Solute carrier family 12 member 4 - Rattus norvegicus (Rat)

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Q63632 (S12A4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Solute carrier family 12 member 4

Alternative name(s):
Electroneutral potassium-chloride cotransporter 1
Erythroid K-Cl cotransporter 1
Short name=rKCC1
Furosemide-sensitive K-Cl cotransporter

Gene names

Name:	Slc12a4
Synonyms:	Kcc1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1085 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl^- exit in NaCl absorbing epithelia By similarity.
Enzyme regulation	Inhibited by WNK3 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitous.
Post-translational modification	N-glycosylated. Ref.3
Sequence similarities	Belongs to the SLC12A transporter family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Symport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	potassium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	potassium:chloride symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1085

1085

Solute carrier family 12 member 4

PRO_0000178033

Regions

Topological domain

1 – 118

118

Cytoplasmic Potential

Transmembrane

119 – 139

Helical; Potential

Transmembrane

149 – 169

Helical; Potential

Topological domain

170 – 214

Cytoplasmic Potential

Transmembrane

215 – 235

Helical; Potential

Transmembrane

253 – 273

Helical; Potential

Topological domain

274 – 275

Cytoplasmic Potential

Transmembrane

276 – 296

Helical; Potential

Transmembrane

356 – 376

Helical; Potential

Topological domain

377 – 415

Cytoplasmic Potential

Transmembrane

416 – 436

Helical; Potential

Transmembrane

453 – 473

Helical; Potential

Topological domain

474 – 493

Cytoplasmic Potential

Transmembrane

494 – 514

Helical; Potential

Transmembrane

567 – 587

Helical; Potential

Topological domain

588 – 627

Cytoplasmic Potential

Transmembrane

628 – 648

Helical; Potential

Transmembrane

845 – 865

Helical; Potential

Topological domain

866 – 1085

220

Cytoplasmic Potential

Compositional bias

160 – 163

Poly-Cys

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

967

Phosphoserine By similarity

Modified residue

983

Phosphothreonine By similarity

Glycosylation

312

N-linked (GlcNAc...) Potential

Glycosylation

331

N-linked (GlcNAc...) Potential

Glycosylation

347

N-linked (GlcNAc...) Potential

Glycosylation

439

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

940

R → K in AAB18960. Ref.2

Sequence conflict

949

E → K in AAB18960. Ref.2

Sequence conflict

959

A → G Ref.2

Sequence conflict

961

R → K Ref.2

Sequence conflict

963 – 964

ES → KN Ref.2

Sequence conflict

969

E → K in AAB18960. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q63632 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 1C90B7C3F15857F9
Show »

FASTA

1,085

120,628

        10         20         30         40         50         60 
MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAEREDSDGQ GNHRENSPFL SPLDASRGND 

        70         80         90        100        110        120 
YYDRNLALFE EELDIRPKVS SLLGKLVSYT NLTQGAKEHE EAESGEGGRR RAAKAPSMGT 

       130        140        150        160        170        180 
LMGVYLPCLQ NIFGVILFLR LTWMVGTAGV LQALLIVLIC CCCTLLTAIS MSAIATNGVV 

       190        200        210        220        230        240 
PAGGSYFMIS RSLGPEFGGA VGLCFYLGTT FAAAMYILGA IEILLTYIAP PAAIFYPSGT 

       250        260        270        280        290        300 
HDMSSATLNN MRVYGTIFLT FMTLVVFVGV KYVNKFASLF LACVIISILS IYVGGIKSAF 

       310        320        330        340        350        360 
DPPVFPVCML GNRTLSRDQF DICAKTVVVD NETVATRLWT FFCHSPNLTA DSCDPYFLLN 

       370        380        390        400        410        420 
NVTEIPGIPG AAAGVLQENL WSAYLEKGEV VEKHGLPSTD TLGLKESLSL YVVADIATSF 

       430        440        450        460        470        480 
TVLVGIFFPS VTGIMAGSNR SGDLRDAQKS IPVGTILAIV TTSLVYFSSV ILFGACIEGV 

       490        500        510        520        530        540 
VLRDKYGDGV SRNLVVGTLA WPSPWVIVVG SFFSTCGAGL QSLTGAPRLL QAIAKDNIIP 

