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Protein

Vacuolar protein sorting-associated protein 33A

Gene

Vps33a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 33A
Short name:
r-vps33a
Gene namesi
Name:Vps33a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620643. Vps33a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 597597Vacuolar protein sorting-associated protein 33APRO_0000206304Add
BLAST

Proteomic databases

PaxDbiQ63615.
PRIDEiQ63615.

PTM databases

PhosphoSiteiQ63615.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuaole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1 (PubMed:11382755). Interacts with RAB5C, UVRAG, STX17, MON1A and MON1B. Associates with adaptor protein complex 3 (AP-3) and clathrin (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS11Q9H2704EBI-918669,EBI-373380From a different organism.
VPS18Q9P2532EBI-918669,EBI-1053363From a different organism.

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

IntActiQ63615. 3 interactions.
STRINGi10116.ENSRNOP00000059816.

Structurei

3D structure databases

ProteinModelPortaliQ63615.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.Curated

Phylogenomic databases

eggNOGiKOG1302. Eukaryota.
COG5158. LUCA.
HOVERGENiHBG054244.
InParanoidiQ63615.
PhylomeDBiQ63615.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
SUPFAMiSSF56815. SSF56815. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63615-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAHLSYGRV NLNVLREAVR RELREFLDKC AGSKAIVWDE YLTGPFGLIA
60 70 80 90 100
QYSLLKEHEV EKMFTLKGSR LPAADVKNII FLVRPRLELM DMIAENVLSE
110 120 130 140 150
DRRGPTRDFH ILFVPRRSLL CEQRLKDLGV LGSFIYREEY SLDLIPFDGD
160 170 180 190 200
LLSMESESAF KECYLEGDQT SLYHAAKGLM TLQALYGTIP QIFGKGECAR
210 220 230 240 250
QVANMMVRMK REFTGSQNSV FPVFDNLLLL DRNVDLLTPL ASQLTYEGLI
260 270 280 290 300
DEIYGIQNSY VKLPPEKFAP KKQGGGGGKD LPTEAKKLQL NSAEELYAEI
310 320 330 340 350
RDKNFNAVGS VLSKKAKIIS AAFEERHNAK TVGEIKQFVS QLPHMQAARG
360 370 380 390 400
SLANHTSIAE LIKDVTTSED FFDKLTVEQE FMSGIDTDKV NNYIEDCIAQ
410 420 430 440 450
KHPLIKVLRL VCLQSMCNSG LKQKVLDYYK REILQTYGYE HILTLNNLEK
460 470 480 490 500
AGLLKAQTGG RNNYPTIRKT LRLWMDDVNE QNPTDISYVY SGYAPLSVRL
510 520 530 540 550
AQLLSRPGWR SIEEVLRILP GPHFEERQPL PTGVQKKRQP GENRVTLVFF
560 570 580 590
LGGVTFAEIA ALRFLSQLED GGTEYVIATT KLINGSSWLE ALMEKPF
Length:597
Mass (Da):67,514
Last modified:November 1, 1996 - v1
Checksum:i455A3362D56E5188
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35244 mRNA. Translation: AAC52985.1.
PIRiJC5720.
UniGeneiRn.217659.

Genome annotation databases

UCSCiRGD:620643. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35244 mRNA. Translation: AAC52985.1.
PIRiJC5720.
UniGeneiRn.217659.

3D structure databases

ProteinModelPortaliQ63615.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63615. 3 interactions.
STRINGi10116.ENSRNOP00000059816.

PTM databases

PhosphoSiteiQ63615.

Proteomic databases

PaxDbiQ63615.
PRIDEiQ63615.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620643. rat.

Organism-specific databases

RGDi620643. Vps33a.

Phylogenomic databases

eggNOGiKOG1302. Eukaryota.
COG5158. LUCA.
HOVERGENiHBG054244.
InParanoidiQ63615.
PhylomeDBiQ63615.

Miscellaneous databases

PROiQ63615.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 1 hit.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
SUPFAMiSSF56815. SSF56815. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVP33A_RAT
AccessioniPrimary (citable) accession number: Q63615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.