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Q63639 (AL1A2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Retinal dehydrogenase 2

Short name=RALDH 2
Short name=RalDH2
EC=1.2.1.36
Alternative name(s):
Aldehyde dehydrogenase family 1 member A2
Retinaldehyde-specific dehydrogenase type 2
Short name=RALDH(II)
Gene names
Name:Aldh1a2
Synonyms:Raldh2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length518 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently.

Catalytic activity

Retinal + NAD+ + H2O = retinoate + NADH.

Pathway

Cofactor metabolism; retinol metabolism.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Tissue specificity

Found in testis and less abundantly in lung, brain, heart, liver and kidney.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Sequence caution

The sequence AAC52637.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_process9-cis-retinoic acid biosynthetic process

Inferred from electronic annotation. Source: Ensembl

anterior/posterior pattern specification

Inferred from electronic annotation. Source: Ensembl

blood vessel development

Inferred from electronic annotation. Source: Ensembl

cardiac muscle tissue development

Inferred from electronic annotation. Source: Ensembl

cellular response to retinoic acid

Inferred from electronic annotation. Source: Ensembl

determination of bilateral symmetry

Inferred from electronic annotation. Source: Ensembl

embryonic camera-type eye development

Inferred from electronic annotation. Source: Ensembl

embryonic digestive tract development

Inferred from electronic annotation. Source: Ensembl

embryonic forelimb morphogenesis

Inferred from electronic annotation. Source: Ensembl

face development

Inferred from electronic annotation. Source: Ensembl

heart morphogenesis

Inferred from electronic annotation. Source: Ensembl

hindbrain development

Inferred from electronic annotation. Source: Ensembl

kidney development

Inferred from expression pattern PubMed 15567713. Source: RGD

liver development

Inferred from expression pattern PubMed 14729401. Source: RGD

lung development

Inferred from electronic annotation. Source: Ensembl

midgut development

Inferred from expression pattern PubMed 15964596. Source: RGD

morphogenesis of embryonic epithelium

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

neural crest cell development

Inferred from electronic annotation. Source: Ensembl

neural tube development

Inferred from electronic annotation. Source: Ensembl

neuron differentiation

Inferred from electronic annotation. Source: Ensembl

pancreas development

Inferred from electronic annotation. Source: Ensembl

pituitary gland development

Inferred from expression pattern PubMed 17180597. Source: RGD

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

proximal/distal pattern formation

Inferred from electronic annotation. Source: Ensembl

regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

response to cytokine

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from expression pattern PubMed 15205379. Source: RGD

response to vitamin A

Inferred from expression pattern PubMed 11169459. Source: RGD

retinal metabolic process

Inferred from electronic annotation. Source: Ensembl

retinoic acid biosynthetic process

Inferred from mutant phenotype PubMed 21138835. Source: RGD

retinoic acid metabolic process

Inferred from direct assay PubMed 12547725. Source: RGD

retinoic acid receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

retinol metabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

ureter maturation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytosol

Inferred from direct assay PubMed 12547725. Source: RGD

nucleus

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from direct assay PubMed 21138835. Source: RGD

   Molecular_function3-chloroallyl aldehyde dehydrogenase activity

Inferred from electronic annotation. Source: Ensembl

retinal binding

Inferred from direct assay Ref.2. Source: RGD

retinal dehydrogenase activity

Inferred from direct assay PubMed 12547725. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 518518Retinal dehydrogenase 2
PRO_0000056424

Regions

Nucleotide binding263 – 2686NAD By similarity

Sites

Active site2861Proton acceptor
Active site3201Nucleophile
Site1871Transition state stabilizer

Secondary structure

.................................................................................. 518
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q63639 [UniParc].

Last modified March 7, 2006. Version 2.
Checksum: 2BDDAABB0D2D066C

FASTA51856,640
        10         20         30         40         50         60 
MTSSEIAMPG EVKADPAALM ASLQLLPSPT PNLEIKYTKI FINNEWQNSE SGRVFPVCNP 

        70         80         90        100        110        120 
ATGEQVCEVQ EADKVDIDKA VQAARLAFSL GSVWRRMDAS ERGRLLDKLA DLVERDRATL 

       130        140        150        160        170        180 
ATMESLNGGK PFLQAFYIDL QGVIKTLRYY AGWADKIHGM TIPVDGDYFT FTRHEPIGVC 

       190        200        210        220        230        240 
GQIIPWNFPL LMFTWKIAPA LCCGNTVVIK PAEQTPLSAL YMGALIKEAG FPPGVVNILP 

       250        260        270        280        290        300 
GYGPTAGAAI ASHIGIDKIA FTGSTEVGKL IQEAAGRSNL KRVTLELGGK SPNIIFADAD 

       310        320        330        340        350        360 
LDYAVEQAHQ GVFFNQGQCC TAGSRIFVEE SIYEEFVKRS VERAKRRIVG SPFDPTTEQG 

       370        380        390        400        410        420 
PQIDKKQYNK ILELIQSGVA EGAKLECGGK GLGRKGFFIE PTVFSNVTDD MRIAKEEIFG 

       430        440        450        460        470        480 
PVQEILRFKT MDEVIERANN SDFGLVAAVF TNDINKALMV SSAMQAGTVW INCYNALNAQ 

       490        500        510 
SPFGGFKMSG NGREMGEFGL REYSEVKTVT VKIPQKNS 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[2]"Cloning of a cDNA encoding an aldehyde dehydrogenase and its expression in Escherichia coli. Recognition of retinal as substrate."
Wang X., Penzes P., Napoli J.L.
J. Biol. Chem. 271:16288-16293(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-518.
Tissue: Testis.
[3]"The structure of retinal dehydrogenase type II at 2.7 A resolution: implications for retinal specificity."
Lamb A.L., Newcomer M.E.
Biochemistry 38:6003-6011(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 20-518.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC098910 mRNA. Translation: AAH98910.1.
U60063 mRNA. Translation: AAC52637.1. Different initiation.
RefSeqNP_446348.2. NM_053896.2.
UniGeneRn.10514.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BI9X-ray2.70A/B/C/D20-518[»]
ProteinModelPortalQ63639.
SMRQ63639. Positions 20-518.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ63639.
PRIDEQ63639.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000021757; ENSRNOP00000021757; ENSRNOG00000016042.
GeneID116676.
KEGGrno:116676.
UCSCRGD:620250. rat.

Organism-specific databases

CTD8854.
RGD620250. Aldh1a2.

Phylogenomic databases

eggNOGCOG1012.
GeneTreeENSGT00550000074289.
HOGENOMHOG000271505.
HOVERGENHBG000097.
InParanoidQ63639.
KOK07249.
OMAWINCFNA.
OrthoDBEOG7PS1F7.
PhylomeDBQ63639.
TreeFamTF300455.

Enzyme and pathway databases

BRENDA1.2.1.36. 5301.
UniPathwayUPA00912.

Gene expression databases

GenevestigatorQ63639.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ63639.
NextBio619506.
PROQ63639.

Entry information

Entry nameAL1A2_RAT
AccessionPrimary (citable) accession number: Q63639
Secondary accession number(s): Q4FZY8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 7, 2006
Last modified: June 11, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways