Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Periaxin

Gene

Prx

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffolding protein that functions as part of a dystroglycan complex in Schwann cells, and as part of EZR and AHNAK-containing complexes in eye lens fiber cells. Required for the maintenance of the peripheral myelin sheath that is essential for normal transmission of nerve impulses and normal perception of sensory stimuli. Required for normal transport of MBP mRNA from the perinuclear to the paranodal regions. Required for normal remyelination after nerve injury. Required for normal elongation of Schwann cells and normal length of the internodes between the nodes of Ranvier. The demyelinated nodes of Ranvier permit saltatory transmission of nerve impulses; shorter internodes cause slower transmission of nerve impulses. Required for the formation of appositions between the abaxonal surface of the myelin sheath and the Schwann cell plasma membrane; the Schwann cell cytoplasm is restricted to regions between these appositions. Required for the formation of Cajal bands and of Schmidt-Lanterman incisures that correspond to short, cytoplasm-filled regions on myelinated nerves. Recruits DRP2 to the Schwann cell plasma membrane. Required for normal protein composition of the eye lens fiber cell plasma membrane and normal eye lens fiber cell morphology.By similarity

GO - Biological processi

  • axon ensheathment Source: RGD
  • nerve development Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Periaxin1 Publication
Gene namesi
Name:Prx
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619960. Prx.

Subcellular locationi

Isoform 1 :
Isoform 2 :
  • Cytoplasm By similarity
  • Cell membrane By similarity
  • Cell junction By similarity

  • Note: Colocalizes with ACTB at tricellular junctions between eye lens fiber cells.By similarity

GO - Cellular componenti

  • cell junction Source: UniProtKB-SubCell
  • cytoplasm Source: UniProtKB-SubCell
  • myelin sheath Source: RGD
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13831383PeriaxinPRO_0000058565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71PhosphoserineCombined sources
Modified residuei133 – 1331PhosphoserineCombined sources
Modified residuei243 – 2431PhosphoserineCombined sources
Modified residuei838 – 8381PhosphoserineCombined sources
Modified residuei971 – 9711PhosphoserineBy similarity
Modified residuei1020 – 10201PhosphoserineCombined sources
Modified residuei1271 – 12711PhosphoserineBy similarity
Modified residuei1275 – 12751PhosphoserineBy similarity
Modified residuei1277 – 12771PhosphoserineBy similarity
Modified residuei1285 – 12851PhosphoserineBy similarity
Modified residuei1323 – 13231PhosphoserineCombined sources
Modified residuei1329 – 13291PhosphoserineCombined sources
Modified residuei1361 – 13611PhosphoserineCombined sources

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63425.
PRIDEiQ63425.

PTM databases

iPTMnetiQ63425.
PhosphoSiteiQ63425.

Expressioni

Tissue specificityi

Detected in sciatic nerve and in trigeminal nerve Schwann cells (PubMed:11430802). Detected in myelinating Schwann cells in sciatic nerve (at protein level) (PubMed:8155317, PubMed:10671475).3 Publications

Developmental stagei

mRNA and protein levels peak in the sciatic nerve between posnatal days 8 and 20; thereafter they decline precipitously.1 Publication

Gene expression databases

BgeeiENSRNOG00000018369.

Interactioni

Subunit structurei

Homodimer (via PDZ domain) (PubMed:11430802). Interacts with SCN10A. Found in a complex with SCN10A (PubMed:12591166). Interacts with DRP2 (PubMed:11430802). Identified in a dystroglycan complex that contains at least PRX, DRP2, UTRN, DMD and DAG1 (By similarity). Detected in a complex composed of at least EZR, AHNAK, PPL and PRX (By similarity). Identified in a complex with EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, VIM and spectrin (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Scn10aQ629682EBI-1800492,EBI-1800320

Protein-protein interaction databases

IntActiQ63425. 1 interaction.
STRINGi10116.ENSRNOP00000024771.

Structurei

3D structure databases

ProteinModelPortaliQ63425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 9984PDZPROSITE-ProRule annotationAdd
BLAST
Repeati432 – 43651
Repeati440 – 44452
Repeati448 – 45253
Repeati456 – 46054
Repeati461 – 46555
Repeati466 – 47056
Repeati474 – 47857
Repeati482 – 48658
Repeati487 – 49159
Repeati492 – 496510
Repeati497 – 501511
Repeati502 – 506512
Repeati507 – 511513
Repeati515 – 519514
Repeati523 – 527515
Repeati531 – 535516
Repeati536 – 540517
Repeati544 – 548518
Repeati549 – 553519
Repeati554 – 558520
Repeati562 – 566521
Repeati567 – 571522
Repeati575 – 579523
Repeati580 – 584524
Repeati585 – 589525
Repeati593 – 597526
Repeati601 – 605527
Repeati606 – 610528
Repeati611 – 615529
Repeati619 – 623530
Repeati627 – 631531
Repeati632 – 636532
Repeati637 – 641533
Repeati645 – 649534
Repeati653 – 657535
Repeati658 – 662536
Repeati663 – 667537
Repeati671 – 675538
Repeati676 – 680539
Repeati684 – 688540
Repeati689 – 693541
Repeati694 – 698542
Repeati699 – 703543
Repeati705 – 709544
Repeati713 – 717545
Repeati718 – 722546

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni432 – 72229146 X 5 AA approximate tandem repeats of [LVMGIE]-[PSM]-[EDKA]-[LIVMA]-[AQKHPRT]; that may have a tripeptide spacer of [ALKD]-[IPV]-[KPH]Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi70 – 8415Nuclear export signalBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi118 – 19679Arg/Lys-rich (basic)Add
BLAST
Compositional biasi136 – 1394Poly-Lys
Compositional biasi208 – 2114Poly-Ala
Compositional biasi1036 – 1163128Glu-rich (acidic)Add
BLAST

Domaini

Has a remarkable domain of repetitive pentameric units sometimes followed by a tripeptide spacer, it may separate two functional basic and acidic domains.Curated
The Arg/Lys-rich basic domain functions as a tripartite nuclear localization signal.1 Publication
The PDZ domain contains the signal for export from the nucleus (By similarity). The N-terminal region including the PDZ domain is required for the formation of Cajal bands on myelinated nerves (By similarity).By similarity

Sequence similaritiesi

Belongs to the periaxin family.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410II3A. Eukaryota.
ENOG410ZMDY. LUCA.
HOGENOMiHOG000168246.
HOVERGENiHBG031836.
InParanoidiQ63425.
PhylomeDBiQ63425.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63425-1) [UniParc]FASTAAdd to basket
Also known as: L-periaxin

