Q63622 (DLG2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Disks large homolog 2

Alternative name(s):
Channel-associated protein of synapse-110
Short name=Chapsyn-110
Postsynaptic density protein PSD-93

Gene names

Name:	Dlg2
Synonyms:	Dlgh2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	852 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses By similarity.
Subunit structure	Interacts through its PDZ domains with NETO1. Interacts with NOS1/nNOS through second PDZ domain. Interacts with KCNJ2/Kir2.1 (via C-terminus) through one of its PDZ domains By similarity. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1. Interacts with LRFN2 and LRFN4. Interacts with FASLG By similarity. Ref.5 Ref.8 Ref.9
Subcellular location	Membrane; Lipid-anchor. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Note: Concentrated in soma and postsynaptic density of a subset of neurons. Ref.5 Ref.7
Tissue specificity	Brain. High levels in cerebellar Purkinje cells. Expressed in pyramidal cells of the Ammons's horn and granular cells of the dentate gyrus in the hippocampus as well as cerebral cortex and striatum. High levels in dorsal horn of spinal cord. Ref.4 Ref.5
Developmental stage	High levels in developing brain and spinal chord, sensory neurons of dorsal root and trigeminal ganglia, myenteric neurons of the intestine as well as in non-neuronal cells of adrenal, thymus and submandibular glands of E15 embryos. Ref.5
Post-translational modification	Palmitoylation of isoform 1 and isoform 2 is not required for targeting to postsynaptic density. Ref.6
Sequence similarities	Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Repeat SH3 domain
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB neuronal ion channel clustering Inferred from mutant phenotype Ref.1. Source: RGD receptor clustering Inferred from mutant phenotype Ref.1. Source: RGD
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from direct assay Ref.1. Source: RGD juxtaparanode region of axon Inferred from direct assay PubMed 19109503. Source: MGI membrane Inferred from direct assay Ref.1. Source: RGD neuronal cell body Inferred from direct assay Ref.1. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic density Traceable author statement PubMed 1127911. Source: UniProtKB postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein C-terminus binding Traceable author statement Ref.1. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATP2B2	Q01814-1	2	EBI-396947,EBI-1174262	From a different organism.
ATP2B4	P23634-6	2	EBI-396947,EBI-1174437	From a different organism.
KIF1B	O60333-3	3	EBI-396947,EBI-465669	From a different organism.
Map1a	P34926	4	EBI-396947,EBI-631571

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q63622-1)

Also known as: PSD-93b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q63622-2)

Also known as: PSD-93a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MFFACYCALRTNV → MICHCKVACTNNTLSLMFGC

Isoform 3 (identifier: Q63622-3)

Also known as: PSD-93c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     62-68: SHISPLK → MQHAFIP
     341-392: Missing.

Isoform 4 (identifier: Q63622-4)

Also known as: PSD-93-delta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: MFFACYCALR...VLQSHISPLK → MNAYLTKQHS...CPHGWFSPAQ

Isoform 5 (identifier: Q63622-5)

Also known as: PSD-93d;

The sequence of this isoform differs from the canonical sequence as follows:
     1-246: Missing.

Note: No experimental confirmation available.

Isoform 6 (identifier: Q63622-6)

The sequence of this isoform differs from the canonical sequence as follows:
     450-454: Missing.
     626-641: Missing.

Isoform 7 (identifier: Q63622-7)

The sequence of this isoform differs from the canonical sequence as follows:
     626-641: GDIPGLGDDGYGTKTL → GSFNDKRKKSFIFSRKFPFYKNKEQSEQETSDPE

