Reviewed,
UniProtKB/Swiss-Prot Q63470 (DYR1A_RAT)
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November 3, 2009.
Version 87.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Dual specificity tyrosine-phosphorylation-regulated kinase 1A EC=2.7.12.2 Alternative name(s): Protein kinase minibrain homolog Short name=MNBH RP86 Dual specificity YAK1-related kinase | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 763 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates. Ref.1 Ref.3 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Enzyme regulation | Inhibited by RANBP9 By similarity. |
| Subunit structure | Interacts with RANBP9. Interacts with RAD54L2/ARIP4 By similarity. Interacts with WDR68 By similarity. |
| Subcellular location | |
| Tissue specificity | |
| Post-translational modification | Autophosphorylated on tyrosine residues. Ref.1 |
| Sequence similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: Q63470-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: Q63470-2) The sequence of this isoform differs from the canonical sequence as follows: 70-78: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 763 | 763 | Dual specificity tyrosine-phosphorylation-regulated kinase 1A | PRO_0000085933 | |||||
Regions | |||||||||
| Domain | 159 – 479 | 321 | Protein kinase | ||||||
| Nucleotide binding | 165 – 173 | 9 | ATP By similarity | ||||||
| Motif | 117 – 134 | 18 | Bipartite nuclear localization signal Potential | ||||||
| Compositional bias | 509 – 515 | 7 | Poly-Ser | ||||||
| Compositional bias | 599 – 602 | 4 | Poly-His | ||||||
| Compositional bias | 607 – 619 | 13 | Poly-His | ||||||
| Compositional bias | 656 – 672 | 17 | Ser/Thr-rich | ||||||
| Compositional bias | 664 – 671 | 8 | Poly-Ser | ||||||
Sites | |||||||||
| Active site | 287 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 188 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 145 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 219 | 1 | Phosphotyrosine; by autocatalysis Ref.1 | ||||||
| Modified residue | 319 | 1 | Phosphotyrosine; by autocatalysis Ref.1 | ||||||
| Modified residue | 321 | 1 | Phosphotyrosine; by autocatalysis Ref.1 | ||||||
| Modified residue | 748 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 758 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 70 – 78 | 9 | Missing in isoform Short. | VSP_004924 | |||||
Experimental info | |||||||||
| Mutagenesis | 188 | 1 | K → R: Reduced kinase activity but retains some kinase activity towards histones. Ref.1 | ||||||
| Mutagenesis | 219 | 1 | Y → F: Reduced autophosphorylation on tyrosine, but retains some kinase activity towards histones. Ref.1 | ||||||
| Mutagenesis | 319 | 1 | Y → F: Suppressed autophosphorylation on tyrosine and reduced tyrosine, threonine and serine kinase activity towards histones; when associated with F-321. Ref.1 | ||||||
| Mutagenesis | 321 | 1 | Y → F: Suppressed autophosphorylation on tyrosine and reduced tyrosine, threonine and serine kinase activity towards histones; when associated with F-319. Ref.1 | ||||||
Sequences
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References
| [1] | "Dyrk, a dual specificity protein kinase with unique structural features whose activity is dependent on tyrosine residues between subdomains VII and VIII." Kentrup H., Becker W., Heukelbach J., Wilmes A., Schuermann A., Huppertz C., Kainulainen H., Joost H.-G. J. Biol. Chem. 271:3488-3495(1996) [PubMed: 8631952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), FUNCTION, TISSUE SPECIFICITY, AUTOPHOSPHORYLATION AT TYR-219; TYR-319 AND TYR-321, MUTAGENESIS OF LYS-188; TYR-219; TYR-319 AND TYR-321. Strain: Sprague-Dawley. Tissue: Brain. |
| [2] | Kentrup H. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. |
| [3] | "Sequence characteristics, subcellular localization, and substrate specificity of DYRK-related kinases, a novel family of dual specificity protein kinases." Becker W., Weber Y., Wetzel K., Eirmbter K., Tejedor F.J., Joost H.-G. J. Biol. Chem. 273:25893-25902(1998) [PubMed: 9748265] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X79769 mRNA. Translation: CAA56164.1. | |
| IPI | IPI00209116. IPI00231347. |
| RefSeq | NP_036923.1. |
| UniGene | Rn.9354 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1I09 based on UniProtKB P49841. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q63470. |
PTM databases | |
| PhosphoSite | Q63470. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000050342; ENSRNOP00000042446; ENSRNOG00000001662; Rattus norvegicus. [Genome view] |
| GeneID | 25255. |
| KEGG | rno:25255. |
| UCSC | NM_012791. rat. |
Organism-specific databases | |
| CTD | 25255. |
| RGD | 2528. Dyrk1a. |
Phylogenomic databases | |
| HOVERGEN | Q63470. |
Enzyme and pathway databases | |
| BRENDA | 2.7.12.1. 248. 2.7.12.2. 248. |
Gene expression databases | |
| ArrayExpress | Q63470. |
| Genevestigator | Q63470. |
| GermOnline | ENSRNOG00000001662. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 605887. |
Entry information
| Entry name | DYR1A_RAT | ||||||||
| Accession | Primary (citable) accession number: Q63470 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
