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Protein

Mitogen-activated protein kinase 4

Gene

Mapk4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by phosphorylation in the activation loop.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23ATPPROSITE-ProRule annotation1
Active sitei123Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi‹1 – 8ATPPROSITE-ProRule annotation›8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 4 (EC:2.7.11.24)
Short name:
MAP kinase 4
Short name:
MAPK 4
Alternative name(s):
Extracellular signal-regulated kinase 4
Short name:
ERK-4
MAP kinase isoform p63
Short name:
p63-MAPK
MNK2
Gene namesi
Name:Mapk4
Synonyms:Prkm4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3047. Mapk4.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Translocates to the cytoplasm following interaction with MAPKAPK5.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000186256‹1 – ›274Mitogen-activated protein kinase 4Add BLAST›274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei160PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PAK1, PAK2 and PAK3 in the activation loop resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63454.
PRIDEiQ63454.

PTM databases

iPTMnetiQ63454.

Expressioni

Tissue specificityi

Exclusively detected in the brain, where expression is restricted to the choroid plexus and hippocampus, and to a lesser extent in lung.

Interactioni

Subunit structurei

Homodimer. Heterodimer with ERK3/MAPK6. Interacts with MAPKAPK5 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ63454. 1 interactor.
MINTiMINT-7138709.
STRINGi10116.ENSRNOP00000050164.

Structurei

3D structure databases

ProteinModelPortaliQ63454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini‹1 – ›274Protein kinasePROSITE-ProRule annotationAdd BLAST›274

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi160 – 162SEG motif3

Domaini

In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233020.
HOVERGENiHBG014652.
InParanoidiQ63454.
PhylomeDBiQ63454.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR008350. MAPK_ERK3/4.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01771. ERK3ERK4MAPK.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q63454-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GCGGNGLVLS ATDSRACRKV AVKKIVLSDA RSMKHALREI KIIRRLDHDN
60 70 80 90 100
IVKVYEVLGP KGSDLQGELF KFSVAYIVQE YMETDLACLL EQGTLTEEHA
110 120 130 140 150
KLFMYQLLRG LKYIHSANVL HRDLKPANIF ISTEDLVLKI GDFGLARIAD
160 170 180 190 200
QHYSHKGYLS EGLVTKWYRS PRLLLSPNNY TKAIDMWAAG CILAEMLTGK
210 220 230 240 250
MLFAGAHELE QMQLILDTIP VVREEDKEEL LRVMPSFVSS TWEVKRPLRK
260 270
LLPDVNREAI DFLEKILTFS PMDR
Length:274
Mass (Da):31,162
Last modified:November 1, 1996 - v1
Checksum:iD98E3079487AB51F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-terminal residuei2741

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21935 mRNA. Translation: CAA79929.1.
PIRiS33178.
UniGeneiRn.11731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21935 mRNA. Translation: CAA79929.1.
PIRiS33178.
UniGeneiRn.11731.

3D structure databases

ProteinModelPortaliQ63454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63454. 1 interactor.
MINTiMINT-7138709.
STRINGi10116.ENSRNOP00000050164.

PTM databases

iPTMnetiQ63454.

Proteomic databases

PaxDbiQ63454.
PRIDEiQ63454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi3047. Mapk4.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233020.
HOVERGENiHBG014652.
InParanoidiQ63454.
PhylomeDBiQ63454.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR008350. MAPK_ERK3/4.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01771. ERK3ERK4MAPK.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMK04_RAT
AccessioniPrimary (citable) accession number: Q63454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.