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Q63639

- AL1A2_RAT

UniProt

Q63639 - AL1A2_RAT

Protein

Retinal dehydrogenase 2

Gene

Aldh1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently.

    Catalytic activityi

    Retinal + NAD+ + H2O = retinoate + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei187 – 1871Transition state stabilizer
    Active sitei286 – 2861Proton acceptor
    Active sitei320 – 3201Nucleophile

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi263 – 2686NADBy similarity

    GO - Molecular functioni

    1. 3-chloroallyl aldehyde dehydrogenase activity Source: Ensembl
    2. retinal binding Source: RGD
    3. retinal dehydrogenase activity Source: RGD

    GO - Biological processi

    1. 9-cis-retinoic acid biosynthetic process Source: Ensembl
    2. anterior/posterior pattern specification Source: Ensembl
    3. blood vessel development Source: Ensembl
    4. cardiac muscle tissue development Source: Ensembl
    5. cellular response to retinoic acid Source: Ensembl
    6. determination of bilateral symmetry Source: Ensembl
    7. embryonic camera-type eye development Source: Ensembl
    8. embryonic digestive tract development Source: Ensembl
    9. embryonic forelimb morphogenesis Source: Ensembl
    10. face development Source: Ensembl
    11. heart morphogenesis Source: Ensembl
    12. hindbrain development Source: Ensembl
    13. kidney development Source: RGD
    14. liver development Source: RGD
    15. lung development Source: Ensembl
    16. midgut development Source: RGD
    17. morphogenesis of embryonic epithelium Source: Ensembl
    18. negative regulation of cell proliferation Source: Ensembl
    19. neural crest cell development Source: Ensembl
    20. neural tube development Source: Ensembl
    21. neuron differentiation Source: Ensembl
    22. pancreas development Source: Ensembl
    23. pituitary gland development Source: RGD
    24. positive regulation of apoptotic process Source: Ensembl
    25. positive regulation of cell proliferation Source: Ensembl
    26. positive regulation of gene expression Source: Ensembl
    27. proximal/distal pattern formation Source: Ensembl
    28. regulation of endothelial cell proliferation Source: Ensembl
    29. response to cytokine Source: Ensembl
    30. response to estradiol Source: RGD
    31. response to vitamin A Source: RGD
    32. retinal metabolic process Source: Ensembl
    33. retinoic acid biosynthetic process Source: RGD
    34. retinoic acid metabolic process Source: RGD
    35. retinoic acid receptor signaling pathway Source: Ensembl
    36. retinol metabolic process Source: UniProtKB-UniPathway
    37. ureter maturation Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BRENDAi1.2.1.36. 5301.
    UniPathwayiUPA00912.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Retinal dehydrogenase 2 (EC:1.2.1.36)
    Short name:
    RALDH 2
    Short name:
    RalDH2
    Alternative name(s):
    Aldehyde dehydrogenase family 1 member A2
    Retinaldehyde-specific dehydrogenase type 2
    Short name:
    RALDH(II)
    Gene namesi
    Name:Aldh1a2
    Synonyms:Raldh2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi620250. Aldh1a2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD
    2. nucleus Source: Ensembl
    3. perinuclear region of cytoplasm Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 518518Retinal dehydrogenase 2PRO_0000056424Add
    BLAST

    Proteomic databases

    PaxDbiQ63639.
    PRIDEiQ63639.

    Expressioni

    Tissue specificityi

    Found in testis and less abundantly in lung, brain, heart, liver and kidney.

    Gene expression databases

    GenevestigatoriQ63639.

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    Secondary structure

    1
    518
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi39 – 424
    Beta strandi45 – 473
    Beta strandi54 – 585
    Turni60 – 623
    Beta strandi65 – 706
    Helixi74 – 8815
    Beta strandi89 – 913
    Helixi93 – 964
    Helixi99 – 11517
    Helixi117 – 12812
    Helixi132 – 1376
    Helixi139 – 15113
    Turni152 – 1554
    Beta strandi159 – 1624
    Beta strandi165 – 17612
    Beta strandi179 – 1835
    Beta strandi186 – 1883
    Helixi189 – 20214
    Beta strandi206 – 2105
    Helixi217 – 22913
    Beta strandi235 – 2384
    Turni243 – 2453
    Helixi246 – 2527
    Beta strandi258 – 2636
    Helixi265 – 27713
    Beta strandi282 – 2865
    Beta strandi293 – 2953
    Helixi301 – 31313
    Helixi314 – 3174
    Beta strandi326 – 3294
    Helixi330 – 34617
    Helixi365 – 37915
    Turni380 – 3823
    Beta strandi384 – 3874
    Beta strandi393 – 3964
    Beta strandi402 – 4065
    Helixi412 – 4154
    Beta strandi420 – 4289
    Helixi431 – 4399
    Beta strandi445 – 4506
    Helixi454 – 46310
    Beta strandi467 – 4726
    Helixi499 – 5013
    Beta strandi504 – 5129

