Subcellular location	Membrane; Lipid-anchor.
Post-translational modification	Autophosphorylated By similarity. Farnesylation is required for full activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Gene Ontology (GO)
Biological process	protein amino acid autophosphorylation Inferred from direct assay. Source: RGD response to drug Inferred from expression pattern. Source: RGD rhodopsin mediated phototransduction Ref.1 Traceable author statement. Source: RGD
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: InterPro rhodopsin kinase activity Ref.1 Inferred from direct assay. Source: RGD signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Rhodopsin kinase		PRO_0000024379
	Propeptide	562 – 564	3	Removed in mature form By similarity		PRO_0000024380
Regions
	Domain	58 – 175	118	RGS
	Domain	190 – 455	266	Protein kinase
	Domain	456 – 521	66	AGC-kinase C-terminal
	Nucleotide binding	196 – 204	9	ATP By similarity
	Region	1 – 189	189	N-terminal
	Region	456 – 564	109	C-terminal
Sites
	Active site	317	1	Proton acceptor By similarity
	Binding site	219	1	ATP By similarity
Amino acid modifications
	Modified residue	21	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	491	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	492	1	Phosphothreonine; by autocatalysis By similarity
	Modified residue	561	1	Cysteine methyl ester By similarity
	Lipidation	561	1	S-farnesyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63651-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 17E05784E6D1ED00	FASTA	564	63,769
	10 20 30 40 50 60 MDFGSLETVV ANSAFIAARG SFDGSSTPSS RDKKYLAKLR LPPLSKCEGL RDSISLEFDN 70 80 90 100 110 120 LCSEQPIGKR LFQQFLKTDE RHVPALELWK DIEDYDTADD DLRPQKAQAI LAEYLDPQGT 130 140 150 160 170 180 LFCNFLDQGM VARVKEGPTG SQDGLFQPLL QATLEHLSQG PFQEYLGSLY FLRFLQWKWL 190 200 210 220 230 240 EAQPIGEDWF LDFRVLGKGG FGEVSACQMK ATGKMYACKK LNKKRLKKRK GYQGAIVEKR 250 260 270 280 290 300 ILAKVHSRFI VSLAYAFETK TDLCLVMTIM NGGDVRYHIY NVDEENPGFP EPRAIYYTAQ 310 320 330 340 350 360 IISGLEHLHQ RRIVYRDLKP ENVLLDNDGN IRISDLGLAV ELKEGQNKTK GYAGTPGFMA 370 380 390 400 410 420 PELLRGEEYD FSVDYFALGV TLYEMIAARG PFRARGEKVE NKELKQRIIS EPVKYPEKFS 430 440 450 460 470 480 QASKDFCEQL LEKDPEKRLG FRDGTCDALR ANVLFKDISW RQLEAGMLIP PFIPDSRTVY 490 500 510 520 530 540 AKNIQDVGAF STVKGVVFDK ADTEFFQEFA SGNCSIPWQE EMIETGFFGD LNVWRPDGQM 550 560 PDDMKGITVE EAAPTAKSGM CLIS

References

[1]	"Molecular cloning and localization of rhodopsin kinase in the mammalian pineal." Zhao X., Haeseleer F., Fariss R.N., Huang J., Baehr W., Milam A.H., Palczewski K. Vis. Neurosci. 14:225-232(1997) [PubMed: 9147475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63971 mRNA. Translation: AAB05930.1.
IPI	IPI00211058.
RefSeq	NP_112358.1.
UniGene	Rn.10548
3D structure databases
SMR	Q63651. Positions 30-536.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63651.
PTM databases
PhosphoSite	Q63651.
Proteomic databases
PRIDE	Q63651.
Genome annotation databases
Ensembl	ENSRNOT00000024999; ENSRNOP00000024999; ENSRNOG00000018430; Rattus norvegicus. [Genome view]
GeneID	81760.
KEGG	rno:81760.
UCSC	NM_031096. rat.
Organism-specific databases
CTD	81760.
RGD	619712. Grk1.
Phylogenomic databases
eggNOG	roNOG15472.
HOVERGEN	Q63651.
InParanoid	Q63651.
Enzyme and pathway databases
BRENDA	2.7.11.14. 248.
Gene expression databases
Genevestigator	Q63651.
GermOnline	ENSRNOG00000018430. Rattus norvegicus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615542.

Entry information

Entry name

RK_RAT

Accession

Primary (citable) accession number: Q63651

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)