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEARSRSAEE LRRAELVEII VETEAQTGVS GFNVAGGGKE GIFVRELRED
60 70 80 90 100
SPAAKSLSLQ EGDQLLSARV FFENFKYEDA LRLLQCAEPY KVSFCLKRTV
110 120 130 140 150
PTGDLALRPG TVSGYEMKGP RAKVAKLNIQ SLSPVKKKKM VIGTLGTPAD
160 170 180 190 200
LAPVDVEFSF PKFSRLRRGL KADAVKGPVP AAPARRRLQL PRLRVREVAE
210 220 230 240 250
EAQVARMAAA APPSRKAKSE AEVATGAGFT APQIELVGPR LPSAEVGVPK
260 270 280 290 300
VSVPKGTPST EAASGFALHL PTLGLGAPAA PAVEPPTTGI QVPQVELPTL
310 320 330 340 350
PSLPTLPTLP CLDTQEGAAV VKVPTLDVAA PSVEVDLALP GAEVEAQGEV
360 370 380 390 400
PEVALKMPRL SFPRFGVRGK EATEAKVVKG SPEAKAKGPR LRMPTFGLSL
410 420 430 440 450
LESRPSGPEV AAESKLKLPT LKMPSFGISV AGPEVKAPKG PEVKLPKVPE
460 470 480 490 500
IKLPKAPEAA IPDVQLPEVQ LPKMSDMKLP KIPEMAVPDV HLPEVKLPKV
510 520 530 540 550
PEMKVPEMKL PKIPEMAVPD VHLPDIQLPK VPEMKLPDMK LPKVPEMAVP
560 570 580 590 600
DVHLPDIQLP KVPEMKLPDM KLPKVPEMAV PDVRIPEVQL PKVSEVKLPK
610 620 630 640 650
IPDMAVPDVR LPELQLPKMS EVKLPKIPDM AVPDVRLPEV QLPKVSELKL
660 670 680 690 700
PKVPEMTMPD IRLPEVQLPK VPDIKLPEIK LPKVPEMAVP DVPLPELQLP
710 720 730 740 750
KVPQVPDVHL PKVPEMKLPK VPEAQRKSAG AEQAEKTEFS FKLPKMTVPK
760 770 780 790 800
LGKVTKPGEA GIEVPDKLLI LPCLQPEVGT EVARVGVPSL SLPSVELDLP
810 820 830 840 850
GALGLEGQVQ EAVSGKVEKP EGPRVAVGTG EAGFRVPSVE IVNPQLPTVE
860 870 880 890 900
VKKEQLEMVE MKVKPTSKFS LPKFGLSGPK AVKAEVEGPG RATKLKVSKF
910 920 930 940 950
AISLPRARAG TDADAKGAGE AGLLPALDLS IPQLSLDAQL PSGKVEVAGA
960 970 980 990 1000
ESKPKGSRFA LPKFGAKGRD SEADVLVAGE AELEGKGWGW DGKVKMPKLK
1010 1020 1030 1040 1050
MPSFGLSRGK EAEIQDGRVS PGEKLEAIAG QLKIPEVELV TPGAQETEKV
1060 1070 1080 1090 1100
TSGVKPSGLQ VSTTRQVVAE GQEGAQRVSS LGISLPQVEL ASFGEAGPEI
1110 1120 1130 1140 1150
AAPSAEGTVG SRIQVPQVML ELPGTQVAGG DLLVGEGIFK MPTVTVPQLE
1160 1170 1180 1190 1200
LDVGLGHEAQ AGETAKSEGG LKLKLPTLGA GGKGEGAEAQ SPEAQHTFHI
1210 1220 1230 1240 1250
SLPDVELTSP VSSHAEYQVV EGDGDGGHKL KVRLPLFGLA RAKEGIETGE
1260 1270 1280 1290 1300
KVKSPKLRLP RVGFSQSESA SGEGSPSPEE EEEGSGEGAS GRRGRVRVRL
1310 1320 1330 1340 1350
PRVGLASPSK GSKGQEGDAA SKSPVGEKSP KFRFPRVSLS PKARSGSKDR
1360 1370 1380
EEGGFRVRLP SVGFSETAAP GSARIEGTQA AAI
Length:1,383
Mass (Da):146,400
Last modified:April 16, 2002 - v2
Checksum:iFF6593BCFA23A437
GO
Isoform 2 (identifier: Q63425-2) [UniParc]FASTAAdd to basket
Also known as: S-periaxin

The sequence of this isoform differs from the canonical sequence as follows:
     128-147: NIQSLSPVKKKKMVIGTLGT → VRVLSPVPVQDSPSDAVAAP
     148-1383: Missing.

Show »
Length:147
Mass (Da):15,968
Checksum:i17A1489927A9B022
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei128 – 14720NIQSL…GTLGT → VRVLSPVPVQDSPSDAVAAP in isoform 2. 1 PublicationVSP_004368Add
BLAST
Alternative sequencei148 – 13831236Missing in isoform 2. 1 PublicationVSP_004369Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29649 mRNA. Translation: CAA82757.2.
PIRiI58157.
RefSeqiNP_076466.2. NM_023976.2. [Q63425-1]
UniGeneiRn.10395.

Genome annotation databases

GeneIDi78960.
KEGGirno:78960.
UCSCiRGD:619960. rat. [Q63425-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29649 mRNA. Translation: CAA82757.2.
PIRiI58157.
RefSeqiNP_076466.2. NM_023976.2. [Q63425-1]
UniGeneiRn.10395.

3D structure databases

ProteinModelPortaliQ63425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63425. 1 interaction.
STRINGi10116.ENSRNOP00000024771.

PTM databases

iPTMnetiQ63425.
PhosphoSiteiQ63425.

Proteomic databases

PaxDbiQ63425.
PRIDEiQ63425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi78960.
KEGGirno:78960.
UCSCiRGD:619960. rat. [Q63425-1]

Organism-specific databases

CTDi57716.
RGDi619960. Prx.

Phylogenomic databases

eggNOGiENOG410II3A. Eukaryota.
ENOG410ZMDY. LUCA.
HOGENOMiHOG000168246.
HOVERGENiHBG031836.
InParanoidiQ63425.
PhylomeDBiQ63425.

Miscellaneous databases

PROiQ63425.

Gene expression databases

BgeeiENSRNOG00000018369.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRAX_RAT
AccessioniPrimary (citable) accession number: Q63425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 16, 2002
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.