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BI9X-ray2.70A/B/C/D20-518[»]
    ProteinModelPortaliQ63639.
    SMRiQ63639. Positions 20-518.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ63639.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG1012.
    GeneTreeiENSGT00550000074289.
    HOGENOMiHOG000271505.
    HOVERGENiHBG000097.
    InParanoidiQ63639.
    KOiK07249.
    OMAiWINCFNA.
    OrthoDBiEOG7PS1F7.
    PhylomeDBiQ63639.
    TreeFamiTF300455.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q63639-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSSEIAMPG EVKADPAALM ASLQLLPSPT PNLEIKYTKI FINNEWQNSE    50
    SGRVFPVCNP ATGEQVCEVQ EADKVDIDKA VQAARLAFSL GSVWRRMDAS 100
    ERGRLLDKLA DLVERDRATL ATMESLNGGK PFLQAFYIDL QGVIKTLRYY 150
    AGWADKIHGM TIPVDGDYFT FTRHEPIGVC GQIIPWNFPL LMFTWKIAPA 200
    LCCGNTVVIK PAEQTPLSAL YMGALIKEAG FPPGVVNILP GYGPTAGAAI 250
    ASHIGIDKIA FTGSTEVGKL IQEAAGRSNL KRVTLELGGK SPNIIFADAD 300
    LDYAVEQAHQ GVFFNQGQCC TAGSRIFVEE SIYEEFVKRS VERAKRRIVG 350
    SPFDPTTEQG PQIDKKQYNK ILELIQSGVA EGAKLECGGK GLGRKGFFIE 400
    PTVFSNVTDD MRIAKEEIFG PVQEILRFKT MDEVIERANN SDFGLVAAVF 450
    TNDINKALMV SSAMQAGTVW INCYNALNAQ SPFGGFKMSG NGREMGEFGL 500
    REYSEVKTVT VKIPQKNS 518
    Length:518
    Mass (Da):56,640
    Last modified:March 7, 2006 - v2
    Checksum:i2BDDAABB0D2D066C
    GO

    Sequence cautioni

    The sequence AAC52637.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098910 mRNA. Translation: AAH98910.1.
    U60063 mRNA. Translation: AAC52637.1. Different initiation.
    RefSeqiNP_446348.2. NM_053896.2.
    UniGeneiRn.10514.

    Genome annotation databases

    EnsembliENSRNOT00000021757; ENSRNOP00000021757; ENSRNOG00000016042.
    GeneIDi116676.
    KEGGirno:116676.
    UCSCiRGD:620250. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098910 mRNA. Translation: AAH98910.1 .
    U60063 mRNA. Translation: AAC52637.1 . Different initiation.
    RefSeqi NP_446348.2. NM_053896.2.
    UniGenei Rn.10514.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BI9 X-ray 2.70 A/B/C/D 20-518 [» ]
    ProteinModelPortali Q63639.
    SMRi Q63639. Positions 20-518.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q63639.
    PRIDEi Q63639.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000021757 ; ENSRNOP00000021757 ; ENSRNOG00000016042 .
    GeneIDi 116676.
    KEGGi rno:116676.
    UCSCi RGD:620250. rat.

    Organism-specific databases

    CTDi 8854.
    RGDi 620250. Aldh1a2.

    Phylogenomic databases

    eggNOGi COG1012.
    GeneTreei ENSGT00550000074289.
    HOGENOMi HOG000271505.
    HOVERGENi HBG000097.
    InParanoidi Q63639.
    KOi K07249.
    OMAi WINCFNA.
    OrthoDBi EOG7PS1F7.
    PhylomeDBi Q63639.
    TreeFami TF300455.

    Enzyme and pathway databases

    UniPathwayi UPA00912 .
    BRENDAi 1.2.1.36. 5301.

    Miscellaneous databases

    EvolutionaryTracei Q63639.
    NextBioi 619506.
    PROi Q63639.

    Gene expression databases

    Genevestigatori Q63639.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    2. "Cloning of a cDNA encoding an aldehyde dehydrogenase and its expression in Escherichia coli. Recognition of retinal as substrate."
      Wang X., Penzes P., Napoli J.L.
      J. Biol. Chem. 271:16288-16293(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-518.
      Tissue: Testis.
    3. "The structure of retinal dehydrogenase type II at 2.7 A resolution: implications for retinal specificity."
      Lamb A.L., Newcomer M.E.
      Biochemistry 38:6003-6011(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 20-518.

    Entry informationi

    Entry nameiAL1A2_RAT
    AccessioniPrimary (citable) accession number: Q63639
    Secondary accession number(s): Q4FZY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3