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 852	852	Disks large homolog 2		PRO_0000094555
Regions
	Domain	98 – 184	87	PDZ 1
	Domain	193 – 279	87	PDZ 2
	Domain	421 – 501	81	PDZ 3
	Domain	536 – 606	71	SH3
	Domain	662 – 837	176	Guanylate kinase-like
Amino acid modifications
	Modified residue	28	1	Phosphoserine By similarity
	Modified residue	58	1	Phosphotyrosine By similarity
	Modified residue	62	1	Phosphoserine By similarity
	Modified residue	175	1	Phosphoserine By similarity
	Modified residue	340	1	Phosphotyrosine By similarity
	Modified residue	348	1	Phosphotyrosine By similarity
	Modified residue	365	1	Phosphoserine By similarity
	Modified residue	406	1	Phosphoserine By similarity
	Modified residue	414	1	Phosphoserine By similarity
	Modified residue	500	1	Phosphotyrosine By similarity
	Modified residue	505	1	Phosphotyrosine By similarity
	Modified residue	732	1	Phosphotyrosine By similarity
	Modified residue	737	1	Phosphotyrosine By similarity
	Lipidation	5	1	S-palmitoyl cysteine Ref.6
	Lipidation	7	1	S-palmitoyl cysteine Ref.6
Natural variations
	Alternative sequence	1 – 246	246	Missing in isoform 5.		VSP_015525
	Alternative sequence	1 – 68	68	MFFAC…ISPLK → MNAYLTKQHSCSRGSDGMDA GRGVPTLIRDAHCACGWQRN AQGLGYSSQTMPSSGPGGPA SNRTKLVTLWDSVRKSPHKT STKGKGNCGERCACPHGWFS PAQ in isoform 4.		VSP_015526
	Alternative sequence	1 – 61	61	Missing in isoform 3.		VSP_015527
	Alternative sequence	1 – 13	13	MFFAC…LRTNV → MICHCKVACTNNTLSLMFGC in isoform 2.		VSP_015528
	Alternative sequence	62 – 68	7	SHISPLK → MQHAFIP in isoform 3.		VSP_015529
	Alternative sequence	341 – 392	52	Missing in isoform 3.		VSP_015530
	Alternative sequence	450 – 454	5	Missing in isoform 6.		VSP_015531
	Alternative sequence	626 – 641	16	Missing in isoform 6.		VSP_015533
	Alternative sequence	626 – 641	16	GDIPG…GTKTL → GSFNDKRKKSFIFSRKFPFY KNKEQSEQETSDPE in isoform 7.		VSP_015532
Experimental info
	Mutagenesis	5	1	C → S: Loss of palmitoylation and targeting to postsynaptic density. Ref.6
	Mutagenesis	7	1	C → S: Loss of palmitoylation and targeting to postsynaptic density. Ref.6
	Sequence conflict	181 – 182	2	VR → IL in AAC52643. Ref.2
	Sequence conflict	228	1	I → M in AAC52643. Ref.2
	Sequence conflict	326	1	R → K in AAC52643. Ref.2
	Sequence conflict	339	1	D → E in AAB48562. Ref.3
	Sequence conflict	464 – 465	2	GD → RK in AAC52643. Ref.2
	Sequence conflict	474	1	D → H Ref.2
	Sequence conflict	476	1	R → P Ref.2
	Sequence conflict	478	1	A → D Ref.2
	Sequence conflict	484 – 486	3	AAA → LP in AAC52643. Ref.2
	Sequence conflict	506	1	A → S in AAC52643. Ref.2
	Sequence conflict	569	1	H → N in AAC52643. Ref.2
	Sequence conflict	586	1	L → Q in AAC52643. Ref.2
	Sequence conflict	627 – 630	4	DIPG → TSR Ref.5
	Sequence conflict	639	1	K → A in AAB48562. Ref.3
	Sequence conflict	726	1	F → L in AAB53243. Ref.1
	Sequence conflict	733	1	N → Y in AAC52643. Ref.2
	Sequence conflict	749	1	E → V in AAB53243. Ref.1
	Sequence conflict	756	1	L → H in AAC52643. Ref.2
	Sequence conflict	791 – 792	2	KR → NG Ref.2
	Sequence conflict	794	1	T → M Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PSD-93b) [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F8D414A8B9CF5B09	FASTA	852	94,934
	10 20 30 40 50 60 MFFACYCALR TNVKKYRYQD EDGPHDHSLP RLTHEVRGPE LVHVSEKNLS QIENVHGYVL 70 80 90 100 110 120 QSHISPLKAS PAPIIVNTDT LDTIPYVNGT EIEYEFEEIT LERGNSGLGF SIAGGTDNPH 130 140 150 160 170 180 IGDDPGIFIT KIIPGGAAAE DGRLRVNDCI LRVNEVDVSE VSHSKAVEAL KEAGSIVRLY 190 200 210 220 230 240 VRRRRPILET VVEIKLFKGP KGLGFSIAGG VGNQHIPGDN SIYVTKIIDG GAAQKDGRLQ 250 260 270 280 290 300 VGDRLLMVNN YSLEEVTHEE AVAILKNTSD VVYLKVGKPT TIYMTDPYGP PDITHSYSPP 310 320 330 340 350 360 MENHLLSGNN GTLEYKTSLP PISPGRYSPI PKHMLVEDDY TRPPEPVYST VNKLCDKPAS 370 380 390 400 410 420 PRHYSPVECD KSFLLSTPYP HYHLGLLPDS DMTSHSQHST ATRQPSVTLQ RAISLEGEPR 430 440 450 460 470 480 KVVLHKGSTG LGFNIVGGED GEGIFVSFIL AGGPADLSGE LQRGDQILSV NGIDLRGASH 490 500 510 520 530 540 EQAAAALKGA GQTVTIIAQY QPEDYARFEA KIHDLREQMM NHSMSSGSGS LRTNQKRSLY 550 560 570 580 590 600 VRAMFDYDKS KDSGLPSQGL SFKYGDILHV INASDDEWWQ ARRVILDGDS EEMGVIPSKR 610 620 630 640 650 660 RVERKERARL KTVKFNAKPG VIDSKGDIPG LGDDGYGTKT LRGQEDLILS YEPVTRQEIN 670 680 690 700 710 720 YTRPVIILGP MKDRINDDLI SEFPDKFGSC VPHTTRPKRD YEVDGRDYHF VISREQMEKD 730 740 750 760 770 780 IQEHKFIEAG QYNDNLYGTS VQSVRFVAER GKHCILDVSG NAIKRLQVAQ LYPIAIFIKP 790 800 810 820 830 840 KSLEPLMEMN KRLTEEQAKK TYDRAIKLEQ EFGEYFTAIV QGDTLEDIYN QCKLVIEEQS 850 GPFIWIPSKE KL
	Isoform 2 (PSD-93a) [UniParc]. Checksum: 4843703C194A1CE8	FASTA	859	95,585
