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Protein

Synapsin-2

Gene

Syn2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: InterPro
  • calcium-dependent protein binding Source: RGD
  • catalytic activity Source: InterPro
  • cytoskeletal protein binding Source: RGD

GO - Biological processi

  • neurotransmitter secretion Source: ParkinsonsUK-UCL
  • regulation of neurotransmitter secretion Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Synapsin-2
Alternative name(s):
Synapsin II
Gene namesi
Name:Syn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3798. Syn2.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • synapse Source: ParkinsonsUK-UCL
  • synaptic vesicle Source: UniProtKB
  • synaptic vesicle membrane Source: ParkinsonsUK-UCL
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001830231 – 586Synapsin-2Add BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Phosphoserine; by PKA and CaMK11 Publication1
Modified residuei422PhosphothreonineCombined sources1
Modified residuei426PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-426 by MAPK1/ERK2 and/or MAPK3/ERK1 may play a role in noradrenaline secretion by sympathetic neurons (By similarity). Phosphorylation at Ser-10 dissociates synapsins from synaptic vesicles.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63537.
PRIDEiQ63537.

2D gel databases

World-2DPAGE0004:Q63537.

PTM databases

iPTMnetiQ63537.
PhosphoSitePlusiQ63537.
SwissPalmiQ63537.

Miscellaneous databases

PMAP-CutDBQ63537.

Interactioni

Subunit structurei

Interacts with CAPON.1 Publication

GO - Molecular functioni

  • calcium-dependent protein binding Source: RGD
  • cytoskeletal protein binding Source: RGD

Protein-protein interaction databases

BioGridi247859. 1 interactor.
IntActiQ63537. 1 interactor.
MINTiMINT-233013.
STRINGi10116.ENSRNOP00000011292.

Structurei

Secondary structure

1586
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi114 – 120Combined sources7
Helixi127 – 131Combined sources5
Turni137 – 139Combined sources3
Beta strandi140 – 147Combined sources8
Helixi149 – 151Combined sources3
Beta strandi152 – 157Combined sources6
Beta strandi162 – 168Combined sources7
Beta strandi170 – 179Combined sources10
Beta strandi182 – 186Combined sources5
Helixi200 – 208Combined sources9
Beta strandi213 – 215Combined sources3
Helixi217 – 222Combined sources6
Helixi226 – 240Combined sources15
Turni242 – 244Combined sources3
Beta strandi251 – 255Combined sources5
Helixi256 – 259Combined sources4
Beta strandi265 – 273Combined sources9
Turni276 – 279Combined sources4
Beta strandi280 – 283Combined sources4
Helixi286 – 299Combined sources14
Beta strandi303 – 307Combined sources5
Beta strandi311 – 320Combined sources10
Beta strandi323 – 332Combined sources10
Turni334 – 336Combined sources3
Beta strandi343 – 348Combined sources6
Helixi352 – 361Combined sources10
Helixi362 – 366Combined sources5
Beta strandi369 – 378Combined sources10
Beta strandi383 – 389Combined sources7
Helixi400 – 418Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I7LX-ray2.35A/B113-421[»]
1I7NX-ray1.90A/B113-421[»]
ProteinModelPortaliQ63537.
SMRiQ63537.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63537.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 29AAdd BLAST29
Regioni33 – 113B; linkerAdd BLAST81
Regioni114 – 421C; actin-binding and synaptic-vesicle bindingAdd BLAST308
Regioni422 – 458G; Pro-rich linkerAdd BLAST37
Regioni459 – 537H; Pro/Ser-rich linkerAdd BLAST79
Regioni538 – 586EAdd BLAST49

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi487 – 499Poly-SerAdd BLAST13
Compositional biasi515 – 520Poly-Ser6

Domaini

The A region binds phospholipids with a preference for negatively charged species.

Sequence similaritiesi

Belongs to the synapsin family.Curated

Phylogenomic databases

eggNOGiKOG3895. Eukaryota.
ENOG410XQH5. LUCA.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiQ63537.
KOiK19941.
PhylomeDBiQ63537.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 1 hit.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform IIa (identifier: Q63537-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMNFLRRRLS DSSFIANLPN GYMTDLQRPE PQQPPPAPGP GTATASAATS
60 70 80 90 100
AASPGPERRP PPAQAPAPQP APQPAPTPSV GSSFFSSLSQ AVKQTAASAG
110 120 130 140 150
LVDAPAPSAA SRKAKVLLVV DEPHTDWAKC FRGKKILGDY DIKVEQAEFS
160 170 180 190 200
ELNLVAHADG TYAVDMQVLR NGTKVVRSFR PDFVLIRQHA FGMAENEDFR
210 220 230 240 250
HLVIGMQYAG LPSINSLESI YNFCDKPWVF AQMVAIFKTL GGEKFPLIEQ
260 270 280 290 300
TYYPNHREML TLPTFPVVVK IGHAHSGMGK VKVENHYDFQ DIASVVALTQ
310 320 330 340 350
TYATAEPFID AKYDIRVQKI GNNYKAYMRT SISGNWKTNT GSAMLEQIAM
360 370 380 390 400
SDRYKLWVDA CSEMFGGLDI CAVKAVHGKD GKDYIFEVMD CSMPLIGEHQ
410 420 430 440 450
VEDRQLITDL VISKMNQLLS RTPALSPQRP LTTQQPQSGT LKEPDSSKTP
460 470 480 490 500
PQRPAPQGGP GQPQGMQPPG KVLPPRRLPS GPSLPPSSSS SSSSSSSSSA
510 520 530 540 550
PQRPGGPTST QVNASSSSNS LAEPQAPQAA PPQKPQPHPQ LNKSQSLTNA
560 570 580
FSFSESSFFR SSANEDEAKA ETIRSLRKSF ASLFSD
Length:586
Mass (Da):63,457
Last modified:November 1, 1996 - v1
Checksum:i630B7071B2F38857
GO
Isoform IIb (identifier: Q63537-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     459-479: GPGQPQGMQPPGKVLPPRRLP → CLQYILNCNGIAVGPKQVQAS
     480-586: Missing.

Show »
Length:479
Mass (Da):52,455
Checksum:i9A495D706EA65614
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006322459 – 479GPGQP…PRRLP → CLQYILNCNGIAVGPKQVQA S in isoform IIb. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_006323480 – 586Missing in isoform IIb. 1 PublicationAdd BLAST107

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27925 mRNA. Translation: AAA42100.1.
M27926 mRNA. Translation: AAA42101.1.
PIRiC30411.
RefSeqiNP_001029192.1. NM_001034020.1. [Q63537-1]
NP_062032.1. NM_019159.1. [Q63537-2]
UniGeneiRn.506.

Genome annotation databases

GeneIDi29179.
KEGGirno:29179.
UCSCiRGD:3798. rat. [Q63537-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27925 mRNA. Translation: AAA42100.1.
M27926 mRNA. Translation: AAA42101.1.
PIRiC30411.
RefSeqiNP_001029192.1. NM_001034020.1. [Q63537-1]
NP_062032.1. NM_019159.1. [Q63537-2]
UniGeneiRn.506.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I7LX-ray2.35A/B113-421[»]
1I7NX-ray1.90A/B113-421[»]
ProteinModelPortaliQ63537.
SMRiQ63537.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247859. 1 interactor.
IntActiQ63537. 1 interactor.
MINTiMINT-233013.
STRINGi10116.ENSRNOP00000011292.

PTM databases

iPTMnetiQ63537.
PhosphoSitePlusiQ63537.
SwissPalmiQ63537.

2D gel databases

World-2DPAGE0004:Q63537.

Proteomic databases

PaxDbiQ63537.
PRIDEiQ63537.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29179.
KEGGirno:29179.
UCSCiRGD:3798. rat. [Q63537-1]

Organism-specific databases

CTDi6854.
RGDi3798. Syn2.

Phylogenomic databases

eggNOGiKOG3895. Eukaryota.
ENOG410XQH5. LUCA.
HOGENOMiHOG000231323.
HOVERGENiHBG016354.
InParanoidiQ63537.
KOiK19941.
PhylomeDBiQ63537.

Miscellaneous databases

EvolutionaryTraceiQ63537.
PMAP-CutDBQ63537.
PROiQ63537.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR001359. Synapsin.
IPR020898. Synapsin_ATP-bd_dom.
IPR019735. Synapsin_CS.
IPR019736. Synapsin_P_site.
IPR020897. Synapsin_pre-ATP-grasp_dom.
[Graphical view]
PANTHERiPTHR10841. PTHR10841. 1 hit.
PfamiPF02078. Synapsin. 1 hit.
PF02750. Synapsin_C. 1 hit.
PF10581. Synapsin_N. 1 hit.
[Graphical view]
PRINTSiPR01368. SYNAPSIN.
SUPFAMiSSF52440. SSF52440. 1 hit.
PROSITEiPS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYN2_RAT
AccessioniPrimary (citable) accession number: Q63537
Secondary accession number(s): Q9Z1H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.