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Q63564

- SV2B_RAT

UniProt

Q63564 - SV2B_RAT

Protein

Synaptic vesicle glycoprotein 2B

Gene

Sv2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Probably plays a role in the control of regulated secretion in neural and endocrine cells.By similarity
    Receptor for the botulinium neurotoxin type A/BOTA.1 Publication

    GO - Molecular functioni

    1. transmembrane transporter activity Source: InterPro

    GO - Biological processi

    1. neurotransmitter uptake Source: RGD

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptic vesicle glycoprotein 2B
    Short name:
    Synaptic vesicle protein 2B
    Gene namesi
    Name:Sv2b
    Synonyms:Sv2bb
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619716. Sv2b.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Multi-pass membrane protein. Cytoplasmic vesiclesecretory vesicleacrosome
    Note: Associated with synaptic-like microvesicles but not with insulin-containing vesicles in insulin-secreting cells of the pancreas. Localizes to microvesicles in the pinealocytes. Localizes to the acrosome in spermatids.

    GO - Cellular componenti

    1. acrosomal vesicle Source: UniProtKB-SubCell
    2. cell junction Source: UniProtKB-KW
    3. integral component of membrane Source: UniProtKB-KW
    4. membrane Source: RGD
    5. synaptic vesicle Source: RGD
    6. synaptic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 683683Synaptic vesicle glycoprotein 2BPRO_0000239770Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei423 – 4231PhosphotyrosineBy similarity
    Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi516 – 5161N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication
    The N-terminal cytoplasmic domain is phosphorylated by CK1.1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ63564.
    PRIDEiQ63564.

    PTM databases

    PhosphoSiteiQ63564.

    Expressioni

    Tissue specificityi

    Widely expressed throughout the brain. Specifically expressed by pinealocytes in the pineal gland. Also detected in testis (at protein level). Specifically expressed in neural tissues. Expressed in the spinal cord and in all brain regions with a stronger expression in hippocampus and cortex.4 Publications

    Inductioni

    By amyloid beta peptide.1 Publication

    Gene expression databases

    GenevestigatoriQ63564.

    Interactioni

    Subunit structurei

    Interacts with SYT1 in a calcium-independent manner. Forms a complex with SYT1, syntaxin-1 and SNAP25 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi250769. 1 interaction.
    IntActiQ63564. 3 interactions.
    STRINGi10116.ENSRNOP00000015084.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63564.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 110110CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini132 – 14817ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini170 – 18213CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini204 – 2052ExtracellularSequence Analysis
    Topological domaini227 – 23711CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini259 – 27719ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini299 – 39092CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini412 – 535124ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini557 – 5659CytoplasmicSequence Analysis
    Topological domaini587 – 5926ExtracellularSequence Analysis
    Topological domaini614 – 62613CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini650 – 6534ExtracellularSequence Analysis
    Topological domaini673 – 68311CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei111 – 13121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei149 – 16921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei183 – 20321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei206 – 22621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei238 – 25821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei278 – 29821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei391 – 41121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei536 – 55621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei566 – 58621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei593 – 61321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei627 – 64923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei654 – 67219HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni486 – 52338BOTA-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the major facilitator superfamily.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    HOGENOMiHOG000065727.
    HOVERGENiHBG053967.
    InParanoidiQ63564.
    KOiK06258.
    PhylomeDBiQ63564.

    Family and domain databases

    InterProiIPR001646. 5peptide_repeat.
    IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR005829. Sugar_transporter_CS.
    IPR022308. SV2.
    [Graphical view]
    PANTHERiPTHR24065. PTHR24065. 1 hit.
    PfamiPF07690. MFS_1. 1 hit.
    PF13599. Pentapeptide_4. 1 hit.
    PF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 3 hits.
    TIGRFAMsiTIGR01299. synapt_SV2. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q63564-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDYRYRDNY EGYAPNDGYY RGNEQNPEED AQSDVTEGHD EEDEIYEGEY    50
    QGIPHPDDVK SKQTKMAPSR ADGLRGQADL MAERMEDEEQ LAHQYETIID 100
    ECGHGRFQWT LFFVLVLALM ADGVEVFVVS FALPSAEKDM CLSSSKKGML 150
    GLIVYLGMMA GAFILGGLAD KLGRKKVLSM SLAINASFAS LSSFVQGYGA 200
    FLFCRLISGI GIGGSLPIVF AYFSEFLSRE KRGEHLSWLG IFWMTGGIYA 250
    SAMAWSIIPH YGWGFSMGTN YHFHSWRVFV IVCALPATVS MVALKFMPES 300
    PRFLLEMGKH DEAWMILKQV HDTNMRAKGT PEKVFTVSHI KTPKQMDEFI 350
    EIQSSTGTWY QRWLVRFMTI FKQVWDNALY CVMGPYRMNT LILAVVWFTM 400
    ALSYYGLTVW FPDMIRYFQD EEYKSKMKVF FGEHVHGATI NFTMENQIHQ 450
    HGKLVNDKFI KMYFKHVLFE DTFFDKCYFE DVTSTDTYFK NCTIESTTFY 500
    NTDLYKHKFI DCRFINSTFL EQKEGCHMDF EEDNDFLIYL VSFLGSLSVL 550
    PGNIISALLM DRIGRLKMIG GSMLISAVCC FFLFFGNSES AMIGWQCLFC 600
    GTSIAAWNAL DVITVELYPT NQRATAFGIL NGLCKLGAIL GNTIFASFVG 650
    ITKVVPILLA AASLVGGGLV ALRLPETREQ VLM 683
    Length:683
    Mass (Da):77,502
    Last modified:November 1, 1996 - v1
    Checksum:iBF862EFD4B99EA7B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10362 mRNA. Translation: AAA42189.1.
    AF372834 mRNA. Translation: AAL47714.1.
    PIRiS34961.
    RefSeqiNP_476555.1. NM_057207.3.
    UniGeneiRn.58137.

    Genome annotation databases

    GeneIDi117556.
    KEGGirno:117556.
    UCSCiRGD:619716. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10362 mRNA. Translation: AAA42189.1 .
    AF372834 mRNA. Translation: AAL47714.1 .
    PIRi S34961.
    RefSeqi NP_476555.1. NM_057207.3.
    UniGenei Rn.58137.

    3D structure databases

    ProteinModelPortali Q63564.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250769. 1 interaction.
    IntActi Q63564. 3 interactions.
    STRINGi 10116.ENSRNOP00000015084.

    PTM databases

    PhosphoSitei Q63564.

    Proteomic databases

    PaxDbi Q63564.
    PRIDEi Q63564.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 117556.
    KEGGi rno:117556.
    UCSCi RGD:619716. rat.

    Organism-specific databases

    CTDi 9899.
    RGDi 619716. Sv2b.

    Phylogenomic databases

    eggNOGi COG0477.
    HOGENOMi HOG000065727.
    HOVERGENi HBG053967.
    InParanoidi Q63564.
    KOi K06258.
    PhylomeDBi Q63564.

    Miscellaneous databases

    NextBioi 620409.

    Gene expression databases

    Genevestigatori Q63564.

    Family and domain databases

    InterProi IPR001646. 5peptide_repeat.
    IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR005829. Sugar_transporter_CS.
    IPR022308. SV2.
    [Graphical view ]
    PANTHERi PTHR24065. PTHR24065. 1 hit.
    Pfami PF07690. MFS_1. 1 hit.
    PF13599. Pentapeptide_4. 1 hit.
    PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 3 hits.
    TIGRFAMsi TIGR01299. synapt_SV2. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. "Identification of a new synaptic vesicle protein 2B mRNA transcript which is up-regulated in neurons by amyloid beta peptide fragment (1-42)."
      Heese K., Nagai Y., Sawada T.
      Biochem. Biophys. Res. Commun. 289:924-928(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    3. "Synaptic vesicle protein SV2B, but not SV2A, is predominantly expressed and associated with microvesicles in rat pinealocytes."
      Hayashi M., Yamamoto A., Yatsushiro S., Yamada H., Futai M., Yamaguchi A., Moriyama Y.
      J. Neurochem. 71:356-365(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "SVOP, an evolutionarily conserved synaptic vesicle protein, suggests novel transport functions of synaptic vesicles."
      Janz R., Hofmann K., Suedhof T.C.
      J. Neurosci. 18:9269-9281(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION.
    5. "SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family."
      Janz R., Suedhof T.C.
      Neuroscience 94:1279-1290(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Phosphorylation of synaptic vesicle protein 2 modulates binding to synaptotagmin."
      Pyle R.A., Schivell A.E., Hidaka H., Bajjalieh S.M.
      J. Biol. Chem. 275:17195-17200(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY CK1.
    7. "Brain synaptic junctional proteins at the acrosome of rat testicular germ cells."
      Redecker P., Kreutz M.R., Bockmann J., Gundelfinger E.D., Boeckers T.M.
      J. Histochem. Cytochem. 51:809-819(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    8. "SV2A and SV2C are not vesicular Ca2+ transporters but control glucose-evoked granule recruitment."
      Iezzi M., Theander S., Janz R., Loze C., Wollheim C.B.
      J. Cell Sci. 118:5647-5660(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. Cited for: INTERACTION WITH SYT1.
    10. "SV2 is the protein receptor for botulinum neurotoxin A."
      Dong M., Yeh F., Tepp W.H., Dean C., Johnson E.A., Janz R., Chapman E.R.
      Science 312:592-596(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A BOTA RECEPTOR.

    Entry informationi

    Entry nameiSV2B_RAT
    AccessioniPrimary (citable) accession number: Q63564
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3