	10 20 30 40 50 60 MICHCKVACT NNTLSLMFGC KKYRYQDEDG PHDHSLPRLT HEVRGPELVH VSEKNLSQIE 70 80 90 100 110 120 NVHGYVLQSH ISPLKASPAP IIVNTDTLDT IPYVNGTEIE YEFEEITLER GNSGLGFSIA 130 140 150 160 170 180 GGTDNPHIGD DPGIFITKII PGGAAAEDGR LRVNDCILRV NEVDVSEVSH SKAVEALKEA 190 200 210 220 230 240 GSIVRLYVRR RRPILETVVE IKLFKGPKGL GFSIAGGVGN QHIPGDNSIY VTKIIDGGAA 250 260 270 280 290 300 QKDGRLQVGD RLLMVNNYSL EEVTHEEAVA ILKNTSDVVY LKVGKPTTIY MTDPYGPPDI 310 320 330 340 350 360 THSYSPPMEN HLLSGNNGTL EYKTSLPPIS PGRYSPIPKH MLVEDDYTRP PEPVYSTVNK 370 380 390 400 410 420 LCDKPASPRH YSPVECDKSF LLSTPYPHYH LGLLPDSDMT SHSQHSTATR QPSVTLQRAI 430 440 450 460 470 480 SLEGEPRKVV LHKGSTGLGF NIVGGEDGEG IFVSFILAGG PADLSGELQR GDQILSVNGI 490 500 510 520 530 540 DLRGASHEQA AAALKGAGQT VTIIAQYQPE DYARFEAKIH DLREQMMNHS MSSGSGSLRT 550 560 570 580 590 600 NQKRSLYVRA MFDYDKSKDS GLPSQGLSFK YGDILHVINA SDDEWWQARR VILDGDSEEM 610 620 630 640 650 660 GVIPSKRRVE RKERARLKTV KFNAKPGVID SKGDIPGLGD DGYGTKTLRG QEDLILSYEP 670 680 690 700 710 720 VTRQEINYTR PVIILGPMKD RINDDLISEF PDKFGSCVPH TTRPKRDYEV DGRDYHFVIS 730 740 750 760 770 780 REQMEKDIQE HKFIEAGQYN DNLYGTSVQS VRFVAERGKH CILDVSGNAI KRLQVAQLYP 790 800 810 820 830 840 IAIFIKPKSL EPLMEMNKRL TEEQAKKTYD RAIKLEQEFG EYFTAIVQGD TLEDIYNQCK 850 LVIEEQSGPF IWIPSKEKL
	Isoform 3 (PSD-93c) [UniParc]. Checksum: 6C33B93499B8E70A	FASTA	739	81,962
	10 20 30 40 50 60 MQHAFIPASP APIIVNTDTL DTIPYVNGTE IEYEFEEITL ERGNSGLGFS IAGGTDNPHI 70 80 90 100 110 120 GDDPGIFITK IIPGGAAAED GRLRVNDCIL RVNEVDVSEV SHSKAVEALK EAGSIVRLYV 130 140 150 160 170 180 RRRRPILETV VEIKLFKGPK GLGFSIAGGV GNQHIPGDNS IYVTKIIDGG AAQKDGRLQV 190 200 210 220 230 240 GDRLLMVNNY SLEEVTHEEA VAILKNTSDV VYLKVGKPTT IYMTDPYGPP DITHSYSPPM 250 260 270 280 290 300 ENHLLSGNNG TLEYKTSLPP ISPGRYSPIP KHMLVEDDYT SHSQHSTATR QPSVTLQRAI 310 320 330 340 350 360 SLEGEPRKVV LHKGSTGLGF NIVGGEDGEG IFVSFILAGG PADLSGELQR GDQILSVNGI 370 380 390 400 410 420 DLRGASHEQA AAALKGAGQT VTIIAQYQPE DYARFEAKIH DLREQMMNHS MSSGSGSLRT 430 440 450 460 470 480 NQKRSLYVRA MFDYDKSKDS GLPSQGLSFK YGDILHVINA SDDEWWQARR VILDGDSEEM 490 500 510 520 530 540 GVIPSKRRVE RKERARLKTV KFNAKPGVID SKGDIPGLGD DGYGTKTLRG QEDLILSYEP 550 560 570 580 590 600 VTRQEINYTR PVIILGPMKD RINDDLISEF PDKFGSCVPH TTRPKRDYEV DGRDYHFVIS 610 620 630 640 650 660 REQMEKDIQE HKFIEAGQYN DNLYGTSVQS VRFVAERGKH CILDVSGNAI KRLQVAQLYP 670 680 690 700 710 720 IAIFIKPKSL EPLMEMNKRL TEEQAKKTYD RAIKLEQEFG EYFTAIVQGD TLEDIYNQCK 730 LVIEEQSGPF IWIPSKEKL
	Isoform 4 (PSD-93-delta) [UniParc]. Checksum: FBB0E9EF8F855A59	FASTA	887	97,938
	10 20 30 40 50 60 MNAYLTKQHS CSRGSDGMDA GRGVPTLIRD AHCACGWQRN AQGLGYSSQT MPSSGPGGPA 70 80 90 100 110 120 SNRTKLVTLW DSVRKSPHKT STKGKGNCGE RCACPHGWFS PAQASPAPII VNTDTLDTIP 130 140 150 160 170 180 YVNGTEIEYE FEEITLERGN SGLGFSIAGG TDNPHIGDDP GIFITKIIPG GAAAEDGRLR 190 200 210 220 230 240 VNDCILRVNE VDVSEVSHSK AVEALKEAGS IVRLYVRRRR PILETVVEIK LFKGPKGLGF 250 260 270 280 290 300 SIAGGVGNQH IPGDNSIYVT KIIDGGAAQK DGRLQVGDRL LMVNNYSLEE VTHEEAVAIL 310 320 330 340 350 360 KNTSDVVYLK VGKPTTIYMT DPYGPPDITH SYSPPMENHL LSGNNGTLEY KTSLPPISPG 370 380 390 400 410 420 RYSPIPKHML VEDDYTRPPE PVYSTVNKLC DKPASPRHYS PVECDKSFLL STPYPHYHLG 430 440 450 460 470 480 LLPDSDMTSH SQHSTATRQP SVTLQRAISL EGEPRKVVLH KGSTGLGFNI VGGEDGEGIF 490 500 510 520 530 540 VSFILAGGPA DLSGELQRGD QILSVNGIDL RGASHEQAAA ALKGAGQTVT IIAQYQPEDY 550 560 570 580 590 600 ARFEAKIHDL REQMMNHSMS SGSGSLRTNQ KRSLYVRAMF DYDKSKDSGL PSQGLSFKYG 610 620 630 640 650 660 DILHVINASD DEWWQARRVI LDGDSEEMGV IPSKRRVERK ERARLKTVKF NAKPGVIDSK 670 680 690 700 710 720 GDIPGLGDDG YGTKTLRGQE DLILSYEPVT RQEINYTRPV IILGPMKDRI NDDLISEFPD 730 740 750 760 770 780 KFGSCVPHTT RPKRDYEVDG RDYHFVISRE QMEKDIQEHK FIEAGQYNDN LYGTSVQSVR 790 800 810 820 830 840 FVAERGKHCI LDVSGNAIKR LQVAQLYPIA IFIKPKSLEP LMEMNKRLTE EQAKKTYDRA 850 860 870 880 IKLEQEFGEY FTAIVQGDTL EDIYNQCKLV IEEQSGPFIW IPSKEKL
	Isoform 5 (PSD-93d) [UniParc]. Checksum: F87FF88D002EA965	FASTA	606	68,062
	10 20 30 40 50 60 MVNNYSLEEV THEEAVAILK NTSDVVYLKV GKPTTIYMTD PYGPPDITHS YSPPMENHLL 70 80 90 100 110 120 SGNNGTLEYK TSLPPISPGR YSPIPKHMLV EDDYTRPPEP VYSTVNKLCD KPASPRHYSP 130 140 150 160 170 180 VECDKSFLLS TPYPHYHLGL LPDSDMTSHS QHSTATRQPS VTLQRAISLE GEPRKVVLHK 190 200 210 220 230 240 GSTGLGFNIV GGEDGEGIFV SFILAGGPAD LSGELQRGDQ ILSVNGIDLR GASHEQAAAA 250 260 270 280 290 300 LKGAGQTVTI IAQYQPEDYA RFEAKIHDLR EQMMNHSMSS GSGSLRTNQK RSLYVRAMFD 310 320 330 340 350 360 YDKSKDSGLP SQGLSFKYGD ILHVINASDD EWWQARRVIL DGDSEEMGVI PSKRRVERKE 370 380 390 400 410 420 RARLKTVKFN AKPGVIDSKG DIPGLGDDGY GTKTLRGQED LILSYEPVTR QEINYTRPVI 430 440 450 460 470 480 ILGPMKDRIN DDLISEFPDK FGSCVPHTTR PKRDYEVDGR DYHFVISREQ MEKDIQEHKF 490 500 510 520 530 540 IEAGQYNDNL YGTSVQSVRF VAERGKHCIL DVSGNAIKRL QVAQLYPIAI FIKPKSLEPL 550 560 570 580 590 600 MEMNKRLTEE QAKKTYDRAI KLEQEFGEYF TAIVQGDTLE DIYNQCKLVI EEQSGPFIWI PSKEKL
	Isoform 6 [UniParc]. Checksum: 0B9B45B7A317B263	FASTA	831	92,978
