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Reviewed, UniProtKB/Swiss-Prot Q63604 (NTRK2_RAT)

Last modified November 25, 2008. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    BDNF/NT-3 growth factors receptor
    EC=2.7.10.1
Alternative name(s):
    Neurotrophic tyrosine kinase receptor type 2
    TrkB tyrosine kinase
    GP145-TrkB/GP95-TrkB
      Short name=Trk-B
Gene names
Name: Ntrk2
Synonyms: Trkb
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length821 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures By similarity. Binds SH2B2. Interacts with SQSTM1 and ARMS.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

The different forms are differentially expressed in various cell types. Isoform T2 is primarily expressed in neurons.

Post-translational modification

Ligand-mediated auto-phosphorylation.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 2 LRR (leucine-rich) repeats.

Contains 1 protein kinase domain.

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Notes: Additional isoforms seem to exist.
Isoform GP145-TrkB (identifier: Q63604-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform T1 (identifier: Q63604-2)

Also known as: GP95-TrkB;

The sequence of this isoform differs from the canonical sequence as follows:
     466-476: PASVISNDDDS → FVLFHKIPLDG
     477-821: Missing.
Isoform T2 (identifier: Q63604-3)

The sequence of this isoform differs from the canonical sequence as follows:
     466-474: PASVISNDD → KQKCAYFAS
     475-821: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 By similarity
Chain32 – 821790BDNF/NT-3 growth factors receptor
PRO_0000016729

Regions

Topological domain32 – 429398Extracellular Potential
Transmembrane430 – 45324 Potential
Topological domain454 – 821368Cytoplasmic Potential
Repeat72 – 9322LRR 1
Repeat96 – 11722LRR 2
Domain197 – 28286Ig-like C2-type 1
Domain295 – 36571Ig-like C2-type 2
Domain537 – 806270Protein kinase
Nucleotide binding543 – 5519ATP By similarity

Sites

Active site6751Proton acceptor By similarity
Binding site5711ATP By similarity
Site5151Interaction with SHC1 By similarity
Site8161Interaction with PLC-gamma-1 By similarity

Amino acid modifications

Modified residue5151Phosphotyrosine; by autocatalysis By similarity
Modified residue7011Phosphotyrosine; by autocatalysis
Modified residue7051Phosphotyrosine; by autocatalysis
Modified residue7061Phosphotyrosine; by autocatalysis
Modified residue8161Phosphotyrosine; by autocatalysis
Glycosylation671N-linked (GlcNAc...) Potential
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1211N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation2051N-linked (GlcNAc...) Potential
Glycosylation2411N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential
Glycosylation2801N-linked (GlcNAc...) Potential
Glycosylation3251N-linked (GlcNAc...) Potential
Glycosylation3381N-linked (GlcNAc...) Potential
Glycosylation4111N-linked (GlcNAc...) Potential
Disulfide bond32 ↔ 38 By similarity
Disulfide bond36 ↔ 45 By similarity
Disulfide bond152 ↔ 176 By similarity
Disulfide bond154 ↔ 194 By similarity
Disulfide bond218 ↔ 266 By similarity
Disulfide bond302 ↔ 345 By similarity

Natural variations

Alternative sequence466 – 47611PASVISNDDDS → FVLFHKIPLDG in isoform T1.
VSP_002910
Alternative sequence466 – 4749PASVISNDD → KQKCAYFAS in isoform T2.
VSP_002912
Alternative sequence475 – 821347Missing in isoform T2.
VSP_002913
Alternative sequence477 – 821345Missing in isoform T1.
VSP_002911

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Sequences

Sequence LengthMass (Da)Tools
Isoform GP145-TrkB [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0DDACDA212CDAA0E

FASTA82192,186
        10         20         30         40         50         60 
MSPWPRWHGP AMARLWGLCL LVLGFWRASL ACPMSCKCST TRIWCTEPSP GIVAFPRLEP 

        70         80         90        100        110        120 
NSIDPENITE ILIANQKRLE IINEDDVEAY VGLKNLTIVD SGLKFVAYKA FLKNGNLRHI 

       130        140        150        160        170        180 
NFTRNKLTSL SRRHFRHLDL SDLILTGNPF TCSCDIMWLK TLQETKSSPD TQDLYCLNES 

       190        200        210        220        230        240 
SKNTPLANLQ IPNCGLPSAR LAAPNLTVEE GKSVTISCSV GGDPLPTLYW DVGNLVSKHM 

       250        260        270        280        290        300 
NETSHTQGSL RITNISSDDS GKQISCVAEN LVGEDQDSVN LTVHFAPTIT FLESPTSDHH 

       310        320        330        340        350        360 
WCIPFTVRGN PKPALQWFYN GAILNESKYI CTKIHVTNHT EYHGCLQLDN PTHMNNGDYT 

