Reviewed, UniProtKB/Swiss-Prot Q634K8 (AROE_BACCZ)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BCE33L4080

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000021261
Regions
	Nucleotide binding	127 – 131	5	NADP By similarity
Sites
	Active site	66	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q634K8-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: AA14CF000F58EDA9	FASTA	277	30,205
	10 20 30 40 50 60 MKQLYGVIGN PIGHSLSPVM HNDAFEHLNM DAHYHAFLVK EEVLGEAVRG LKALGISGFN 70 80 90 100 110 120 VTTPHKVAIM DYLDEIDPLA KQIGAVNTVV HKDGKLIGYN TDGIGFVRAL QSISNEPLQE 130 140 150 160 170 180 KRILLLGAGG ASRAIYFSLA DVGVKEIDVA NRTVDKAKEL IAACTATVHS VALSLEKATK 190 200 210 220 230 240 EQGNYDIIIQ TTTIGMHPRV EHTPLQISSL KKGTIVSDII YNPFETKILC EAKEQGAIIQ 250 260 270 NGIDMFVYQG ALAFEMWTGC VPNIERMKQL VIRKLGG

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000001 Genomic DNA. Translation: AAU16190.1.
RefSeq	YP_085659.1.
3D structure databases
HSSP	HSSP built from PDB template 1NVT based on UniProtKB Q58484.
SMR	Q634K8. Positions 1-275.
ModBase	Search...
Protein-protein interaction databases
STRING	Q634K8.
Genome annotation databases
GeneID	3026764.
GenomeReviews	Gene locus BCE33L4080 in contig CP000001_GR.
KEGG	bcz:BCZK4080.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	IVSDIIY.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L4080-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BACCZ

Accession

Primary (citable) accession number: Q634K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)