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Q63472 (KCNH1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily H member 1
Alternative name(s):
Ether-a-go-go potassium channel 1
Short name=EAG channel 1
Short name=EAG1
Short name=r-eag
Voltage-gated potassium channel subunit Kv10.1
Gene names
Name:Kcnh1
Synonyms:Eag
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length962 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts By similarity.

Subunit structure

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH5/EAG2 By similarity. Interacts with ALG10B. Ref.3

Subcellular location

Cell membrane By similarity; Multi-pass membrane protein. Nucleus inner membrane By similarity; Multi-pass membrane protein. Note: Perinuclear KCNH1 is located to NPC-free islands By similarity.

Tissue specificity

Detected in brain, but not in the other tissues tested. Expression is highest in granular cells of the dentate gyrus, in hippocampus CA3 pyramidal cells, and in cerebellar granule cells. Found in pituitary. Ref.4

Domain

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Ref.2

Sequence similarities

Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.1/KCNH1 sub-subfamily. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Rabep1O355502EBI-7991592,EBI-7991542

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 962962Potassium voltage-gated channel subfamily H member 1
PRO_0000053996

Regions

Topological domain1 – 220220Cytoplasmic Potential
Transmembrane221 – 24121Helical; Name=Segment S1; Potential
Topological domain242 – 2487Extracellular Potential
Transmembrane249 – 26921Helical; Name=Segment S2; Potential
Topological domain270 – 29425Cytoplasmic Potential
Transmembrane295 – 31521Helical; Name=Segment S3; Potential
Topological domain316 – 3227Extracellular Potential
Transmembrane323 – 34321Helical; Voltage-sensor; Name=Segment S4; Potential
Topological domain344 – 3496Cytoplasmic Potential
Transmembrane350 – 37021Helical; Name=Segment S5; Potential
Topological domain371 – 42151Extracellular Potential
Intramembrane422 – 44221Pore-forming; Name=Segment H5; Potential
Topological domain443 – 4508Extracellular Potential
Transmembrane451 – 47121Helical; Name=Segment S6; Potential
Topological domain472 – 962491Cytoplasmic Potential
Domain14 – 9481PAS
Domain93 – 14553PAC
Nucleotide binding554 – 671118cNMP
Region646 – 74398Calmodulin-binding By similarity
Region897 – 93741CAD (involved in subunit assembly)
Motif436 – 4416Selectivity filter By similarity

Amino acid modifications

Glycosylation3881N-linked (GlcNAc...) Potential
Glycosylation4061N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q63472 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 69DF4CE2DC435608

FASTA962108,291
        10         20         30         40         50         60 
MTMAGGRRGL VAPQNTFLEN IVRRSNDTNF VLGNAQIVDW PIVYSNDGFC KLSGYHRAEV 

        70         80         90        100        110        120 
MQKSSACSFM YGELTDKDTV EKVRQTFENY EMNSFEILMY KKNRTPVWFF VKIAPIRNEQ 

       130        140        150        160        170        180 
DKVVLFLCTF SDITAFKQPI EDDSCKGWGK FARLTRALTS SRGVLQQLAP SVQKGENVHK 

       190        200        210        220        230        240 
HSRLAEVLQL GSDILPQYKQ EAPKTPPHII LHYCVFKTTW DWIILILTFY TAILVPYNVS 

       250        260        270        280        290        300 
FKTRQNNVAW LVVDSIVDVI FLVDIVLNFH TTFVGPAGEV ISDPKLIRMN YLKTWFVIDL 

       310        320        330        340        350        360 
LSCLPYDVIN AFENVDEGIS SLFSSLKVVR LLRLGRVARK LDHYIEYGAA VLVLLVCVFG 

       370        380        390        400        410        420 
LAAHWMACIW YSIGDYEIFD EDTKTIRNNS WLYQLALDIG TPYQFNGSGS GKWEGGPSKN 

