Q63556 (SPA3M_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine protease inhibitor A3M
Short name=Serpin A3M

Alternative name(s):
Serine protease inhibitor 2.4
Short name=SPI-2.4

Gene names

Name:

Serpina3m

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	412 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Secreted By similarity.
Domain	The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable By similarity. Variability within the reactive center loop (RCL) sequences of Serpina3 paralogs may determine target protease specificity.
Miscellaneous	The single human alpha1-antichymotrypsin gene (SERPINA3) is represented by a cluster of 6 individual rat paralogs.
Sequence similarities	Belongs to the serpin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of proteolysis Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	extracellular region Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 20	›20	Potential
	Chain	21 – 412	392	Serine protease inhibitor A3M		PRO_0000032420
Regions
	Region	360 – 386	27	RCL
Sites
	Site	374 – 375	2	Reactive bond By similarity
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	177	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q63556 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CC6F4DBA7F118CF1	FASTA	412	46,095
	10 20 30 40 50 60 FLLMAGICPA VLGFPDGTLG NDTLLHKDQD KGTQLDSLTL ESINTDFAFS LYKMLALKNP 70 80 90 100 110 120 DKNVVFSPLS ISAALAIVSL GAKGNTLEEI LEVLRFNLTE SYETDIHQGF GHLLQRLSQP 130 140 150 160 170 180 GDQVKIITGN ALFIDKNLQV LAEFQEKTRA LYQVEAFTAS FQQPRVTEKL INDSVRNQTQ 190 200 210 220 230 240 GKIQELVSGL KERTSMVLVN ILLFRGKWKV PFDPDYTFES EFYVDEKRSV KVSMMKIEEL 250 260 270 280 290 300 TTPYFRDEEL SCSVLELKYT GNSSALFILP DKGRMQQVEA SLQPETLKKW KDSLRPRKID 310 320 330 340 350 360 ELYLPRLSIS TDYSLEEVLP ELGIRDVFSQ QADLSRITGA KDLSVSQVVH KVVLDVNETG 370 380 390 400 410 TEAAAATGAN LVPRSGRPPM IVWFNRPFLI AVSHTHGQHI LFMAKVINPV GA

References

[1]	Inglis J.D. Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Expression patterns of murine antichymotrypsin-like genes reflect evolutionary divergence at the Serpina3 locus." Horvath A.J., Forsyth S.L., Coughlin P.B. J. Mol. Evol. 59:488-497(2004) [PubMed] [Europe PMC] [Abstract] Cited for: REGION RCL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69834 mRNA. Translation: CAA49488.1.
IPI	IPI01016420.
PIR	S31505.
UniGene	Rn.107050.
3D structure databases
ProteinModelPortal	Q63556.
SMR	Q63556. Positions 40-409.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000013175.
Protein family/group databases
MEROPS	I04.058.
Proteomic databases
PaxDb	Q63556.
PRIDE	Q63556.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:735068. rat.
Organism-specific databases
RGD	735068. Serpina3m.
Phylogenomic databases
eggNOG	COG4826.
HOGENOM	HOG000238521.
HOVERGEN	HBG005957.
InParanoid	Q63556.
OrthoDB	EOG4XH00Q.
Gene expression databases
ArrayExpress	Q63556.
Genevestigator	Q63556.
GermOnline	ENSRNOG00000009921. Rattus norvegicus.
Family and domain databases
InterPro	IPR023795. Protease_inhib_I4_serpin_CS. IPR023796. Serpin_dom. IPR000215. Serpin_fam. [Graphical view]
PANTHER	PTHR11461. PTHR11461. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPA3M_RAT

Accession

Primary (citable) accession number: Q63556

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program