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Q63454 (MK04_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 4

Short name=MAP kinase 4
Short name=MAPK 4
EC=2.7.11.24
Alternative name(s):
Extracellular signal-regulated kinase 4
Short name=ERK-4
MAP kinase isoform p63
Short name=p63-MAPK
MNK2
Gene names
Name:Mapk4
Synonyms:Prkm4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length274 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by phosphorylation in the activation loop By similarity.

Subunit structure

Homodimer. Heterodimer with ERK3/MAPK6. Interacts with MAPKAPK5 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Translocates to the cytoplasm following interaction with MAPKAPK5 By similarity.

Tissue specificity

Exclusively detected in the brain, where expression is restricted to the choroid plexus and hippocampus, and to a lesser extent in lung.

Domain

In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases By similarity.

Post-translational modification

Phosphorylated by PAK1, PAK2 and PAK3 in the activation loop resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›274›274Mitogen-activated protein kinase 4
PRO_0000186256

Regions

Domain‹1 – ›274›274Protein kinase
Nucleotide binding‹1 – 8›8ATP By similarity
Motif160 – 1623SEG motif

Sites

Active site1231Proton acceptor By similarity
Binding site231ATP By similarity

Amino acid modifications

Modified residue1601Phosphoserine; by PAK1, PAK2 and PAK3 By similarity

Experimental info

Non-terminal residue11
Non-terminal residue2741

Sequences

Sequence LengthMass (Da)Tools
Q63454 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D98E3079487AB51F

FASTA27431,162
        10         20         30         40         50         60 
GCGGNGLVLS ATDSRACRKV AVKKIVLSDA RSMKHALREI KIIRRLDHDN IVKVYEVLGP 

        70         80         90        100        110        120 
KGSDLQGELF KFSVAYIVQE YMETDLACLL EQGTLTEEHA KLFMYQLLRG LKYIHSANVL 

       130        140        150        160        170        180 
HRDLKPANIF ISTEDLVLKI GDFGLARIAD QHYSHKGYLS EGLVTKWYRS PRLLLSPNNY 

       190        200        210        220        230        240 
TKAIDMWAAG CILAEMLTGK MLFAGAHELE QMQLILDTIP VVREEDKEEL LRVMPSFVSS 

       250        260        270 
TWEVKRPLRK LLPDVNREAI DFLEKILTFS PMDR 

« Hide

References

[1]"Isolation of a cDNA encoding the rat MAP-kinase homolog of human p63mapk."
Garcia J.I., Zalba G., Detera-Wadleigh S.D., de Miguel C.
Mamm. Genome 7:810-814(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Corpus striatum.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z21935 mRNA. Translation: CAA79929.1.
PIRS33178.
UniGeneRn.11731.

3D structure databases

ProteinModelPortalQ63454.
SMRQ63454. Positions 6-274.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ63454. 1 interaction.
MINTMINT-7138709.
STRING10116.ENSRNOP00000050164.

Proteomic databases

PaxDbQ63454.
PRIDEQ63454.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD3047. Mapk4.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233020.
HOVERGENHBG014652.
InParanoidQ63454.
PhylomeDBQ63454.

Gene expression databases

GenevestigatorQ63454.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR008350. MAPK_ERK3/4.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01771. ERK3ERK4MAPK.
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ63454.

Entry information

Entry nameMK04_RAT
AccessionPrimary (citable) accession number: Q63454
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families