       550        560        570        580        590        600 
FLRVFGHGKA NGEPTWALLL TALIAELGIL IASLDMVAPI LSMFFLMCYL FVNLACAVQT 

       610        620        630        640        650        660 
LLRTPNWRPR FKYYHWALSF LGMSLCLALM FVSSWYYALV AMVIAGMIYK YIEYQGAEKE 

       670        680        690        700        710        720 
WGDGIRGLSL SAARYALLRL EEGPPHTKNW RPQLLVLLKL DEDLHVKYPR LLTFASQLKA 

       730        740        750        760        770        780 
GKGLTIVGSV IQGSFLESYG EAQAAEQTIK NMMEIEKVKG FCQVVVASKV REGLAHLIQS 

       790        800        810        820        830        840 
CGLGGMRHNS VVLGWPYGWR QSEDPRAWKT FIDTVRCTTA AHLALLVPKN IAFYPSNHER 

       850        860        870        880        890        900 
YLEGHIDVWW IVHDGGMLML LPFLLRQHKV WKKCRMRIFT VAQMDDNSIQ MKKDLAIFLY 

       910        920        930        940        950        960 
HLRLEAEVEV VEMHNSDISA YTYERTLMME QRSQMLRQMR LTKTERDREA QLVKDRHSAL 

       970        980        990       1000       1010       1020 
RLESLYSDEE DESVTGADKI QMTWTRDKYM AEPWDPSHAP DNFRELVHIK PDQSNVRRMH 

      1030       1040       1050       1060       1070       1080 
TAVKLNEVIV TRSHDARLVL LNMPGPPKNS EGDENYMEFL EVLTEGLERV LLVRGGGREV 


ITIYS

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References

[1]	"Molecular cloning and functional expression of the K-Cl cotransporter from rabbit, rat, and human. A new member of the cation-chloride cotransporter family." Gillen C.M., Brill S., Payne J.A., Forbush B. III J. Biol. Chem. 271:16237-16244(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	Adams L.A., Werny I., Schwartz S.M. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 842-970. Strain: Wistar Kyoto. Tissue: Aorta.
[3]	"Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation." Su W., Shmukler B.E., Chernova M.N., Stuart-Tilley A.K., de Franceschi L., Brugnara C., Alper S.L. Am. J. Physiol. 277:C899-C912(1999) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U55815 mRNA. Translation: AAC52634.1. U75396 mRNA. Translation: AAB18960.1.
IPI	IPI00211023.
PIR	T31429.
RefSeq	NP_062102.1. NM_019229.2.
UniGene	Rn.32091.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	2.A.30.5.1. cation-chloride cotransporter (CCC) family.
Proteomic databases
PaxDb	Q63632.
PRIDE	Q63632.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026730; ENSRNOP00000026730; ENSRNOG00000019651.
GeneID	29501.
KEGG	rno:29501.
UCSC	RGD:3687. rat.
Organism-specific databases
CTD	6560.
RGD	3687. Slc12a4.
Phylogenomic databases
eggNOG	COG0531.
GeneTree	ENSGT00560000076892.
HOGENOM	HOG000092644.
HOVERGEN	HBG052852.
InParanoid	Q63632.
KO	K14427.
OrthoDB	EOG476JZG.
Gene expression databases
Genevestigator	Q63632.
GermOnline	ENSRNOG00000019651. Rattus norvegicus.
Family and domain databases
InterPro	IPR004841. AA-permease_dom. IPR000622. K/Cl_cotranspt1. IPR018491. K/Cl_cotranspt_1/3. IPR000076. KCL_cotranspt. IPR004842. Na/K/Cl_cotransptS. [Graphical view]
Pfam	PF00324. AA_permease. 2 hits. PF03522. KCl_Cotrans_1. 1 hit. [Graphical view]
PRINTS	PR01081. KCLTRNSPORT. PR01082. KCLTRNSPORT1.
TIGRFAMs	TIGR00930. 2a30. 1 hit.
ProtoNet	Search...
Other
NextBio	609404.

Entry information

Entry name

S12A4_RAT

Accession

Primary (citable) accession number: Q63632
Secondary accession number(s): P70632

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 13, 2002
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents