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Q63564

- SV2B_RAT

UniProt

Q63564 - SV2B_RAT

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Protein

Synaptic vesicle glycoprotein 2B

Gene

Sv2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probably plays a role in the control of regulated secretion in neural and endocrine cells.By similarity
Receptor for the botulinium neurotoxin type A/BOTA.1 Publication

GO - Molecular functioni

  1. transmembrane transporter activity Source: InterPro

GO - Biological processi

  1. neurotransmitter uptake Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicle glycoprotein 2B
Short name:
Synaptic vesicle protein 2B
Gene namesi
Name:Sv2b
Synonyms:Sv2bb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619716. Sv2b.

Subcellular locationi

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Multi-pass membrane protein. Cytoplasmic vesiclesecretory vesicleacrosome
Note: Associated with synaptic-like microvesicles but not with insulin-containing vesicles in insulin-secreting cells of the pancreas. Localizes to microvesicles in the pinealocytes. Localizes to the acrosome in spermatids.

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: RGD
  4. synaptic vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 683683Synaptic vesicle glycoprotein 2BPRO_0000239770Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei423 – 4231PhosphotyrosineBy similarity
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi516 – 5161N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.1 Publication
The N-terminal cytoplasmic domain is phosphorylated by CK1.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ63564.
PRIDEiQ63564.

PTM databases

PhosphoSiteiQ63564.

Expressioni

Tissue specificityi

Widely expressed throughout the brain. Specifically expressed by pinealocytes in the pineal gland. Also detected in testis (at protein level). Specifically expressed in neural tissues. Expressed in the spinal cord and in all brain regions with a stronger expression in hippocampus and cortex.4 Publications

Inductioni

By amyloid beta peptide.1 Publication

Gene expression databases

GenevestigatoriQ63564.

Interactioni

Subunit structurei

Interacts with SYT1 in a calcium-independent manner. Forms a complex with SYT1, syntaxin-1 and SNAP25 (By similarity).By similarity

Protein-protein interaction databases

BioGridi250769. 1 interaction.
IntActiQ63564. 3 interactions.
STRINGi10116.ENSRNOP00000015084.

Structurei

3D structure databases

ProteinModelPortaliQ63564.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 110110CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini132 – 14817ExtracellularSequence AnalysisAdd
BLAST
Topological domaini170 – 18213CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini204 – 2052ExtracellularSequence Analysis
Topological domaini227 – 23711CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini259 – 27719ExtracellularSequence AnalysisAdd
BLAST
Topological domaini299 – 39092CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini412 – 535124ExtracellularSequence AnalysisAdd
BLAST
Topological domaini557 – 5659CytoplasmicSequence Analysis
Topological domaini587 – 5926ExtracellularSequence Analysis
Topological domaini614 – 62613CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini650 – 6534ExtracellularSequence Analysis
Topological domaini673 – 68311CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei111 – 13121HelicalSequence AnalysisAdd
BLAST
Transmembranei149 – 16921HelicalSequence AnalysisAdd
BLAST
Transmembranei183 – 20321HelicalSequence AnalysisAdd
BLAST
Transmembranei206 – 22621HelicalSequence AnalysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence AnalysisAdd
BLAST
Transmembranei278 – 29821HelicalSequence AnalysisAdd
BLAST
Transmembranei391 – 41121HelicalSequence AnalysisAdd
BLAST
Transmembranei536 – 55621HelicalSequence AnalysisAdd
BLAST
Transmembranei566 – 58621HelicalSequence AnalysisAdd
BLAST
Transmembranei593 – 61321HelicalSequence AnalysisAdd
BLAST
Transmembranei627 – 64923HelicalSequence AnalysisAdd
BLAST
Transmembranei654 – 67219HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni486 – 52338BOTA-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
HOGENOMiHOG000065727.
HOVERGENiHBG053967.
InParanoidiQ63564.
KOiK06258.
PhylomeDBiQ63564.

Family and domain databases

InterProiIPR001646. 5peptide_repeat.
IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
IPR022308. SV2.
[Graphical view]
PANTHERiPTHR24065. PTHR24065. 1 hit.
PfamiPF07690. MFS_1. 1 hit.
PF13599. Pentapeptide_4. 1 hit.
PF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR01299. synapt_SV2. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63564-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDYRYRDNY EGYAPNDGYY RGNEQNPEED AQSDVTEGHD EEDEIYEGEY
60 70 80 90 100
QGIPHPDDVK SKQTKMAPSR ADGLRGQADL MAERMEDEEQ LAHQYETIID
110 120 130 140 150
ECGHGRFQWT LFFVLVLALM ADGVEVFVVS FALPSAEKDM CLSSSKKGML
160 170 180 190 200
GLIVYLGMMA GAFILGGLAD KLGRKKVLSM SLAINASFAS LSSFVQGYGA
210 220 230 240 250
FLFCRLISGI GIGGSLPIVF AYFSEFLSRE KRGEHLSWLG IFWMTGGIYA
260 270 280 290 300
SAMAWSIIPH YGWGFSMGTN YHFHSWRVFV IVCALPATVS MVALKFMPES
310 320 330 340 350
PRFLLEMGKH DEAWMILKQV HDTNMRAKGT PEKVFTVSHI KTPKQMDEFI
360 370 380 390 400
EIQSSTGTWY QRWLVRFMTI FKQVWDNALY CVMGPYRMNT LILAVVWFTM
410 420 430 440 450
ALSYYGLTVW FPDMIRYFQD EEYKSKMKVF FGEHVHGATI NFTMENQIHQ
460 470 480 490 500
HGKLVNDKFI KMYFKHVLFE DTFFDKCYFE DVTSTDTYFK NCTIESTTFY
510 520 530 540 550
NTDLYKHKFI DCRFINSTFL EQKEGCHMDF EEDNDFLIYL VSFLGSLSVL
560 570 580 590 600
PGNIISALLM DRIGRLKMIG GSMLISAVCC FFLFFGNSES AMIGWQCLFC
610 620 630 640 650
GTSIAAWNAL DVITVELYPT NQRATAFGIL NGLCKLGAIL GNTIFASFVG
660 670 680
ITKVVPILLA AASLVGGGLV ALRLPETREQ VLM
Length:683
Mass (Da):77,502
Last modified:November 1, 1996 - v1
Checksum:iBF862EFD4B99EA7B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10362 mRNA. Translation: AAA42189.1.
AF372834 mRNA. Translation: AAL47714.1.
PIRiS34961.
RefSeqiNP_476555.1. NM_057207.3.
UniGeneiRn.58137.

Genome annotation databases

GeneIDi117556.
KEGGirno:117556.
UCSCiRGD:619716. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10362 mRNA. Translation: AAA42189.1 .
AF372834 mRNA. Translation: AAL47714.1 .
PIRi S34961.
RefSeqi NP_476555.1. NM_057207.3.
UniGenei Rn.58137.

3D structure databases

ProteinModelPortali Q63564.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250769. 1 interaction.
IntActi Q63564. 3 interactions.
STRINGi 10116.ENSRNOP00000015084.

PTM databases

PhosphoSitei Q63564.

Proteomic databases

PaxDbi Q63564.
PRIDEi Q63564.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 117556.
KEGGi rno:117556.
UCSCi RGD:619716. rat.

Organism-specific databases

CTDi 9899.
RGDi 619716. Sv2b.

Phylogenomic databases

eggNOGi COG0477.
HOGENOMi HOG000065727.
HOVERGENi HBG053967.
InParanoidi Q63564.
KOi K06258.
PhylomeDBi Q63564.

Miscellaneous databases

NextBioi 620409.

Gene expression databases

Genevestigatori Q63564.

Family and domain databases

InterProi IPR001646. 5peptide_repeat.
IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
IPR022308. SV2.
[Graphical view ]
PANTHERi PTHR24065. PTHR24065. 1 hit.
Pfami PF07690. MFS_1. 1 hit.
PF13599. Pentapeptide_4. 1 hit.
PF00083. Sugar_tr. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 3 hits.
TIGRFAMsi TIGR01299. synapt_SV2. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Identification of a new synaptic vesicle protein 2B mRNA transcript which is up-regulated in neurons by amyloid beta peptide fragment (1-42)."
    Heese K., Nagai Y., Sawada T.
    Biochem. Biophys. Res. Commun. 289:924-928(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
  3. "Synaptic vesicle protein SV2B, but not SV2A, is predominantly expressed and associated with microvesicles in rat pinealocytes."
    Hayashi M., Yamamoto A., Yatsushiro S., Yamada H., Futai M., Yamaguchi A., Moriyama Y.
    J. Neurochem. 71:356-365(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "SVOP, an evolutionarily conserved synaptic vesicle protein, suggests novel transport functions of synaptic vesicles."
    Janz R., Hofmann K., Suedhof T.C.
    J. Neurosci. 18:9269-9281(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.
  5. "SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family."
    Janz R., Suedhof T.C.
    Neuroscience 94:1279-1290(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Phosphorylation of synaptic vesicle protein 2 modulates binding to synaptotagmin."
    Pyle R.A., Schivell A.E., Hidaka H., Bajjalieh S.M.
    J. Biol. Chem. 275:17195-17200(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY CK1.
  7. "Brain synaptic junctional proteins at the acrosome of rat testicular germ cells."
    Redecker P., Kreutz M.R., Bockmann J., Gundelfinger E.D., Boeckers T.M.
    J. Histochem. Cytochem. 51:809-819(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "SV2A and SV2C are not vesicular Ca2+ transporters but control glucose-evoked granule recruitment."
    Iezzi M., Theander S., Janz R., Loze C., Wollheim C.B.
    J. Cell Sci. 118:5647-5660(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. Cited for: INTERACTION WITH SYT1.
  10. "SV2 is the protein receptor for botulinum neurotoxin A."
    Dong M., Yeh F., Tepp W.H., Dean C., Johnson E.A., Janz R., Chapman E.R.
    Science 312:592-596(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A BOTA RECEPTOR.

Entry informationi

Entry nameiSV2B_RAT
AccessioniPrimary (citable) accession number: Q63564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3