Q63635 (STX6_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-6

Gene names

Name:

Stx6

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in intracellular vesicle trafficking.
Subunit structure	Interacts with VPS45A and GOPC By similarity. Identified in a complex containing STX6, STX13, VAMP4 and VTI1A By similarity. Binds EEA1. Interacts with MARCH2 and MARCH3. Ref.3 Ref.4 Ref.5
Subcellular location	Golgi apparatus membrane; Single-pass type IV membrane protein By similarity.
Tissue specificity	Widely expressed, with relatively higher expression in brain, lung and kidney.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi vesicle transport Inferred from electronic annotation. Source: InterPro intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from direct assay Ref.1. Source: RGD trans-Golgi network membrane Inferred from direct assay. Source: BHF-UCL
Molecular function	SNAP receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EEA1	Q15075	4	EBI-398854,EBI-298113	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 255

255

Syntaxin-6

PRO_0000210211

Regions

Topological domain

1 – 234

234

Cytoplasmic Potential

Transmembrane

235 – 255

21

Helical; Anchor for type IV membrane protein; Potential

Domain

163 – 225

63

t-SNARE coiled-coil homology

Coiled coil

41 – 74

34

Potential

Amino acid modifications

Modified residue

129

1

Phosphoserine By similarity

Modified residue

152

1

Phosphoserine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

255

Helix Strand Turn

Details...

Helix

6 – 31

26

Helix

40 – 72

33

Helix

75 – 78

4

Helix

82 – 108

27

Sequences

	Sequence		Length	Mass (Da)
	Q63635 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D725FB12C1DCCA74	FASTA	255	29,057
	10 20 30 40 50 60 MSMEDPFFVV KGEVQKAVNT AQGLFQRWTE LLQGPSAATR EEIDWTTNEL RNNLRSIEWD 70 80 90 100 110 120 LEDLDETISI VEANPRKFNL DATELSIRKA FITSTRQIVR DMKDQMSASS VQALAERKNR 130 140 150 160 170 180 QALLGDSSSQ NWDAGVTDRY GRLDRELQLA NSHFIEEQQA QQQLIVEQQD EQLELVSGSI 190 200 210 220 230 240 GVLKNMSQRI GGELEEQAVM LDDFSHELES TQSRLDNVMK KLAKVSHMTS DRRQWCAIAI 250 LFAVLLVVLT LFLVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new syntaxin family member implicated in targeting of intracellular transport vesicles." Bock J.B., Lin R.C., Scheller R.H. J. Biol. Chem. 271:17961-17965(1996) [PubMed: 8663448] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"The Rab5 effector EEA1 interacts directly with syntaxin-6." Simonsen A., Gaullier J.-M., D'Arrigo A., Stenmark H. J. Biol. Chem. 274:28857-28860(1999) [PubMed: 10506127] [Abstract] Cited for: INTERACTION WITH EEA1.
[4]	"MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking." Nakamura N., Fukuda H., Kato A., Hirose S. Mol. Biol. Cell 16:1696-1710(2005) [PubMed: 15689499] [Abstract] Cited for: INTERACTION WITH MARCH2.
[5]	"MARCH-III is a novel component of endosomes with properties similar to those of MARCH-II." Fukuda H., Nakamura N., Hirose S. J. Biochem. 139:137-145(2006) [PubMed: 16428329] [Abstract] Cited for: INTERACTION WITH MARCH3.
[6]	"Three-dimensional structure of the amino-terminal domain of syntaxin 6, a SNAP-25 C homolog." Misura K.M., Bock J.B., Gonzalez L.C. Jr., Scheller R.H., Weis W.I. Proc. Natl. Acad. Sci. U.S.A. 99:9184-9189(2002) [PubMed: 12082176] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-110.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U56815 mRNA. Translation: AAC52709.1.
BC081769 mRNA. Translation: AAH81769.1.

IPI

IPI00211026.

RefSeq

NP_113853.1. NM_031665.2.

UniGene

Rn.100217.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LVF	X-ray	2.10	A/B	1-110	[»]

ProteinModelPortal

Q63635.

SMR

Q63635. Positions 4-109, 170-232.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q63635. 2 interactions.

MINT

MINT-1542524.

STRING

Q63635.

Proteomic databases

PRIDE

Q63635.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000035145; ENSRNOP00000037536; ENSRNOG00000003402.

GeneID

60562.

KEGG

rno:60562.

UCSC

NM_031665. rat.

Organism-specific databases

CTD

10228.

RGD

61915. Stx6.

Phylogenomic databases

eggNOG

roNOG05733.

GeneTree

ENSGT00390000008991.

HOVERGEN

HBG007194.

InParanoid

Q63635.

OrthoDB

EOG45HRZ9.

Gene expression databases

ArrayExpress

Q63635.

Genevestigator

Q63635.

GermOnline

ENSRNOG00000003402. Rattus norvegicus.

Family and domain databases

InterPro

IPR015260. Syntaxin-6_N.
IPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]

KO

K08498.

Pfam

PF05739. SNARE. 1 hit.
PF09177. Syntaxin-6_N. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 1 hit.
[Graphical view]

SUPFAM

SSF47661. t-snare. 1 hit.

PROSITE

PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

612238.

Entry information

Entry name

STX6_RAT

Accession

Primary (citable) accession number: Q63635

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program