	10 20 30 40 50 60 MFFACYCALR TNVKKYRYQD EDGPHDHSLP RLTHEVRGPE LVHVSEKNLS QIENVHGYVL 70 80 90 100 110 120 QSHISPLKAS PAPIIVNTDT LDTIPYVNGT EIEYEFEEIT LERGNSGLGF SIAGGTDNPH 130 140 150 160 170 180 IGDDPGIFIT KIIPGGAAAE DGRLRVNDCI LRVNEVDVSE VSHSKAVEAL KEAGSIVRLY 190 200 210 220 230 240 VRRRRPILET VVEIKLFKGP KGLGFSIAGG VGNQHIPGDN SIYVTKIIDG GAAQKDGRLQ 250 260 270 280 290 300 VGDRLLMVNN YSLEEVTHEE AVAILKNTSD VVYLKVGKPT TIYMTDPYGP PDITHSYSPP 310 320 330 340 350 360 MENHLLSGNN GTLEYKTSLP PISPGRYSPI PKHMLVEDDY TRPPEPVYST VNKLCDKPAS 370 380 390 400 410 420 PRHYSPVECD KSFLLSTPYP HYHLGLLPDS DMTSHSQHST ATRQPSVTLQ RAISLEGEPR 430 440 450 460 470 480 KVVLHKGSTG LGFNIVGGED GEGIFVSFIA DLSGELQRGD QILSVNGIDL RGASHEQAAA 490 500 510 520 530 540 ALKGAGQTVT IIAQYQPEDY ARFEAKIHDL REQMMNHSMS SGSGSLRTNQ KRSLYVRAMF 550 560 570 580 590 600 DYDKSKDSGL PSQGLSFKYG DILHVINASD DEWWQARRVI LDGDSEEMGV IPSKRRVERK 610 620 630 640 650 660 ERARLKTVKF NAKPGVIDSK RGQEDLILSY EPVTRQEINY TRPVIILGPM KDRINDDLIS 670 680 690 700 710 720 EFPDKFGSCV PHTTRPKRDY EVDGRDYHFV ISREQMEKDI QEHKFIEAGQ YNDNLYGTSV 730 740 750 760 770 780 QSVRFVAERG KHCILDVSGN AIKRLQVAQL YPIAIFIKPK SLEPLMEMNK RLTEEQAKKT 790 800 810 820 830 YDRAIKLEQE FGEYFTAIVQ GDTLEDIYNQ CKLVIEEQSG PFIWIPSKEK L
	Isoform 7 [UniParc]. Checksum: A6890D04BE125927	FASTA	870	97,486
	10 20 30 40 50 60 MFFACYCALR TNVKKYRYQD EDGPHDHSLP RLTHEVRGPE LVHVSEKNLS QIENVHGYVL 70 80 90 100 110 120 QSHISPLKAS PAPIIVNTDT LDTIPYVNGT EIEYEFEEIT LERGNSGLGF SIAGGTDNPH 130 140 150 160 170 180 IGDDPGIFIT KIIPGGAAAE DGRLRVNDCI LRVNEVDVSE VSHSKAVEAL KEAGSIVRLY 190 200 210 220 230 240 VRRRRPILET VVEIKLFKGP KGLGFSIAGG VGNQHIPGDN SIYVTKIIDG GAAQKDGRLQ 250 260 270 280 290 300 VGDRLLMVNN YSLEEVTHEE AVAILKNTSD VVYLKVGKPT TIYMTDPYGP PDITHSYSPP 310 320 330 340 350 360 MENHLLSGNN GTLEYKTSLP PISPGRYSPI PKHMLVEDDY TRPPEPVYST VNKLCDKPAS 370 380 390 400 410 420 PRHYSPVECD KSFLLSTPYP HYHLGLLPDS DMTSHSQHST ATRQPSVTLQ RAISLEGEPR 430 440 450 460 470 480 KVVLHKGSTG LGFNIVGGED GEGIFVSFIL AGGPADLSGE LQRGDQILSV NGIDLRGASH 490 500 510 520 530 540 EQAAAALKGA GQTVTIIAQY QPEDYARFEA KIHDLREQMM NHSMSSGSGS LRTNQKRSLY 550 560 570 580 590 600 VRAMFDYDKS KDSGLPSQGL SFKYGDILHV INASDDEWWQ ARRVILDGDS EEMGVIPSKR 610 620 630 640 650 660 RVERKERARL KTVKFNAKPG VIDSKGSFND KRKKSFIFSR KFPFYKNKEQ SEQETSDPER 670 680 690 700 710 720 GQEDLILSYE PVTRQEINYT RPVIILGPMK DRINDDLISE FPDKFGSCVP HTTRPKRDYE 730 740 750 760 770 780 VDGRDYHFVI SREQMEKDIQ EHKFIEAGQY NDNLYGTSVQ SVRFVAERGK HCILDVSGNA 790 800 810 820 830 840 IKRLQVAQLY PIAIFIKPKS LEPLMEMNKR LTEEQAKKTY DRAIKLEQEF GEYFTAIVQG 850 860 870 DTLEDIYNQC KLVIEEQSGP FIWIPSKEKL