       430        440        450        460        470        480 
SVYISSLYFT MTSLTSVGFG NIAPSTDIEK IFAVAIMMIG SLLYATIFGN VTTIFQQMYA 

       490        500        510        520        530        540 
NTNRYHEMLN SVRDFLKLYQ VPKGLSERVM DYIVSTWSMS RGIDTEKVLQ ICPKDMRADI 

       550        560        570        580        590        600 
CVHLNRKVFK EHPAFRLASD GCLRALAMEF QTVHCAPGDL IYHAGESVDS LCFVVSGSLE 

       610        620        630        640        650        660 
VIQDDEVVAI LGKGDVFGDV FWKEATLAQS CANVRALTYC DLHVIKRDAL QKVLEFYTAF 

       670        680        690        700        710        720 
SHSFSRNLIL TYNLRKRIVF RKISDVKREE EERMKRKNEA PLILPPDHPV RRLFQRFRQQ 

       730        740        750        760        770        780 
KEARLAAERG GRDLDDLDVE KGNALTDHTS ANHSLVKASV VTVRESPATP VSFQAASTST 

       790        800        810        820        830        840 
VSDHAKLHAP GSECLGPKAG GGDPAKRKGW ARFKDACGKG EDWNKVSKAE SMETLPERTK 

       850        860        870        880        890        900 
ASGEATLKKT DSCDSGITKS DLRLDNVGEA RSPQDRSPIL AEVKHSFYPI PEQTLQATVL 

       910        920        930        940        950        960 
EVKHELKEDI KALNAKMTSI EKQLSEILRI LMSRGSSQSP QDTCEVSRPQ SPESDRDIFG 


AS 

« Hide

References

[1]"Functional expression of a rat homologue of the voltage gated ether a go-go potassium channel reveals differences in selectivity and activation kinetics between the Drosophila channel and its mammalian counterpart."
Ludwig J., Terlau H., Wunder F., Brueggemann A., Pardo L.A., Marquardt A., Stuehmer W., Pongs O.
EMBO J. 13:4451-4458(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Cerebellum.
[2]"Carboxy-terminal domain mediates assembly of the voltage-gated rat ether-a-go-go potassium channel."
Ludwig J., Owen D., Pongs O.
EMBO J. 16:6337-6345(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN CAD.
[3]"KCR1, a membrane protein that facilitates functional expression of non-inactivating K+ currents associates with rat EAG voltage-dependent K+ channels."
Hoshi N., Takahashi H., Shahidullah M., Yokoyama S., Higashida H.
J. Biol. Chem. 273:23080-23085(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ALG10B.
Strain: Wistar.
Tissue: Cerebellum.
[4]"Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary."
Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.
J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z34264 mRNA. Translation: CAA84018.1.
PIRI53197.
RefSeqNP_113930.1. NM_031742.1.
XP_006250553.1. XM_006250491.1.
UniGeneRn.11071.

3D structure databases

ProteinModelPortalQ63472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ63472. 2 interactions.
MINTMINT-8386100.
STRING10116.ENSRNOP00000005209.

Chemistry

GuidetoPHARMACOLOGY570.

Protein family/group databases

TCDB1.A.1.20.4. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteQ63472.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000005209; ENSRNOP00000005209; ENSRNOG00000003841.
GeneID65198.
KEGGrno:65198.

Organism-specific databases

CTD3756.
RGD68398. Kcnh1.

Phylogenomic databases

eggNOGCOG2202.
GeneTreeENSGT00680000099663.
HOVERGENHBG101348.
KOK04904.
OMAHAKLHAP.
OrthoDBEOG7QG43V.
PhylomeDBQ63472.

Gene expression databases

GenevestigatorQ63472.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003949. K_chnl_volt-dep_EAG.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSPR01463. EAGCHANLFMLY.
PR01464. EAGCHANNEL.
SMARTSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio614144.
PROQ63472.

Entry information

Entry nameKCNH1_RAT
AccessionPrimary (citable) accession number: Q63472
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families