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Q63472

- KCNH1_RAT

UniProt

Q63472 - KCNH1_RAT

Protein

Potassium voltage-gated channel subfamily H member 1

Gene

Kcnh1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi554 – 671118cNMPAdd
    BLAST

    GO - Molecular functioni

    1. phosphorelay sensor kinase activity Source: InterPro
    2. protein binding Source: IntAct
    3. voltage-gated potassium channel activity Source: RefGenome

    GO - Biological processi

    1. potassium ion transmembrane transport Source: RefGenome
    2. regulation of membrane potential Source: RefGenome

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Calmodulin-binding, Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199159. Voltage gated Potassium channels.

    Protein family/group databases

    TCDBi1.A.1.20.4. the voltage-gated ion channel (vic) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily H member 1
    Alternative name(s):
    Ether-a-go-go potassium channel 1
    Short name:
    EAG channel 1
    Short name:
    EAG1
    Short name:
    r-eag
    Voltage-gated potassium channel subunit Kv10.1
    Gene namesi
    Name:Kcnh1
    Synonyms:Eag
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi68398. Kcnh1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein. Nucleus inner membrane By similarity; Multi-pass membrane protein
    Note: Perinuclear KCNH1 is located to NPC-free islands.By similarity

    GO - Cellular componenti

    1. integral component of plasma membrane Source: RefGenome
    2. nuclear inner membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 962962Potassium voltage-gated channel subfamily H member 1PRO_0000053996Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    PTM databases

    PhosphoSiteiQ63472.

    Expressioni

    Tissue specificityi

    Detected in brain, but not in the other tissues tested. Expression is highest in granular cells of the dentate gyrus, in hippocampus CA3 pyramidal cells, and in cerebellar granule cells. Found in pituitary.1 Publication

    Gene expression databases

    GenevestigatoriQ63472.

    Interactioni

    Subunit structurei

    The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH5/EAG2 By similarity. Interacts with ALG10B.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Rabep1O355502EBI-7991592,EBI-7991542

    Protein-protein interaction databases

    IntActiQ63472. 2 interactions.
    MINTiMINT-8386100.
    STRINGi10116.ENSRNOP00000005209.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63472.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 220220CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini242 – 2487ExtracellularSequence Analysis
    Topological domaini270 – 29425CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini316 – 3227ExtracellularSequence Analysis
    Topological domaini344 – 3496CytoplasmicSequence Analysis
    Topological domaini371 – 42151ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini443 – 4508ExtracellularSequence Analysis
    Topological domaini472 – 962491CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei422 – 44221Pore-forming; Name=Segment H5Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei221 – 24121Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei249 – 26921Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei295 – 31521Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei323 – 34321Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei350 – 37021Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei451 – 47121Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 9481PASPROSITE-ProRule annotationAdd
    BLAST
    Domaini93 – 14553PACPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni646 – 74398Calmodulin-bindingBy similarityAdd
    BLAST
    Regioni897 – 93741CAD (involved in subunit assembly)Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi436 – 4416Selectivity filterBy similarity

    Domaini

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.1 Publication

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
    Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2202.
    GeneTreeiENSGT00680000099663.
    HOVERGENiHBG101348.
    KOiK04904.
    OMAiHAKLHAP.
    OrthoDBiEOG7QG43V.
    PhylomeDBiQ63472.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR003949. K_chnl_volt-dep_EAG.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF13426. PAS_9. 1 hit.
    [Graphical view]
    PRINTSiPR01463. EAGCHANLFMLY.
    PR01464. EAGCHANNEL.
    SMARTiSM00100. cNMP. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    SSF55785. SSF55785. 1 hit.
    TIGRFAMsiTIGR00229. sensory_box. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    PS50113. PAC. 1 hit.
    PS50112. PAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q63472-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTMAGGRRGL VAPQNTFLEN IVRRSNDTNF VLGNAQIVDW PIVYSNDGFC    50
    KLSGYHRAEV MQKSSACSFM YGELTDKDTV EKVRQTFENY EMNSFEILMY 100
    KKNRTPVWFF VKIAPIRNEQ DKVVLFLCTF SDITAFKQPI EDDSCKGWGK 150
    FARLTRALTS SRGVLQQLAP SVQKGENVHK HSRLAEVLQL GSDILPQYKQ 200
    EAPKTPPHII LHYCVFKTTW DWIILILTFY TAILVPYNVS FKTRQNNVAW 250
    LVVDSIVDVI FLVDIVLNFH TTFVGPAGEV ISDPKLIRMN YLKTWFVIDL 300
    LSCLPYDVIN AFENVDEGIS SLFSSLKVVR LLRLGRVARK LDHYIEYGAA 350
    VLVLLVCVFG LAAHWMACIW YSIGDYEIFD EDTKTIRNNS WLYQLALDIG 400
    TPYQFNGSGS GKWEGGPSKN SVYISSLYFT MTSLTSVGFG NIAPSTDIEK 450
    IFAVAIMMIG SLLYATIFGN VTTIFQQMYA NTNRYHEMLN SVRDFLKLYQ 500
    VPKGLSERVM DYIVSTWSMS RGIDTEKVLQ ICPKDMRADI CVHLNRKVFK 550
    EHPAFRLASD GCLRALAMEF QTVHCAPGDL IYHAGESVDS LCFVVSGSLE 600
    VIQDDEVVAI LGKGDVFGDV FWKEATLAQS CANVRALTYC DLHVIKRDAL 650
    QKVLEFYTAF SHSFSRNLIL TYNLRKRIVF RKISDVKREE EERMKRKNEA 700
    PLILPPDHPV RRLFQRFRQQ KEARLAAERG GRDLDDLDVE KGNALTDHTS 750
    ANHSLVKASV VTVRESPATP VSFQAASTST VSDHAKLHAP GSECLGPKAG 800
    GGDPAKRKGW ARFKDACGKG EDWNKVSKAE SMETLPERTK ASGEATLKKT 850
    DSCDSGITKS DLRLDNVGEA RSPQDRSPIL AEVKHSFYPI PEQTLQATVL 900
    EVKHELKEDI KALNAKMTSI EKQLSEILRI LMSRGSSQSP QDTCEVSRPQ 950
    SPESDRDIFG AS 962
    Length:962
    Mass (Da):108,291
    Last modified:November 1, 1996 - v1
    Checksum:i69DF4CE2DC435608
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34264 mRNA. Translation: CAA84018.1.
    PIRiI53197.
    RefSeqiNP_113930.1. NM_031742.1.
    XP_006250553.1. XM_006250491.1.
    UniGeneiRn.11071.

    Genome annotation databases

    EnsembliENSRNOT00000005209; ENSRNOP00000005209; ENSRNOG00000003841.
    GeneIDi65198.
    KEGGirno:65198.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34264 mRNA. Translation: CAA84018.1 .
    PIRi I53197.
    RefSeqi NP_113930.1. NM_031742.1.
    XP_006250553.1. XM_006250491.1.
    UniGenei Rn.11071.

    3D structure databases

    ProteinModelPortali Q63472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q63472. 2 interactions.
    MINTi MINT-8386100.
    STRINGi 10116.ENSRNOP00000005209.

    Chemistry

    GuidetoPHARMACOLOGYi 570.

    Protein family/group databases

    TCDBi 1.A.1.20.4. the voltage-gated ion channel (vic) superfamily.

    PTM databases

    PhosphoSitei Q63472.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000005209 ; ENSRNOP00000005209 ; ENSRNOG00000003841 .
    GeneIDi 65198.
    KEGGi rno:65198.

    Organism-specific databases

    CTDi 3756.
    RGDi 68398. Kcnh1.

    Phylogenomic databases

    eggNOGi COG2202.
    GeneTreei ENSGT00680000099663.
    HOVERGENi HBG101348.
    KOi K04904.
    OMAi HAKLHAP.
    OrthoDBi EOG7QG43V.
    PhylomeDBi Q63472.

    Enzyme and pathway databases

    Reactomei REACT_199159. Voltage gated Potassium channels.

    Miscellaneous databases

    NextBioi 614144.
    PROi Q63472.

    Gene expression databases

    Genevestigatori Q63472.

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR003949. K_chnl_volt-dep_EAG.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF13426. PAS_9. 1 hit.
    [Graphical view ]
    PRINTSi PR01463. EAGCHANLFMLY.
    PR01464. EAGCHANNEL.
    SMARTi SM00100. cNMP. 1 hit.
    SM00086. PAC. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    SSF55785. SSF55785. 1 hit.
    TIGRFAMsi TIGR00229. sensory_box. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    PS50113. PAC. 1 hit.
    PS50112. PAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional expression of a rat homologue of the voltage gated ether a go-go potassium channel reveals differences in selectivity and activation kinetics between the Drosophila channel and its mammalian counterpart."
      Ludwig J., Terlau H., Wunder F., Brueggemann A., Pardo L.A., Marquardt A., Stuehmer W., Pongs O.
      EMBO J. 13:4451-4458(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Cerebellum.
    2. "Carboxy-terminal domain mediates assembly of the voltage-gated rat ether-a-go-go potassium channel."
      Ludwig J., Owen D., Pongs O.
      EMBO J. 16:6337-6345(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN CAD.
    3. "KCR1, a membrane protein that facilitates functional expression of non-inactivating K+ currents associates with rat EAG voltage-dependent K+ channels."
      Hoshi N., Takahashi H., Shahidullah M., Yokoyama S., Higashida H.
      J. Biol. Chem. 273:23080-23085(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ALG10B.
      Strain: Wistar.
      Tissue: Cerebellum.
    4. "Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary."
      Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.
      J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiKCNH1_RAT
    AccessioniPrimary (citable) accession number: Q63472
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3