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Protein

Tropomyosin alpha-3 chain

Gene

Tpm3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

GO - Biological processi

  • brain development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-RNO-390522. Striated Muscle Contraction.
R-RNO-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin alpha-3 chain
Alternative name(s):
Gamma-tropomyosin
Tropomyosin-3
Tropomyosin-5
Gene namesi
Name:Tpm3
Synonyms:Tpm-5, Tpm5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi621546. Tpm3.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: UniProtKB
  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 248247Tropomyosin alpha-3 chainPRO_0000289259Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ63610.
PRIDEiQ63610.

PTM databases

iPTMnetiQ63610.

Expressioni

Gene expression databases

GenevisibleiQ63610. RN.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain. Binds to TMOD1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi250770. 3 interactions.
IntActiQ63610. 2 interactions.
STRINGi10116.ENSRNOP00000023567.

Structurei

3D structure databases

ProteinModelPortaliQ63610.
SMRiQ63610. Positions 9-248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili4 – 240237Sequence analysisAdd
BLAST

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1003. Eukaryota.
ENOG410XR5K. LUCA.
GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231522.
HOVERGENiHBG107404.
InParanoidiQ63610.
KOiK09290.
OMAiDESERWM.
OrthoDBiEOG7673C8.
PhylomeDBiQ63610.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63610-1) [UniParc]FASTAAdd to basket

Also known as: Tpm3_v1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGSTTIEAV KRKIQVLQQQ ADDAEERAER LQREVEGERR AREQAEAEVA
60 70 80 90 100
SLNRRIQLVE EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRALK
110 120 130 140 150
DEEKMELQEI QLKEAKHIAE EADRKYEEVA RKLVIIEGDL ERTEERAELA
160 170 180 190 200
ESRCREMDEQ IRLMDQNLKC LSAAEEKYSQ KEDKYEEEIK ILTDKLKEAE
210 220 230 240
TRAEFAERSV AKLEKTIDDL EDKLKCTKEE HLCTQRMLDQ TLLDLNEM
Length:248
Mass (Da):29,007
Last modified:May 29, 2007 - v2
Checksum:iE3E49312A710A068
GO
Isoform 2 (identifier: Q63610-2) [UniParc]FASTAAdd to basket

Also known as: Tpm3_v3

The sequence of this isoform differs from the canonical sequence as follows:
     153-175: RCREMDEQIRLMDQNLKCLSAAE → KCSELEEELKNVTNNLKSLEAQA
     223-248: KLKCTKEEHLCTQRMLDQTLLDLNEM → ELYAQKLKYKAISDELDHALNDMTSI

Show »
Length:248
Mass (Da):28,720
Checksum:iA05D4E189397CAAB
GO
Isoform 3 (identifier: Q63610-3) [UniParc]FASTAAdd to basket

Also known as: Tpm3_v2

The sequence of this isoform differs from the canonical sequence as follows:
     153-175: RCREMDEQIRLMDQNLKCLSAAE → KCSELEEELKNVTNNLKSLEAQA
     222-248: DKLKCTKEEHLCTQRMLDQTLLDLNEM → ERLYSQLERNRLLSNELKLTLHGLCD

Show »
Length:247
Mass (Da):28,709
Checksum:i839796CD1020E962
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831A → V in CAA51382 (PubMed:9222299).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei153 – 17523RCREM…LSAAE → KCSELEEELKNVTNNLKSLE AQA in isoform 2 and isoform 3. 1 PublicationVSP_025995Add
BLAST
Alternative sequencei222 – 24827DKLKC…DLNEM → ERLYSQLERNRLLSNELKLT LHGLCD in isoform 3. 1 PublicationVSP_025996Add
BLAST
Alternative sequencei223 – 24826KLKCT…DLNEM → ELYAQKLKYKAISDELDHAL NDMTSI in isoform 2. 1 PublicationVSP_025997Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24775 mRNA. Translation: AAA21721.1.
L24776 mRNA. Translation: AAA42263.1.
L24777 mRNA. Translation: AAA42264.1.
X72859 mRNA. Translation: CAA51382.1.
PIRiI53784.
I67849.
I67850.
S34124.
RefSeqiNP_001288215.1. NM_001301286.1. [Q63610-2]
NP_476556.2. NM_057208.2. [Q63610-3]
NP_775134.1. NM_173111.1.
UniGeneiRn.37575.

Genome annotation databases

EnsembliENSRNOT00000023567; ENSRNOP00000023567; ENSRNOG00000017441. [Q63610-1]
ENSRNOT00000023605; ENSRNOP00000023605; ENSRNOG00000017441. [Q63610-3]
GeneIDi117557.
KEGGirno:117557.
UCSCiRGD:621546. rat. [Q63610-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24775 mRNA. Translation: AAA21721.1.
L24776 mRNA. Translation: AAA42263.1.
L24777 mRNA. Translation: AAA42264.1.
X72859 mRNA. Translation: CAA51382.1.
PIRiI53784.
I67849.
I67850.
S34124.
RefSeqiNP_001288215.1. NM_001301286.1. [Q63610-2]
NP_476556.2. NM_057208.2. [Q63610-3]
NP_775134.1. NM_173111.1.
UniGeneiRn.37575.

3D structure databases

ProteinModelPortaliQ63610.
SMRiQ63610. Positions 9-248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250770. 3 interactions.
IntActiQ63610. 2 interactions.
STRINGi10116.ENSRNOP00000023567.

PTM databases

iPTMnetiQ63610.

Proteomic databases

PaxDbiQ63610.
PRIDEiQ63610.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023567; ENSRNOP00000023567; ENSRNOG00000017441. [Q63610-1]
ENSRNOT00000023605; ENSRNOP00000023605; ENSRNOG00000017441. [Q63610-3]
GeneIDi117557.
KEGGirno:117557.
UCSCiRGD:621546. rat. [Q63610-1]

Organism-specific databases

CTDi7170.
RGDi621546. Tpm3.

Phylogenomic databases

eggNOGiKOG1003. Eukaryota.
ENOG410XR5K. LUCA.
GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231522.
HOVERGENiHBG107404.
InParanoidiQ63610.
KOiK09290.
OMAiDESERWM.
OrthoDBiEOG7673C8.
PhylomeDBiQ63610.

Enzyme and pathway databases

ReactomeiR-RNO-390522. Striated Muscle Contraction.
R-RNO-445355. Smooth Muscle Contraction.

Miscellaneous databases

NextBioi620413.
PROiQ63610.

Gene expression databases

GenevisibleiQ63610. RN.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of novel alternatively spliced isoforms of the tropomyosin-encoding gene, TMnm, in the rat cochlea."
    Beisel K.W., Kennedy J.E.
    Gene 143:251-256(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: Sprague-Dawley.
    Tissue: Cochlea.
  2. "Transformation-related expression of a low-molecular-mass tropomyosin isoform TM5/TM30nm in transformed rat fibroblastic cell lines."
    Miyado K., Sato M., Taniguchi S.
    J. Cancer Res. Clin. Oncol. 123:331-336(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
  3. Bienvenut W.V., von Kriegsheim A., Kolch W.
    Submitted (JUN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-27 AND 41-65, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fibroblast.
  4. Lubec G., Chen W.-Q., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 13-27; 55-65; 105-113; 132-142; 182-190 AND 216-225, PARTIAL PROTEIN SEQUENCE (ISOFORMS 2 AND 3), IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry nameiTPM3_RAT
AccessioniPrimary (citable) accession number: Q63610
Secondary accession number(s): Q63599, Q63600, Q63601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: May 11, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.