       370        380        390        400        410        420 
LMAKNEYGKD ERQISAHFMG RPGVDYETNP NYPEVLYEDW TTPTDIGDTT NKSNEIPSTD 

       430        440        450        460        470        480 
VADQTNREHL SVYAVVVIAS VVGFCLLVML LLLKLARHSK FGMKGPASVI SNDDDSASPL 

       490        500        510        520        530        540 
HHISNGSNTP SSSEGGPDAV IIGMTKIPVI ENPQYFGITN SQLKPDTFVQ HIKRHNIVLK 

       550        560        570        580        590        600 
RELGEGAFGK VFLAECYNLC PEQDKILVAV KTLKDASDNA RKDFHREAEL LTNLQHEHIV 

       610        620        630        640        650        660 
KFYGVCVEGD PLIMVFEYMK HGDLNKFLRA HGPDAVLMAE GNPPTELTQS QMLHIAQQIA 

       670        680        690        700        710        720 
AGMVYLASQH FVHRDLATRN CLVGENLLVK IGDFGMSRDV YSTDYYRVGG HTMLPIRWMP 

       730        740        750        760        770        780 
PESIMYRKFT TESDVWSLGV VLWEIFTYGK QPWYQLSNNE VIECITQGRV LQRPRTCPQE 

       790        800        810        820 
VYELMLGCWQ REPHTRKNIK NIHTLLQNLA KASPVYLDIL G

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Isoform T1 (GP95-TrkB) [UniParc].

Checksum: 79AFCD9CA0AB13A8
Show »

47653,212
Isoform T2 [UniParc].

Checksum: 2A6F19237F9F692A
Show »

47452,971

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References

[1]"trkB, a neural receptor protein-tyrosine kinase: evidence for a full-length and two truncated receptors."
Middlemas D.S., Lindberg R.A., Hunter T.
Mol. Cell. Biol. 11:143-153(1991) [PubMed: 1846020] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Tissue: Cerebellum.
[2]"Identification of TrkB autophosphorylation sites and evidence that phospholipase C-gamma 1 is a substrate of the TrkB receptor."
Middlemas D.S., Meisenhelder J., Hunter T.
J. Biol. Chem. 269:5458-5466(1994) [PubMed: 8106527] [Abstract]
Cited for: PHOSPHORYLATION AT TYR-701; TYR-705; TYR-706 AND TYR-816.
[3]"Identification and characterization of novel substrates of Trk receptors in developing neurons."
Qian X., Riccio A., Zhang Y., Ginty D.D.
Neuron 21:1017-1029(1998) [PubMed: 9856458] [Abstract]
Cited for: INTERACTION WITH SH2B2.
[4]"Association of the atypical protein kinase C-interacting protein p62/ZIP with nerve growth factor receptor TrkA regulates receptor trafficking and Erk5 signaling."
Geetha T., Wooten M.W.
J. Biol. Chem. 278:4730-4739(2003) [PubMed: 12471037] [Abstract]
Cited for: INTERACTION WITH SQSTM1.
[5]"A unique pathway for sustained neurotrophin signaling through an ankyrin-rich membrane-spanning protein."
Arevalo J.C., Yano H., Teng K.K., Chao M.V.
EMBO J. 23:2358-2368(2004) [PubMed: 15167895] [Abstract]
Cited for: INTERACTION WITH ARMS.

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Cross-references

Sequence databases

M55291 mRNA. Translation: AAA42279.1.
M55292 mRNA. Translation: AAA42280.1.
M55293 mRNA. Translation: AAA42281.1.
PIRA39667.
B39667.
C39667.
RefSeqNP_036863.1.
UniGeneRn.11246

3D structure databases

HSSPHSSP built from PDB template 1WWB based on UniProtKB Q16620.
SMRQ63604. Positions 283-385.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:5717N.

PTM databases

PhosphoSiteQ63604.

Genome annotation databases

EnsemblENSRNOG00000018839. Rattus norvegicus. [Contig view]
GeneID25054.
KEGGrno:25054.

Organism-specific databases

RGD3213. Ntrk2.

Phylogenomic databases

HOVERGENQ63604.

Gene expression databases

ArrayExpressQ63604.
GermOnlineENSRNOG00000018839. Rattus norvegicus.

Family and domain databases

InterProIPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR000483. LRR_C.
IPR000372. LRR_cys_N.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_bd_CS.
IPR002011. Recept_tyr_kinase-II_CS.
IPR001245. Tyr_pkinase.
IPR008266. Tyr_pkinase_AS.
[Graphical view]
Gene3DG3DSA:2.60.40.10. Ig-like_fold. 2 hits.
PfamPF07679. I-set. 1 hit.
PF01462. LRRNT. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00408. IGc2. 1 hit.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00239. RECEPTOR_TYR_KIN_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio605258.

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Entry information

Entry nameNTRK2_RAT
AccessionPrimary (citable) accession number: Q63604
Secondary accession number(s): Q63605, Q63606
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents