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Q63564 (SV2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptic vesicle glycoprotein 2B

Short name=Synaptic vesicle protein 2B
Gene names
Name:Sv2b
Synonyms:Sv2bb
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length683 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably plays a role in the control of regulated secretion in neural and endocrine cells By similarity. Ref.10

Receptor for the botulinium neurotoxin type A/BOTA. Ref.10

Subunit structure

Interacts with SYT1 in a calcium-independent manner. Forms a complex with SYT1, syntaxin-1 and SNAP25 By similarity. Ref.9

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Multi-pass membrane protein. Cytoplasmic vesiclesecretory vesicleacrosome. Note: Associated with synaptic-like microvesicles but not with insulin-containing vesicles in insulin-secreting cells of the pancreas. Localizes to microvesicles in the pinealocytes. Localizes to the acrosome in spermatids. Ref.1 Ref.7 Ref.8

Tissue specificity

Widely expressed throughout the brain. Specifically expressed by pinealocytes in the pineal gland. Also detected in testis (at protein level). Specifically expressed in neural tissues. Expressed in the spinal cord and in all brain regions with a stronger expression in hippocampus and cortex. Ref.1 Ref.3 Ref.5 Ref.7

Induction

By amyloid beta peptide. Ref.2

Post-translational modification

N-glycosylated. Ref.4

The N-terminal cytoplasmic domain is phosphorylated by CK1. Ref.6

Sequence similarities

Belongs to the major facilitator superfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 683683Synaptic vesicle glycoprotein 2B
PRO_0000239770

Regions

Topological domain1 – 110110Cytoplasmic Potential
Transmembrane111 – 13121Helical; Potential
Topological domain132 – 14817Extracellular Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 18213Cytoplasmic Potential
Transmembrane183 – 20321Helical; Potential
Topological domain204 – 2052Extracellular Potential
Transmembrane206 – 22621Helical; Potential
Topological domain227 – 23711Cytoplasmic Potential
Transmembrane238 – 25821Helical; Potential
Topological domain259 – 27719Extracellular Potential
Transmembrane278 – 29821Helical; Potential
Topological domain299 – 39092Cytoplasmic Potential
Transmembrane391 – 41121Helical; Potential
Topological domain412 – 535124Extracellular Potential
Transmembrane536 – 55621Helical; Potential
Topological domain557 – 5659Cytoplasmic Potential
Transmembrane566 – 58621Helical; Potential
Topological domain587 – 5926Extracellular Potential
Transmembrane593 – 61321Helical; Potential
Topological domain614 – 62613Cytoplasmic Potential
Transmembrane627 – 64923Helical; Potential
Topological domain650 – 6534Extracellular Potential
Transmembrane654 – 67219Helical; Potential
Topological domain673 – 68311Cytoplasmic Potential
Region486 – 52338BOTA-binding By similarity

Amino acid modifications

Modified residue4231Phosphotyrosine By similarity
Glycosylation4411N-linked (GlcNAc...) Potential
Glycosylation4911N-linked (GlcNAc...) Potential
Glycosylation5161N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q63564 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BF862EFD4B99EA7B

FASTA68377,502
        10         20         30         40         50         60 
MDDYRYRDNY EGYAPNDGYY RGNEQNPEED AQSDVTEGHD EEDEIYEGEY QGIPHPDDVK 

        70         80         90        100        110        120 
SKQTKMAPSR ADGLRGQADL MAERMEDEEQ LAHQYETIID ECGHGRFQWT LFFVLVLALM 

       130        140        150        160        170        180 
ADGVEVFVVS FALPSAEKDM CLSSSKKGML GLIVYLGMMA GAFILGGLAD KLGRKKVLSM 

       190        200        210        220        230        240 
SLAINASFAS LSSFVQGYGA FLFCRLISGI GIGGSLPIVF AYFSEFLSRE KRGEHLSWLG 

       250        260        270        280        290        300 
IFWMTGGIYA SAMAWSIIPH YGWGFSMGTN YHFHSWRVFV IVCALPATVS MVALKFMPES 

       310        320        330        340        350        360 
PRFLLEMGKH DEAWMILKQV HDTNMRAKGT PEKVFTVSHI KTPKQMDEFI EIQSSTGTWY 

       370        380        390        400        410        420 
QRWLVRFMTI FKQVWDNALY CVMGPYRMNT LILAVVWFTM ALSYYGLTVW FPDMIRYFQD 

       430        440        450        460        470        480 
EEYKSKMKVF FGEHVHGATI NFTMENQIHQ HGKLVNDKFI KMYFKHVLFE DTFFDKCYFE 

       490        500        510        520        530        540 
DVTSTDTYFK NCTIESTTFY NTDLYKHKFI DCRFINSTFL EQKEGCHMDF EEDNDFLIYL 

       550        560        570        580        590        600 
VSFLGSLSVL PGNIISALLM DRIGRLKMIG GSMLISAVCC FFLFFGNSES AMIGWQCLFC 

       610        620        630        640        650        660 
GTSIAAWNAL DVITVELYPT NQRATAFGIL NGLCKLGAIL GNTIFASFVG ITKVVPILLA 

       670        680 
AASLVGGGLV ALRLPETREQ VLM 

« Hide

References

[1]"Brain contains two forms of synaptic vesicle protein 2."
Bajjalieh S.M., Peterson K.E., Linial M., Scheller R.H.
Proc. Natl. Acad. Sci. U.S.A. 90:2150-2154(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Identification of a new synaptic vesicle protein 2B mRNA transcript which is up-regulated in neurons by amyloid beta peptide fragment (1-42)."
Heese K., Nagai Y., Sawada T.
Biochem. Biophys. Res. Commun. 289:924-928(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
[3]"Synaptic vesicle protein SV2B, but not SV2A, is predominantly expressed and associated with microvesicles in rat pinealocytes."
Hayashi M., Yamamoto A., Yatsushiro S., Yamada H., Futai M., Yamaguchi A., Moriyama Y.
J. Neurochem. 71:356-365(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"SVOP, an evolutionarily conserved synaptic vesicle protein, suggests novel transport functions of synaptic vesicles."
Janz R., Hofmann K., Suedhof T.C.
J. Neurosci. 18:9269-9281(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION.
[5]"SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family."
Janz R., Suedhof T.C.
Neuroscience 94:1279-1290(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Phosphorylation of synaptic vesicle protein 2 modulates binding to synaptotagmin."
Pyle R.A., Schivell A.E., Hidaka H., Bajjalieh S.M.
J. Biol. Chem. 275:17195-17200(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CK1.
[7]"Brain synaptic junctional proteins at the acrosome of rat testicular germ cells."
Redecker P., Kreutz M.R., Bockmann J., Gundelfinger E.D., Boeckers T.M.
J. Histochem. Cytochem. 51:809-819(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"SV2A and SV2C are not vesicular Ca2+ transporters but control glucose-evoked granule recruitment."
Iezzi M., Theander S., Janz R., Loze C., Wollheim C.B.
J. Cell Sci. 118:5647-5660(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"SV2A and SV2C contain a unique synaptotagmin-binding site."
Schivell A.E., Mochida S., Kensel-Hammes P., Custer K.L., Bajjalieh S.M.
Mol. Cell. Neurosci. 29:56-64(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SYT1.
[10]"SV2 is the protein receptor for botulinum neurotoxin A."
Dong M., Yeh F., Tepp W.H., Dean C., Johnson E.A., Janz R., Chapman E.R.
Science 312:592-596(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A BOTA RECEPTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10362 mRNA. Translation: AAA42189.1.
AF372834 mRNA. Translation: AAL47714.1.
PIRS34961.
RefSeqNP_476555.1. NM_057207.3.
UniGeneRn.58137.

3D structure databases

ProteinModelPortalQ63564.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250769. 1 interaction.
STRING10116.ENSRNOP00000015084.

PTM databases

PhosphoSiteQ63564.

Proteomic databases

PaxDbQ63564.
PRIDEQ63564.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID117556.
KEGGrno:117556.
UCSCRGD:619716. rat.

Organism-specific databases

CTD9899.
RGD619716. Sv2b.

Phylogenomic databases

eggNOGCOG0477.
HOGENOMHOG000065727.
HOVERGENHBG053967.
InParanoidQ63564.
KOK06258.
PhylomeDBQ63564.

Gene expression databases

GenevestigatorQ63564.

Family and domain databases

InterProIPR001646. 5peptide_repeat.
IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
IPR022308. SV2.
[Graphical view]
PANTHERPTHR24065. PTHR24065. 1 hit.
PfamPF07690. MFS_1. 1 hit.
PF13599. Pentapeptide_4. 1 hit.
PF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 3 hits.
TIGRFAMsTIGR01299. synapt_SV2. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio620409.

Entry information

Entry nameSV2B_RAT
AccessionPrimary (citable) accession number: Q63564
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families