References

[1]	"Heteromultimerization and NMDA receptor-clustering activity of Chapsyn-110, a member of the PSD-95 family of proteins." Kim E., Cho K.-O., Rothschild A., Sheng M. Neuron 17:103-113(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha1-syntrophin mediated by PDZ domains." Brenman J.E., Chao D.S., Gee S.H., McGee A.W., Craven S.E., Santillano D.R., Wu Z., Huang F., Xia H., Peters M.F., Froehner S.C., Bredt D.S. Cell 84:757-767(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6).
[3]	Irie M., Hata Y., Takai Y. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"An alternatively spliced isoform of PSD-93/chapsyn 110 binds to the inwardly rectifying potassium channel, Kir2.1." Leyland M.L., Dart C. J. Biol. Chem. 279:43427-43436(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), TISSUE SPECIFICITY.
[5]	"Cloning and characterization of postsynaptic density 93, a nitric oxide synthase interacting protein." Brenman J.E., Christopherson K.S., Craven S.E., McGee A.W., Bredt D.S. J. Neurosci. 16:7407-7415(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 5), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH NOS1.
[6]	"Ion channel clustering by membrane-associated guanylate kinases. Differential regulation by N-terminal lipid and metal binding motifs." El-Husseini A.E., Topinka J.R., Lehrer-Graiwer J.E., Firestein B.L., Craven S.E., Aoki C., Bredt D.S. J. Biol. Chem. 275:23904-23910(2000) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF CYS-5 AND CYS-7, PALMITOYLATION AT CYS-5 AND CYS-7.
[7]	"Postsynaptic targeting of MAGUKs mediated by distinct N-terminal domains." Firestein B.L., Craven S.E., Bredt D.S. NeuroReport 11:3479-3484(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses." Ko J., Kim S., Chung H.S., Kim K., Han K., Kim H., Jun H., Kaang B.-K., Kim E. Neuron 50:233-245(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LRFN1.
[9]	"Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis." Lee H.W., Choi J., Shin H., Kim K., Yang J., Na M., Choi S.Y., Kang G.B., Eom S.H., Kim H., Kim E. J. Neurosci. 28:14546-14556(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FRMPD4.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U49049 mRNA. Translation: AAB53243.1.
U50717 mRNA. Translation: AAC52643.1.
U53368 mRNA. Translation: AAB48562.1.

IPI

IPI00327448.
IPI00650085.
IPI00650088.
IPI00650099.
IPI00650104.
IPI00650105.
IPI00650109.

PIR

T10811.

RefSeq

NP_071618.1. NM_022282.1.

UniGene

Rn.202966.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4H11	X-ray	1.67	A/B	95-188	[»]

ProteinModelPortal

Q63622.

SMR

Q63622. Positions 95-185, 190-283, 418-514, 539-852.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q63622. 8 interactions.

MINT

MINT-155119.

PTM databases

PhosphoSite

Q63622.

Proteomic databases

PaxDb

Q63622.

PRIDE

Q63622.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

64053.

KEGG

rno:64053.

UCSC

RGD:619895. rat.

Organism-specific databases

CTD

1740.

RGD

619895. Dlg2.

Phylogenomic databases

eggNOG

COG0194.

HOGENOM

HOG000232102.

HOVERGEN

HBG107814.

KO

K12075.

OrthoDB

EOG447FSN.

Gene expression databases

ArrayExpress

Q63622.

Genevestigator

Q63622.

GermOnline

ENSRNOG00000022635. Rattus norvegicus.

Family and domain databases

InterPro

IPR008144. Guanylate_kin.
IPR008145. Guanylate_kin/L-typ_Ca_channel.
IPR020590. Guanylate_kinase_CS.
IPR016313. M-assoc_guanylate_kinase.
IPR019590. MAGUK_PEST_N.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR019583. PDZ_assoc.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00625. Guanylate_kin. 1 hit.
PF10608. MAGUK_N_PEST. 1 hit.
PF00595. PDZ. 3 hits.
PF10600. PDZ_assoc. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]

PIRSF

PIRSF001741. MAGUK_DLGH. 1 hit.

SMART

SM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50156. PDZ. 3 hits.
SSF50044. SH3. 1 hit.
SSF52540. SSF52540. 1 hit.

PROSITE

PS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

612717.

Entry information

Entry name

DLG2_RAT

Accession

Primary (citable) accession number: Q63622
Secondary accession number(s): P70548, Q62939

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program