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Protein

Dual specificity testis-specific protein kinase 1

Gene

Tesk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Probably plays a central role at and after the meiotic phase of spermatogenesis.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Activated by autophosphorylation on Ser-215. Kinase activity is inhibited by SPRED1.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81ATPPROSITE-ProRule annotation1
Active sitei170Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 66ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity testis-specific protein kinase 1 (EC:2.7.12.1)
Alternative name(s):
Testicular protein kinase 1
Gene namesi
Name:Tesk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi62059. Tesk1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170D → A: Loss of kinase and autophosphorylating activity. 1
Mutagenesisi201Y → A: No effect on autophosphorylation. 1 Publication1
Mutagenesisi215S → A: Loss of autophosphorylation site, loss of kinase activity. 1 Publication1
Mutagenesisi215S → E: Loss of autophosphorylation site, no effect on kinase activity. 1 Publication1
Mutagenesisi217Y → A: No effect on autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867481 – 628Dual specificity testis-specific protein kinase 1Add BLAST628

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei215Phosphoserine; by autocatalysis1 Publication1
Modified residuei338Omega-N-methylarginineBy similarity1

Post-translational modificationi

Autophosphorylated on serine and tyrosine residues.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ63572.
PRIDEiQ63572.

PTM databases

iPTMnetiQ63572.
PhosphoSitePlusiQ63572.

Expressioni

Tissue specificityi

Highly expressed in testicular germ cells. Expressed at low levels in brain, lung, heart, liver and kidney.1 Publication

Gene expression databases

BgeeiENSRNOG00000017532.
GenevisibleiQ63572. RN.

Interactioni

Subunit structurei

Interacts with SPRY4 (By similarity). Interacts with TAOK2; leading to inhibit TAOK2 activity. Interacts with SPRED1; leading to inhibit activity.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023694.

Structurei

3D structure databases

ProteinModelPortaliQ63572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 310Protein kinasePROSITE-ProRule annotationAdd BLAST259

Domaini

The extracatalytic C-terminal part is highly rich in proline residues.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IF1S. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00530000063025.
HOGENOMiHOG000231415.
HOVERGENiHBG058204.
InParanoidiQ63572.
KOiK08841.
OMAiKMECEGS.
OrthoDBiEOG091G0HLL.
PhylomeDBiQ63572.
TreeFamiTF318014.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR015782. TESK1.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PANTHERiPTHR23257:SF383. PTHR23257:SF383. 1 hit.
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGERPPLRG PGPGETPVEG PGGAGGGPGR GRPSSYRALR SAVSSLARVD
60 70 80 90 100
DFDCAEKIGA GFFSEVYKVR HRQSGQVMVL KMNKLPSNRS NTLREVQLMN
110 120 130 140 150
RLRHPNILRF MGVCVHQGQL HALTEYMNGG TLEQLLSSPE PLSWPVRLHL
160 170 180 190 200
ALDIAQGLRY LHAKGVFHRD LTSKNCLVRR EDGGFTAVVG DFGLAEKIPV
210 220 230 240 250
YREGARKEPL AVVGSPYWMA PEVLRGELYD EKADVFAFGI VLCELIARVP
260 270 280 290 300
ADPDYLPRTE DFGLDVPAFR TLVGNDCPLP FLLLAIHCCS MEPSARAPFT
310 320 330 340 350
EITQHLEQIL EQLPEPTPLA KMPLAKAPLT YNQGSVPRGG PSATLPRSDP
360 370 380 390 400
RLSRSRSDLF LPPSPESPPS WGDNLTRVNP FSLREDLRGG KIKLLDTPCK
410 420 430 440 450
PATPLPLVPP SPLTSTQLPL VASPESLVQP ETPVRRCRSL PSSPELPRRM
460 470 480 490 500
ETALPGPGPS PVGPSTEERM DCEGSSPEPE PPGPAPQLPL AVATDNFIST
510 520 530 540 550
CSSASQPWSA RPGPSLNNNP PAVVVNSPQG WAREPWNRAQ HSLPRAAALE
560 570 580 590 600
RTEPSPPPSA PREQEEGLPC PGCCLSPFSF GFLSMCPRPT PAVARYRNLN
610 620
CEAGSLLCHR GHHAKPPTPS LQLPGARS
Length:628
Mass (Da):67,988
Last modified:November 1, 1996 - v1
Checksum:iF05F67FBD934B9AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50864 mRNA. Translation: BAA09460.1.
BC081773 mRNA. Translation: AAH81773.1.
RefSeqiNP_113766.1. NM_031578.1.
UniGeneiRn.7006.

Genome annotation databases

EnsembliENSRNOT00000080909; ENSRNOP00000071965; ENSRNOG00000053729.
GeneIDi29460.
KEGGirno:29460.
UCSCiRGD:62059. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50864 mRNA. Translation: BAA09460.1.
BC081773 mRNA. Translation: AAH81773.1.
RefSeqiNP_113766.1. NM_031578.1.
UniGeneiRn.7006.

3D structure databases

ProteinModelPortaliQ63572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023694.

PTM databases

iPTMnetiQ63572.
PhosphoSitePlusiQ63572.

Proteomic databases

PaxDbiQ63572.
PRIDEiQ63572.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000080909; ENSRNOP00000071965; ENSRNOG00000053729.
GeneIDi29460.
KEGGirno:29460.
UCSCiRGD:62059. rat.

Organism-specific databases

CTDi7016.
RGDi62059. Tesk1.

Phylogenomic databases

eggNOGiENOG410IF1S. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00530000063025.
HOGENOMiHOG000231415.
HOVERGENiHBG058204.
InParanoidiQ63572.
KOiK08841.
OMAiKMECEGS.
OrthoDBiEOG091G0HLL.
PhylomeDBiQ63572.
TreeFamiTF318014.

Enzyme and pathway databases

BRENDAi2.7.10.2. 5301.

Miscellaneous databases

PROiQ63572.

Gene expression databases

BgeeiENSRNOG00000017532.
GenevisibleiQ63572. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR015782. TESK1.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PANTHERiPTHR23257:SF383. PTHR23257:SF383. 1 hit.
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTESK1_RAT
AccessioniPrimary (citable) accession number: Q63572
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.